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- 저자 : 김현원 ( Hyun-won Kim ) (검색결과 6 건)
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Purification and Characterization of $\beta$-Glucuronidase from Rat Uterus.
김현원,양철학,Kim, Hyun-Won,Yang, Chul-Hak 생화학분자생물학회 1983 한국생화학회지 Vol.16 No.2
쥐의 자궁에서 베타-글룩유로니다아제를 $(NH_4)_2S0_4$ 분획분리, DEAE-셀루로오즈 크로마토그라피법, 젤여과법 및 Concanavalin-A Sepharose 흡착 크로마토그라피법등을 사용하여 완전분리 정제 하였다. 정제된 효소는 pH 8.3, 7.5% 아크릴아마이드 젤에서 전기영동한 결과 단일 단백질로 나타났다. 젤여과법에 의해 얻어진 분자량은 288,000 Dalton 이었고 SDS 폴리아크릴아마이드 젤 전기 영동법에 의해 이 효소는 75,000의 분자량을 갖는 단위 조각으로 구성 됐음을 알아냈다. 이 효소의 에 따른 활동도는 기질의 농도나 이온 강도의 변화에 따라 변화됨을 볼 수 있었다. 또한 이 효소는 중성보다 산성 완충용액일때 비교적 높은 온도에서 더욱 안정하였다. P-Nitrophenyl-$\beta$-D-glucuronide를 기질로 사용하였을 Km 치는 0.25mM 이고 $Hg^{++}$, $Cu^{++}$, $Ag^{+}$ 같은 중금속 이온에 의해 효소의 활동도가 방해 되었다. citrate, heparin 이나 식초산도 역시 효소의 활동도를 방해 하였다. 이온강도가 0.4에서 여러 염들도 효소의 활동도를 20%정도감소시켰다. $\beta$-Glucuronidase was purified from rat uter us by $(NH_4)_2S0_4$ fractionation, DEAE-cellulose chromatography, gel filtration on Sephadex G-200, CM-cellulose chromatography, and affinity chromatography on Concanavalin-A Sepharose. The purified enzyme appeares homogeneous on electrophoresis in a 7.5% polyacrylamide gel at pH 8.3, and had a molecular weight of 288,000 daltons by gel filtration. SDS polyacrylamide gel electrophoresis indicated that enzyme consisted of subunit with molecular weight of 75,000. The pH profile of the enyzme varied when changing substrate concentration and ionic strength. The enzyme was more stable at high temperature in acidic buffers than in neutral ones. The Km value for p-nitrophenyl-$\beta$-D-glucuronide as substrate was 0.25 mM, and the enzyme was inhibited by heavy metal ions, such as $Hg^{2+}$, $Cu^{2+}$ and $Ag^+$. Citrate, heparin, and acetate also inhibited the enzeme. Various salts decrease the enzyme activity by 20% at ionic strength of 0.4.
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쥐의 자궁으로 부터 β - Glucuronidase 의 분리정제와 효소특성에 관한 연구
김현원,양철학 ( Hyun Won Kim,Chul Hak Yang ) 생화학분자생물학회 1983 BMB Reports Vol.16 No.2
β-Glucuronidase was purified from rat uter us by (NH₄)₂SO₄ fractionation, DEAE-cellulose chromatography, gel filtration on Sephadex G-200, CM-cellulose chromatography, and affinity chromatography on Concanavalin-A Sepharose. The purified enzyme appeares homogeneous on electrophoresis in a 7.5 % polyacrylamide gel at pH 8.3, and had a molecular weight of 288,000 daltons by gel filtration. SDS polyacrylamide gel electrophoresis indicated that enzyme consisted of subunit with molecular weight of 75, 000. The pH profile of the enyzme varied when changing substrate concentration and ionic strength. The enzyme was more stable at high temperature in acidic buffers than in neutral ones. The Km value for p-nitrophenyl-β-D-glucuronide as substrate was 0. 25 mM, and the enzyme was inhibited by heavy metal ions, such as Hg^(2+), Cu^(2+) and Ag^+. Citrate, heparin, and acetate also inhibited the enzeme. Various salts decrease the enzyme activity by 20 % at ionic strength of 0.4.
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토끼 복부 내벽으로부터 분리된 경락으로 믿어지는 관조직
손준형 ( Joon Hyung Sohn ),윤진하 ( Jin-ha Yoon ),김영주 ( Young Joo Kim ),김민경 ( Min Kyung Kim ),김지화 ( Ji Hwa Kim ),권오현 ( Ohyun Kwon ),김현원 ( Hyun-won Kim ) 대한미용학회(구 대한미용과학회) 2016 대한미용학회지 Vol.12 No.4
Investigation of meridian line and acupoints as anatomical entities should be very important, as acupuncture and meridian massage for medical purpose in the hospital or for skin care in beauty shop are each based on the traditional meridian theory. We isolated tubular structures under the skin of rabbits where the traditional meridian lines are located. The characteristics of these tubular structures isolated from the abdominal wall matches those of Bonghan tubes and Bonghan tubules found by Bong Han Kim. The Bonghan theory established by Bong Han Kim suggests that meridian lines consist of Bonghan tubes and Bonghan tubules. We could observe the presence of large cells inside the Bonghan tubule and the presence of small granules referred to as Sanal in Bonghan theory inside the striae consisting of the Bonghan tubules. Present research suggests that there exist a third unique tubular structure where Sanals flow other than Bonghan tube and Bonghan tubules.
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Alkaline reduced water produced by mineQ showed anti-cancer and anti-diabetic effect
Hyun-Won Kim(김현원) 한국정신과학학회 2007 한국정신과학회 학술대회 논문집 Vol.27 No.-
Special mineral combination(mineQ) was developed to produce alkaline reduced water(ARW) with high pH and low oxidation-reduction potential (ORP) and with the torsion information. ARW produced by mineQ showed anti-diabetic effectand anti-cancer effect. ARW spurs the growth of Otsuka Long-Evans Tokushima Fatty (OLETF) rats with spontaneous development of diabetic conditions, and long-term ingestion of ARW resulted in a reduction in the levels of glucose, triglycerides, and total cholesterol in the blood. When B16 melanoma cells were inoculated subcutaneously and intra-peritoneally, C56BL/6 mice fed with ARW showed tumor growth delay and thesurvival span was significantly lengthened. ARW also showed the inhibition of metastasis by reducing the numbers of B16 melanoma colonies when injected through tail vein. The amount of reactive oxygen species (ROS) was reduced when fed with ARW except for spleen, whichis a major organ for immunity. Even for normal mice, ARW intake invoked systemic cytokines, such as, Th1. (IFN-g, IL-12) and Th2 (IL-4, IL-5), suggesting strong immuno-modulation effect. Both ROS scavenging effect and immuno-modulation effect might be responsible for anti-cancer and anti-diabetic effect of alkaline reduced water.
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<sup>99m</sup>Tc를 이용한 경락의 위치와 흐름의 추적
손준형 ( Joon Hyung Sohn ),김현원 ( Hyun-won Kim ) 대한미용학회(구 대한미용과학회) 2016 대한미용학회지 Vol.12 No.3
Investigation of meridian line and acupoints should be very important, as acupuncture and meridian massage for medical purpose in the hospital or for skin care in beauty shop are all based on traditional meridian theory. Radioactive technetium-99m pertechnetate (<sup>99m</sup>Tc) injected to a specific acupoint flowed upward along the meridian line as was suggested by traditional meridian theory. Tracing of injected <sup>99m</sup>Tc to an acupoint revealed that the meridian is a unique ductal system through which the materials flow.
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