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Satish Kasturi,엄윤지,Sri Ramulu Torati,김철기 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.93 No.-
Herein, we have developed a novel high-resolution electrochemical biosensor for the detection of themicroRNA-122 (miRNA-122) using gold nanoparticles dotted reduced graphene oxide (rGO/Au)nanocomposite. The natural soapnut solution was used as a reducing agent for the synthesis of thenanocomposite. The naturally reduced rGO/Au nanocomposite was confirmed through variouscharacterization techniques. When rGO/Au nanocomposite was coated on to the gold electrode andchecked the electrochemical performance, the nanocomposite shows superior analytical performance. The probe DNA was anchored onto the binding sites of rGO/Au nanocomposite through thiol linker andrecognized the target miRNA-122. The developed rGO/Au based electrochemical biosensor demonstrateda linear response for various target miRNA-122 concentrations with a range from 10 mM to 10 pM and,with a detection limit of 1.73 pM. The developed biosensor also shows good stability and reproducibility,could be used for the detection of miRNA-122 and, also can be used for the basic research and clinicalstudies. Besides, the demonstrated rGO/Au nanocomposite-based sensing strategy could be used todetect various miRNA and protein biomarkers. Furthermore, the green synthesis approach could also beuseful for the synthesis of various nanomaterials and nanocomposites for various biomedicalapplications.
Satish Kasturi,Sri Ramulu Torati,엄윤지,김철기 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.97 No.-
Recently, miniaturized electrochemical biosensors have the advantage of real-time monitoring and labelfreedetection of biomarkers. However, controlled manipulation of reagent samples for specificimmobilization of biomarkers remains a challenge to attend high-resolution microfluidic biosensor. Here, we designed a microfluidic channel and valves integrated electrochemical biosensor for thedetection of β-amyloid (1–42) biomarker, one of the most neurotoxic peptides present in thecerebrospinalfluid, and a possible marker for the detection of Alzheimer’s disease. The formation of theantibody-antigen complexes on gold microelectrode integrated inside thefluidic chambers waspresented in the developed biosensor, and all the measurements are carefully controlled using pneumaticvalves integrated with the device. The demonstrated microfluidic electrochemical biosensor exhibits alinear response towards various β-amyloid antigen concentrations from 2.2 pM to 22 mM, with anenhanced detection limit of 1.62 pM. The developed technology offers an advantage of minimal reagentsample and suitable environment for miniaturized and sensitive detection of biomarkers, could also beused for the detection of other biomarkers with high efficiency.