Understanding the molecular interaction and relaxation findings in amphiphilics on solution state using TDR

Senthilkumar P.,Vinoth K.,Maria Sylvester M.,Karunakaran D.J.S.Anand,Deshmukh Avadhut Ramrao,Hudge Pravin,Ganesh T.,Kumbharkhane A.C. 한국물리학회 2022 Current Applied Physics Vol.38 No.-

The dielectric behavior of stearic acid in 1,4-dioxane medium at various temperature 303 K–288 K and frequency regime (10 MHz–30 GHz) determined from the complex dielectric permittivity spectra obtained by Time Domain Reflectometry(TDR). In this frequency range, the dielectric study gives the electrostatic interaction as well as orientational polarizability of complex mixtures of long chain molecules significantly. Dielectric parameters were calculated from the complex spectra of the binary mixture by non linear least square fit method. The excess permittivity (εE), correlation factors were calculated for the binary system. Thermal parameters(ΔH-enthalpy, ΔS-entropy and ΔG-Gibbs free energy) were calculated and the direction of reaction is determined. The FTIR spectrum of the binary system recorded and the assignments are discussed. The FTIR spectral assignments confirm the molecular interactions.

Sustained Release of Rottlerin Encapsulated within Poly(D,L-Lactic-co-Glycolic Acid) Nanoparticles Inhibits Migration and Clonogenicity in Pancreatic Cancer Cells

Snima, K. S.,Nair, Shantikumar V.,Lakshmanan, Vinoth-Kumar American Scientific Publishers 2016 Journal of nanoscience and nanotechnology Vol.16 No.8

<P>In our present study, we focused on the preparation and evaluation of a stable nano-formulation of small molecule drug rottlerin encapsulated within PLGA (Poly(D,L-lactic-co-glycolic acid)) nanoparticles. The endeavor was to increase therapeutic efficacy of rottlerin against, and drug delivery to, pancreatic cancer cells. Size and morphology analysis by Dynamic light scattering (DLS) and Scanning electron microscopy (SEM) revealed the average size of nanoparticles prepared was 150-300 nm. In vitro drug release study confirmed the suitability of PLGA nanoparticles as a matrix for controlled release of rottlerin. In vitro cytotoxicity assay in MiaPaCa-2 cells showed dose dependent and preferential toxicity on pancreatic cancer cells compared to normal Vero cells. Furthermore, apoptosis mediated cell death was higher in the rottlerin nanoparticles treated cells and there was also a significant delay in cancer cell migration along with reduction in colony formation. Henceforth the study prospects that the potential therapeutic value of rottlerin against pancreatic cancer can have added advantage through its nanoformulation using a suitable polymeric carrier.</P>

Effect of Prunetin on Streptozotocin-Induced Diabetic Nephropathy in Rats - a Biochemical and Molecular Approach

Samy Jose Vinoth Raja Antony,Kumar Nirubama,Singaravel Sengottuvelu,Krishnamoorthy Rajapandiyan,Alshuniaber Mohammad A,Gatasheh Mansour K.,Venkatesan Amalan,Natesan Vijayakumar,Kim Sung-Jin 한국응용약물학회 2023 Biomolecules & Therapeutics(구 응용약물학회지) Vol.31 No.6

In the modern era, chronic kidney failure due to diabetes has spread across the globe. Prunetin (PRU), a component of herbal medicines, has a broad variety of pharmacological activities; these may help to slow the onset of diabetic kidney disease. The anti-nephropathic effects of PRU have not yet been reported. The present study explored the potential nephroprotective actions of PRU in diabetic rats. For 28 days, nephropathic rats were given oral doses of PRU (20, 40, and 80 mg/kg). Body weight, blood urea, creatinine, total protein, lipid profile, liver marker enzymes, carbohydrate metabolic enzymes, C-reactive protein, antioxidants, lipid peroxidative indicators, and the expression of insulin receptor substrate 1 (IRS-1) and glucose transporter 2 (GLUT-2) mRNA genes were all examined. Histological examinations of the kidneys, liver, and pancreas were also performed. The oral treatment of PRU drastically lowered the blood glucose, HbA1c, blood urea, creatinine, serum glutamic-oxaloacetic transaminase, serum glutamic pyruvic transaminase, alkaline phosphatase, lipid profile, and hexokinase. Meanwhile, the levels of fructose 1,6-bisphosphatase, glucose-6-phosphatase, and phosphoenol pyruvate carboxykinase were all elevated, but glucose- 6-phosphate dehydrogenase dropped significantly. Inflammatory marker antioxidants and lipid peroxidative markers were also less persistent due to this administration. PRU upregulated the IRS-1 and GLUT-2 gene expression in the nephropathic group. The possible renoprotective properties of PRU were validated by histopathology of the liver, kidney, and pancreatic tissues. It is therefore proposed that PRU (80 mg/kg) has considerable renoprotective benefits in diabetic nephropathy in rats.

