EXISTENCE OF SOLUTIONS FOR BOUNDARY BLOW-UP QUASILINEAR ELLIPTIC SYSTEMS

Miao, Qing,Yang, Zuodong The Korean Society for Computational and Applied M 2010 Journal of applied mathematics & informatics Vol.28 No.3

In this paper, we are concerned with the quasilinear elliptic systems with boundary blow-up conditions in a smooth bounded domain. Using the method of lower and upper solutions, we prove the sufficient conditions for the existence of the positive solution. Our main results are new and extend the results in [Mingxin Wang, Lei Wei, Existence and boundary blow-up rates of solutions for boundary blow-up elliptic systems, Nonlinear Analysis(In Press)].

Link Prediction Algorithm for Signed Social Networks Based on Local and Global Tightness

( Miao-miao Liu ),( Qing-cui Hu ),( Jing-feng Guo ),( Jing Chen ) 한국정보처리학회 2021 Journal of information processing systems Vol.17 No.2

Given that most of the link prediction algorithms for signed social networks can only complete sign prediction, a novel algorithm is proposed aiming to achieve both link prediction and sign prediction in signed networks. Based on the structural balance theory, the local link tightness and global link tightness are defined respectively by using the structural information of paths with the step size of 2 and 3 between the two nodes. Then the total similarity of the node pair can be obtained by combining them. Its absolute value measures the possibility of the two nodes to establish a link, and its sign is the sign prediction result of the predicted link. The effectiveness and correctness of the proposed algorithm are verified on six typical datasets. Comparison and analysis are also carried out with the classical prediction algorithms in signed networks such as CN-Predict, ICN-Predict, and PSNBS (prediction in signed networks based on balance and similarity) using the evaluation indexes like area under the curve (AUC), Precision, improved AUC′, improved Accuracy′, and so on. Results show that the proposed algorithm achieves good performance in both link prediction and sign prediction, and its accuracy is higher than other algorithms. Moreover, it can achieve a good balance between prediction accuracy and computational complexity.

Characterization and Antioxidant Activity of Released Exopolysaccharide from Potential Probiotic Leuconostoc mesenteroides LM187

( Qing Zhang ),( Jie Wang ),( Qing Sun ),( Shu-ming Zhang ),( Xiang-yang Sun ),( Chan-yuan Li ),( Miao-xin Zheng ),( Wen-liang Xiang ),( Jie Tang ) 한국미생물 · 생명공학회 2021 Journal of microbiology and biotechnology Vol.31 No.8

A released exopolysaccharide (rEPS)-producing strain (LM187) with good acid resistance, bile salt resistance, and cholesterol-lowering properties was isolated from Sichuan paocai and identified as Leuconostoc mesenteroides subsp. mesenteroides. The purified rEPS, designated as rEPS414, had a uniform molecular weight of 7.757 × 10⁵ Da. Analysis of the monosaccharide composition revealed that the molecule was mainly composed of glucose. The Fourier transform-infrared spectrum showed that rEPS414 contained both α-type and β-type glycosidic bonds. ¹H and ¹³C nuclear magnetic resonance spectra analysis showed that the purified rEPS contained arabinose, galactose, and rhamnose, but less uronic acid. Scanning electron microscopy demonstrated that the exopolysaccharide displayed a large number of scattered, fluffy, porous cellular network flake structures. In addition, rEPS414 exhibited strong in vitro antioxidant activity. These results showed that strain LM187 and its rEPS are promising probiotics with broad prospects in industry.

EXISTENCE OF SOLUTIONS FOR BOUNDARY BLOW-UP QUASILINEAR ELLIPTIC SYSTEMS

Qing Miao,Zuodong Yang 한국전산응용수학회 2010 Journal of applied mathematics & informatics Vol.28 No.3

In this paper, we are concerned with the quasilinear elliptic systems with boundary blow-up conditions in a smooth bounded domain. Using the method of lower and upper solutions,we prove the sufficient conditions for the existence of the positive solution. Our main results are new and extend the results in [Mingxin Wang, Lei Wei, Existence and boundary blow-up rates of solutions for boundary blow-up elliptic systems, Nonlinear Analysis(In Press)].

Coupling of Lever Arm Swing and Biased Brownian Motion in Actomyosin

Nie, Qing-Miao,Togashi, Akio,Sasaki, Takeshi N.,Takano, Mitsunori,Sasai, Masaki,Terada, Tomoki P. Public Library of Science 2014 PLoS computational biology Vol.10 No.4

