成熟赤血球의 Evans´blue 吸收에 미치는 二價金屬이온의 影響에 關한 硏究

姜信英,曺龍鎬 慶熙大學校 1984 論文集 Vol.13 No.-

Non-nucleated mature erythrocytes of human blood were stored at 4℃ in darkness for 4 hours. Upon the storage erythrocytes were separated by the fractional centrifugal method. The effect of divalent metallic ions on the percent of dye uptake rate of Evans' blue as a neutral salt of a organic dye in the presence of 580mM glucose was observed and the following results were obtained. 1. The dye uptake rate was found to be kinetically proportional in function of incubation period in the stored eryrocytes for 4 hours with the dye uptake of 42.2 percent for 120 minutes incubation. 2. The statistical analysis of this effect of the relationship between the incubation period and the percent dye uptake rate was found to be very highly significant (p<0.001). 3. The effect of magnesium ion (5 mM) on the percent dye uptake rate in function of incubation times was kinetically increased in human matured erythrocytes when compared to that of control group. At the end of 40 minutes of incubation the human matured erythrocytes treated with magnesium ion ( 5 mM) increased about 1.5 times more of 30.5 percent dye uptake than the control group of 20.3 percent dye uptake. 4. The effect of magnesium ion was slightly accelerated with the addition of 0.01 mM magnesium ion; between 0.05 mM and 10 mM of magnesium ions the influence of this divalent metallic ion was greatest. 5. The increase in the concentration of copper ion inhibited proportionally and remarkablly the percent dye uptake rate by human matured erythrocytes. Fifty percent inhibitory concentration (1_50) of copper ion on the percent dye uptake rate by human matured erythrocytes was found to be 10.07 micromoles. 6. Also, the effect of ferrous ion (5 micromoles) on the percent dye uptake rate is human matured erythrocytes for 40 minutes incubation was found to be 21.4 percent inhibition and it inhibited that the effect of hematin (5 micormoles) on the percent dye uptake rate o Evans' blue by 65.6 percent points for 40 minutes incubation. 7. No effect was exerted on the percent dye uptake rate in human matured erythrocytes during the incubation periods by the addition of calciumion (5 mM) and manganese ion (tmicromoles). 8. The addition of lithium ion at the concentration of 5 micormoles to reactant mixture was effectively increased on the percent dye uptake rate of Evans' blue for a incubation of 40 minutes when compared to that control group (20.3%) to 43.9 percent. In view of the above findings it is clear that the effect of magnesium ion and lithium ion, as divalent metallic ions, on the Evans' blue absorbed into non-nucleated erythrocytes is considered to accelerate this enhancing effect while it does not accelerate that of hematin and copper ion. However, the effects of calcium ion and manganese ion was ineffective on the percent dye uptake of Evans' blue by human matured erythrocytes.

米麥混食이 Cholesterol 및 無機質代射에 미치는 影響

姜信英,孫延瑗,柳總根 고려대학교 의과대학 1986 고려대 의대 잡지 Vol.23 No.1

This study was devised to investigate the effect of dietary fiber of barley on cholesterol metabolism and absorption of mineral on feeding either rice diets or barley mixed diets. Wistar albino rats were administered starch, rice, rice containing balrey by 10%, 30%, and 60% and barley diets for 2, 4, and 6 weeks. 1. Serum cholesterol level was higher in the rice diet group than in the barley diet group. In the meantime, fecal steroid content was highest in the barley diet group and decreased as the proportion of barley in diets became lower. 2. Correlation coefficient between the increase of fecal n-eight and the increase of fecal steroid content was 0. 988(p<0. 001). 3. Fecal Fe and Ca content was net affected by the extent of barley and rice in diets. But fecal Ka and K content was high in the barley diet group. 4. Fecal weight and fecal moisture content was highest in the barley diet group, and became lower in the diet group where barley was added in smaller proportion. The increase of fecal weight was significantly related with the increase of fecal water content(r=0.918, p<0.001). 5. Serum lipid level was lower in the diet group containing more barley than in the rice diet group. In contrast, focal lipid content was highest in the barley diet group, and decreased in proportion to the decrease of barley addition in diets. 6. Accumulation of adipose tissue was observed In the rice diet group, 2nd the more barley mixed in diets, the lower it was. 7. Length of small intestine was longer in the barley diet group than in the rice diet group.

