Electrochemical detection of DNA mutations on a PNA-modified electrode utilizing a single-stranded DNA specific endonuclease

Shin, Sujeong,Won, Byoung Yeon,Jung, Cheulhee,Shin, Sung Chul,Cho, Dae Yeon,Lee, Soo Suk,Park, Hyun Gyu Royal Society of Chemistry 2011 Chemical communications Vol.47 No.23

<P>Utilizing a peptide nucleic acid (PNA)-modified electrode and a single-stranded DNA specific endonuclease, a novel electrochemical method to identify DNA mutations has been developed and represents a totally new strategy for the electrochemical diagnosis of human genetic mutations.</P> <P>Graphic Abstract</P><P>Only a base-mismatched site formed in the PNA–DNA duplex is hydrolyzed by nuclease S1 followed by its removal, recovery of free diffusion of the electric mediator, [Fe(CN)<SUB>6</SUB>]<SUP>3−/4−</SUP>. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c1cc11476j'> </P>

Investigation of the signaling mechanism and verification of the performance of an electrochemical real-time PCR system based on the interaction of methylene blue with DNA

Won, Byoung Yeon,Shin, Sujeong,Baek, Songyi,Jung, Ye Lim,Li, Taihua,Shin, Sung Chul,Cho, Dae-Yeon,Lee, Sang Bok,Park, Hyun Gyu Royal Society of Chemistry 2011 The Analyst Vol.136 No.8

<P>The operation of an electrochemical real-time PCR system, based on intercalative binding of methylene blue (MB) with dsDNA, has been demonstrated. PCR was performed on a fabricated electrode-patterned glass chip containing MB while recording the cathodic current peak by measuring the square wave voltammogram (SWV). The current peak signal was found to decrease with an increase in the PCR cycle number. This phenomenon was found to be mainly a consequence of the lower apparent diffusion rate of the MB-DNA complex (<I>D</I><SUB>b</SUB> = 6.82 × 10<SUP>−6</SUP> cm<SUP>2</SUP> s<SUP>−1</SUP> with 612 bp dsDNA) as compared to that of free MB (<I>D</I><SUB>f</SUB> = 5.06 × 10<SUP>−5</SUP> cm<SUP>2</SUP> s<SUP>−1</SUP>). Utilizing this signal changing mechanism, we successfully demonstrated the feasibility of an electrochemical real-time PCR system by accurately quantifying initial copy numbers of <I>Chlamydia trachomatis</I> DNA templates on a direct electrode chip. A standard calibration plot of the threshold cycle (<I>C</I><SUB>t</SUB>) value <I>versus</I> the log of the input template quantity demonstrated reliable linearity and a good PCR efficiency (106%) that is comparable to that of a conventional TaqMan probe-based real time PCR. Finally, the system developed in this effort can be employed as a key technology for the achievement of point-of-care genetic diagnosis based on the electrochemical real-time PCR.</P> <P>Graphic Abstract</P><P>The signaling mechanism of methylene blue-based electrochemical real-time PCR was scientifically investigated with verification of the analytical performances of the system. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c0an00695e'> </P>

Electrochemical Detection of DNA Mutation with PNA-Modified Electrode using S1 Nuclease

Sujeong Shin,Byoung Yeon Won,Sung Chul Shin,Hyun Gyu Park 한국생물공학회 2008 한국생물공학회 학술대회 Vol.2008 No.10

Detoxification of Tebupirimfos in Soil of Korean Ginseng Field by Human Recombinant Glutathione Stransferase M2-2

Hyesoo Jeong,Sujeong Park,Mi-yeon Kim,Minsun Chang 한국식품영양과학회 2018 한국식품영양과학회 학술대회발표집 Vol.2018 No.10

소프트웨어 불법 복제를 방지하기 위한 C 코드 난독화 도구

강서연(Seo Yeon Kang),김유민(Yumin Kim),이수정(Sujeong Lee),조은선(Eun-Sun Cho) 한국정보과학회 2020 한국정보과학회 학술발표논문집 Vol.2020 No.7

Production of Pepper Mild Mottle Virus Free Tobacco Plants by Sieving Technique

Wook Kim,Youngsun Kwon,Sujeong Lee,Ki Hyun Ryu,Ju Yeon Yoon 한국원예학회 2010 한국원예학회 학술발표요지 Vol.2010 No.10

일회용 생리대 용출액의 자외선 흡광도 시험

오혜연(Oh, Hye-yeon),고수정(Ko, Sujeong),박상수(Park, Sangsoo) 표준인증안전학회 2020 표준인증안전학회지 Vol.10 No.1

