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Climate change effects on Asian honey bees and bee keeping
Siriwat Wongsiri 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.10
Climate change and global warming are directly effecting the population dynamics of insects of medical importance and insect pests of agricultural commodities during the last few years. The outbreak of some insect-borndiseases and decreasing yield of agricultural products are both caused and results of climate change are known everywhere in the world. Recent reports of honey bee diseases and out breaks, as well as increase in the incidence of CCD(Collapse Colonial Disease) are causing great concerns and pose big problem for our bee keepers in many countries in North America and Europe. These important infectious diseases are possible carried and propagated by bee mites primarily by Varroa mites, which have recently experienced increasing populations in USA and UK includes some European countries. Recently some Asian honey bees adapted to live in the urban areas as the example of Apis dorsata move to Mae Fah Luang Campus more than 30 colonies and even in Chulalonkorn Campus more than 10 colonies increase from few colonies in the the last few years. Apis florea have been found more than 161 colonies this year in Kanchanaburi (River Kwai province) this year(2009). The discussion of some wild honey bees migration will concentrate on research program of our bee research unit of the university in Thailand.
Boonmee Kavinseksan,Siriwat Wongsiri 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.2
The purpose of this study was to determine grooming behavior efficacy of Apis dorsata, Thai commercial (A. mellifera, Italian honey bee hybrids), and Primorsky honey bees (A. mellifera) in killing the mite species Euvarroa sinhai. Ten A. dorsata, 15 Thai commercial and 15 Primorsky colonies were used to compare efficacy in their grooming behavior related to E. sinhai. Fallen E. sinhai from A. dorsata colonies was collected using a white plastic sheet (1.4 × 2 m), which was smeared by vegetative oil and placed 1 m below each colony. The fallen E. sinhai on the plastic sheet were collected weekly until the colony was absconded. Dead E. sinhai from each colony of Thai commercial and Primorsky honey bees were collected by the use of mite collecting boards with wire screens. The boards were coated with a thin film of vegetable oil and maintained in the hives for periods of 7 days until the end of the experiment (12 months). Dead mites on the traps from A. dorsata, Thai commercial, and Primorsky colonies were retrieved by the use of a fine paint brush and examined for their injuries using a stereomicroscope at 40× magnification. Injuries obtained by the mites were classified as either injured legs only or injured both legs and body. The data on percent means of injured mites from colonies of the three bee types were analyzed using one-way ANOVA for a completely randomized design, and the means were compared using Duncan's new multiple range test. The results showed that A. dorsata workers had more efficient grooming behavior to kill E. sinhai than the Primorsky and Thai commercial bees, respectively. The percentage of injured mites in the A. dorsata debris (55.4 ± 2.2%) was significantly higher (P = 0.001, df = 37) than that of the Thai commercial (18.6 ± 1.6%) and Primorsky (41.4 ± 2.1%) debris (percent mean ± standard error), and the percentage of injured mites in the Primorsky debris was significantly higher than that of the Thai commercial debris. The percentage of mites that had severely injured both legs and body in the A. dorsata debris (42.0%) was higher than that of the Thai commercial (31.8%) and Primorsky (36.0%) debris. Mites that had injured legs only from the A. dorsata, Thai commercial, and Primorsky debris were 58.0%, 68.2%, and 64.0%, respectively.
Atsalek Rattanawannee,Chanpen Chanchao,Siriwat Wongsiri 한국응용곤충학회 2012 Journal of Asia-Pacific Entomology Vol.15 No.4
Geometric morphometry was used to characterize 73 Apis dorsata colonies collected from 31 different localities in five major geographic regions of mainland Thailand. We measured 19 easily identified landmarks from the digitized images of the right forewing of 10 worker bees from each colony (730 bees in total);thus, avoiding the confounding variation from haploid or diploid males. After plotting the factor scores,A. dorsata from (mainland) Thailand were found to belong to a single group, which was further supported by a hierarchical cluster analysis-generated dendrogram. Multivariate analysis of variance (MANOVA, α=0.05) demonstrated no significant differences among the five geographic groups of A. dorsata in Thailand,producing a low degree of accuracy (31.2%) in the identification of the geographic region from which any individual bee originated. Additionally, when the bee samples were classified into two groups, those north and south of the Isthmus of Kra were not significantly different (MANOVA, α=0.05), and a low rate of correct classification in a cross-validation test (65% correct) was found. Therefore, this geometric morphometric based analysis of worker bee wing venation pattern suggests that A. dorsata populations in mainland Thailand are panmictic.
