Optimization of Medium for the Production of a Novel Aquaculture Probiotic, Micrococcus MCCB 104 Using Central Composite Design

Preetha, R.,Jayaprakash, N.S.,Philip, Rosamma,Singh, I.S. Bright Korean Society for Biotechnology and Bioengineerin 2007 Biotechnology and Bioprocess Engineering Vol.12 No.5

A marine isolate of Micrococcus MCCB 104 has been identified as an aquaculture probiotic antagonistic to Vibrio. In the present study different carbon and nitrogen sources and growth factors in a mineral base medium were optimized for enhanced biomass production and antagonistic activity against the target pathogen, Vibrio harveyi, following response surface methodology (RSM). Accordingly the minimum and maximum limits of the selected variables were determined and a set of fifty experiments programmed employing central composite design (CCD) of RSM for the final optimization. The response surface plots of biomass showed similar pattern with that of antagonistic activity, which indicated a strong correlation between the biomass and antagonism. The optimum concentration of the carbon sources, nitrogen sources, and growth factors for both biomass and antagonistic activity were glucose (17.4 g/L), lactose (17 g/L), sodium chloride (16.9 g/L), ammonium chloride (3.3 g/L), and mineral salts solution (18.3 mL/L).

Price Forecasting on a Large Scale Data Set using Time Series and Neural Network Models

Preetha K G,K R Remesh Babu,Sangeetha U,Rinta Susan Thomas,Saigopika,Shalon Walter,Swapna Thomas 한국인터넷정보학회 2022 KSII Transactions on Internet and Information Syst Vol.16 No.12

Environment, price, regulation, and other factors influence the price of agricultural products, which is a social signal of product supply and demand. The price of many agricultural products fluctuates greatly due to the asymmetry between production and marketing details. Horticultural goods are particularly price sensitive because they cannot be stored for long periods of time. It is very important and helpful to forecast the price of horticultural products which is crucial in designing a cropping plan. The proposed method guides the farmers in agricultural product production and harvesting plans. Farmers can benefit from long-term forecasting since it helps them plan their planting and harvesting schedules. Customers can also profit from daily average price estimates for the short term. This paper study the time series models such as ARIMA, SARIMA, and neural network models such as BPN, LSTM and are used for wheat cost prediction in India. A large scale available data set is collected and tested. The results shows that since ARIMA and SARIMA models are well suited for small-scale, continuous, and periodic data, the BPN and LSTM provide more accurate and faster results for predicting well weekly and monthly trends of price fluctuation.

Induction of in vitro ketosis condition and suppression using methylsulfonylmethane by altering ANGPTL3 expression through STAT5b signaling mechanism

Sreekantan Preetha, Nipin,Kang, Dong Young,Darvin, Pramod,Kim, Don Nam,Joung, Youn Hee,Kim, Sang Yoon,Cho, Kwang Hyun,Do, Chang Hee,Park, Kyung Do,Lee, Joon-Ho,Lee, Hyun Young,Park, Jong Hwan,Yang, Yo Taylor Francis 2015 Animal cells and systems Vol.19 No.1

Effect of cationic precursor pH on optical and transport properties of SILAR deposited nano crystalline PbS thin films

K.C. Preetha,K.V. Murali,A.J. Ragina,K. Deepa,T.L. Remadevi 한국물리학회 2012 Current Applied Physics Vol.12 No.1

The lead sulfide (PbS) thin films were deposited on glass substrate using successive ionic layer adsorption and reaction (SILAR) method at different pH of the cationic precursor, keeping the pH of the anionic precursor invariant. In this work, we establish that the pH of the cationic precursor and in turn the size of the crystallites affects the optical and electrical properties of PbS thin films. The characterization of the film was carried out using X-ray diffraction, scanning electron microscopy, optical and electrical measurement techniques. The presence of nanocrystallites was revealed by optical absorption and structural measurements. The PbS thin films obtained under optimal deposition conditions were found to be polycrystalline with face centered cubic structure. The lattice parameter, grain size, micro strain,average internal stress and dislocation density in the film were calculated and correlated with pH of the solution. The values of average crystallite size were found to be in the range 16e23 nm. Optical studies revealed the existence of direct and indirect band gap values in the range 0.99e1.84 eV and 0.60e0.92 eV, respectively. The room temperature resistivity of the synthesized PbS films was in the range of 1.2 ×107 to 3.5×107Ω cm.

