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- 저자 : Shu-Lung Yang (검색결과 2 건)
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Shu-Lung Yang 아시아경찰학회 2003 아시아경찰학회 국제학술회의 Vol.2003 No.-
The purpose of this study is to investigate how police detectives prioritize criminal cases, and what factors they consider in solving any given case in Taiwan. Factors related to the decision-making process of investigation initiation include: the seriousness of the offense, order from higher-ranking officers, media sensibility, etc. Convincing evidence, a suspect's confession, qualified/trained detectives, and cooperation among staff are identified as possible solvability factors in the study. The criminal cases in the study are limited to homicide, sexual assault and theft. The study sent questionnaires to 26 Criminal Investigation Divisions of each individual county-level police department in Taiwan, the Republic of China (ROC) between July 1 and August 15, 2003. In all, 1,072 police detectives were surveyed. Homicide, kidnapping, robbery, weapon use, and sexual assault were the most important criminal cases that the detectives initiated response to. On the other hand, cases of vandalism, anti-intellectual property, gambling, simple assault, and fraud tend to be suspended by the detectives, and will not dealt with actively. The critical factors influencing the decision to initiate an investigation are ranked accordingly as: the social harm, order from higher-ranking officers, the extent of damage, rewards and punishments, and media sensibility. For homicide case cleared, the important/critical factors are in order of importance: the weight of collected evidence, the presence of an eye-witness, the quality and experience of detectives, qualified forensic science personnel, and the a well-constructed criminal database. For sexual offense cases cleared, the important factors in order of importance are: the weight of collected evidence, the completeness of the DNA database, the victim's report, the relationship between offender and victim, the experience and quality of detectives. For thefts cleared, the important/critical factors are as follows: the weight of collected evidence, control of the fence, the suspects continued use of the stolen goods, the experience and quality of detectives, qualified forensic science personnel. To improve the quality of crime detection, the detectives' viewpoints argue that efforts should be made to promote the effectiveness of forensic science, provide advanced equipment for detection, reinforce legal training, increase official pay, and create specialized crime detection divisions.
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( Chia-yen Dai ),( Shu-chi Wang ),( Meng-hsuan Hsieh ),( Cheng-fu Yang ),( Ching-i Huang ),( Chung-feng Huang ),( Ming-lun Yeh ),( Jee-fu Huang ),( Wang-long Chung ),( Ming-lung Yu ) 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1
Aims: The different hepatitis C virus (HCV) replication has been reported among individual hepatocytes in chronic HCV infection by identifying hepatocytes with different HCV RNA levels. We have previously established a fluorescence-activated cell sorting (FACS) protocol to study the effects of different intracellular viral loads in HCV-infected cells. The present study aimed to further study the gene expression on different hepatocellular carcinoma (HCC) cells with different HCV viral load. Methods: The JFH1-EYFP viral florescence intensity was used to sort the high and low viral load cells after 5 days infection in vitro which has been shown in our previous study that infected cells efficiently and accurately discriminated between high- and low-viral load cell populations. The next generation sequence-RNA sequence was used to clarify the mRNA and miRNA gene network between HCV-high and HCV-low infected cells of the HCC cell line. Venn diagram summarizing the probe sets that were differentially expressingbetween the Huh7.5.1 versus each differential viral load cell population and miRDB and miRTar databases were used to predict HVL and LVL/S2 unique miRNA target genes. Results: By analyzing the NGS dataset and miRNA microarray dataset, of the significant transcripts, three miRNA were unique for the LVL/S2 cells and nine miRNA unique for the HVL. Twenty-three miRNA were common for all 3 viral load groups. We verified them by q-PCR and data confirmed the array data expression level. We found that high viral loads were associated with cell inflammation- and cell death-associated pathway; and the low viral loads were associated many stress response- and cell adhesion molecular (CAMs)-related genes. Conclusions: With the established cell sorting protocol, we have demonstrated that different gene network between HCV-high and HCV-low infected cells in JFH1-EYFP infectious cells exists. Our results may provide a boarder gene regulation map between high and low viral load cell populations.
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