A Simple and Robustness Algorithm for ECG R- peak Detection

Rahman, Md Saifur,Choi, Chulhyung,Kim, Young-pil,Kim, Sikyung The Korean Institute of Electrical Engineers 2018 Journal of Electrical Engineering & Technology Vol.13 No.5

There have been numerous studies that extract the R-peak from electrocardiogram (ECG) signals. All of these studies can extract R-peak from ECG. However, these methods are complicated and difficult to implement in a real-time portable ECG device. After filtration choosing a threshold value for R-peak detection is a big challenge. Fixed threshold scheme is sometimes unable to detect low R-peak value and adaptive threshold sometime detect wrong R-peak for more adaptation. In this paper, a simple and robustness algorithm is proposed to detect R-peak with less complexity. This method also solves the problem of threshold value selection. Using the adaptive filter, the baseline drift can be removed from ECG signal. After filtration, an appropriate threshold value is automatically chosen by using the minimum and maximum value of an ECG signals. Then the neighborhood searching scheme is applied under threshold value to detect R-peak from ECG signals. Proposed method improves the detection and accuracy rate of R-peak detection. After R-peak detection, we calculate heart rate to know the heart condition.

Simplified the Screening and In Vitro Appraisal of Antioxidant, Cytotoxic, Thrombolytic, Antimicrobial and Membrane Stabilizing Activities of Lablab Purpures at a Time

Rahman, M. Saifur,Uddin, M. Gias,Alam, M. Badrul,Yoo, Jin Cheol The Basic Science Institute Chosun University 2014 조선자연과학논문집 Vol.7 No.3

To simplify the different biological investigation of the methanolic extract and solvent-solvent partitioning of Lablab purpures (L. purpures) bark. In-vitro anti-oxidant study was determined using total DPPH radical scavenging assay. In vitro antimicrobial study was measured by observing zone of inhibition. The cytotoxic activity was studied using brine shrimp lethality bioassay and thrombolytic activity by clot disruption method. The antioxidant potential was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and Folin-Ciocalteau reagents using butylated hydroxytolune (BHT) and ascorbic acid as standards. The Aqueous soluble fraction revealed the highest free radical scavenging activity ($IC_{50}=48.76{\mu}g/mL$). The antimicrobial screening of the bark of L. purpures exhibited mild to moderate activity in test microorganisms. The CSF showed the maximum relative percentage inhibition against Salmonella parathyphi (34.2%) for bacteria and C. albicans (28.8%) for fungi whereas, lowest relative percentage inhibition against Sarcina lutea (22.0%) for bacteria and Aspergillus niger (24.4%) for fungi. In the brine shrimp lethality bioassay, The $LC_{50}$ values of Carbon tetrachloride and N-Hexane soluble fraction were found $92.18{\mu}g/mL$, and $68.95{\mu}g/mL$ respectively while the $LC_{50}$ values of standard Vincristine sulphate was $1.37{\mu}g/mL$. The methanolic extract and its organic soluble fractions of Lablab purpureus at concentration 2.0 mg/mL, significantly protected the lysis of erythrocyte membrane induced by hypotonic solution and heat as compared to the standard, acetyl salicylic acid (0.10 mg/mL). The MSF and AQSF produced 61.48 % and 53.75% inhibition of hemolysis of RBC caused by hypotonic solution respectively, whereas acetyl salicylic acid (0.10 mg/mL) showed 76.42%. Ethanol extract of L. purpures and all of its different partitions exhibited moderate thrombolytic activity of 37.25%-2.40%. Very good preliminary screening and simplified experiments were able to show the different biological activity of methanolic extract and its soluble fractions of L. purpures at a time.

On the Definition of Intuitionistic Fuzzy h-ideals of Hemirings

Rahman, Saifur,Saikia, Helen Kumari Department of Mathematics 2013 Kyungpook mathematical journal Vol.53 No.3

