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RISS 인기검색어
- 검색키워드
- 저자 : Seung Joo Go ) (검색결과 163 건)
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Ribosomal DNA의 ITS영역 염기서열 분석에 의한 기주특이적 독소 생성 Alternaria spp.의 계통분류학적 유연관계
高昇柱,洪承範,姜熙玩,劉勝憲,柳震彰 충남대학교 생물공학연구소 1998 생물공학연구지 Vol.6 No.-
기주특이적 독소 생성 Alternaria spp.와 비병원성 A. alternata의 계통분류학적 유연관계를 규명하기 위하여 기주특이적 독소 생성 Alternaria 6균, 비병원성인 A. alternata 그리고 비교균으로서 소형포자를 형성하는 A. brassicicola, 대형포자를 형성하는 A. solani의 ribosmal DNA의 5.8S를 포함한 ITS영역의 염기 서열을 분석하였던 바 다음과 같은 결과를 얻었다. 1. Alternaria spp.의 rDNA ITS영역의 염기서열 크기는 548∼562bp였으며 5.8S rDNA에서는 비교종간에 서로 일치하였고 ITS I에서는 종간 뚜렷한 차이를 보였다. 2. 기주특이적 독소 생성 Alternaria spp.의 rDNA의 ITS영역의 염기서열은 비병원성인 A. alternata의 염기서열과는 서로 일치하였으나 A. brassicicola와는 3%, A. solani와는 7%의 염기서열 차를 보였다. 3. 기주특이적 독소 생성 Alternaria spp.는 비병원성 A. alternata와 진화적으로 매우 가까운 유연관계를 나타내었다. The regions of internal transcribed spacer(ITS) including 5.8S ribosomal DNA(rDXA) from six host-specific toxin(HST) producing Alternaria spp. nonpathogenic A. alternata. A. solani and A. brassicicola were sequenced to assess their relationship in taxonomy. There was no difference in the sequences of ITS including 5.8S rDNA among the HST-producing Alternaria spp. and nonpathogenic A. alternata. However, the sequences of HST-producing Alternaria spp. were different from those of A. solani and A. brassicicola by about 7% and 3%, respectively. These results suggest that HST-producing Alternaria spp. are evolutionarily very close to non pathogenic A. alternata although they have host-specific pathogenecity.
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Go, Seung Joo 한국균학회 1991 韓國菌學會誌 Vol.19 No.2
Virus-like particles were isolated from the abnormally growing culture of oyster mushroom. The virus-like particles were spherical type with 30 ㎚ from P. Florida and 23 ㎚ from P. ostreatus in diameter, respectively. The virus particles were present throughout the tissue of mycelia and spororasps. Double-stranded ribonucelic acid (ds RNA) was isolated from virus infested with P. florida as well from virus particles, but not from virus free culture. Ds RNA consisted of six segments sized 8100, 2170, 2120, 1980, 1840 and 1650 base pairs, respectively. Using the ds RNA analysis methods, the viral ds RNA segments were found from two Japanese and three European cultures, while local isolates and Korean leading cultures stored at the ASI did not contain ds RNA segment. On poplar sawdust and rice straw substrates, mycelia infected with virus grew slowly as compared to the virus free one. The yield of fruiting body infected with virus was about 30% lower than that of virus free one when they were grown on rice straw substrate. Fruiting was delayed in the mycelia infected not as infected, with compared with not free. Virus infected mycelia produced bent, thin and long stipe with the small capped fruiting body. The colour of cap infected culture was a little darker than healthy one. Branehing stipe occurred in the virus infected mycelia. The optimal growing temperature and pH of growing medium for mycelial growth of infected culture was 30℃ and around 5, respectively. It was similar to those of virus free culture. Among carbon sources used, glucose, and starch were favorable for virus free culture, while mannose was favorable for infected culture. In comparison of effects of nitrogen sources on mycelial growth virus infected and virus free cultures, arginine was the most favorable source and followed by septone and asparagine. But sulphur containing amino acids and acidic amino acids were not favorable. Cell wall composition of the virus infected mycelim of P. florida was weaker to lytic enzyme than that of virus free culture. The quantities of glucose and sucrose were higher in virus infected culture than those in virus free culture. Amino acid compositions of the infected culture were similar to those of healthy one. Viral ds RNA were transferred through mycelial anastomosis from the diseased culture to the healthy one in dikaryon. Growing characters of healthy culture after anastomosis with infected mycelia were changed like those of virus infected culture. Transmission of the dikaryon culture through mycelial anastomosis or dipping into extractions of infected culture was achieved only between intrastrains. The spores of virus infected culture contained ds RNA. The viral ds RNA segements of single spore were transferred by mating in both compatible and incompatible cultures between intrastrains.
