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      • SCIESCOPUSKCI등재

        Electrophysiological and Morphological Classification of Inhibitory Interneurons in Layer II/III of the Rat Visual Cortex

        Rhie, Duck-Joo,Kang, Ho-Young,Ryu, Gyeong-Ryul,Kim, Myung-Jun,Yoon, Shin-Hee,Hahn, Sang-June,Min, Do-Sik,Jo, Yang-Hyeok,Kim, Myung-Suk The Korean Society of Pharmacology 2003 The Korean Journal of Physiology & Pharmacology Vol.7 No.6

        Interneuron diversity is one of the key factors to hinder understanding the mechanism of cortical neural network functions even with their important roles. We characterized inhibitory interneurons in layer II/III of the rat primary visual cortex, using patch-clamp recording and confocal reconstruction, and classified inhibitory interneurons into fast spiking (FS), late spiking (LS), burst spiking (BS), and regular spiking non-pyramidal (RSNP) neurons according to their electrophysiological characteristics. Global parameters to identify inhibitory interneurons were resting membrane potential (>-70 mV) and action potential (AP) width (<0.9 msec at half amplitude). FS could be differentiated from LS, based on smaller amplitude of the AP (<∼50 mV) and shorter peak-to-trough time (P-T time) of the afterhyperpolarization (<4 msec). In addition to the shorter AP width, RSNP had the higher input resistance (>200 $M{Omega}$) and the shorter P-T time (<20 msec) than those of regular spiking pyramidal neurons. Confocal reconstruction of recorded cells revealed characteristic morphology of each subtype of inhibitory interneurons. Thus, our results provide at least four subtypes of inhibitory interneurons in layer II/III of the rat primary visual cortex and a classification scheme of inhibitory interneurons.

      • KCI등재

        Some Geometric Properties of the Weak*-integral

        RHIE GIL SEOB,PARK HI KYO 충청수학회 1990 충청수학회지 Vol.3 No.1

        We prove that if a weak*-measurable function f defined on a finite measure space into a dual Banach space is separable-like, then for every measurable set E, the weak* core of f over E is the weak* convex closed hull of the weak* essential range of f over E.

      • KCI등재

        Some Geometric Properties of the Weak<SUP>*</SUP>-integral

        Rhie, Gil-Seob,Park, Hi-Kyo 충청수학회 1990 충청수학회지 Vol.3 No.1

        We prove that if a $weak^*$-measurable function f defined on a finite measure space into a dual Banach space is separable-like, then for every measurable set E, the $weak^*$ core of f over E is the $weak^*$ convex closed hull of the $weak^*$ essential range of f over E.

      • Dietary Behavior and Food Intake of Korean Farmers in Relation to Farmers' Syndrome and Gastro-Intestinal Problem

        Rhie, Seung-Gyo,Park, Yaungja Korean Society of Community Nutrition 1999 Journal of community nutrition Vol.1 No.1

        The purpose of this study was to compare dietary behavior and food intake in relation to physical complaints, such as farmer' syndrome(FS) and gastro-intestinal(GI) problem among Korean farmers. The questionnaire was composed of 24 Cornell Medical Index (CMI), 8 farmers' syndrome, and 5 GI problem questions. Food intake data was gathered by the semi-quantitative food frequency method. The subjects(male 226, female 415) who had FS and Gi problem were 12./8% and 8.3%, respectively. The physical complaints were higher in female and the elderly group. In the FS group, lower activity was seen that in normal groups. The lower health status and fatigues were found in the physical complaint groups. Dietary behavior showed low appetite in the physical complaint group, irregular lunch in FS, and irregular breakfast in GI problem group. Changes in dietary behavior were shown in the aspects of lower amount of intake quantity(40.8%), lower consumption in fat(32.8%) and salty(38.8%) foods, and diverse food items(47.8%). Quantity of food consumed was significantly different withing groups with FS. Kinds of food consumed, intake of protein source foods, milk and calcium and total animal foods were lower in the FS group. But milk and calcium source food and all animal food intakes were higher in the GID problem group. The results suggest that dietary behavior and food intake differ within the group of physical complaints.