Disposable sanitary pads have been routinely used as a necessity by women for decades. However, the pads contain organic compounds in their raw materials, and they can penetrate the human body through contact with skin. Warnings on the risks of organic compounds in disposable sanitary pads are constantly being raised, but studies to reduce the risks and improve the safety of the sanitary pads are still insufficient. In this study, ten commercially available disposable sanitary pads were divided into a cotton absorbent group and a mixed absorbent group that has superabsorbent polymer beads, in addition to cotton fibers, as waterabsorbent. The products were immersed in a saline solution for 3 and 6 hrs at 37 C, and the extracted solution was measured for its absorbance at 250nm. In all 10 disposable sanitary pads, the absorbance at 250nm exceeded 0.1, the reference value of the absorbance for intrauterine contraceptive device. Comparing the UV absorbances of the extract at 250nm between the cotton absorbent group and the mixed absorbent group including the superabsorbent polymer beads, the mixed absorbent group exhibited an average of about two times higher absorbance. This means that the potential human risk of mixed absorbent pads containing superabsorbent polymer beads is about twice as large. However, the coefficient of variation of UV absorbance value among products in the same absorbent group was very large, and there was a product which showed lower UV absorbance in the mixed absorbent group than a product in the pure cotton absorbent group. These results indicate that quality control of raw materials of a sanitary pad is very important for improving the safety of a sanitary pad. 여성의 생리용품인 일회용 생리대는 현대 여성들에게 필수불가결한 제품으로 수십여년간 사용되어 왔으나, 그 원료에 함유된 유기화합물이 착용 시 접촉하는 피부를 통하여 인체에 침투할 가능성이 있다. 일회용 생리대가 함유하는 유기화합물의 위해가능성에 대한 사회적 경고가 끊임없이 제기되고 있지만, 아직까지 그 위해 가능성을 감소시키고 안전성을 향상시키기 위한 연구는 매우 부족한 편이다. 본 연구에서는 시중에서 구입가능한 일회용 생리대 10종을 순면 흡수체 제품군과 초고흡수성 고분자 비드를 포함하는 혼합 흡수체 제품군으로 구분하고, 흡수체가 함유하는 용출가능한 유기화합물의 양을 250nm 자외선 흡광도를 측정하여 비교 연구하였다. 용출액의 자외선 흡광도를 측정한 일회용 생리대 10종 모두에서 자외선 흡광도가 자궁내 피임기구의 기준규격인 250nm 기준치인 0.1을 넘게 측정되었다. 순면 흡수체 제품군과 초고흡수성 고분자 비드를 포함하는 혼합 흡수체 제품군간에 용출액의 자외선 흡광도를 비교한 결과 혼합 흡수체 제품이 평균적으로 약 2배 더 큰 흡광도를 나타냈다. 이는 초고흡수성 고분자 비드를 포함한 혼합 흡수체 제품의 잠재적 인체 위해성이 약 두 배 정도 큰 것을 의미한다. 그러나 동일 흡수체 그룹에서 제품간에 자외선 흡광도 값의 변동계수가 매우 크며, 혼합 흡수체 제품 중에서도 순면 흡수체 제품에 비하여 낮은 자외선 흡광도를 보이는 제품이 있는데 이는 제품의 생산 과정에서 원료의 품질관리가 일회용 생리대 제품의 안전성 향상에 매우 중요함을 시사한다. 그러나 순면 흡수체 제품군과 초고흡수성 고분자 비드를 포함하는 혼합 흡수체 제품군 모두 자외선 흡광도의 변동계수가 큰데 이는 같은 제품군내에서 샘플간의 250nm 흡광도 값의 차이가 큼을 뜻하며 일회용 생리대의 원료인 순면과 고분자 비드의 품질관리가 필요하다는 것을 의미한다. 자외선 흡광 스펙트럼 측정법은 생산현장에서도 쉽게 적용할 수 있는 경제적인 품질관리 방법이다. 인체와 수분 포화 상태에서 생리대에서 용출되어 인체와 접촉하는 화학물질의 위험을 최소화하기 위하여 의약외품의 기준 및 시험방법 중 일회용 생리대 부분에 자외선 흡광 스펙트럼 시험법을 포함하도록 개정할 것을 촉구한다.

Depletion of BubR1 promotes premature centrosomal localization of cyclin B1 and accelerates mitotic entry.

