Quercetin: Further Investigation of its Antinociceptive Properties and Mechanisms of Action

Filho, Arnaldo Willain,Filho, Valdir Cechinel,Olinger, Leonardo,Souza, Marcia Maria de 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.6

The antinociceptive action of quercetin, a common bioactive flavonoid present in many medicinal plants, was assessed in different models of chemical and thermal nociception in mice. Quercetin (10-60 mg/kg, i.p. or 100-500 mg/kg, p.o.) dose-dependently inhibited nociceptive behavior in the acetic acid-induced pain test. Moreover, quercetin (10-60 mg/kg, i.p.) inhibited both phases of formalin-induced pain, with $ID_{50}$ values of 374.1 (68.0-402.0) mmol/kg and 103.0 (45.0-201.0) mmol/kg, for the neurogenic and inflammatory phases, respectively. Quercetin (10-60 mg/kg) also inhibited the nociception induced by glutamate and capsaicin by 68.2% and 75.5%, respectively. Its analgesic action was significantly reversed by p-chlorophenylalanine methyl ester, katanserin, methysergide, a $GABA_A$ antagonist (bicuculline), or a $GABA_B$ antagonists (baclofen). Its action was also modulated by tachykinins, but was not affected by adrenal-gland hormones. Furthermore, the antinociceptive effects did not result from muscle-relaxant or sedative action. Together, these results indicate that quercetin produces dose-related anti-nociception in several models of chemical pain, through mechanisms that involve interaction with L-arginine-nitric oxide, serotonin, and GABAergic systems. These results confirm and extend other investigations on the analgesic effect of quercetin and its mechanisms of action.

Dynamics of hospitalizations and in-hospital deaths from COVID-19 in northeast Brazil: a retrospective analysis based on the circulation of SARS-CoV-2 variants and vaccination coverage

Martins-Filho Paulo Ricardo,de Souza Araújo Adriano Antunes,Quintans-Júnior Lucindo José,Soares Bárbara dos Santos,Barboza Waneska de Souza,Cavalcante Taise Ferreira,Santos Victor Santana 한국역학회 2022 Epidemiology and Health Vol.44 No.-

This study investigated the dynamics of hospitalizations and in-hospital deaths from coronavirus disease 2019 (COVID-19) throughout the pandemic in northeast Brazil, the Brazilian region with the worst socioeconomic indicators. In total, 141,445 cases, 8,213 hospital admissions, and 1,644 in-hospital deaths from COVID-19 were registered from March 14, 2020 to February 5, 2022. The overall rates of hospitalization and in-hospital deaths were 5.8% and 20.0%, respectively. The hospitalization and death rates significantly decreased over time, which may have been related to progress in vaccination. During the spread of the Gamma variant (January to June 2021), most hospitalized individuals were young adults, and approximately 40% of deaths occurred in this age group. During the predominance of Delta (July to December 2021), over 75% of deaths occurred among the elderly and unvaccinated or partially vaccinated individuals. This rate decreased to 42.3% during the transmission of the Omicron variant (January to February 2022), during which 34.6% of deaths were recorded among fully vaccinated individuals (2 doses) and 23.1% among those who received full vaccination and a booster. The Omicron-driven third wave was associated with a rise in the proportion of deaths among vaccinated individuals, especially among those who had not received a booster dose.

Push-out bond strength of a self-adhesive resin cement used as endodontic sealer

Gurgel-Filho, Eduardo Diogo,Lima, Felipe Coelho,Saboia, Vicente De Paula Aragao,Coutinho-Filho, Tauby De Souza,Neves, Aline De Almeida,da Silva, Emmanuel Joao Nogueira Leal The Korean Academy of Conservative Dentistry 2014 Restorative Dentistry & Endodontics Vol.39 No.4

Objectives: The aim of the present study was to investigate the bond strength of RelyX Unicem (3M) to root canal dentin when used as an endodontic sealer. Materials and Methods: Samples of 24 single-rooted teeth were prepared with Gates Glidden drills and K3 files. After that, the roots were randomly assigned to three experimental groups (n = 8) according to the filling material, (1) AH Plus (Dentsply De Trey GmbH)/Gutta-Percha cone; (2) Epiphany SE (Pentron)/Resilon cone; (3) RelyX Unicem/Gutta-Percha cone. All roots were filled using a single cone technique associated to vertical condensation. After the filling procedures, each tooth was prepared for a push-out bond strenght test by cutting 1 mm-thick root slices. Loading was performed on a universal testing machine at a speed of 0.5 mm/min. One-way analysis of variance and Tukey test for multiple comparisons were used to compare the results among the experimental groups. Results: Epiphany SE/Resilon showed significantly lower push-out bond strength than both AH Plus/Gutta-Percha and RelyX Unicem/Gutta-Percha (p < 0.05). There was no significant difference in bond strength between AH Plus/Gutta-Percha and RelyX Unicem/Gutta-Percha (p > 0.05). Conclusions: Under the present in vitro conditions, bond strength to root dentin promoted by RelyX Unicem was similar to AH Plus. Epiphany SE/Resilon resulted in lower bond strength values when compared to both materials.