<▼1><P>An important unresolved problem associated with actomyosin motors is the role of Brownian motion in the process of force generation. On the basis of structural observations of myosins and actins, the widely held lever-arm hypothesis has been proposed, in which proteins are assumed to show sequential structural changes among observed and hypothesized structures to exert mechanical force. An alternative hypothesis, the Brownian motion hypothesis, has been supported by single-molecule experiments and emphasizes more on the roles of fluctuating protein movement. In this study, we address the long-standing controversy between the lever-arm hypothesis and the Brownian motion hypothesis through <I>in silico</I> observations of an actomyosin system. We study a system composed of myosin II and actin filament by calculating free-energy landscapes of actin-myosin interactions using the molecular dynamics method and by simulating transitions among dynamically changing free-energy landscapes using the Monte Carlo method. The results obtained by this combined multi-scale calculation show that myosin with inorganic phosphate (P<SUB>i</SUB>) and ADP weakly binds to actin and that after releasing P<SUB>i</SUB> and ADP, myosin moves along the actin filament toward the strong-binding site by exhibiting the biased Brownian motion, a behavior consistent with the observed single-molecular behavior of myosin. Conformational flexibility of loops at the actin-interface of myosin and the N-terminus of actin subunit is necessary for the distinct bias in the Brownian motion. Both the 5.5–11 nm displacement due to the biased Brownian motion and the 3–5 nm displacement due to lever-arm swing contribute to the net displacement of myosin. The calculated results further suggest that the recovery stroke of the lever arm plays an important role in enhancing the displacement of myosin through multiple cycles of ATP hydrolysis, suggesting a unified movement mechanism for various members of the myosin family.</P></▼1><▼2><P><B>Author Summary</B></P><P>Myosin II is a molecular motor that is fueled by ATP hydrolysis and generates mechanical force by interacting with actin filament. Comparison among various myosin structures obtained by X-ray and electron microscope analyses has led to the hypothesis that structural change of myosin in ATP hydrolysis cycle is the driving mechanism of force generation. However, single-molecule experiments have suggested an alternative mechanism in which myosin moves stochastically in a biased direction along actin filament. Computer simulation serves as a platform for assessing these hypotheses by revealing the prominent features of the dynamically changing landscape of actin-myosin interaction. The calculated results show that myosin binds to actin at different locations of actin filament in the weak- and strong-binding states and that the free energy has a global gradient from the weak-binding site to the strong-binding site. Myosin relaxing into the strong-binding state therefore necessarily shows the biased Brownian motion toward the strong-binding site. Lever-arm swing is induced during this relaxation process; therefore, lever-arm swing and the biased Brownian motion are coupled to contribute to the net displacement of myosin. This coupling should affect the dynamical behaviors of muscle and cardiac systems.</P></▼2>

Radiosensitivity Enhancement by Arsenic Trioxide in Conjunction with Hyperthermia in the EC-1 Esophageal Carcinoma Cell Line

Cui, Yan-Hui,Liang, Hai-Jun,Zhang, Qing-Qin,Li, Si-Qing,Li, Xiao-Rui,Huo, Xiao-Qing,Yang, Qing-Hui,Li, Wei-Wei,Gu, Jian-Fa,Hua, Qin-Liang,Lu, Ping,Miao, Zhan-Hui Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4

Objective: To explore the effect on radiosensitivity of arsenic trioxide ($As_20_3$) in conjunction with hyperthermia on the esophageal carcinoma EC-1 cell line. Method: Inhibition of EC-1 cell proliferation at different concentrations of $As_20_3$ was assessed using the methyl thiazolyl blue colorimetric method (MTT method), with calculation of $IC_{50}$ value and choice of 20% of the $IC_{50}$ as the experimental drug concentration. Blank control, $As_20_3$, hyperthermia, radiotherapy group, $As_20_3$ + hyperthermia, $As_20_3$ + radiotherapy, hyperthermia + radiotherapy and $As_20_3$ + hyperthermia + radiotherapy groups were established, and the cell survival fraction (SF) was calculated from flat panel colony forming analysis, and fitted by the 'multitarget click mathematical model'. Flow cytometry (FCM) was used to detect changes in cell apoptosis and the cell cycle. Results: $As_20_3$ exerted inhibitory effects on proliferation of esophageal carcinoma EC-1 cells, with an $IC_{50}$ of 18.7 ${\mu}mol/L$. After joint therapy of $As_20_3$ + hyperthermia + radiotherapy, the results of FCM showed that cells could be arrested in the $G_2$/M phase, and as the ratio of cells in $G_0/G_1$ and S phases decreased, cell death became more pronounced. Conclusion: $As_20_3$ and hyperthermia exert radiosensitivity effects on esophageal carcinoma EC-1 cells, with synergy in combination. Mechanistically, $As_20_3$ and hyperthermia mainly influence the cell cycle distribution of EC-1 esophageal carcinoma cells, decreasing the repair of sublethal damage and inducing apoptosis, thereby enhancing the killing effects of radioactive rays.