Using the Lukasiewicz 3-valued implication operator, the notion of an (${\alpha},{\beta}$)-intuitionistic fuzzy left (right) $h$-ideal of a hemiring is introduced, where ${\alpha},{\beta}{\in}\{{\in},q,{\in}{\wedge}q,{\in}{\vee}q\}$. We define intuitionistic fuzzy left (right) $h$-ideal with thresholds ($s,t$) of a hemiring R and investigate their various properties. We characterize intuitionistic fuzzy left (right) $h$-ideal with thresholds ($s,t$) and (${\alpha},{\beta}$)-intuitionistic fuzzy left (right) $h$-ideal of a hemiring R by its level sets. We establish that an intuitionistic fuzzy set A of a hemiring R is a (${\in},{\in}$) (or (${\in},{\in}{\vee}q$) or (${\in}{\wedge}q,{\in}$)-intuitionistic fuzzy left (right) $h$-ideal of R if and only if A is an intuitionistic fuzzy left (right) $h$-ideal with thresholds (0, 1) (or (0, 0.5) or (0.5, 1)) of R respectively. It is also shown that A is a (${\in},{\in}$) (or (${\in},{\in}{\vee}q$) or (${\in}{\wedge}q,{\in}$))-intuitionistic fuzzy left (right) $h$-ideal if and only if for any $p{\in}$ (0, 1] (or $p{\in}$ (0, 0.5] or $p{\in}$ (0.5, 1] ), $A_p$ is a fuzzy left (right) $h$-ideal. Finally, we prove that an intuitionistic fuzzy set A of a hemiring R is an intuitionistic fuzzy left (right) $h$-ideal with thresholds ($s,t$) of R if and only if for any $p{\in}(s,t]$, the cut set $A_p$ is a fuzzy left (right) $h$-ideal of R.

Evaluation of Abelmoschus Esculentus Seed for Antioxidant and Phytochemical Analysis Using In vitro Assays

Rahman, Md. Saifur,Yoo, Jin Cheol The Basic Science Institute Chosun University 2014 조선자연과학논문집 Vol.7 No.4

The aim of this study was to assess the in vitro potential of methanolic seed extract of Abelmoschus esculentus as a natural antioxidant. The DPPH activity of the Ethyl acetate soluble fraction (10, 20, 40, 80, and $160{\mu}g/mL$) was increased in a dose dependent manner, which was found in the range of 18.97-90.47% as compared to ascorbic acid 26.44-93.71%. The $IC_{50}$ values of Ethyl acetate soluble fraction (EAES) and ascorbicacid in DPPH radical scavenging assay were obtained to be 28.12 and $18.43{\mu}g/mL$, respectively. Measurement of polyphenol content of the EAES of A. esculentus seed was achieved using Folin-Ciocalteau reagent containing 53.80 mg/g of total phenolic content, which was found signicantly higher when compared to reference standard gallic acid. Similarly total flavonoids and proabthocyanidis of EAES and chloroform soluble fraction (CAES) were found significantly 147 mg/g and 14.24 mg/g respectively when both compared to reference standard quercetin. EAES exhibited high significant lipid peroxidation inhibition effects in a dose-dependent manner, with $IC_{50}$ values of $38.08{\mu}g/mL$, whereas, standard quercetin, with $IC_{50}$ value of $36.67{\mu}g/mL$. All extract/fractions showed dose dependent reducing power ability and these differences were statistically significant (p<0.001). The results obtained in this study clearly indicate that A. esculentus seed has a signicant potential to use as a natural antioxidant agent.

A novel antioxidant peptide, purified from <i>Bacillus amyloliquefaciens</i>, showed strong antioxidant potential via Nrf-2 mediated heme oxygenase-1 expression

Rahman, Md. Saifur,Hee Choi, Yun,Seok Choi, Yoon,Alam, Md. Badrul,Han Lee, Sang,Cheol Yoo, Jin Elsevier 2018 Food chemistry Vol.239 No.-

<P><B>Abstract</B></P> <P>A novel antioxidant peptide YD1 (∼1.0kDa), purified from the strain <I>Bacillus amyloliquefaciens CBSYD1</I>, displayed activity in several <I>in vitro</I> assays and was also efficient against Gram-positive, Gram-negative as well as multidrug-resistant (MDR) bacteria. Strain growth was adapted to bile-salt conditions where a clear halos-zone was observed in a bile-salt plate assay and was viable in different digestive track conditions. YD1 treatment on RAW 264.7 cells increased the transcriptional and translational activities of NF-E2-related factor-2 (Nrf-2) through the enhanced levels of heme oxygenase-1 (HO-1). Furthermore, the YD1-treated group showed higher levels of antioxidant enzymes compared to the oxidative stress group. YD1 demonstrated a strong antioxidant activity by decreasing nitric oxide (NO) and reactive oxygen species (ROS) in RAW 264.7 cells. This study suggests that YD1 and the strain could be a natural antioxidant and a probiotic candidate respectively.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A novel glycine-rich antioxidant peptide purified from <I>Bacillus amyloliquefaciens.</I> </LI> <LI> Showed antioxidant potential via Nrf-2 mediated heme oxygenase-1 expression. </LI> <LI> YD1 displayed active against gram-positive, negative and resistant bacteria. </LI> </UL> </P>