Killing two birds with one stone: How do Plant Viruses Break Down Plant Defenses and Manipulate Cellular Processes to Replicate Themselves?

Pedro Filho Noronha Souza,Fabricio Eulálio Leite Carvalho 한국식물학회 2019 Journal of Plant Biology Vol.62 No.3

As simple organisms with a parasite nature,viruses have become masters in manipulating and subvertcellular components, including host proteins and organelles,to improve viral replication. Therefore, the understanding ofviral strategies to manipulate cell function disrupting plantdefenses and enhancing viral infection cycles is fundamentalto the production of virus-resistant plant lines. After invadingsusceptible plants, viruses create conditions that favor localand systemic infections by suppressing multiple layers ofinnate host defenses while use cellular machinery to ownbenefit. Viral interference in interlinked essential cellularfunctions results in phenotypic changes and disease symptoms,which debilitates plants favoring infection establishment. Herein in this review, the novelty it will be the discussionabout the strategies used by (+) single strand RNA viruses toaffect cellular processes and components to improve viralreplication, in parallel to overcome plant defenses, favoringdisease establishment by applying in one action using thesame viral protein to coordinate viral replication and breakingdown plant defense. This focus on plant-virus interactionwas never done before, and this knowledge has the potentialto help in the development of new strategies to produceresistant plants.

Quercetin: Further Investigation of its Antinociceptive Properties and Mechanisms of Action

Arnaldo Willain Filho,Valdir Cechinel Filho,Leonardo Olinger,Marcia Maria de Souza 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.6

The antinociceptive action of quercetin, a common bioactive flavonoid present in many medicinal plants, was assessed in different models of chemical and thermal nociception in mice. Quercetin (10-60 mg/kg, i.p. or 100-500 mg/kg, p.o.) dose-dependently inhibited nociceptive behavior in the acetic acid-induced pain test. Moreover, quercetin (10-60 mg/kg, i.p.) inhibited both phases of formalin-induced pain, with ID50 values of 374.1 (68.0-402.0) mmol/kg and 103.0 (45.0-201.0) mmol/kg, for the neurogenic and inflammatory phases, respectively. Quercetin (10-60 mg/kg) also inhibited the nociception induced by glutamate and capsaicin by 68.2% and 75.5%, respectively. Its analgesic action was significantly reversed by p-chlorophenylalanine methyl ester, katanserin, methysergide, a GABAA antagonist (bicuculline), or a GABAB antagonists (baclofen). Its action was also modulated by tachykinins, but was not affected by adrenal-gland hormones. Furthermore, the antinociceptive effects did not result from muscle-relaxant or sedative action. Together, these results indicate that quercetin produces dose-related anti-nociception in several models of chemical pain, through mechanisms that involve interaction with L-arginine-nitric oxide, serotonin, and GABAergic systems. These results confirm and extend other investigations on the analgesic effect of quercetin and its mechanisms of action.

Glycerol Potentiates the Effects of Glucose in Promoting Glucose Recovery During Hypoglycemia: From Basic to Clinical Investigations and Their Therapeutic Application

Wilson Eik Filho,Bruna Juliana Wanczinski Ferrari,Marina Masetto Antunes,Patrícia Batista Travassos,Helenir Medri de Souza,Eniuce Menezes de Souza,Roberto Barbosa Bazotte 한국식품영양과학회 2021 Journal of medicinal food Vol.24 No.9

We compared the effect of oral glucose versus oral glucose combined with glycerol (glucose + glycerol) in promoting glucose recovery during hypoglycemia. These studies were carried out in two series of experiments. In the first series of experiments, 16 overnight fasted rats received an intraperitoneal injection of lispro insulin (1 IU/kg), and 25 min later, they received oral water (control), glucose (0.25 g/kg), glycerol (2.5 g/kg), or glucose (0.25 g/kg) + glycerol (2.5 g/kg). In the second series of experiments on 164 eligible type 1 diabetic (T1D) patients, 30 individuals with a history of hypoglycemia were recruited. Five volunteers did not meet the inclusion criteria and two subjects were excluded after starting the clinical investigation; 23 patients concluded the study. All patients with symptoms of hypoglycemia ingested oral glucose (15 g) or glucose (15 g) + glycerol (9.45 g). To treat hypoglycemia in T1D patients, preparations containing glucose alone or glucose + glycerol were used alternately (2 weeks/2 weeks) in a double-blind crossover scheme. Throughout the clinical research (4 weeks), glucose concentrations were assessed with a continuous glucose monitoring device and the results after the use of glucose alone or glucose + glycerol preparations were compared. Oral glucose combined with glycerol was more effective in promoting glucose recovery in comparison with glucose alone, not only in rats but also in T1D patients. Taken together, our experimental and clinical investigations reported the best performance of oral administration of glucose + glycerol in comparison with isolated glucose.

