Evaluation of Genetic Differentiation of Albizia lucida Populations from Eastern Region of the Indian Sub-continent by ISSR Markers

Subhashree Aparajita,G. R. Rout 강원대학교 산림과학연구소 2008 Journal of Forest Science Vol.24 No.1

Level and distribution of genetic diversity in seven populations of Albizia lucida Benth. in eastern region of the Indian sub-continent were estimated using ISSR markers. Relatively higher level of genetic diversity within populations was observed in seven populations of A. lucida (mean of 0.38). From the result of AMOVA, majority of genetic diversity was allocated within populations (96.2%) resulting in a moderate degree of population differentiation. The observed distribution pattern of I-SSR variant among the populations was coincided with the typical pattern of long-lived woody tree species. Genetic relationships among the populations, reconstructed by UPGMA method, revealed two genetic groups. The population of Anugul and Bargarh turned out to be the most closely related despite a distance location between them. These formations will be of great value in the development of conservation plans for species exhibiting high levels of genetic differentiation due to fragmentation, such as indication of conservation unit size, which populations should be chosen as priority in conservation plans and which samples should be introduced in areas with a low number of individuals of A. lucida.

Fresh and Hardened Properties of Fly Ash– Slag Blended Geopolymer Paste and Mortar

Subhashree Samantasinghar,Suresh Prasad Singh 한국콘크리트학회 2019 International Journal of Concrete Structures and M Vol.13 No.6

Huge amounts of by-products are generated from industrial processes which affect the environment adversely. Pro-duction process of conventional cement is energy demanding and it also emits enormous amounts of greenhouse gases. Geopolymers are the new generation green material that has a great potential of replacing the conventional cementitious materials. The fresh and hardened properties of sodium hydroxide activated binary blends of slag and fly ash based geopolymer paste and mortars are reported in this paper. Experimental outcomes on fresh and hard-ened properties such as normal consistency, flow value, setting time, drying shrinkage, soundness, and compressive strengths of the geopolymer binders are presented. Additionally, the chemical products, bonding and microstructural changes occurring during the setting and hardening course are examined. The experimental outcomes showed that the physical and mechanical properties of the binders are very much akin to that of conventional cement and the same is significantly influenced by the chemical composition of the source materials, concentration of the activa-tor and the processing environment. The consistency and setting times of geopolymers are found to be within the ranges that are prescribed for ordinary Portland cement. Highest compressive strength of around 44 MPa is obtained for slag based geopolymer mortar that is activated using 8 M sodium hydroxide solution. Fly ash and slag geopoly-mers exhibited excellent stability against expansion and shrinkage. Raw materials are optimized by design of experi-ment and the fitted model shows a good relation with the experimental data.

Assessment of Seventy-Three Rice Germplasm by Using Simple Sequence Repeats Markers

Subhashree Nayak,Jadhao Kundansingh Rajpalsingh,Debendra Nath Bastia,Kailash Chandra Samal,Gyana Ranjan Rout 한국작물학회 2014 Journal of crop science and biotechnology Vol.17 No.4

Genetic information of germplasm is the initial requirement for crop breeding programs. Rice is one of the oldest domesticatedcrop species endowed with rich genetic diversity which accounts for over 100,000 landraces and improved cultivars. The aim of thepresent study was to evaluate the genetic assessment of rice germplasm originating from India, the Philippines, China, and Malaysiathrough simple sequence repeat (SSR) markers. About 64 alleles were produced over 24 SSR primer amplifications over the wholegenome of rice. The number of alleles ranged from 1 to 4 with an average of 2.67. Out of 64 amplified bands, 58 bands were polymorphicand 6 were monomorphic bands. Most of the primers showed high polymorphic information content (PIC). The PIC valueranged from 0.53 to 0.87. The cluster analysis indicates that the 73 varieties originating from India, the Philippines, China, andMalaysia were grouped separately and made two major clusters (seven groups). Among the two major clusters, one cluster had 18genotypes which originated from the Phillipines, and China with 29% similarity with other varieties originating from India andMalaysia. Further, it was divided into two subgroups which had eight genotypes and the other had 10 genotypes with 41% similarityamong themselves. All 10 genotypes were international varieties suitable for cultivation in medium land ecosystems. The secondmajor cluster had 55 varieties including commercial rice varieties originating from India and Malaysia. The genotype ‘Swarna’ and‘Manaswini’ had 76% similarity with each other and 69% similarity with the ‘Bhanja’ & ‘Ghanteswari’ which might be the genomeassociation. The second major cluster had 55 genotypes divided into two minor groups. The first group had one genotype, i.e. ‘IR63141-B-18-B’ with 34% similarity with the other 54 genotypes. The second minor group (54 genotypes) again was divided into twogroups; one group had five genotypes with 51% similarity. Another group had 49 genotypes divided into two sub-minor groups. Based on this study, the larger range of similarity values using SSR markers provides greater confidence for the assessment of geneticrelationships among the varieties. These genotypes are suitable for cultivation in upland ecosystems. The information obtainedfrom the SSR profile helps to identify the variety diagnostic markers in 73 rice germplasm accessions. The intra- and inter-variationmight be useful for breeders to improve the rice varieties through selective breeding and cross breeding programs