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Transformation of Pleurotus Florida with Phleomycin Resistant Gene
Go, Seung Joo,Cha, Dong Yeul 한국균학회 1988 韓國菌學會誌 Vol.16 No.4
Transformation of P. florida has been carried out with PAN 8-1, PSFt and PAFt which contained phleomycin resistant gene. Among the protoclones, two transformed colonies from PAN 8-1 and PAFt transferred protoplasts of the anushroom have been confirmed through southern hybridization.
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Hong, Seung-Beom,Jee, Hyeong-Jin,Lee, Seung-Im,Go, Seung-Joo The Korean Society of Plant Pathology 1999 Plant Pathology Journal Vol.15 No.4
Genetic diversity of ninety-five Korean isolates of Phytophthora was investigated on the basis of PCR-RFLP of ribosomal DNA. The isolates were previously identified as following fifteen species by mycological and cultural characteristics; P. boehmeriae, P. cactorum, P. cambivora, P. capsici, P. cinnamoni, P. citricola, P. citrophthora, P. cryptogea, P. drechsleri, P. erythroseptica, P. infestans, P. megasperma, P. nicotianae, P. palmivora and P. sojae. The regions of small subunit (SSU) and internal transcribed spacer (ITS) of rDNA were amplified with primer pair, NS1 and ITS4, by polymerase chain reaction (PCR) and digested with nine restriction enzymes. P. boehmeriae, P. cactorum, P. cambivora, P. capsici, P. cinnamomi, P. citricola, P. citrphthora, P. infestans, P. nicotianae and P. palmivora showed specific band patterns for each species. However, P. sojae and P. erythroseptica presented identical band patterns and P. cryptogea, P. drechsleri and P. megasperma were divided into six groups, which were not compatible with delineation of the species. A group originated from cucurbits showed distinct band patterns from other groups, but the other five groups were closely related within 96.0% similarity, forming one complex group. Consequently, Korean isolates of Phytophthora were divided into thirteen genetic groups and each group was readily differentiated by comparing digestion patterns of AvaII, HaeIII, MboI, HhaI and MspI. Therefore, PCR-RFLP of rDNA using the five enzymes can be used to differentiate or identify the Phytophthora species reported in Korea so far.
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Hwang, Joo-Yeon,Lee, Seung Hun,Kim, Ghi-Su,Koh, Jung-Min,Go, Min-Jin,Kim, Tae-Ho,Hong, Jung-Min,Park, Eui-Kyun,Kim, Shin-Yoon,Lee, Jong-Young Korea Genome Organization 2009 Genomics & informatics Vol.7 No.1
Osteoporosis is characterized by impaired osteogenesis. BMD is a major determinant of bone strength. The role of the VDR gene in predisposition to primary osteoporosis has been recognized. However, population-based case-control studies have been reported controversial results for known candidate genes in an ethnically distinct group. To determine the genetic effects of VDR variants on osteoporosis and BMD, we directly sequenced the VDR gene in 24 unrelated Korean individuals and identified eighteen sequence variants. We investigated the potential involvement of eight SNPs in osteoporosis in postmenopausal women (n = 729). Two SNPs (LD) in intron 2, -5294G>C (rs2238135) and -4817G>A (rs17882443) showed the evidence of association with enhanced BMD of the femoral neck ($p_{additive}$=0.031 for rs2238135; $p_{additive}$=0.017 and $p_{dominant}$= 0.019 for 17882443). Moreover, VDR -4817G>A was significantly associated with protective effect on all fracture risk ($p_{recessive}$=0.035, OR=0.2, 95% CI=$0.05{\sim}0.89$), and tended to be higher BMD values at various proximal femur sites. Therefore, we suggest that the -4817G>A may be useful genetic marker for vitamin D-related metabolism and may have an important role in the increased BMD of the proximal femur in postmenopausal Korean women.