샷 분류에 의한 교육용 비디오 자료에서의 대표화면 추출

유신(Sheen Lew),조선길(Sungil Cho),신희정(HeeJung Sheen),양춘석(Yang Chunsuck),이완주(WanJoo Lee),이병래(ByeongRae Lee),김용규(Yongkyu Kim),강현철(Hyunchul Kang) 한국통신학회 1999 한국통신학회 학술대회논문집 Vol.1999 No.7

교육용 비디오 자료에서의 자막 특성을 이용한 대표화면추출 및 자막화면추출에 관한 연구

유신(Sheen Lew),신희정(HeeJung Sheen),이완주(WanJoo Lee),이병래(Yongkyu Kim),김용규(ByeongRae Lee),강현철(Hyunchul Kang) 한국정보과학회 1999 한국정보과학회 학술발표논문집 Vol.26 No.1B

본 연구에서는 자막특성을 이용하여 대표 프레임 추출과 문자 인식을 위한 문자 프레임 추출을 수행함과 동시에 장면전환을 검출할 수 있는 시스템을 구현하였다. 이는 기존의 방식인 장면전환 검출을 통해 비디오자료를 샷(shot)단위로 구조화하고 단순대표 프레임으로 각 샷의 내용을 요약하는 방식과는 달리, 비디오 스트림의 내용에 기반하여, 중요도가 상대적으로 높은 프레임을 대표 프레임으로 추출, 대표 프레임의 의미적(semantic)효용성을 보장한다. 이러한 기법은 교육용 비디오자료 특성에 기반하여 적용되었으며, 본 논문에서 제안한 시스템은 수행시간과 정확성면에서 뛰어난 특성을 보였다.

The impact of size on tissue distribution and elimination by single intravenous injection of silica nanoparticles

Yhun Yhang, Sheen,Minjung, Cho,Wan-Seob, Cho,Mina, Choi,Sueng Jun, Kim,Beam Seak, Han,Sheen Hee, Kim,Hyoung Oak, Kim,Ja Young, Jeong 이화여자대학교 약학연구소 2010 藥學硏究論文集 Vol.- No.20

Many approaches for the application of nano-sized particles to the human body as nanotechnology have been recently developed. The size of nanoparticles is related to their useful character and also plays a key role in toxicity. Since this surface area can interact with biological components of cells, nanoparticles can be more reactive in than larger particles. In the present study, a fluorescence dye-labeled 50, 100 and 200 nm-sized silica particle suspension was intravenously injected into mice to identify the toxicity, tissue distribution and excretion of silica nanoparticles in vivo. Incidence and severity of inflammatory response was transiently increased with injection of 200 and 100 nm silica nanoparticles within 12h. But there was no significant response related to injection of 50 nm particles. The silica particles of 50, 100 and 200 nm were cleared via urine and bile. The 50 nm silica anoparticles cleared to urine and bile than 100 nm and particles of 200 nm existed at lower concentration than other two smaller particles in urine and feces. Silica nanoparticles were trapped by macrophages in the spleen and liver and remained there until 4 weeks after the single injection.

미용서비스 종사자의 직무스트레스와 직무소진의 관계에서 자아탄력성의 매개효과

신영식(Young Seek Sheen),권도희(Do Hee Kwon),오경희(Kyung Hee Oh),신라미(La Mee Sheen) 한국인체미용예술학회 2017 한국인체미용예술학회지 Vol.18 No.4

This study aimed to figure out correlations among job stress, ego resilience and job burn out in beauty salon workers. For this, a questionnaire survey was administered to 300 beauty salon workers in Busan and the capital region from May 2nd to 31st, 2014. Except for 44 poorly answered ones, 256 copies were used for a final analysis. For analysis of the data, the analysis was conducted to analyze the frequency analysis and the validity of the measurement of the measurement participants’ characteristics. In addition, a correlation analysis was conducted to verify the validity of the variables and the identification validity of the variables, and conducted a regression analysis to verify the suitability and validity of the research model. The study results found the following: while a positive correlation was found between job stress and job burn out, a negative correlation was observed between ego resilience and job burn out. In other words, job stress has a negative effect on beauty salon workers’ life as well as their working environment. In contrast, ego resilience reduces job burn out by helping staff build the ability to handle problems in a positive manner.