Push-out bond strength of a self-adhesive resin cement used as endodontic sealer

Eduardo Diogo Gurgel-Filho,Felipe Coelho Lima,Vicente de Paula Aragão Saboia,Tauby de Souza Coutinho-Filho,Aline de Almeida Neves,Emmanuel João Nogueira Leal da Silva 대한치과보존학회 2014 Restorative Dentistry & Endodontics Vol.39 No.4

Objectives: The aim of the present study was to investigate the bond strength of RelyX Unicem (3M) to root canal dentin when used as an endodontic sealer. Materials and Methods: Samples of 24 single-rooted teeth were prepared with Gates Glidden drills and K3 files. After that, the roots were randomly assigned to three experimental groups (n = 8) according to the filling material, (1) AH Plus (Dentsply De Trey GmbH)/ Gutta-Percha cone; (2) Epiphany SE (Pentron)/Resilon cone; (3) RelyX Unicem/Gutta- Percha cone. All roots were filled using a single cone technique associated to vertical condensation. After the filling procedures, each tooth was prepared for a push-out bond strenght test by cutting 1 mm-thick root slices. Loading was performed on a universal testing machine at a speed of 0.5 mm/min. One-way analysis of variance and Tukey test for multiple comparisons were used to compare the results among the experimental groups. Results: Epiphany SE/Resilon showed significantly lower pushout bond strength than both AH Plus/Gutta-Percha and RelyX Unicem/Gutta-Percha (p < 0.05). There was no significant difference in bond strength between AH Plus/Gutta- Percha and RelyX Unicem/Gutta-Percha (p > 0.05). Conclusions: Under the present in vitro conditions, bond strength to root dentin promoted by RelyX Unicem was similar to AH Plus. Epiphany SE/Resilon resulted in lower bond strength values when compared to both materials.

The In Vitro Genotoxic Effect of Tucuma (Astrocaryum aculeatum), an Amazonian Fruit Rich in Carotenoids

Olmiro Cezimbra de Souza Filho,Michele Rorato Sagrillo,Luiz Filipe Machado Garcia,Alencar Kolinski Machado,Francine Cadona,Euler Esteves Ribeiro,Marta Maria Medeiros Frescura Duarte,Ademir Farias More 한국식품영양과학회 2013 Journal of medicinal food Vol.16 No.11

Tucuma (Astrocaryum aculeatum) is an Amazonian fruit that presents high levels of carotenoids and other bioactive compounds such as quercetin. The extracts of tucuma peel and pulp present strong antioxidant activity which illustrate an elevated concentration that causes cytotoxic effects in human peripheral blood mononuclear cells (PBMCs). This study performed additional investigations to analyze the potential genotoxic effects of the tucuma extracts on PBMCs. The genotoxicity was evaluated by DNA fragmentation, Comet assay, and chromosomal instability G-band assays. The acute tucuma extract treatment showed genoprotective effects against DNA denaturation when compared with untreated PBMC cells. However, in the experiments with 24 and 72 h treatments to tucuma treatments, we observed low genotoxicity through a concentration of 100 lg/mL, some genotoxic effects related to intermediary concentrations (100–500 lg/mL), and more pronounced genotoxic effects on higher tucuma extract concentrations. After 24 h of treatment, the reactive oxygen species were similar among treatments and PBMC control groups. However, the caspase-1 activity related to the apoptosis and pyroptosis process increased significantly in higher tucuma concentrations. In summary, tucuma extracts, despite their higher antioxidant content and antioxidant activity, would present PBMCs genotoxic effects that are dependent on concentration and time exposition. These results need to be considered in future in vitro and in vivo studies of tucuma effects.

Economic Evaluation of Public Policies Aiming the Reduction of Greenhouse Gas Emissions in Brazil

( Joaquim Bento De Souza Ferreira Filho ),( Marcelo Theoto Rocha ) 세종대학교 경제통합연구소 (구 세종대학교 국제경제연구소) 2008 Journal of Economic Integration Vol.23 No.3

In this paper a static, inter-regional and bottom-up general equilibrium model of greenhouse gas emissions in Brazil is used to analyze the impact of different types of carbon taxes on the economy. The core database is calibrated with Brazilian economic data from 1996, while the emissions module is based on the Brazilian Initial National Communication to the United Nations Convention about Global Climate Change for the 1994 reference year. The gas module in the model comprises all known sources of greenhouse gases emissions except emissions from land use change (deforestation). The simulations comprise scenarios with carbon taxes on emissions, either on fuel use or on the activity level of industries. Results show that taxing activities is more relevant for greenhouse gases emissions reductions in Brazil than just taxing fuel use, due to the importance of activity related emissions in the Brazilian emissions matrix. Livestock is found to be one of the most important emission sectors in Brazil. Carbon tax on activities, however, generates the higher increase in food prices, with negative implications for poverty alleviation. Different carbon tax schemes would also have different regional impacts inside Brazil, with taxation on fuels harming more the Southeast and Northeast regions, and taxation on activity levels affecting negatively more the South and Center west regions.

Experimental Factorial Designs to Produce Niobium Precursor by Spray Drying Process

Souza Carlson,Bezerra Marcio B. D.,Fontes Francisco A. O.,Filho Ulisses C. O. 한국분말야금학회 2006 한국분말야금학회 학술대회논문집 Vol.2006 No.1