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Regeneration of Plants from EMS-treated Immature Embryo Cultures in Soybean [Glycine max(L.) Merr.]
Van, Kyu-Jung,Jang, Hyun-Ju,Jang, Young-Eun,Lee, Suk-Ha The Korean Society of Crop Science 2008 Journal of crop science and biotechnology Vol.11 No.2
Since somatic embryogenesis combined with ethylmethane sulfonate(EMS) treatments is the most efficient technique for mutagenesis, the embryogenic capacity of four soybean cultivars was evaluated at different EMS concentrations, treatment times, and preculture durations. Two to 4 mm long immature cotyledons were placed in induction medium after EMS treatment, and the numbers of somatic embryos formed per explant were counted four weeks after culture initiation. We observed genotypic differences in the efficiency of somatic embryogenesis from immature embryos among four cultivars treated with different concentrations of EMS for six hours. Cultivars, Sinpaldalkong 2 and Jack, displayed highly efficient somatic embryogenesis regardless of EMS concentration, whereas very low efficiency or no survival was observed in Jinju 1 and Iksannamulkong cultivars. Preculture duration did not influence the efficiency of somatic embryogenesis. Because Sinpaldalkong 2 exhibited the best somatic embryogenesis, much higher concentrations of EMS were used to test somatic embryo formation under different periods of time in this cultivar. Three and six hour treatments with both 1 and 2 mM EMS yielded higher embryo formation than longer periods of time. Increasing the time with embryos in 2 mM EMS caused a reduction in somatic embryogenesis in Sinpaldalkong 2, but many chlorophyll-deficient soybean variants were identified in the $M_1R_0$ and $M_2R_1$ generations. In addition to Jack, Sinpaldalkong 2 is a good genotype for plant regeneration from EMS-treated immature embryo cultures.
Molecular Characterization of Hypernodulation in Soybean
Van, Kyu-Jung,Ha, Bo-Keun,Hwang, Eun-Young,Kim, Moon-Young,Heu, Sung-Gi,Lee, Suk-Ha The Korean Society of Plant Pathology 2003 Plant Pathology Journal Vol.19 No.1
SS2-2, a hypernodulating soybean mutant was isolated by EMS mutagenesis from Sinpaldalkong 2. This auto-regulation mutant showed greater number of nodules and smaller plant size than its wild type Sinpaldalkong 2. SSR markers were used to identify DNA variation at SSR loci from different soybean LG. The only SSR marker that detected a length polymorphism between SS2-2 and its wild type ancestor was Satt294 on LG C1 instead of LG H, locating a hypernodulating gene. Sequencing data of flanking Satt294 indicated that the size variation was due to extra stretch of TTA repeats of the SSR motif in SS2-2, along with $A\longrightarrow$G transversion. In spite of phenotypic differences between the wild type and its hypernodulating mutants, genomic DNA poly-morphisms at microsatellite loci could not control regulation of nodule formation. The cDNA-AFLP method was applied to compare differential display of cDNA between Sinpaldalkong 2 and SS2-2. After isolation and sequence comparison with many AELP fragments, several interesting genes were identified. Northern blot analysis, immunolocalization and/or the yeast two-hybrid system with these genes might provide information on regulation of nodule development in SS2-2.
( Trinh Van Trung ),( Sun Kyu Kim ),( Min Jung Kim ),( Seul Ki Kim ),( Sung Jun Bong ),( Dong Bok Lee ) 대한금속재료학회 ( 구 대한금속학회 ) 2012 대한금속·재료학회지 Vol.50 No.8
Medium carbon steel was aluminized by hot dipping into molten Al or Al-1 at% Si baths. After hot-dipping in these baths, a thin Al-rich topcoat and a thick alloy layer rich in Al5Fe2 formed on the surface. A small amount of FeAl and Al3Fe was incorporated in the alloy layer. Silicon from the Al-1 at% Si bath was uniformly distributed throughout the entire coating. The hot dipping increased the microhardness of the steel by about 8 times. Heating at 700-1000, however, decreased the microhardness through interdiffusion between the coating and the substrate. The oxidation at 700-1000 in air formed a thin protective a-Al2O3 layer, which provided good oxidation resistance. Silicon was oxidized to amorphous silica, exhibiting a glassy oxide surface.
