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Kim, Pyong-Kih,Kim, Jae-Won,Park, Jeonghwan The Korean Society of Fisheries and Aquatic Scienc 2018 Fisheries and Aquatic Sciences Vol.21 No.1
This study evaluated hemato-histological changes of black porgy in recirculating aquaculture systems (RAS) with three different ozone doses (no ozone, 20 g, and $40g\;ozone/kg\;feed\;day^{-1}$). During the 44-day study, black porgy did not show significant behavior changes or mortalities in both the ozonated systems displaying average total residual oxidants concentrations of 0.12 and 0.25 mg/L. There were no differences in growth and blood parameters among the systems. However, histological alterations on gills and livers were observed in both the treatment systems. In the higher ozone dose, signs of cellular damage were more apparent. Although the ozone doses did not manifest a serious adverse effect on growth and hematological observations in this short-term study, an ozone dose should not exceed $20g\;ozone/kg\;feed\;day^{-1}$ for black porgy based on the histological result. In order to use ozone in a seawater RAS, further studies will be needed to evaluate long-term effects of total residual oxidants.
( Pyong-kih Kim ),( Jae-won Kim ),( Jeonghwan Park ) 한국수산과학회(구 한국수산학회) 2018 Fisheries and Aquatic Sciences Vol.21 No.1
This study evaluated hemato-histological changes of black porgy in recirculating aquaculture systems (RAS) with three different ozone doses (no ozone, 20 g, and 40 g ozone/kg feed day<sup>-1</sup>). During the 44-day study, black porgy did not show significant behavior changes or mortalities in both the ozonated systems displaying average total residual oxidants concentrations of 0.12 and 0.25 mg/L. There were no differences in growth and blood parameters among the systems. However, histological alterations on gills and livers were observed in both the treatment systems. In the higher ozone dose, signs of cellular damage were more apparent. Although the ozone doses did not manifest a serious adverse effect on growth and hematological observations in this short-term study, an ozone dose should not exceed 20 g ozone/kg feed day-1 for black porgy based on the histological result. In order to use ozone in a seawater RAS, further studies will be needed to evaluate long-term effects of total residual oxidants.
순치 기간에 따른 육봉형 산천어(Oncorhynchus masou masou )의 해수 적응능력
김병기 ( Pyong Kih Kim ),김재원 ( Jae Won Kim ),박정환 ( Jeong Hwan Park ),성기백 ( Ki Baik Seong ),김현주 ( Hyeon Ju Kim ) 한국수산과학회(구 한국수산학회) 2011 한국수산과학회지 Vol.44 No.6
The seawater adaptability of land-locked masu salmon Oncorhynchus masou masou via acclimation was examined for aquaculture purposes. The survival, blood chemistry, and histological changes of masu salmon (150 g) were measured after 7-, 15-, and 30-day acclimation periods. After a total of 60 days in seawater cultures that incorporated the various acclimation periods, survival was 83.5, 87.2, and 91.0% for the 7-, 15-, and 30-day periods, respectively; thus, survival increased with longer periods of acclimation. Feeding efficiencies were 32.1, 52.0, and 40.6% for the 7-, 15-, and 30-day periods, and specific growth rates were 0.14, 0.26, and 0.23%, respectively. Generally, masu salmon appeared to exhibit better growth performance after an acclimation period of 15 days. Cortisol concentrations [mean ± SD] for 7, 15, and 30 days of acclimation were 21.0 ± 6.5, 17.8 ± 4.8, and 21.2 ± 5.4 μg/dl, with the lowest values occurring with 15 days of acclimation. Osmolarities were 359.2 ± 26.1, 350.4 ± 29.2, and 354.6 ± 29.3 mOsm/kg, and glucose concentrations were 60.7 ± 20.7, 72.9 ± 17.3, and 76.6 ± 14.1 mg/dl for the 7-, 15-, and 30-day acclimation periods, respectively (P < 0.05). The histological study revealed that both gills and both kidneys of the masu salmon exhibited middle- to end-stage and middle-stage lesions in the 7- and 15-day groups, respectively, whereas these organs only had early-stage lesions in the 30-day group in the final experiment. Therefore, the seawater acclimation of masu salmon should involve more than 30 days in seawater.
대두박 사료에 대한 무지개송어( Oncorhynchus mykiss )의 소화 특성
김병기 ( Kim Pyong Kih ),전중균 ( Joong Kyun Jeon ) 한국수산과학회 2014 한국수산과학회지 Vol.47 No.6
To evaluate the digestive characteristics and bioavailability of dietary soybean meal (SBM), the post-prandial changes in the gastric contents of rainbow trout Oncorhynchus mykiss were determined for 24 h after feeding of SBM diets. A curve estimation of regression diagnostics using a comparison of the adjusted r2 and probability was performed to test the tendency of the post-prandial changes and gave a quadratic polynomial (exponential) regression for all experimental groups. The gastric evacuations rates (GER) for higher-SBM groups were slower than those for lowerSBM groups. The estimated GER (digestion time) for 75% gastric content for fish fed a 70% SBM diet was 1.63 times longer than that for fish fed the control diet. Despite the fact that the pH values in the gastric contents rose from 4.05 at 0 h to 5.12-5.38 at 1 h after feeding, then dropped to 4.57-4.83 at 9 h, with no significant differences among experimental groups, the gastric moisture contents increased significantly in the higher-SBM groups. This is most likely due to an increase in digestive juices in the higher-SBM groups, rather than water introduced externally. The percentage of soluble nitrogen in the gastric contents of fish fed the higher-SBM diets was higher than that in the fish fed the control diet, and the SBRs (stomach weight/ body weight×100) in the higher-SBM groups (diets 4, 5, 6 and 7) were also significantly higher than the SBR of the control group ( P <0.05). This may indicate that the protein in SBM can be digested slowly due to physiological digestive adaptation in rainbow trout.
순환여과시스템에서 사육밀도와 용존산소 농도가 돌돔(Oplegnathus fasciatus )의 성장과 혈액성상에 미치는 영향
김병기 ( Pyong Kih Kim ) 한국수산과학회(구 한국수산학회) 2011 한국수산과학회지 Vol.44 No.6
The parrotfish Oplegnathus fasciatus is a sub-tropical species that is difficult to culture during the winter in South Korea. As a result, a recirculating aquaculture system (RAS) was developed to rear parrotfish. This study investigated the effects of stocking density and dissolved oxygen (DO) concentration on the growth and hematology of the parrotfish in the RAS. The experimental stocking densities were 5 (SD05), 10 (SD10), 15 (SD15), and 20 kg/m3 (SD20) total body weight to tank water volume. As the stocking density increased, the mean weight gain, feed efficiency, and specific growth rate tended to decrease. However, SD10 and SD15 did not differ statistically from SD05 in feed efficiency (P>0.05). Although better growth was achieved in SD05, SD10 and SD15 appeared to be acceptable, practically and economically, in terms of feed efficiency. The experimental DO concentration ranges were 3-4 (DO3), 5-6 (DO5), and 7-8 mg/L (DO7). The mean weight gain and survival tended to increase with the DO concentration, but there were no differences among treatments (P>0.05). Although the feed efficiency did not differ among the treatments, the specific growth rates and daily feed intake increased with the DO concentration and were the highest in DO7 (P<0.05). The cortisol concentration was the highest in DO3 (P<0.05), while there was no difference between DO5 and DO7 (P>0.05).