      • A Na<sup>+</sup>-coupled C<sub>4</sub>-dicarboxylate transporter (Asuc_0304) and aerobic growth of <i>Actinobacillus succinogenes</i> on C<sub>4</sub>-dicarboxylates

        Rhie, Mi Na,Yoon, Hyo Eun,Oh, Hye Yun,Zedler, Sandra,Unden, Gottfried,Kim, Ok Bin Society for General Microbiology 2014 Microbiology Vol.160 No.7

        <P><I>Actinobacillus succinogenes</I>, which is known to produce large amounts of succinate during fermentation of hexoses, was able to grow on C<SUB>4</SUB>-dicarboxylates such as fumarate under aerobic and anaerobic conditions. Anaerobic growth on fumarate was stimulated by glycerol and the major product was succinate, indicating the involvement of fumarate respiration similar to succinate production from glucose. The aerobic growth on C<SUB>4</SUB>-dicarboxylates and the transport proteins involved were studied. Fumarate was oxidized to acetate. The genome of <I>A. succinogenes</I> encodes six proteins with similarity to secondary C<SUB>4</SUB>-dicarboxylate transporters, including transporters of the Dcu (C<SUB>4</SUB>-dicarboxylate uptake), DcuC (C<SUB>4</SUB>-dicarboxylate uptake C), DASS (divalent anion : sodium symporter) and TDT (tellurite resistance dicarboxylate transporter) family. From the cloned genes, Asuc_0304 of the DASS family protein was able to restore aerobic growth on C<SUB>4</SUB>-dicarboxylates in a C<SUB>4</SUB>-dicarboxylate-transport-negative <I>Escherichia coli</I> strain. The strain regained succinate or fumarate uptake, which was dependent on the electrochemical proton potential and the presence of Na<SUP>+</SUP>. The transport had an optimum pH ~7, indicating transport of the dianionic C<SUB>4</SUB>-dicarboxylates. Transport competition experiments suggested substrate specificity for fumarate and succinate. The transport characteristics for C<SUB>4</SUB>-dicarboxylate uptake by cells of aerobically grown <I>A. succinogenes</I> were similar to those of Asuc_0304 expressed in <I>E. coli</I>, suggesting that Asuc_0304 has an important role in aerobic fumarate uptake in <I>A. succinogenes</I>. Asuc_0304 has sequence similarity to bacterial Na<SUP>+</SUP>-dicarboxylate cotransporters and contains the carboxylate-binding signature. Asuc_0304 was named SdcA (<U>s</U>odium-coupled C<SUB>4</SUB>-<U>d</U>i<U>c</U>arboxylate transporter from <I><U>A</U></I>. <I>succinogenes</I>).</P>

      • KCI등재

        Zu einer Typologie der Wortstellung

        Rhie, Byong Tschan 서울대학교 어학연구소 1987 語學硏究 Vol.23 No.3

        In diesim Aufsatz interessieren wir uns fu¨r den Unterschied der Wortstellung zwischen deutschen und englischen Passivsa¨tzen wie in(1). Es wird tezeigt, daβ diexem Unterschied ein allgemeines Prinzip der Wortstellung bzw. Satzbildung der beiden Sprachen ungerliegt. Dadurch wollen wir zurtypologischen Untersuchung der Wortstellung beitragen. (1) a. Marie wird von Peter geliebt. b. Mary is loved by Peter. Unter den amerikanischen Linguisten hat die Diskussion u¨ber die Typologie der Wortstellung mit greenberg (1966) begonnen (Comrie 1981 : 80). Greenberg hat verschiedene Typen der Wortstellung Klassifixiert, im Bezug auf die relative Stellung von Subjekt, Objekt und Verb, von Adjektiv und Nomen, von Haupt-und Auxiliarverb, von Standardgegenstand und Komparativen in der komparativen Konstruction und von Stamm und Affix. Die danach durchgefu¨hrten thplolgischen Untersuchungen, z. B. Lehmann(1978), bescha¨ftigen sich auch mit a¨hnlichen Problemen (Comrie 1981 : 96). Das Dertsche, dessen Grundwortstellung im Hauptsatz SVO und im Nebensatz SOV ist, wird dabei als eine typische inkonsistente Sprache angesehen (ebenda : 83; Lehmann 1978 : 409f).

