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Cryopreservation of Citrus madurensis Zygotic Embryos by Modified Encapsulation-dehydration
Eun Gi Cho,Yue Luan Hor,Haeng Hoon Kim,Jae Gyun Gwag,V. Ramanatha Rao,Florent Engelmann 한국육종학회 2003 한국육종학회지 Vol.35 No.3
The rol e of osm otol erance and dehydr ation, in terms of both medium com posi tion and exposur e duration, on sur vi val ofem bryos of Citrus m adurensi s after cryopr eser vation usi ng the encapsul ation-dehydr ation and modified encapsul ation-dehydr
LEE, SOK-YOUNG,FAI, WAN KAH,ZAKARIA, MYRIANA,IBRAHIM, HALIJAH,OTHMAN, ROFINA YASMIN,GWAG, JAE-GYUN,RAO, V. RAMANATHA,PARK, YONG-JIN Blackwell Publishing Ltd 2007 Molecular ecology notes Vol.7 No.6
<P>Abstract</P><P>The present study reports isolation and characterization of eight polymorphic microsatellite markers for <I>Zingiber officinale</I> Rosc. (Ginger). A total of 34 alleles were detected across the 20 accessions, with an average of 4.3 alleles per locus. Values for observed and expected heterozygosities ranged from 0 to 1.0 and from 0.23 to 0.67, respectively. The heterozygote deficits were observed at three loci. At the significance threshold (<I>P</I> < 0.05) of the eight loci, seven were found to have deviated from Hardy–Weinberg equilibrium, whereas significant linkage disequilibria were observed between 10 pairs of loci. Our data indicate the existence of moderate level of genetic diversity among the ginger accessions genotyped with eight markers.</P>
Analysis of Genetic Diversity of Korean Wheat Cultivars Using Microsatellite DNA Polymorphisms
Park,Yong-Jin,Cho,Gyu-Taek,Ma,Kyung-Ho,Lee,Sok-Young,Lee,Jung-Ro,Kim,Young-Chang,Cho,Eun-Gi,Kim Chang-Yung,Nam,Jung-Hyun,Rao,V,Ramanatha,Kang,Hee-Kyoung 한국자원식물학회 2004 Plant Resources Vol.7 No.2
Genetic background and phylogenetic relationships among 20 Korean wheat cultivars were assessed using microsatellites after amplifying with 13 SSR primer pairs. Average allele number per primer pair was 3.36. Genetic similarities for every pair of cultivars ranged from 0.42 to 0.97, with 0.69 of overall average. Korean cultivars were divided into two major groups based on microsatellite DNA polymorphisms. Group I consisted of relatively old cultivars developed until 1970s, and group II contained the recent cultivars developed during 1980s and 1990s. Amongst old elite cultivars/lines, ‘Yukseung 3’, ‘Norin 12’ and ‘Norin 72’ contributed most to the genetic background of cultivars belonging to group I, and ‘Norin 4’, ‘Norin 12’, ‘Norin 43’ and ‘Norin 72’ to group II, respectively. The phylogenetic relationship of Korean wheat cultivars was in accordance with the genealogical data of each cultivar. The genetic background of each cultivar was assessed from the point of breeding and germplasm management such as variety identification and duplicated accessions for assisting in developing a system for the registration of new variety based on the molecular characterization in future.