Srisuparbh, Duangporn,Klinbunga, Sirawut,Wongsiri, Siriwat,Sittipraneed, Siriporn Korean Society for Biochemistry and Molecular Biol 2003 Journal of biochemistry and molecular biology Vol.36 No.6
An expressed sequence tag (EST) library was established from the hypopharyngeal glands of Apis cerana. Sixty-six recombinant clones, possessing inserts >500 bp, were randomly selected and unidirectional sequenced. Forty-two of these (63.6%) were identified as homologues of Major Royal Jelly Proteins families 1, 2, 3, and 4 of A. mellifera (AmMRJP) for which MRJP1 was the most abundant family. The open-reading frame of the MRJP1 homologue (AcMRJP1) was 1299 nucleotides that encoded 433 deduced amino acids with three predicted N-linked glycosylation sites. The AcMRJP1 sequence showed 93% and 90% homologies with nucleotide and deduced amino acid sequences of AmMRJP1, respectively. Two complete transcripts of apisimin, and one and two partial transcripts of $\alpha$-glucosidase and glucose oxidase, were also isolated. In addition, the royal jelly proteins of A. cerana were purified and characterized using Q-Sepharose and Sephadex G-200 column chromatography. The native forms of protein peaks A1, A2, B1, and C1 were 115, 55, 50, and 300 kDa, respectively. SDS-PAGE analysis indicated that A1 and C1 were dimeric and oligomeric forms of the 80 kDa and 50 kDa subunits, respectively. The ratio of the total protein quantities of A1 : A2 : B1 : C1 were 2.52 : 4.72 : 1 : 12.21. Further characterization of each protein, using N-terminal and internal peptide sequencing, revealed that the respective proteins were homologues of MRJP3, MRJP2, MRJP1, and MRJP1 of A. mellifera.
( Duangporn Srisuparbh ),( Sirawut Klinbunga ),( Siriwat Wongsiri ),( Siriporn Sittipraneed ) 생화학분자생물학회 2003 BMB Reports Vol.36 No.6
An expressed sequence tag (EST) library was established from the hypopharyngeal glands of Apis cerana. Sixty-six recombinant clones, possessing inserts >500 bp, were randomly selected and unidirectional sequenced. Fprty-two of these (63.6%) were identified as homologues of Major Royal Jelly Proteins families 1, 2, 3, and 4 of A. mellifera (AmMRJP) for which MRJP1 was the most abundant family. The open-reading frame of the MRJP1 homologue (AcMRJP1) was 1299 nucleotides that encoded 433 deduced amino acids with three predicted N-linked glycosylation sites. The AcMRJP1sequence showed 93 % and 90% homologies with nucleotide and deduced amino acid sequences of AmMRJP1, respectively. Two complete transcripts of apisimin, and one and two partial transcripts of α-glucosidase and glucose oxidase, were also isolated. In addition, the royal jelly proteins of A. cerana were purified and characterized using Q-Sepharose and Sephadex G-200 column chromatography. The native forms of protein peaks Al, A2, B1, and C1 were 115, 55, 50, and 300kDa, respectively. SDS-PAGE analysis indicated that A1 and C1 were dimeric and oligomeric forms of the 80 kDa and 50 kDa subunits, respectively. The ratio of the total protein quantities of A1 :A2 :B1 : C1 were 2.52 : 4.72 : 1 : 12.21. Further characterization of each protein, using N-terminal and internal peptide sequencing, revealed that the respective proteins were homologues of MRJP3, MRJP2, MRJP1, and MRJPl of A. mellifera.