http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
최영준,Marco Thines,Jae-Gu Han,신현동 한국미생물학회 2011 The journal of microbiology Vol.49 No.6
Since about two hundred years, downy mildew caused by Peronospora effusa is probably the most economically important disease of spinach (Spinacia oleracea). However, there is no information on the global phylogeographic structure of the pathogen and thus it is unclear whether a single genotype occurs worldwide or whether some local genetic variation exists. To investigate the genetic variability of this pathogen, a sequence analysis of two partial mitochondrial DNA genes, cox2 and nad1, was carried out. Thirty-three specimens of Peronospora effusa from four continents were analyzed, including samples from Australia, China, Japan, Korea, Mexico, Russia, Sweden, and the USA. Despite the potential anthropogenic admixture of genotypes, a phylogeographic pattern was observed, which corresponds to two major groups, an Asian/Oceanian clade and another group, which includes American/European specimens. Notably, two of six Japanese specimens investigated did not belong to the Asian/Oceanian clade, but were identical to three of the specimens from the USA, suggestive of a recent introduction from the USA to Japan. As similar introduction events may be occurring as a result of the globalised trade with plant and seed material, a better knowledge of the phylogeographic distribution of pathogens is highly warranted for food security purposes.
Mandaokar, Ajin,Thines, Bryan,Shin, Byongchul,Markus Lange, B.,Choi, Goh,Koo, Yeon J.,Yoo, Yung J.,Choi, Yang D.,Choi, Giltsu,Browse, John Blackwell Publishing Ltd 2006 The Plant journal Vol.46 No.6
<P>Summary</P><P>In Arabidopsis, jasmonate is required for stamen and pollen maturation. Mutants deficient in jasmonate synthesis, such as <I>opr3</I>, are male-sterile but become fertile when jasmonate is applied to developing flower buds. We have used ATH1 oligonucleotide arrays to follow gene expression in <I>opr3</I> stamens for 22 h following jasmonate treatment. In these experiments, a total of 821 genes were specifically induced by jasmonate and 480 genes were repressed. Comparisons with data from previous studies indicate that these genes constitute a stamen-specific jasmonate transcriptome, with a large proportion (70%) of the genes expressed in the sporophytic tissue but not in the pollen. Bioinformatics tools allowed us to associate many of the induced genes with metabolic pathways that are probably upregulated during jasmonate-induced maturation. Our pathway analysis led to the identification of specific genes within larger families of homologues that apparently encode stamen-specific isozymes. Extensive additional analysis of our dataset identified 13 transcription factors that may be key regulators of the stamen maturation processes triggered by jasmonate. Two of these transcription factors, MYB21 and MYB24, are the only members of subgroup 19 of the R2R3 family of MYB proteins. A <I>myb21</I> mutant obtained by reverse genetics exhibited shorter anther filaments, delayed anther dehiscence and greatly reduced male fertility. A <I>myb24</I> mutant was phenotypically wild-type, but production of a <I>myb21</I><I>myb24</I> double mutant indicated that introduction of the <I>myb24</I> mutation exacerbated all three aspects of the <I>myb21</I> phenotype. Exogenous jasmonate could not restore fertility to <I>myb21</I> or <I>myb21</I><I>myb24</I> mutant plants. Together with the data from transcriptional profiling, these results indicate that MYB21 and MYB24 are induced by jasmonate and mediate important aspects of the jasmonate response during stamen development.</P>
Adsorption of chromium (VI) on functionalized and non-functionalized carbon nanotubes
Nabisab Mujawar Mubarak,Raj Kogiladas Thines,Noor Rosyidah Sajuni,Ezzat Chan Abdulla,Jaya Narayan Sahu,Poobalan Ganesan,Natesan Subramanian Jayakumar 한국화학공학회 2014 Korean Journal of Chemical Engineering Vol.31 No.9
We did a comparative study on the adsorption capacity of Cr (VI) between functionalized carbon nanotubes(CNTs) and non-functionalized CNTs. The statistical analysis reveals that the optimum conditions for the highest removalof Cr (VI) are at pH 9, with dosage 0.1 gram, agitation speed and time of 120 rpm and 120 minutes, respectively. Forthe initial concentration of 1.0 mg/l, the removal efficiency of Cr (VI) using functionalized CNTs was 87.6% and 83%of non-functionalized CNTs. The maximum adsorption capacities of functionalized and non–functionalized CNTs were2.517 and 2.49 mg/g, respectively. Langmuir and Freundlich models were adopted to study the adsorption isotherm,which provided a KL and KF value of 1.217 L/mg and 18.14 mg1−nLn/g functionalized CNT, while 2.365 L/mg and 2.307mg1−nLn/g for non-functionalized CNTs. This result proves that functionalized CNTs are a better adsorbent with a higheradsorption capacity compared with the non-functionalized CNTs.
