http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Sarang Cho,Peter Capek,Roman Bleha,Andrej Sinica,Jung Sik Lim,Jin Ree,Yong Il Park 한국당과학회 2022 한국당과학회 학술대회 Vol.2022 No.07
Many plant polysaccharide have immunomodulatory and anti-tumour activities. In the present study, water-soluble polysaccharides were isolated from an edible plant seed mainly by hot water extraction, 75% ethanol precipitation, and DEAE-cellulose column chromatography and tentatively named as PSP-F1. The PSP-F1 was shown to be a mixture of three major polysaccharides with molecular mass of approximately 1,200, 640, and 300 kDa, respectively, as estimated by size-exclusion HPLC analysis. The HPAEC-PAD analysis for neutral sugar composition and showed that PSP-F1 consists of arabinose (Ara) and xylose (Xyl), as the major neutral sugars, and other minor neutral sugars such as rhamnose (Rha), galactose (Gal), and glucose (Glc). HPLC analysis for uronic acids showed that galacturonic acid (GalA) presents as the major uronic acid and glucuronic acid (GlcA) was the minor one. Together with the neutral monosaccharides and uronic acids composition, the FT-IR and NMR analysis suggested that PSP-F1 appears to be a mixture of pectin type and heteroxylan polysaccharides. The results showed that the PSP-F1 (0-100 μg/ml) significantly induced the secretion of pro-inflammatory cytokines TNF-α and IL-6 and upregulation of MAPKs (ERK, JNK and p38) phosphorylation. The secretion of various pro-inflammatory cytokines from macrophages is mainly caused by MAPK cascade activation. These results demonstrated that the PSP-F1 isolated from a plant seed is a mixture of pectin type and heteroxylan polysaccharides, which are typical for plant cell wall polysaccharides, and that it effectively stimulates macrophages to secrete pro-inflammatory cytokines, such as TNF-α and IL-6, by upregulation of MAPKs phosphorylation. The results of this study suggests that PSP-F1 may be potential immunostimulator for health benefits and as a pharmaceutical component.
Immunostimulating activity of a water soluble polysaccharide from a defatted Korean edible nut
Sarang Cho,Juhee Shin,Seong Cheol Kim,Yong Il Park 한국당과학회 2016 한국당과학회 학술대회 Vol.2016 No.01
In this study, we extracted water soluble polysaccharides from the defatted Korean edible nut cake by boiling for 3 h in water and purified by ethanol precipitation and DEAE-cellulose column chromatography. The monosaccharide composition analysis showed that the purified polysaccharide (NP) is a galacturonoxyloarabinan type polysaccharide consisting of xylose (7.2, molar ratio), arabinose (8.8), and galacturonic acid (4.4) as major sugars and other minor sugars like glucose, galactose, rhamnose, and glucuronic acid. It's molecular mass was shown to be 1,274 kDa by size-exclusion HPLC. Then, we investigated the immunostimulating activity of NP in murine macrophage RAW 264.7 cells. The purified water soluble polysaccharide (NP) did not show any detectable level of cytotoxicity to RAW 264.7 cells in the concentration range tested (10-200 μg/ml) and rather, it promoted the cell proliferation. The NP stimulated the RAW 264.7 cells to produce the nitric oxide (NO) and increase the mRNA expression level of inducible nitric oxide synthase (iNOS). In addition, it induced the production of pro-inflammatory cytokines (TNF-α and Interleukin-6) and chemokines (RANTES and MIP-1α), which are largely known to play an important role in immune system. Taken together, these results suggest that NP can be considered a potential immunopotentiator and thus can be a potent ingredient of health beneficial functional foods or therapeutic agents.