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Kim, Joo Sung,Baek, Seung Ki,Kim, Young Been,Do, Hyun Woo,Kwon, Yong Hun,Cho, Sung Woon,Yun, Young Dae,Yoon, Jae Hong,Lee, Han-Bo-Ram,Kim, Sang-Woo,Cho, Hyung Koun Elsevier 2018 Nano energy Vol.46 No.-
<P><B>Abstract</B></P> <P>Conventional <I>p-</I>CuInSe<SUB>2</SUB> absorbers for solar cells have been homogenously synthesized using multi-step process despite a narrow crystal phase region in the phase diagram and the existence of various secondary phases. In contrast, here we propose artificially-designed heterophasic blended copper indium selenide compounds for water splitting photoanodes using a simple one-step annealing synthetic process where the electrodeposited metal precursors were directly annealed with Se vapor injection and without additional intermediate steps. The resultant product is revealed to possess a novel “<I>phase-blended structure</I>” comprising two phases of <I>p-</I>type CuInSe<SUB>2</SUB> and <I>n-</I>type CuIn<SUB>3</SUB>Se<SUB>5</SUB> crystals. The CuInSe<SUB>2</SUB> nanoparticles with a higher Cu fraction are three-dimensionally (3D) embedded in the <I>n-</I>type CuIn<SUB>3</SUB>Se<SUB>5</SUB> matrix, which has been verified by various analysis methods such as X-ray diffraction, transmission electron microscopy, and capacitance-voltage curve. The average diameter of the CuInSe<SUB>2</SUB> nanoparticles is 66.8nm and the interval between the nanoparticles in the CuIn<SUB>3</SUB>Se<SUB>5</SUB> matrix is 67.6nm. Consequently, the phase-blended structure photoabsorber exhibits a remarkably enhanced anodic photocurrent of 12.7mA/cm<SUP>2</SUP> at 1.23V versus the reversible hydrogen electrode. The considerably enhanced photocurrent gain of the phase-blended structure photoanode is attributed to the excellent charge separation facilitated by the built-in potential generated from the 3D <I>p-n</I> junction.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Inorganic heterophasic blended structure was synthesized via one-step annealing process of electrodeposited Cu/In bilayers. </LI> <LI> We designed inorganic heterophasic blended structure consisting of <I>p</I>-type CuInSe<SUB>2</SUB> nanoparticles and <I>n</I>-type CuIn<SUB>3</SUB>Se<SUB>5</SUB> matrix. </LI> <LI> The nanoscale CuInSe<SUB>2</SUB> nanoparticles with a higher Cu fraction were three-dimensionally embedded in <I>n</I>-type CuIn<SUB>3</SUB>Se<SUB>5</SUB> matrix. </LI> <LI> Phase-blended structure resulted in sufficient depletion and efficiently separated photo-generated electron-hole pairs. </LI> <LI> Phase-blended structure CIS showed remarkably enhanced photocurrent and good stability with an ultra-thin Al<SUB>2</SUB>O<SUB>3</SUB> layer. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>We report an inorganic heterophasic blended structure with remarkably enhanced photocurrent performance via built-in potential between <I>p</I>-type CuInSe<SUB>2</SUB> nanoparticles and <I>n</I>-type CuIn<SUB>3</SUB>Se<SUB>5</SUB> matrix.</P> <P>[DISPLAY OMISSION]</P>
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First report of cross-species transmission of deer hepatitis E virus to a guanaco in Korea
Byung-Joo Park,Ji-Hyung Yi,Hee-Seop Ahn,Sang-Hoon Han,Yong-Hyun Kim,Hyeon-Jeong Go,Dong-Hwi Kim,Joong-Bok Lee,Seung-Yong Park,Chang-Seon Song,Sang-Won Lee,In-Soo Choi 한국예방수의학회 2017 예방수의학회지 Vol.41 No.3
The hepatitis E virus (HEV) is a leading causative agent of acute hepatitis in humans. Zoonotic HEV strains have been isolated from several animal species, including pigs. New HEV variants have been recently isolated from camels in the Middle East. In the present study, fecal samples from fallow deer, formosan deer, alpaca, and guanaco were analyzed for the detection of HEV. One HEV strain was detected from guanaco, a species of camelids. The nucleotide sequence of guanaco HEV was identical to those of deer HEV-3 strains, which implied the cross-species transmission of HEV-3 from deer to guanaco.
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