Study on the Constituents of Roots of Aceriphyllum rossii
Kim Van, Le Thi,Hung, Tran Manh,Kim, Soo Hyun,Kim, Jin Cheol,Na, MinKyun,Jung, Hyun Ju,Kwack, Seung Jun,Kweon, Kee Tae,Choi, Jae Sue,Lee, Hyeong Kyu,Bae, KiHwan,Min, Byung-Sun WILEY-VCH Verlag 2010 Helvetica chimica acta Vol.93 No.9
<P>A new stereoisomer of a tetrahydrofuranoid lignan, acerifuranoid A (1), and two new oleanane-type triterpenoids, aceriphyllic acids J and K (2 and 3), were isolated from the roots of Aceriphyllum rossii. Their structures were elucidated on the basis of spectroscopic analyses and chemical evidence. These isolated compounds exhibited weak cytotoxic activity against various cancer cell lines with IC<SUB>50</SUB>>150 μM.</P> <B>Graphic Abstract</B> <P> <img src='wiley_img_2010/0018019X-2010-93-9-HLCA200900467-content.gif' alt='wiley_img_2010/0018019X-2010-93-9-HLCA200900467-content'> </P>
Detection of unusual rotavirus genotypes G8P[8] and G12P[6] in South Korea
Le, Van Phan,Kim, Ji-Yeon,Cho, Sung-Lim,Nam, Sun-Woo,Lim, Inseok,Lee, Hee-Joong,Kim, Kijeong,Chung, Sang-In,Song, Wonkeun,Lee, Kyu Man,Rhee, Moon-Soo,Lee, Jung-Sook,Kim, Wonyong Wiley Subscription Services, Inc., A Wiley Company 2008 Journal of medical virology Vol.80 No.1
<P>Five hundred four fecal specimens, collected between 2004 and 2006 from young children with acute diarrhea, were screened for rotavirus by ELISA with VP6-specific antibody. Of these samples, 394 (78.2%) were confirmed as group A rotavirus and they underwent G- and P typing using a combination of ELISA, RT-PCR, and sequence analysis methods. The dominant circulating G serotype was G1 (35.6%) followed by G3 (26.4%), G4 (14.7%), and G2 (11.9%). There was a low prevalence of G9 (1.0%) and of unusual G type rotavirus, in particular, G12 (0.5%) and G8 (0.3%). Of the P genotype rotavirus in circulation, P[8] (53.0%) was most common followed by P[6] (15.5%), P[4] (15.2%), and P[9] (2.3%). Determination of G- and P type combinations revealed that G1P[8] strains were most prevalent (25.4%), amid G3P[8] (16.8%), G2P[4] (6.3%), and G4P[6] (6.1%) strains. Unusual or rare combinations such as G2P[6], G2P[8], G3P[4], G2P[9], G1P[9], G3P[9], G12P[6], G1P[4], G3P[6], and G8P[8] were also found. Owing to the recent emergence of G8 and G12 rotavirus, the findings from this study are important since they provide new information concerning the local and global spread of rotavirus genotypes. J. Med. Virol. 80:175–182, 2008. © 2007 Wiley-Liss, Inc.</P>
Evaluation of Soybean Cultivars for Resistance to Phomopsis Seed Decay in Korea
Sun, Suli,Van, Kyu-Jung,Kim, Moon-Young,Lee, Yeong-Ho,Ko, Jong-Min,Baek, In-Youl,Lee, Yin-Won,Liu, Weixian,Lee, Suk-Ha 한국작물학회 2012 Journal of crop science and biotechnology Vol.15 No.2
Phomopsis seed decay (PSD), primarily caused by Phomopsis longicolla, is one of the most important seed-borne diseases and causes serious seed yield loss in soybean. This study was performed to evaluate reactions to P. longicolla in Korean soybean major elite cultivars, which were mainly used for parents of genetic mapping populations. The natural incidence of P. longicolla and other seed-borne fungi was determined in the fields at three different locations in South Korea during 2009 - 2010. The significant differences in sensitivity to seed-borne diseases were shown among cultivars. Taekwangkong exhibited the greatest resistance to P. longicolla with average incidence of 0.33% and other seed-borne fungi with average incidence of 6.17%. Moreover, Taekwangkong was free of P. longicolla infection both in Milyang and in Daegu. To confirm the effective resistance source, the Korean virulent strain of P. longicolla, SSLP-3, was inoculated artificially on soybean of R4-R7 growth stage in the greenhouse. Taekwangkong exhibited a higher level of resistance to P. longicolla with significantly lower incidence (8.67%) than any other Korean elite cultivars (78.0 - 99.33%) and the previously reported resistant PI genotypes (35.0 - 55.67%). Further verification of resistance in Taekwangkong to P. longicolla by testing germination vigor of healthy seeds in vitro showed a higher germination rate than those of the susceptible cultivars. It could be suggested that Taekwangkong is a newly identified resistance source and the better source of resistance to P. longicolla to develop breeding populations for exploiting resistance gene(s) in further studies.