Evaluation of Genetic Differentiation of Albizia lucida Populations from Eastern Region of the Indian Sub-continent by ISSR Markers

Aparajita, Subhashree,Rout, G.R. Institute of Forest Science 2008 Journal of Forest Science Vol.24 No.1

Level and distribution of genetic diversity in seven populations of Albizia lucida Benth. in eastern region of the Indian sub-continent were estimated using ISSR markers. Relatively higher level of genetic diversity within populations was observed in seven populations of A. lucida (mean of 0.38). From the result of AMOVA, majority of genetic diversity was allocated within populations (96.2%) resulting in a moderate degree of population differentiation. The observed distribution pattern of I-SSR variant among the populations was coincided with the typical pattern of long-lived woody tree species. Genetic relationships among the populations, reconstructed by UPGMA method, revealed two genetic groups. The population of Anugul and Bargarh turned out to be the most closely related despite a distance location between them. These formations will be of great value in the development of conservation plans for species exhibiting high levels of genetic differentiation due to fragmentation, such as indication of conservation unit size, which populations should be chosen as priority in conservation plans and which samples should be introduced in areas with a low number of individuals of A. lucida.

Enhanced electrochemical performance via surface sputtering on dry process-solvent-free cathode sheet for Lithium-ion batteries

박교리,( Subhashree Behera ),이경진,김현석 한국공업화학회 2023 한국공업화학회 연구논문 초록집 Vol.2023 No.0

Evaluation of Metal Biosorption Efficiency of Laboratory-grown Microcystis under Various Environmental Conditions

( PRADHAN,SUBHASHREE,SARITA SINGH,LAL CHAND RAI,DOROTHY L . PARKER ) 한국미생물생명공학회 1998 Journal of microbiology and biotechnology Vol.8 No.1

Design of Face Recognition based Embedded Home Security System

( Mrutyunjanya Sahani ),( Subhashree Subudhi ),( Mihir Narayan Mohanty ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.4

Home security has become the prime concern for everyone in present scenario. In this work an attempt has been made to develop a home security system which is accessible, affordable and yet effective.The proposed system is based on `Remote Embedded Control System` (RECS) which works both on the web and gsm platform for authentication and monitoring. This system is therefore cost effective as it relies on existing network infrastructure. As PCA is most popular and efficient algorithm for face recognition, it has been usedin this work. Next to it an interface has been developed for communication purpose in the embedded security system through the ZigBee module. Based on this embedded system, automated control of door movement has been implemented through electromagnetic door lock technology. This helps the users to monitor the real-time activities through web services/SMS. The web service consists of either web browser command or e-mail provision. The system establishes the communication between the system and authenticated user. The e-mail received by the system from the authorized person will monitor and control the real-time operation and door lock. The entire control system is reinforced using ARM1176JZF-S microcontroller and tested for actual use in the home environment. The result shows the experimental verification of the proposed system.