A study of fracture loads and fracture characteristics of teeth

Sheen, Chang-Yong,Dong, Jin-Keun,Brantley, William Arthur,Han, David Seungho The Korean Academy of Prosthodonitics 2019 The Journal of Advanced Prosthodontics Vol.11 No.3

PURPOSE. The purpose of this in vitro study was to investigate the fracture loads and modes of failure for the full range of natural teeth under simulated occlusal loading. MATERIALS AND METHODS. One hundred and forty natural teeth were taken from mandibles and maxillas of patients. There were 14 groups of teeth with 10 teeth in each group (5 males and 5 females). Each specimen was embedded in resin and mounted on a positioning jig, with the long axis of the tooth at an inclined angle of 30 degrees. A universal testing machine was used to measure the compression load at which fracture of the tooth specimen occurred; loads were applied on the incisal edge and/or functional cusp. RESULTS. The mean fracture load for the mandibular first premolar was the highest (2002 N) of all the types of teeth, while the mean fracture load for the maxillary first premolar was the lowest (525 N). Mean fracture loads for the mandibular and maxillary incisors, and the first and second maxillary premolars, had significantly lower values compared to the other types of teeth. The mean fracture load for the teeth from males was significantly greater than that for the teeth from females. There was an inverse relationship between age and mean fracture load, in which older teeth had lower fracture loads compared to younger teeth. CONCLUSION. The mean fracture loads for natural teeth were significantly different, with dependence on tooth position and the sex and age of the individual.

Dimension Reduction of Solid Models by Mid-Surface Generation

Sheen, Dong-Pyoung,Son, Tae-Geun,Ryu, Cheol-Ho,Lee, Sang-Hun,Lee, Kun-Woo Society for Computational Design and Engineering 2007 International Journal of CAD/CAM Vol.7 No.1

Recently, feature-based solid modeling systems have been widely used in product design. However, for engineering analysis of a product model, an ed CAD model composed of mid-surfaces is desirable for conditions in which the ed model does not affect analysis result seriously. To meet this requirement, a variety of solid ion methods such as MAT (medial axis transformation) have been proposed to provide an ed CAE model from a solid design model. The algorithm of the MAT approach can be applied to any complicated solid model. However, additional work to trim and extend some parts of the result is required to obtain a practically useful CAE model because the inscribed sphere used in the MAT method generates insufficient surfaces with branches. On the other hand, the mid-surface ion approach supports a practical method for generating a two-dimensional ed model, even though it has difficulties in creating a mid-surface from some complicated parts. In this paper, we propose a dimension reduction approach on solid models based on the midsurface abstraction approach. This approach simplifies the solid model by abbreviating or removing trivial features first such as the fillet, mounting, or protrusion. The geometry of each face is replaced with mid-patches from the simplified model, and then unnecessary topological entities are deleted to generate a clean ed model. Also, additional work, such as extending and stitching mid-patches, completes the generation of a mid-surface model from the patches.

Effect of 3-Methylcholanthrene on Rat Uterus : Uterine Growth and Mechanism of Action of 3-Methylcholanthrene

Sheen, Yhun Y.,Kim, Sun S.,Yun, Hea C. 이화여자대학교 생명과학연구소 1993 생명과학연구논문집 Vol.4 No.-

This stydy has been undertaken to examine the effect of 3-methylcholanthrene (3MC) on rat uterine growth and to undertstand the mechanism of action of 3MC in rat uterus. After diethylstilbesterol(DES) or tamoxifen(TAM) or 3MC or DES plus TAM or DES plus 3MC was administered into immature female rats, uterine weight of each group was measured. DES treatment resulted in 4-fold increase in uterine weight over com oil-treated uteri. 3MC treatment had no effect on uterine weight but, DES stimulated uterine weight was inhibited by 3MC concomitant treatment. While TAM alone treatment showed slight increase in uterine weight, inhibited uterine growth stimulated by DES when it was administrated with DES concomitantly. Affinity of estradiol for estrogen receptor in the rat uterus was determined via direct binding assay with [^3H]estradiol and the relative binding affinities of 3MC and TAM were estimated by competetion assay. Estradiol tumed out to have high affinity for rat uterine estrogen receptor (Kd=0.4nM). The relative binding affinities of TAM and 3MC were 1% and 4.7% that of DES for rat uterine estrogen receptor, respectively. 3MC was shown to have similar affinity for rat uterine estrogen receptor to that of TAM. Effects of DES, 3MC and TAM administration in vivo on rat uterine estrogen receptor level were examined. It was confirmed that the estrogen, DES and antiestrogen, TAM decreased estrogen receptor levels from rat uterus and also 3MC decreased rat uterine estrogen receptor level when rats were treated with DES, TAM and 3MC in vivo. Data indicates that 3MC acts as an antiestrogen mediated through estrogen receptor system.

IN -1130 : Distribution and Metabolism in vivo

SHEEN Yhun Yhong,KIM Dae-Kee,MIN Kyung Nan,KIM Yong Kook,JEE Jung In,KIM Yeo Woon 大韓藥學會 2006 大韓藥學會 總會 및 學術大會 Vol.2006 No.2