Trachyspermum ammi (L.) fruit essential oil influencing on membrane permeability and surface characteristics in inhibiting food-borne pathogens

Paul, S.,Dubey, R.C.,Maheswari, D.K.,Kang, S.C. Butterworths ; Taylor Francis ; Elsevier Science 2011 Food control Vol.22 No.5

This study was aimed to evaluate the anti-bacterial potential of the essential oil and extracts of Trachyspermum ammi fruits against food borne and spoilage bacteria. The chemical compositions of the oil were analyzed by GC-MS. Thirty compounds representing 91.39% of the total oil were identified. The extracellular ATP concentration, release of cell constituents and potassium ions from the bacterial cell were measured after treatment with essential oil of T. ammi at MIC concentration. The oil, and extracts of hexane, chloroform, ethyl acetate and methanol of T. ammi fruits displayed remarkable anti-bacterial effects against Bacillus subtilis ATCC 6633, Pseudomonas aeruginosa KCTC 2004, Salmonella typhimurium KCTC 2515, Enterobactor aerogens KCTC 2190 and Staphylococcus aureus ATCC 6538. The scanning electron microscopic studies also demonstrated inhibitory effect of the oil on the morphology of B. subtilis ATCC 6633 at the MIC concentration, along with the potential effect on cell viabilities of the tested bacteria.

SAMPLING BASED UNCERTAINTY ANALYSIS OF 10 % HOTLEG BREAK LOCA IN LARGE SCALE TEST FACILITY

SAMIRAN SENGUPTA,S. K. DUBEY,R. S. RAO,S. K. GUPTA,V. K RAINA 한국원자력학회 2010 Nuclear Engineering and Technology Vol.42 No.6

Sampling based uncertainty analysis was carried out to quantify uncertainty in predictions of best estimate codeRELAP5/MOD3.2 for a thermal hydraulic test (10% hot leg break LOCA) performed in the Large Scale Test Facility (LSTF)as a part of an IAEA coordinated research project. The nodalisation of the test facility was qualified for both steady state andtransient level by systematically applying the procedures led by uncertainty methodology based on accuracy extrapolation(UMAE); uncertainty analysis was carried out using the Latin hypercube sampling (LHS) method to evaluate uncertainty forten input parameters. Sixteen output parameters were selected for uncertainty evaluation and uncertainty band between 5thand 95thpercentile of the output parameters were evaluated. It was observed that the uncertainty band for the primary pressure duringtwo phase blowdown is larger than that of the remaining period. Similarly, a larger uncertainty band is observed relating toaccumulator injection flow during reflood phase. Importance analysis was also carried out and standard rank regression coefficientswere computed to quantify the effect of each individual input parameter on output parameters. It was observed that the breakdischarge coefficient is the most important uncertain parameter relating to the prediction of all the primary side parametersand that the steam generator (SG) relief pressure setting is the most important parameter in predicting the SG secondary pressure.

Subfertility in Males: An Important Cause of Bull Disposal in Bovines

Mukhopadhyay, C.S.,Gupta, A.K.,Yadav, B.R.,Khate, K.,Raina, V.S.,Mohanty, T.K.,Dubey, P.P. Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.4

The study had two objectives, namely, to estimate the andrological disorders leading to disposal of Karan Fries (KF), Sahiwal cattle and Murrah buffalo bulls and to study the effect of various factors (species/breeds, season of birth and period of birth) on male reproductive parameters. Records on occurrence of subfertility problems and disposal pattern of bulls maintained at the National Dairy Research Institute herd were collected for 15 years (1991 to 2005). Percentage of bulls producing freezable semen was less in the crossbred cattle (58.46%) as compared to Sahiwal (81.69%) and Murrah bulls (81.05%). Various subfertility traits like poor libido and unacceptable seminal profile were found to be the significant reasons (p<0.01) for culling of the breeding bulls. Inadequate sex drive was the main contributing factor for bull disposal in Sahiwal (22.55%) and Murrah bulls (15.12%) whereas poor semen quality and freezability were most frequently observed in KF bulls (24.29 and 7.29 percent, respectively). Least squares analyses of different male reproductive parameters showed that species/breeds had significant effect (p<0.05) on all traits except for frozen semen production periods (FSPP). Periods of birth were significantly different (p<0.05) for all traits except for semen volume. Age at first semen collection (AFSC), age at first semen freezing (AFSF) and age at disposal (AD) were highest in Murrah, while frozen semen production period (FSPP) and semen production period (SPP) were highest in KF and lowest in Sahiwal. The age at first semen donation and breeding period could be reduced by introducing the bulls to training at an early age. These results revealed a declining trend in AFSC, AFSF, FSPP, SPP and AD, thereby indicating an improvement in reproductive performance over the years. The age at first semen donation in bovines can be reduced by introducing the young male calves to training at an early age, which could increase the dosage of semen obtained from each male.