      • SCISCIESCOPUS

        Substrate Water Content Influences the Flowering of Doritaenopsis Queen Beer ‘Mantefon’

        Rhie, Yong Ha,Kang, Seonghwan,Kim, Jongyun American Society for Horticultural Science 2017 HortScience Vol.52 No.12

        <P>Automated irrigation systems based on soil moisture sensor measurements can reduce water and fertilizer use while adequately meeting plant water requirements. In this study, the effects of substrate volumetric water content (θ, v/v) on the flowering of 17-month-old <I>Doritaenopsis</I> Queen Beer ‘Mantefon’ (from the time of deflasking) were examined. The plants were transplanted in plastic pots (10.5 cm width × 9.5 cm height) filled with sphagnum moss and the θ of sphagnum moss was maintained at 0.2, 0.3, 0.4, or 0.5 m<SUP>3</SUP>·m<SUP>−3</SUP> using an automated drip irrigation system. Plants grown at a θ threshold of 0.2 m<SUP>3</SUP>·m<SUP>−3</SUP> had thinner leaves and lower SPAD value than those grown at higher θ thresholds. The net CO2 uptake of the uppermost fully expanded leaf increased with increasing θ between 0.2 and 0.4 m<SUP>3</SUP>·m<SUP>−3</SUP>, but there was no significant difference in the net CO2 uptake between plants grown at 0.4 and 0.5 m<SUP>3</SUP>·m<SUP>−3</SUP> thresholds. The number of flower buds at the time of the first open flower was lower in plants grown at θ thresholds of 0.2 and 0.3 m<SUP>3</SUP>·m<SUP>−3</SUP> as compared with that in the plants grown at 0.4 and 0.5 m<SUP>3</SUP>·m<SUP>−3</SUP> thresholds. Early flower abscission, flower bud dropping, and flower senescence during the 2 weeks after flowering occurred in 55% and 30% of the plants at 0.2 and 0.3 m<SUP>3</SUP>·m<SUP>−3</SUP> thresholds, respectively, whereas plants at θ thresholds of 0.4 and 0.5 m<SUP>3</SUP>·m<SUP>−3</SUP> had negligible flower abscission. Although vegetative growth parameters were similar among θ thresholds of 0.3 m<SUP>3</SUP>·m<SUP>−3</SUP> or higher, plants grown at a θ threshold of 0.3 m<SUP>3</SUP>·m<SUP>−3</SUP> produced fewer flowers than those grown at 0.4 and 0.5 m<SUP>3</SUP>·m<SUP>−3</SUP> thresholds. During the 83-day experimental period, the system irrigated the plants ≈0.79, 1.93, 2.46, and 2.84 L/pot at θ thresholds of 0.2, 0.3, 0.4, and 0.5 m<SUP>3</SUP>·m<SUP>−3</SUP>, respectively. Overall, 0.4 m<SUP>3</SUP>·m<SUP>−3</SUP> was considered to be an optimal threshold θ level for producing high-quality <I>Doritaenopsis</I> Queen Beer ‘Mantefon’ during the flowering period with most efficient water use.</P>

      • SCOPUS

        Chondrogenic Differentiation of Human Adipose-Derived Stem Cells in PLGA (Poly(Lactic-Co-Glycolic Acid) Scaffold

        Rhie, Jong Won,Cho, Hyun Mi,Lee, Hee Young,Han, Dong Keun Trans Tech Publications, Ltd. 2007 Key Engineering Materials Vol.342-343 No.-

        <P>Human adipose tissue contain a population of pluripotent stem cells capable of differentiating along multiple mesenchymal cell lineages. The goal of this study was to examine the chondrogenic potential of adipose-derived stem (ADS) cells. ADS cells were isolated from human subcutaneous adipose tissue obtained by lipectomy and liposuction, and were expanded and grown in vitro with or without chondrogenic medium in micromass culture condition and 3D culture condition in PLGA(poly(lactic-co-glycolic acid)) scaffold. Human ADS cells of micromass culture condition and 3D culture condition in PLGA scaffolds, were differentiated with chondrogenic medium consisted of transforming growth factor-β1, insulin-transferrin-selenium, dexamethasone and ascorbate-2-phosphate. ADS cells abundantly synthesized cartilage matrix molecules including collagen type II, VI and link protein. ADS cells under high-density micromass culture condition with chondrogenic medium formed well defined nodules within 48 hours of induction. On the 3rd week after chondrogenic differentiation of ADS cells under the micromass culture condition and 3D culture condition of PLGA scaffold, mRNA of type II collagen, type VI collagen and link protein were expressed by reverse transcription polymerase chain reaction (RT-PCR). On the 3weeks, content of glycosaminoglycan in cells treated with chondrogenic medium was greater than that with non chondrogenic medium(control).On the 3rd week culture under the chondrogenic medium, hematoxylin & eosin (H & E) staining, alcian blue staining and type II collagen immunohistochemistry analysis confirmed the chondrogenic differentiation in micromass and 3D cultured specimen. These findings document the ability of ADS cells to produce characteristic cartilage matrix molecules, and provide the possibility of cartilage regeneration for cartilage substitution.</P>

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