Microvalve-controlled miniaturized electrochemical lab-on-a-chip based biosensor for the detection of β-amyloid biomarker

다국어 초록 (Multilingual Abstract)

Recently, miniaturized electrochemical biosensors have the advantage of real-time monitoring and labelfreedetection of biomarkers. However, controlled manipulation of reagent samples for specificimmobilization of biomarkers remains a challenge to attend high-resolution microfluidic biosensor.
Here, we designed a microfluidic channel and valves integrated electrochemical biosensor for thedetection of β-amyloid (1–42) biomarker, one of the most neurotoxic peptides present in thecerebrospinalfluid, and a possible marker for the detection of Alzheimer’s disease. The formation of theantibody-antigen complexes on gold microelectrode integrated inside thefluidic chambers waspresented in the developed biosensor, and all the measurements are carefully controlled using pneumaticvalves integrated with the device. The demonstrated microfluidic electrochemical biosensor exhibits alinear response towards various β-amyloid antigen concentrations from 2.2 pM to 22 mM, with anenhanced detection limit of 1.62 pM. The developed technology offers an advantage of minimal reagentsample and suitable environment for miniaturized and sensitive detection of biomarkers, could also beused for the detection of other biomarkers with high efficiency.

참고문헌 (Reference)

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