Single Nucleotide Polymorphisms of the GnRHR Gene Associated with Reproductive Traits of Japanese Flounder (Paralichthys olivaceus)

He, Feng,Wen, Hai-Shen,Li, Ji-Fang,Yu, Da-Hui,Ma, Rui-Qin,Shi, Dan,Mu, Wei-Jie,Zhang, Yuan-Qing,Hu, Jian,Liu, Miao,Han, Wei-Guo,Zhang, Jia-Nan,Wang, Qing-Qing,Yuan, Yu-Ren,Liu, Qun Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.4

Gonadotropin-releasing hormone receptor (GnRHR) gene is expressed at the anterior pituitary gland and plays a key role in gonad development. This study aimed to investigate molecular genetic characteristics of the GnRHR gene and elucidate the effects of single nucleotide polymorphisms (SNPs) of GnRHR gene on sex steroid level in Japanese flounder (Paralichthys olivaceus). We used polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) and sequencing of the GnRHR gene in 75 individuals. We identified three SNPs in the GnRHR gene: P1 locus (C759A and C830T) in the coding region of exon2 which were both linked together and P2 locus (G984T) in the coding region of exon3, which added a new transcript factor (ADR1) and a new methylation site (CG). Only C830T of P1 leads to amino acid changes Thr266Ile. Statistical analysis showed that P1 was significantly associated with $17{\beta}$-estradiol ($E_2$) level (p<0.01) and gonadosomatic index (GSI) (p<0.05). Individuals with genotype BB of P1 had significantly higher serum $E_2$ levels (p<0.01) and GSI (p<0.05) than those of genotype AA or AB. Another SNP, P2, synonymous mutation, was significantly associated with GSI (p<0.05). Individuals with genotype AB of P2 had significantly higher GSI (p<0.05) than that of genotype AA. In addition, there was a significant association between one diplotype based on three SNPs and reproductive traits. The genetic effects for both serum $E_2$ level and GSI of diplotype D4 were super diplotypes (p<0.05). These results suggest that the SNPs in Japanese Flounder GnRHR are associated with $E_2$ level and GSI.

Expression and Regulation of Transcription Factor FoxA2 in Chronic Rhinosinusitis With and Without Nasal Polyps

Qing Luo,Jia Zhang,Hongtian Wang,Fenghong Chen,Xi Luo,Beiping Miao,Xingmei Wu,Renqiang Ma,Xiangqian Luo,Geng Xu,Jianbo Shi,Huabin Li 대한천식알레르기학회 2015 Allergy, Asthma & Immunology Research Vol.7 No.5

Purpose: Chronic rhinosinusitis (CRS) is characterized by the excessive production of mucus. However, the molecular mechanism underlying mucin overproduction in CRS with or without nasal polyps (CRSwNP and CRSsNP, respectively) is poorly understood. This study was conducted to assess the importance of the transcription factor FoxA2 in mucin production and to investigate the targeting of FoxA2 as a potential therapeutic strategy for mucus hypersecretion in CRS patients. Methods: We enrolled 15 CRSwNP patients, 15 CRSsNP patients, and 10 normal controls in this study. The expression levels of FoxA2, MUC5AC, and MUC5B in inflamed and healthy nasal tissues were examined via immunohistochemistry and quantitative reverse transcription-polymerase chain reaction, and the levels of several proinflammatory cytokines in nasal secretions were measured via FlowCytomix analysis. In addition, the expression of MUC5AC and FoxA2 was determined in polyp-derived epithelial cells and NCI-H292 cells after in vitro stimulation. Results: FoxA2 was significantly down-regulated, and MUC5AC and MUC5B were significantly up-regulated in both the CRSwNP and CRSsNP patients compared to the controls (P<0.05), and the protein level of FoxA2 was negatively associated with the IL-6 level in the CRS patients (P<0.05). IL-6 significantly increased MUC5AC expression but inhibited FoxA2 expression in vitro (P<0.05). Transfection with a FoxA2 expression plasmid significantly decreased MUC5AC promoter activity (P<0.05) and inhibited IL-6-induced MUC5AC production (P<0.05). In addition, clarithromycin significantly alleviated IL-6-induced FoxA2 suppression and decreased MUC5AC expression in vitro (P<0.05). Conclusions: Our findings suggest that FoxA2 may be considered a therapeutic target for the modulation of mucus hypersecretion in CRS patients.

Semantic Behavior of Indexed Co-inductive Data Type in Fibrational Setting

Miao De-cheng,Xi Jian-qing,Jiang Chang-jin,Liang Yong-lin 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.6

Traditional methods such as category theory and coalgebra have some drawbacks to analyze semantic behavior of indexed co-inductive data type. Aiming at the problem above, this paper explored indexed co-inductive data type in programming by Fibrations theory. Our main work was that we firstly made some basic logical structures of indexed co-inductive data type over a fibration such as truth and quotient functor; using endo-functor in base categories and their equation-preserving lifting in total categories, then we analyzed semantic behavior of indexed co-inductive data type; at last we briefly introduced applications of Fibrations theory on indexed co-inductive data type by example. Compared with traditional methods, brief descriptions and flexible expansibility of Fibrations theory can analyze semantic behavior of indexed co-inductive data type accurately, and superior abstractness of Fibrations theory doesn’t rely on particular computing environments to compute semantics.

Purification and Characterization of a Laccase from the Edible Wild Mushroom Tricholoma mongolicum

( Miao Li ),( Guo Qing Zhang ),( He Xiang Wang ),( Tzibun Ng ) 한국미생물 · 생명공학회 2010 Journal of microbiology and biotechnology Vol.20 No.7