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Lee, Han Saem,Kim, Ji Yun,Lee, Won Il,Kim, Sung Jin,Ko, Min Ji,Jeong, Sunjoo,Park, Keerang,Choe, Han,Lee, Heuiran Spandidos Publications] 2011 Oncology letters Vol.2 No.6
<P>Recombinant adeno-associated virus serotype 5 (rAAV5) is considered to be a promising gene transfer vehicle. However, preferential gene delivery to the tumor remains a requirement for cancer treatment. We generated rAAV5 mutants bearing tumor marker-binding peptides and analyzed their properties as viral vectors, as well as their transduction efficiencies and preferential antitumoral potencies. All of the mutants were successfully produced. Transduction analyses showed that rAAV5 mutants harboring tumor-homing peptides, including RGD and TnC, transduced human cancer cells expressing corresponding receptors on their surfaces. RGDS peptides and TnC antibodies significantly suppressed transduction by rAAV5-RGD and rAAV5-TnC. Cytotoxicity was evident upon transfer of HSV-TK to cells by re-targeted rAAV5. These results provide evidence that rAAV5 vectors, genetically armed with tumor-targeting ligands, preferentially infect human cancer cells harboring the corresponding receptors, thereby inducing antitumoral effects. Further optimization of rAAV5 mutant viruses should thus facilitate practical exploitation of these vectors for gene-based cancer treatment.</P>
Determination of hesperidin in mixed tea by HPLC
Saem Han(한샘),So-Youn Mok(목소연),Hye-Min Kim(김혜민),Jeong-Min Lee(이정민),Dong Gu Lee(이동구),Sook-Young Lee(이숙영),Jongkee Kim(김종기),Sun-Ju Kim(김선주),Sanghyun Lee(이상현) 충남대학교 농업과학연구소 2011 농업과학연구 Vol.38 No.2
The content of hesperidin in the mixed tea, which was composed of dried orange peel, laurel leaf, mulberry leaf, silver magnolia leaf, oriental melon tap, cassia seed, and licorice root, was determined by high performance liquid chromatography (HPLC). Hesperidin was quantified by a reverse phase column with gradient solvent system (watcr:acetonitrile = 80:20 to 35:65 for 30 min) and UV/VIS detection (280 ㎚). The How rate was kept constant at 1.0 ㎖/min. The content of hesperidin in the mixed tea was measured in depending on extraction time 1, 2, 3, and 4 min (29.07, 52.39, 52.45, and 88.35 ㎎/g, respectively).
Severe adenovirus pneumonia in young military man: a case report
( Saem Na Lee ),( Ji Yun Noh ),( Min Joo Choi ),( Jin Gu Yoon ),( Ji Ho Jeon ),( Eun Ju Jung ),( Seonghui Kang ),( Joon Young Song ),( Hee Jin Cheong ),( Woo Joo Kim ) 대한내과학회 2015 대한내과학회 추계학술대회 Vol.2015 No.1
Human adenoviruses (HAdVs) is a common cause of acute upper respiratory tract infection in adults, but it rarely causes respiratory failure or septic shock, severe and life-threatening infection. Although severe adenovirus pneumonia is well described in neonates, young children, and immunocompromised hosts, it is relatively unusual in healthy adults. Outbreaks of HAdV pneumonia have been described in military and long term care settings. Herein, we report an adenovirus pneumonia case in a previously healthy young military man referred from a military hospital, who needed a care in ICU. A 19-year-old healthy male patient who was in a military training session referred from military hospital due to fever with aggravated respiratory symptoms. His initial chest X ray showed air-space consolidation and centrilobular densities were on both lungs, mainly RLL. Comparing the previous chest images taken in military hospital, his pneumonia was aggravated in only a few days. Ceftriaxone and levofloxacin were given to the patient.. On hospital day 3, the result of respiratory virus PCR was reported, and all other respiratory virus were negative except adenovirus. Though antibiotics administered continuously to cover concomitant bacterial infection, there was no evidence of any bacterial infection in all respiratory specimen including broncho-alveloar lavage fluid and induced sputum. His symptom and pneumonic infiltration became with supportive cares, finally he discharged without any respiratory sequelae. In a military, among the young soldiers, outbreak of adenovirus has been reported. This case emphasizes that surveillance for respiratory illness is required in a military, because adenovirus infection can presented with severe pneumonia especially among military trainees.