An Energy Efficient Intelligent Method for Sensor Node Selection to Improve the Data Reliability in Internet of Things Networks

( Remesh Babu K R ),( Preetha K G ),( Saritha S ),( Rinil K R ) 한국인터넷정보학회 2021 KSII Transactions on Internet and Information Syst Vol.15 No.9

Internet of Things (IoT) connects several objects with embedded sensors and they are capable of exchanging information between devices to create a smart environment. IoT smart devices have limited resources, such as batteries, computing power, and bandwidth, but comprehensive sensing causes severe energy restrictions, lowering data quality. The main objective of the proposal is to build a hybrid protocol which provides high data quality and reduced energy consumption in IoT sensor network. The hybrid protocol gives a flexible and complete solution for sensor selection problem. It selects a subset of active sensor nodes in the network which will increase the data quality and optimize the energy consumption. Since the unused sensor nodes switch off during the sensing phase, the energy consumption is greatly reduced. The hybrid protocol uses Dijkstra’s algorithm for determining the shortest path for sensing data and Ant colony inspired variable path selection algorithm for selecting active nodes in the network. The missing data due to inactive sensor nodes is reconstructed using enhanced belief propagation algorithm. The proposed hybrid method is evaluated using real sensor data and the demonstrated results show significant improvement in energy consumption, data utility and data reconstruction rate compared to other existing methods.

Carcinoma Thyroid-Have Turned Around the Corner?

N. Dorairajan,M.D.<SUP>1<.SUP>,Preetha Muthayya,M.D. and Srinivasan,M.D. 대한갑상선-내분비외과학회 2002 The Koreran journal of Endocrine Surgery Vol.2 No.1

Thyroid surgery was revolutionized by Theodre Kocher and his work earned him the Nobel Prize in 1909. The techniques laid down by him hold good even today, even in thyroid cancer surgery. William Halstead, Charles Mayo, George Crile, Frank Lahey and Thomas Dunhill also made significant contributions in vastly improving the outcome of surgery of Thyroid disease. FNAC, a technique first performed by Greig and Gray in 1904 to aspirate trypanosomes from the lymph nodes of patients with sleeping sickness, is the best modality for work up of thyroid nodules and has eclipsed Radio Active Iodine Uptake study. After the widespread use of FNAC in thyroid disease since 1970s a pre operative tissue diagnosis was possible in most thyroid nodules. The role of FNAC is further enhanced by combining Immuno Histo chemistry and nuclear DNA analysis (Petra Werga et al, Karolinska hospital, Sweden, 2000). Yield is highest when done by persons well versed with this technique. The only distinct failure of FNAC is its inability to distinguish between follicular adenoma and carcinoma. But even this has been overcome with the advent of techniques like the following. Two dimensional proton Magnetic Resonance Spectroscopic analysis has now enabled one to effectively identify malignancy as well as to differentiate between Follicular adenoma and carcinoma and is based increased cell surface fucosylation (Wanda. B Mackinnon et al, NSW, Australia). Molecular genetics in relation to thyroid disease especially in carcinoma thyroid had exploded many myths and realities. The relationship between RET-PTC oncogene and MTC is established beyond doubt and signaled the induction of "prophylactic thyroidectomy" in the realm of surgeons. Prophylactic thyroidectomy is ideally done in RET-PTC positive individuals at six years of age. Central compartment lymph node dissection is added if there is a raised serum calcium or if the patient is older than 10 years. Bilateral lymph node dissection is done in patents older than 15 with elevated serum calcitonin (Henning Dralle et al, Germany 1998). An Association between TSHR and Gs mutation with toxic adenosis has also been proven. P 53 mutation plays a dominant role in the development of anaplastic carcinoma (Diana Learoyd et al, NSW, Australia, 2000). Familial Papillary Thyroid Carcinoma [FPTC] and MNG has also been recognized as a distinct entity apart from other familial syndromes like Gardener's syndrome and Cowden's disease. Primary criteria for susceptibility to FPTC include PTC in two first degree relatives, MNG in three first or second degree relatives , patients younger than 33 years with multi focal, bilateral or metastatic PTC (Thomas.Musholt et al, Hannover, Germany 2000). Surgical techniques are opted based on various scoring systems like AMES, MACIS, AGES. These systems help prognosticate thyroid cancer based on age, size of tumor, extra thyroidal spread, metastasis and completeness of thyroidectomy. Sentinel lymph node mapping was first with isosulfan blue dye is safe and sentinel nodes even if present in the lateral cervical compartments and mediastinum are detected (Elijah Dixon et al, Alberta, Canada 2000). Microdissection with central compartment lymph nodal clearance has been shown to positively influence survival and decrease local recurrence in patients with PTC (Lars-Erik Tissel et al, Goteberg, Sweden 1996). Complications of surgery like permanent Recurrent laryngeal nerve palsy [1%] and hypo parathyroidism [2%] have been reduced and recurrence scaled down significantly because of well established endocrine centers and training of young surgeons (Tom Reeve, NSW Australia, 2000). Complications are higher in re operative thyroid surgeries with RLN palsy as high as 2% and hypoparathyroidism seen in almost 5% of the cases (Puzzolo et al, Italy 1997). (Korean J Endocrine Surg 2002;2:5-9)