SAMPLING BASED UNCERTAINTY ANALYSIS OF 10 % HOT LEG BREAK LOCA IN LARGE SCALE TEST FACILITY

Sengupta, Samiran,Dubey, S.K.,Rao, R.S.,Gupta, S.K.,Raina, V.K Korean Nuclear Society 2010 Nuclear Engineering and Technology Vol.42 No.6

Sampling based uncertainty analysis was carried out to quantify uncertainty in predictions of best estimate code RELAP5/MOD3.2 for a thermal hydraulic test (10% hot leg break LOCA) performed in the Large Scale Test Facility (LSTF) as a part of an IAEA coordinated research project. The nodalisation of the test facility was qualified for both steady state and transient level by systematically applying the procedures led by uncertainty methodology based on accuracy extrapolation (UMAE); uncertainty analysis was carried out using the Latin hypercube sampling (LHS) method to evaluate uncertainty for ten input parameters. Sixteen output parameters were selected for uncertainty evaluation and uncertainty band between $5^{th}$ and $95^{th}$ percentile of the output parameters were evaluated. It was observed that the uncertainty band for the primary pressure during two phase blowdown is larger than that of the remaining period. Similarly, a larger uncertainty band is observed relating to accumulator injection flow during reflood phase. Importance analysis was also carried out and standard rank regression coefficients were computed to quantify the effect of each individual input parameter on output parameters. It was observed that the break discharge coefficient is the most important uncertain parameter relating to the prediction of all the primary side parameters and that the steam generator (SG) relief pressure setting is the most important parameter in predicting the SG secondary pressure.

Protective Role of Mentha arvensis Aqueous Extract against Manganese Induced Toxicity by Reducing Mn Translocation and Promoting Antioxidative Defense in growing Indica Rice Seedlings

Ritika Rajpoot,Anjana Rani,Rajneesh Kumar Srivastava,Poonam Pandey,R. S. Dubey 한국작물학회 2018 Journal of crop science and biotechnology Vol.21 No.4

Mentha arvensis L. (Ma) leaf extract, a wide source of natural antioxidants is widely used to protect animal cells against oxidative stress. In the current study, we have studied the ameliorative effects of Ma leaf extract on Mn induced oxidative stress in rice (Oryza sativa L.) seedlings. Hydroponically grown rice seedlings treated with 1.5 mM MnCl2 showed a decline in vigour and contents of photosynthetic pigments, increased production of reactive oxygen species (O2˙‾, H2O2, and .OH), lipid peroxidation, protein thiol and carbonyl contents along with increased activity of antioxidative enzymes- superoxide dismutase (SOD), guaiacol peroxidase (GPX) and ascorbate peroxidase (APX). The activity of catalase (CAT), however, declined in the seedlings after prolonged Mn treatment of 8 days. Interestingly exogenous application of Ma leaf extract (638.4 mg ml-1) effectively restored the Mn-induced decline in seedling vigour, photosynthetic pigments, increased levels of H2O2, lipid peroxides and altered activities of antioxidative enzymes. The results indicate that exogenous application of Ma leaf extract significantly lowers the toxic effects of Mn in rice seedlings by modulating Mn translocation and reducing oxidative stress.