Isolation and Identification of Phytochemical Constituents from Taraxacum coreanum
Lee, Sul-Lim,Han, Saem,Kim, Hye-Min,Lee, Jeong-Min,Mok, So-Youn,Lee, Sang-Hyun The Korean Society for Applied Biological Chemistr 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.1
Phytochemical constituents were isolated from the aerial parts of Taraxacum coreanum (Asteraceae) by repeated column chromatography and prep-HPLC. Their structures were identified as ${\beta}$-sitosterol (1), daucosterol (2), taraxasteryl acetate (3), chrysoeriol (4), diosmetin (5), luteolin (6), luteolin-7-O-glucoside (7), esculetin (8), and 5-hydroxypyrrolidin-2-one (9) by the interpretation of spectroscopic analyses including MS, $^1H$-, and $^{13}C$-NMR. This is the first report on the isolation of compounds 1-9 from T. coreanum. Among the compounds, 5 and 9 were isolated for the first time from Taraxacum.
Bioconverted Orostachys japonicas Extracts Suppress Angiogenic Activity of Ms-1 Endothelial Cells
Lee, Seul Gi,Kim, Jin Soo,Lee, Han-Saem,Lim, Yu-Mi,So, Jai-Hyun,Hahn, Dongyup,Ha, Yu Shin,Nam, Ju-Ock Molecular Diversity Preservation International 2017 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.18 No.12
<P>Orostachys japonicus A. Berger (), known as Wa-song in Korea, has been reported to exert various biological effects, such as anti-tumor, anti-oxidant, and anti-febrile effects. However, the anti-angiogenic effects of O. japonicus extracts remain to be investigated. In the present study, we demonstrated the anti-angiogenic effects of bioconverted O. japonicus extract (BOE) in Ms-1 mouse endothelial cells and compared them with the bioactivities of O. japonicus extract (OE). BOE, but not OE, were found to exert anti-angiogenic effects, including inhibition of cell migration, cell adhesion, tube formation of Ms-1 cells, and blood vessel formation of matrigel plug assay in vivo. Furthermore, protein levels of phosphorylated Src kinase were lower in BOE-treated cells than in OE-treated cells. Treatment with OE or BOE did not influence cell viability during the experimental period. Bioconverted extract of O. japonicus have anti-angiogenic effects in vitro and vivo, but non-bioconverted extract do not. We suggest that these observed anti-angiogenic effects are caused by the changes in the composition of bioactive compounds in the extracts as a result of biological conversion.</P>
[$Ca^{2+}-activated\;K^+$ Currents of Pancreatic Duct Cells in Guinea-pig
Lee, Han-Wook,Li, Jing Chao,Koo, Na-Youn,Piao, Zheng Gen,Hwang, Sung-Min,Han, Jae-Woong,Choi, Han-Saem,Lee, Jong-Heun,Kim, Joong-Soo,Park, Kyung-Pyo The Korean Society of Pharmacology 2004 The Korean Journal of Physiology & Pharmacology Vol.8 No.6
There are numerous studies on transepithelial transports in duct cells including $Cl^-$ and/or $HCO_3^-$. However, studies on transepithelial $K^+$ transport of normal duct cells in exocrine glands are scarce. In the present study, we examined the characteristics of $K^+$ currents in single duct cells isolated from guinea pig pancreas, using a whole-cell patch clamp technique. Both $Cl^-$ and $K^+$ conductance were found with KCI rich pipette solutions. When the bath solution was changed to low $Cl^-$, reversal potentials shifted to the negative side, $-75{\pm}4\;mV$, suggesting that this current is dominantly selective to $K^+$. We then characterized this outward rectifying $K^+$ current and examined its $Ca^{2+}$ dependency. The $K^+$ currents were activated by intracellular $Ca^{2+}$. 100 nM or 500 nM $Ca^{2+}$ in pipette significantly (P<0.05) increased outward currents (currents were normalized, $76.8{\pm}7.9\;pA$, n=4 or $107.9{\pm}35.5\;pA$, n=6) at +100 mV membrane potential, compared to those with 0 nM $Ca^{2+}$ in pipette $(27.8{\pm}3.7\;pA,\;n=6)$. We next examined whether this $K^+$ current, recorded with 100 nM $Ca^{2+}$ in pipette, was inhibited by various inhibitors, including $Ba^{2+}$, TEA and iberiotoxin. The currents were inhibited by $40.4{\pm}%$ (n=3), $87.0{\pm}%$ (n=5) and $82.5{\pm}%$ (n=9) by 1 mM $Ba^{2+}$, 5 mM TEA and 100 nM iberiotoxin, respectively. Particularly, an almost complete inhibition of the current by 100 nM iberiotoxin further confirmed that this current was activated by intracellular $Ca^{2+}$. The $K^+$ current may play a role in secretory process, slnce recycling of $K^+$ is critical for the initiation and sustaining of $CI^-$ or $HCO_3^-$ secretion in these cells.