Genetic Relationships among 23 Ficus Accessions Using Inter- Simple Sequence Repeat Markers

Gyana Ranjan Rout,Subhashree Aparajita 한국작물학회 2009 Journal of crop science and biotechnology Vol.12 No.2

Ficus genus is widely distributed in all the climatic stages and great diversity. The exploration of genetic diversity is a pre-requisite for genome organization in the wild species and the related domesticated ones. Inter-Simple Sequence Repeat (ISSR) markers were used to assess the identification of 23 important Ficus accessions and determination of the genetic relationships among these accessions. Out of 21 ISSR primers tested, five primers produced 116 detectable fragments, of which 106 (91.3%) were polymorphic across the accessions. Each of the five primers produced a fingerprint profile unique to each of the accessions studied, and thus could be solely used for their identification. Thirteen unique bands specific to nine species were detected. These may be converted into species-specific probes for identification purposes. Genetic relationships among these accessions were evaluated by generating a similarity matrix based on the Dice coefficient and the Unweighted Pair Group Method with Arithmetic Average (UPGMA) dendogram. The results showed a clear cut separation of the 23 Ficus accessions and were in broad agreement with the morphology. Both molecular and morphological markers will be useful for preservation of the Ficus germplasm. Ficus genus is widely distributed in all the climatic stages and great diversity. The exploration of genetic diversity is a pre-requisite for genome organization in the wild species and the related domesticated ones. Inter-Simple Sequence Repeat (ISSR) markers were used to assess the identification of 23 important Ficus accessions and determination of the genetic relationships among these accessions. Out of 21 ISSR primers tested, five primers produced 116 detectable fragments, of which 106 (91.3%) were polymorphic across the accessions. Each of the five primers produced a fingerprint profile unique to each of the accessions studied, and thus could be solely used for their identification. Thirteen unique bands specific to nine species were detected. These may be converted into species-specific probes for identification purposes. Genetic relationships among these accessions were evaluated by generating a similarity matrix based on the Dice coefficient and the Unweighted Pair Group Method with Arithmetic Average (UPGMA) dendogram. The results showed a clear cut separation of the 23 Ficus accessions and were in broad agreement with the morphology. Both molecular and morphological markers will be useful for preservation of the Ficus germplasm.

Genetic Relationships among 23 Ficus Accessions Using Inter-Simple Sequence Repeat Markers

Rout, Gyana Ranjan,Aparajita, Subhashree 한국작물학회 2009 Journal of crop science and biotechnology Vol.12 No.2

Ficus genus is widely distributed in all the climatic stages and great diversity. The exploration of genetic diversity is a pre-requisite for genome organization in the wild species and the related domesticated ones. Inter-Simple Sequence Repeat (ISSR) markers were used to assess the identification of 23 important Ficus accessions and determination of the genetic relationships among these accessions. Out of 21 ISSR primers tested, five primers produced 116 detectable fragments, of which 106 (91.3%) were polymorphic across the accessions. Each of the five primers produced a fingerprint profile unique to each of the accessions studied, and thus could be solely used for their identification. Thirteen unique bands specific to nine species were detected. These may be converted into species-specific probes for identification purposes. Genetic relationships among these accessions were evaluated by generating a similarity matrix based on the Dice coefficient and the Unweighted Pair Group Method with Arithmetic Average (UPGMA) dendogram. The results showed a clear cut separation of the 23 Ficus accessions and were in broad agreement with the morphology. Both molecular and morphological markers will be useful for preservation of the Ficus germplasm.

Inhibition of Oxidative Stress and Enhancement of Cellular Activity by Mushroom Lectins in Arsenic Induced Carcinogenesis

Rana, Tanmoy,Bera, Asit Kumar,Das, Subhashree,Bhattacharya, Debasis,Pan, Diganta,Das, Subrata Kumar Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.9

Chronic arsenicosis is a major environmental health hazard throughout the world, including India. Animals and human beings are affected due to drinking of arsenic contaminated ground water, due to natural mineral deposits, arsenical pesticides or improperly disposed arsenical chemicals. Arsenic causes cancer with production of free radicals and reactive oxygen species (ROS) that are neutralized by an elaborate antioxidant defense system consisting of enzymes and numerous non-enzymatic antioxidants. Dietary antioxidant supplements are useful to counteract the carcinogenesis effects of arsenic. Oyster mushroom lectins can be regarded as ingredients of popular foods with biopharmaceutical properties. A variety of compounds have been isolated from mushrooms, which include polysaccharides and polysaccharopeptides with immune-enhancing effects. Lectins are beneficial in reducing arsenic toxicity due to anticarcinogenetic roles and may have therapeutic application in people suffering from chronic exposure to arsenic from natural sources, a global problem that is especially relevant to millions of people on the Indian subcontinent.