Assessment of Cyclic Load Induced Energy Dissipation and Damping on GFRP Composite Laminate

T. G. Loganathan,K. Vinoth Kumar,R. Krishnamurthy 한국섬유공학회 2020 Fibers and polymers Vol.21 No.9

Polymeric composites exhibit load sensitive stiffness unlike the case of homogeneous metallic material. Composites are widely used in dynamic loading environment and hence it is necessary to study their response in terms ofstructural properties. Behavioural changes of glass epoxy composite laminate on exposure to cyclic loading has been assessedin terms of energy dissipation (Ed) and Damping factor (DF) by hysteresis loop. GFRP composite specimens (UD-0, 0/30/60/0, 0/45/0/-45, 0/90/90/0, and 0/90/0/90) are exposed to low velocity constant amplitude cyclic loading using a laboratoryarrangement (by an eccentric disc) at 4.6 Hz and 8.6 Hz frequencies. In fibre-reinforced composites apart from the fibrevolume fraction, the fibre interaction angle significantly influences their dynamic properties on loading. Unidirectional (UD-0) laminate exhibits low damping/energy dissipation, while 0/90/0/90 laminate with large fibre interaction angle showshighest damping/energy dissipation. Whereas, symmetric cross ply (0/90/90/0) laminate acts as a performance demarcationamong the chosen laminates. Thus, optimum Ed/DF properties of GFRP laminate in dynamic environment is attributed tosymmetric lay-up, smaller fibre orientation interaction angle in the lay-up sequence and 0 fibre layer at the boundary.

Properties of Heparinoids Premixed with Tumor-Derived Extracellular Vesicles

Manandhar, Sumeet,Park, Jooho,Kothandan, Vinoth K.,Lee, Joomin,Alam, Farzana,Jee, Jun-Pil,Hwang, Jinsu,Byun, Youngro,Hwang, Seung Rim American Chemical Society 2018 Bioconjugate chemistry Vol.29 No.11

<P>Tumor-derived exosomes are bound and internalized to organ-specific cells, affecting metastasis. Heparan sulfate proteoglycans mediate the interaction between cells and exosomes. Exosome transfer to the recipient cell can be competitively blocked by heparinoids, because heparin is structurally similar to heparan sulfate. It is hypothesized that there may be structural requirements of heparinoids to attenuate the cellular uptake and metastatic activity of tumor-derived exosomes. Here, we compared the properties of unfractionated heparin (UFH), glycol-split UFH, low-molecular-weight heparin (LMWH), glycol-split LMWH, and ultra-LMWH premixed with A549-derived exosomes. Uptake of A549-derived exosomes (0.1 mg/mL) into BEAS-2B cells was significantly blocked by 0.4 mg/mL of heparinoids. Heparinoids attenuated migration of BEAS-2B cells stimulated by A549-derived exosomes. Glycol-split LMWH with no antifactor Xa activity exhibited the strongest antimigratory effects than other heparinoids. Thus, heparinoids with proper molecular weight and structure can inhibit tumor-derived exosomes, not proportionally to the anticoagulant activity.</P> [FIG OMISSION]</BR>

Implementation of a BCF Mode Bio-mimetic Robotic-Fish Underwater Vehicle based on Lighthill Mathematical Model

Abhra Roy Chowdhury,Bhuneshwar Prasad,Vinoth Vishwanathan,Rajesh Kumar,S. K. Panda 제어로봇시스템학회 2012 제어로봇시스템학회 국제학술대회 논문집 Vol.2012 No.10

The objective of this paper is to mimic the propulsion mechanism of the BCF mode carangiform swimming style to show the fish behavior navigating efficiently over large distances at impressive speeds and its exceptional characteristics in the fluid environment. The robofish model (kinematics and dynamics) is integrated with the Lighthill(LH) mathematical model framework. Comparative studies are undertaken between a LH model based and a non-LH based model. A comprehensive propulsion mechanism study of the different parameters namely the tailbeat frequency (TBF), the propulsive wavelength and the caudal amplitude are studied under this framework. The performance factor taken for energy efficient swimming is the overall distance traversed. Inverse kinematics based approach is incorporated for trajectory generation of the robotic fish vehicle motion. Analysis of these critical parameters affecting the kinematics study of the vehicle vis a vis the real fish kinematic study [8] is carried out for a given trajectory. TBF is found to be the effective controlling parameter for the forward speed of the vehicle over a wide operating conditions. Performances and comparative results of propulsive wavelength and amplitude affects are also shown and discussed.