Optimization of Medium for theProduction of a Novel AquacultureProbiotic, Micrococcus MCCB 104 UsingCentral Composite Design

I. S. Bright Singh,R. Preetha,N. S. Jayaprakash,Rosamma Philip 한국생물공학회 2007 Biotechnology and Bioprocess Engineering Vol.12 No.5

A marine isolate of Micrococcus MCCB 104 has been identified as an aquaculture probiotic antagonistic to Vibrio. In the present study different carbon and nitrogen sources and growth factors in a mineral base medium were optimized for enhanced biomass production and antagonistic activity against the target pathogen, Vibrio harveyi, following response surface methodology (RSM). Accordingly the minimum and maximum limits of the selected variables were determined and a set of fifty experiments programmed employing central composite design (CCD) of RSM for the final optimization. The response surface plots of biomass showed similar pattern with that of antagonistic activity, which indicated a strong correlation between the biomass and antagonism. The optimum concentration of the carbon sources, nitrogen sources, and growth factors for both biomass and antagonistic activity were glucose (17.4 g/L), lactose (17 g/L), sodium chloride (16.9 g/L), ammonium chloride (3.3 g/L), and mineral salts solution (18.3 mL/L).

Induction of in vitro root tubers in Holostemma annulare (Roxb.) K. Schum. for the production of bioactive metabolites

Padmavathi Amma Somasekharan Nair Smitha Devi,Achuthan Sudarsanan Hemanthakumar,Thankappan Suvarna Preetha 한국식물생명공학회 2022 JOURNAL OF PLANT BIOTECHNOLOGY Vol.49 No.3