A New Marine Species of Miracula (Oomycota) Parasitic to Minidiscus sp. in Iceland
( Anthony T. Buaya ),( Bettina Scholz ),( Marco Thines ) 한국균학회 2021 Mycobiology Vol.49 No.4
Obligate endoparasitic oomycetes are known to ubiquitously occur in marine and freshwater diatoms, but their diversity is still largely unexplored. Many of these parasitoids are members of the early-diverging oomycete lineages (Miracula, Diatomophthora), others are within the Leptomitales of the Saprolegniomycetes (Ectrogella, Lagenisma) and some have been described in the Peronosporomycetes (Aphanomycopsis, Lagenidium). Even though some species have been recently described and two new genera were introduced (Miracula and Diatomophthora), the phylogeny and taxonomy of most of these organisms remain unresolved. This is contrasted by the high number of sequences from unclassified species, as recently revealed from environmental sequencing, suggesting the presence of several undiscovered species. In this study, a new species of Miracula is reported from a marine centric diatom (Minidiscus sp.) isolated from Skagastr€ond harbor in Northwest Iceland. The morphology and life cycle traits of this novel oomycete parasite are described herein, and its taxonomic placement within the genus Miracula is confirmed by molecular phylogeny. As it cannot be assigned to any previously described species, it is introduced as Miracula islandica in this study. The genus Miracula thus contains three described holocarpic species (M. helgolandica, M. islandica, M. moenusica) to which likely additional species will need to be added in the future, considering the presence of several lineages known only from environmental sequencing that clustered within the Miracula clade.
Manimaran Ruthiraan,Nabisab Mujawar Mubarak,Raj Kogiladas Thines,Ezzat Chan Abdullah,Jaya Narayan Sahu,Natesan Subramanian Jayakumar,Poobalan Ganesan 한국화학공학회 2015 Korean Journal of Chemical Engineering Vol.32 No.3
We did a comparative study between functionalized multiwall carbon nanotube (FMWCNTs), and magneticbiochar was carried out to determine the most efficient adsorbent to be employed in the Cd2+ ion removal. Weoptimized parameters such as agitation speed, contact time, pH and adsorbent dosage using design expert vrsion 6.08. The statistical analysis reveals that optimized condition for highest removal of Cd2+ are at pH 5.0, with dosage 1.0 g,agitation speed and contact time of 100 rpm and 90 minutes, respectively. For the initial concentration of 10mg/l, theremoval efficiency of Cd2+ using FMWCNTs was 90% and and 82% of magnetic biochar. The maximum Cd2+ adsorptioncapacities of both FMWCNTs and magnetic biochar were calculated: 83.33mg/g and 62.5mg/g. The Langmuirand Freundlich constants for FMWCNTs were 0.056 L/mg and 13.613 L/mg, while 0.098 L/mg and 25.204 L/mg formagnetic biochar. The statistical analysis proved that FMWCNTs have better adsorption capacity compared to magneticbiochar and both models obeyed the pseudo-second-order.
( Young-joon Choi ),( Marlena Görg ),( Hyeon-dong Shin ),( Marco Thines ) 한국균학회 2020 Mycobiology Vol.48 No.4
Over the past 15 years, downy mildew became the most destructive foliar disease in cultivated Impatiens species (Balsaminaceae) worldwide. A previous study had revealed that the causal agent was not Plasmopara obducens (Oomycota, Peronosporales) but Plasmopara destructor on Impatiens walleriana, and Plasmopara velutina on Impatiens balsamina. This hints to a relatively high degree of specialization of Plasmopara on Balsaminaceae. Therefore, it was the aim of the present study to perform multigene phylogenetic analysis and detailed morphological investigation for several Korean downy mildew samples parasitic to cultivated I. walleriana, and I. balsamina, but also to a northeast Asian wild plant, Impatiens textori. It was revealed that I. textori harbors a new species, which is introduced and described here as Plasmopara elegantissima.