Park, Sun-Yi,Kim, Sujeong,Cho, Hyeseon,Kwon, Soon-Hwan,Chae, Sunyoung,Kang, Dongmin,Seong, Yeon-Sun,Cho, Hyeseong Landes Bioscience 2009 Cell cycle Vol.8 No.11

<P>The role of BubR1 has been established mainly in mitosis as an essential mitotic checkpoint protein although it is expressed throughout the cell cycle. To explore a possible role of BubR1 in regulating the G(2) phase of cell cycle, we have employed siRNA-mediated hBubR1 knockdown in HeLa cells. Here, we demonstrate that reducing BubR1 levels during the G(2) phase causes accelerated mitotic entry. As expected, BubR1 depletion leads to degradation of cyclin B(1) in the G(2) phase. Intriguingly, cyclin B(1) is prematurely targeted to centrosomes appearing at early G(2) phase in BubR1-depleted cells despite its low levels. This is in contrast to control cells where cyclin B(1) appears at the centrosomes in early prophase based on cell cycle-specific localization of CENP-F. Furthermore, cyclin B/Cdk1 kinase activity in early G(2) is aberrantly high in BubR1-depleted cells. Together, our results indicate that hBubR1 depletion triggers premature centrosomal localization of cyclin B(1) probably leading to premature mitotic entry. This study is the first to suggest a role of hBubR1 in controlling centrosome targeting of cyclin B(1) and timing of mitotic entry.</P>

Electrochemical Biosensing Platform using Magnetic Nanoparticles and Enzyme Immobilized in Mesocellular Carbon Foam

Moon Il KIM,Byoung Yeon WON,Sujeong SHIN,Hyun Gyu PARK,Youngjin YE,Jinwoo LEE 한국생물공학회 2010 한국생물공학회 학술대회 Vol.2010 No.4

대학 온라인 수업에서의 유학생 만족도와 향후 개선 방안: P대학 사례를 중심으로

안한나(Ahn, Hanna),김주연(Joo-Yeon Kim),김훈(Kim, Hoon),박명수(Park, Myeongsu),이수정(Lee, Sujeong) 학습자중심교과교육학회 2021 학습자중심교과교육연구 Vol.21 No.3

본 연구는 최근 국내 대학에서 전면적으로 실시된 온라인 수업에서 학문 목적 유학생들의 수업 만족도를 향상시키고 수업에 대한 질적 제고를 위해 유학생들의 경험을 공유하고 문제점을 진단할 필요가 있다는 인식에서 출발하였다. 이를 위해 학문 목적 유학생 80명을 대상으로 온라인 수업 환경 요인, 교수자 요인, 학습자 요인에 대한 설문조사를 실시하여 효과적인 학습 개선 방안을 찾고자 하였다. 연구 결과를 요약하면 다음과 같다. 첫째, 온라인 수업의 환경 요인에 따른 만족도에는 큰 차이가 없었으나 교수자와의 상호작용과 수업의 집중도에서 동시적 원격교육방식이 비동시적 원격교육방식보다 다소 높게 나타났다. 둘째, 교수자 요인에서는 학습자 수준에 맞는 교수가 다소 높게 나타났고 학습자와의 친밀감이 상대적으로 낮게 나타났다. 셋째, 학습자 요인에서는 참여도가 가장 높게 나타났으며 동기, 흥미, 만족도에 비해 자신감이 비교적 낮게 나타났다. 따라서 향후 학문 목적 유학생들을 위한 온라인 수업에서는 학습관리시스템(LMS)을 번역하여 제공하고 정보 소양 교육을 병행하며 유학생들의 자신감을 향상시키기 위해 자기주도학습 방안을 고안하여야 한다. 그중 에서도 비동시적 원격교육 방식과 오프라인 수업을 결합한 블랜디드 러닝(Blended Learning) 방식의 적극적 사용을 통해 학습효과를 극대화할 필요가 있다. The purpose of this study was to start with the acknowledgement that it is necessary to share the experiences of international students and diagnose problems in the recent online classes provided by domestic universities, in order to improve class satisfaction and the quality of online education. For this study, we questioned 80 international students for academic purposes and conducted online surveys on “environmental,” “instructor,” and “learner” factors to find effective ways to improve learning. The results show no significant difference in satisfaction, according to the environmental factors of online classes; however, the synchronous distance learning method scored somewhat higher than the asynchronous one regarding the interaction with the instructor and the concentration level of the class. Related to instructor factors, the teaching provided was of a slightly higher level than the students’, and the degree of closeness with learners was relatively low. Furthermore, regarding learner factors, participation registered the highest value, while confidence was relatively low compared to motivation, interest, and satisfaction. Therefore, in the future, online lessons for academic purposes should be provided by translating the Learning Management System (LMS). In addition, it will be necessary to devise a self-directed learning plan to improve international students’ confidence, in parallel with improving information quality education. Among them, it is worth considering maximizing the learning effect through active use of the blended learning method, which combines non-simultaneous remote education method and offline classes.