Sarang Cho,Roman Bleha,Jeong Yeon Seo,Jisun Lee,Chang Won Lee,Andriy Synytsya,Yong Il Park 한국당과학회 2017 한국당과학회 학술대회 Vol.2017 No.01
Polysaccharides of Korean pine nut were isolated, identified and their bioactivity was evaluated. The water-soluble polysaccharides were extracted from the defatted Korean pine nut by boiling for 3 h in water and purified by ethanol precipitation and DEAE-Cellulose column chromatography. The monosaccharide composition analysis showed that purified polysaccharide(s) (PNE-P1) consisted of xylose (7.2, molar ratio), arabinose (8.8), and galacturonic acid (4.4) as major sugars and other minor sugars including glucose, galactose, rhamnose, and glucuronic acid. Their molecular weights were determined to be 1,213 kDa, 634 kDa and 331 kDa by size-exclusion HPLC. FT-IR and proton NMR analyses showed that PNE-P1 is a mixture of pectin-type polysaccharides containing arabinan, homogalacturonan (HG), rhamnogalacturonan type 1 (RG-1) and heteroxylan. The immunostimulating activity of PNE-P1 was investigated in murine macrophage RAW264.7 cells. PNE-P1 did not show any detectable level of cytotoxicity to RAW264.7 cells in the concentration range tested (10-200 μg/ml) and rather, it promoted the cell proliferation. PNE-P1 induced the production of pro-inflammatory cytokines (TNF-α, Interleukin-6 and Interleukin-10) and chemokines (RANTES and MIP-1α). These results indicate that PNE-P1 stimulated the RAW264.7 cells to increase cytokine secretions. Taken together, these results suggest that PNE-P1 can be considered as a potential immunopotentiator.
Analysis of Mobility and Safety at Intersections for Right Turn
JOKHIO, Sarang(조사랑),CHO, Yong-Bin(조용빈),KIM, Jin-tae(김진태) 대한교통학회 2018 대한교통학회 학술대회지 Vol.78 No.-
While most of the countries comply with the international conventions that prohibit traffic movement (including right turns) on the red signal, the Republic of Korea allows vehicles to turn right on red, exceptionally. The right turn traffic moving on red raises the issue of conflict between cross-directional traffic flow. This study uses VISSIM and SSAM to compare the two methods of right turn treatments: NTOR and RTOR. Further, this study is divided into two parts in terms of ‘mobility’ and ‘safety.’ Following results were obtained when the right turn method transferred from RTOR to NTOR. The delay and the travel time of the right-turn movement increased, while no changes were observed for the cross-direction through movement. The number of potential conflicts decreased in the case of the non-channelized geometric conditions, while no change is found in the case of the channelized geometric condition. In contrast to the general expectation that the conversion from RTOR to NTOR could be positive from the mobility and the safety points of view, the results delivered the opposite with the NTOR.
Chang Won Lee,Jeong Yeon Seo,Sarang Cho,Seong Cheol Kim,Yong Il Park 한국당과학회 2016 한국당과학회 학술대회 Vol.2016 No.07
Recently, researches on plant polysaccharides have been increasingly important topics for the development of therapeutics for various diseases due to their multiple biological properties including anti-inflammation, wound healing, anti-hepatitis, anti-ulcer, and anti-neoplastic effects. The Camellia oleifera has been used extensively as cooking oil and traditionally applied as a medicine for stomachache and burning injury in China. The objectives of this study was to investigate the anti-osteoporotic effect and immunostimulating activity of Camellia oleifera polysaccharides (COP). Polysaccharides were isolated from the defatted C. oleifera seeds mainly by water extraction and 75% ethanol precipitation. Monosaccharide composition analysis by HPEAC-PAD showed that these polysaccharides consist of rhamnose, arabinose, galactose, glucose, and xylose as the major neutral sugars. COP was not toxic at up to 500 μg/ml in RAW 264.7 cells, and increased immunostimulating cytokines (TNF-α and IL-6) secretion in a dose-dependent manner. In addition, COP inhibited the receptor activator of NF-кB ligand (RANKL)-induced tartrate-resistance acid phosphatase (TRAP) activity and the formation of multinucleated osteoclasts. These results suggest that the COP may have potential anti-osteoporotic activity.