Holostemma annulare (Family Asclepiadaceae) is an invaluable vulnerable medicinal plant; the root tubers are used in Ayurveda medicine and by folk healers to treat various ailments. In this study, Schenk and Hildebrandt medium fortified with the cytokinins 6-benzyl adenine, kinetin, and auxins, including indole 3-butyric acid, indole 3-acetic acid, α-naphthaleneacetic acid, and 2,4-dichlorophenoxyacetic acid, were checked for their efficiency on root tuber induction from different explants. Adventitious root tubers were more successfully induced from in vitro leaf segments and shoots when cultured in Schenk and Hildebrandt medium supplemented with 0.5 mg/l of α -naphthaleneacetic acid. In addition, preliminary phytochemical analysis of in vitro root tubers and identification of different secondary metabolites were conducted. Thin layer chromatography and high performance thin layer chromatography analysis of the crude methanolic extracts of the in vitro root tuber identified the presence of lupeol, a bioactive triterpene. Adventitious root tuber induction offers a novel method for the in vitro production of bioactive metabolites that can be scaled up by bioreactors, thus ensuring the conservation and sustainable utilization of H. annulare. The study warrants further scale-up production and pharmacological investigation that can be extended for pharmaceutical needs.

An insight into in vitro strategies for bioproduction of isoflavones

Vidya Nandakumar,Saravanan Krishnagowdu,Halka Jayachandran,Kowsalya Kumaresan,Preetha Jaganathan Sakthi Yazhini,Gurusaravanan Packiaraj,Radhakrishnan Ramalingam,Nanthini A. Usha Raja,Arun Muthukrishna 한국식물생명공학회 2021 Plant biotechnology reports Vol.15 No.6

Isoflavones (ISF) are potent secondary metabolites, widely distributed among the members of the Fabaceae family. They have a multitude of biological functions in plants, animals, and humans. Most of the functions exhibited by isoflavones are highly beneficial to human beings. Benefits mainly include lowering cardiovascular diseases, reducing menopausal symptoms, and decreasing the risk of hormone-related cancers. Therefore, this potent compound has emerged as a promising possibility for making medicines and has grabbed the pharmaceutical industry's attention. Nowadays, many isoflavone-based products are manufactured commercially as food supplements, and their demand is drastically increasing globally. Numerous in vitro stud- ies have successfully synthesized value-added metabolites that possess a vast number of industrial applications. Production of isoflavones within the plants depends on several biological and environmental conditions. Hence, in vitro cultures pro- vide an alternate source to recover these compounds independent of environmental factors and limited bio-resources. Plant organ cultures could be highly recommended as a reliable platform for synthesizing plant-based pharmaceutically active isoflavones. Moreover, biotechnological approaches, such as elicitation and precursor feeding, can eventually promote the yield and increase these metabolites' production. This review initially outlines information regarding the source, structure, synthesis, and diverse roles of isoflavones. Later, this paper describes different plant organ cultures for the in vitro production of isoflavones and highlights elicitation strategies as well as metabolic engineering work to enhance production.

A study on transport and plugging of sodium aerosol in leak paths of concrete blocks

Narayanam Sujatha Pavan,Sen Soubhadra,Kumari Kalpana,Kumar Amit,Pujala Usha,Subramanian V.,Chandrasekharan S.,Preetha R.,Venkatraman B. 한국원자력학회 2024 Nuclear Engineering and Technology Vol.56 No.1

In the event of a severe accident in Sodium Cooled Fast Reactors (SFR), the sodium combustion aerosols along with fission product aerosols would migrate to the environment through leak paths of the Reactor Containment Building (RCB) concrete wall under positive pressure. Understanding the characteristics of sodium aerosol transport through concrete leak paths is important as it governs the environmental source term. In this context, experiments are conducted to study the influence of various parameters like pressure, initial mass concentration, leak path diameter, humidity etc., on the transport and deposition of sodium aerosols in straight leak paths of concrete. The leak paths in concrete specimens are prepared by casting and the diameter of the leak path is measured using thermography technique. Aerosol transport experiments are conducted to measure the transported and plugged aerosol mass in the leak paths and corresponding plugging times. The values of differential pressure, aerosol concentration and relative humidity taken for the study are in the ranges 10–15 kPa, 0.65–3.04 g/m3 and 30–90% respectively. These observations are numerically simulated using 1-Dimensional transport equation. The simulated values are compared with the experimental results and reasonable agreement among them is observed. From the safety assessment view of reactor, the approach presented here is conservative as it is with straight leak paths.