Simplified the Screening and In Vitro Appraisal of Antioxidant, Cytotoxic, Thrombolytic, Antimicrobial and Membrane Stabilizing Activities of Lablab Purpures at a Time

M. Saifur Rahman,M. Gias Uddin,M. Badrul Alam,Jin Cheol Yoo 조선대학교 기초과학연구원 2014 조선자연과학논문집 Vol.7 No.3

Simplified the Screening and In Vitro Appraisal of Antioxidant, Cytotoxic, Thrombolytic, Antimicrobial and Membrane Stabilizing Activities of Lablab Purpures at a Time

M. Saifur Rahman, M. Gias Uddin, M. Badrul Alam, Jin Cheol Yoo 조선대학교 기초과학연구원 2014 조선자연과학논문집 Vol.7 No.3

To simplify the different biological investigation of the methanolic extract and solvent-solvent partitioning of Lablab purpures (L. purpures) bark. In-vitro anti-oxidant study was determined using total DPPH radical scavenging assay. In vitro antimicrobial study was measured by observing zone of inhibition. The cytotoxic activity was studied using brine shrimp lethality bioassay and thrombolytic activity by clot disruption method. The antioxidant potential was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and Folin-Ciocalteau reagents using butylated hydroxytolune (BHT) and ascorbic acid as standards. The Aqueous soluble fraction revealed the highest free radical scavenging activity (IC50 = 48.76 μg/mL). The antimicrobial screening of the bark of L. purpures exhibited mild to moderate activity in test microorganisms. The CSF showed the maximum relative percentage inhibition against Salmonella parathyphi (34.2%) for bacteria and C. albicans (28.8%) for fungi whereas, lowest relative percentage inhibition against Sarcina lutea (22.0%) for bacteria and Aspergillus niger (24.4%) for fungi. In the brine shrimp lethality bioassay, The LC50 values of Carbon tetrachloride and N-Hexane soluble fraction were found 92.18 μg/mL, and 68.95 μg/mL respectively while the LC50 values of standard Vincristine sulphate was 1.37 μg/mL. The methanolic extract and its organic soluble fractions of Lablab purpureus at concentration 2.0 mg/mL, significantly protected the lysis of erythrocyte membrane induced by hypotonic solution and heat as compared to the standard, acetyl salicylic acid (0.10 mg/mL). The MSF and AQSF produced 61.48 % and 53.75% inhibition of hemolysis of RBC caused by hypotonic solution respectively, whereas acetyl salicylic acid (0.10 mg/mL) showed 76.42%. Ethanol extract of L. purpures and all of its different partitions exhibited moderate thrombolytic activity of 37.25%-2.40%. Very good preliminary screening and simplified experiments were able to show the different biological activity of methanolic extract and its soluble fractions of L. purpures at a time.

A Simple and Robustness Algorithm for ECG R- peak Detection

Md Saifur Rahman,ChulhyungChoi,Young-pil Kim,Sikyung Kim 대한전기학회 2018 Journal of Electrical Engineering & Technology Vol.13 No.5

There have been numerous studies that extract the R-peak from electrocardiogram (ECG) signals. All of these studies can extract R-peak from ECG. However, these methods are complicated and difficult to implement in a real-time portable ECG device. After filtration choosing a threshold value for R-peak detection is a big challenge. Fixed threshold scheme is sometimes unable to detect low R-peak value and adaptive threshold sometime detect wrong R-peak for more adaptation. In this paper, a simple and robustness algorithm is proposed to detect R-peak with less complexity. This method also solves the problem of threshold value selection. Using the adaptive filter, the baseline drift can be removed from ECG signal. After filtration, an appropriate threshold value is automatically chosen by using the minimum and maximum value of an ECG signals. Then the neighborhood searching scheme is applied under threshold value to detect R-peak from ECG signals. Proposed method improves the detection and accuracy rate of R-peak detection. After R-peak detection, we calculate heart rate to know the heart condition.

Evaluation of Abelmoschus Esculentus Seed for Antioxidant and Phytochemical Analysis Using In vitro Assays

Md. Saifur Rahman,Jin Cheol Yoo 조선대학교 기초과학연구원 2014 조선자연과학논문집 Vol.7 No.4

Identification and Characterization of a Novel Pectinolytic Enzyme CBS 31 Produced by Bacillus subtilis Isolated from Fermented Vegetable

Md. Saifur RAHMAN,Jin Cheol YOO 한국생물공학회 2016 한국생물공학회 학술대회 Vol.2016 No.10