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- 저자 : N. S. Jayaprakash (검색결과 4 건)
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I. S. Bright Singh,R. Preetha,N. S. Jayaprakash,Rosamma Philip 한국생물공학회 2007 Biotechnology and Bioprocess Engineering Vol.12 No.5
A marine isolate of Micrococcus MCCB 104 has been identified as an aquaculture probiotic antagonistic to Vibrio. In the present study different carbon and nitrogen sources and growth factors in a mineral base medium were optimized for enhanced biomass production and antagonistic activity against the target pathogen, Vibrio harveyi, following response surface methodology (RSM). Accordingly the minimum and maximum limits of the selected variables were determined and a set of fifty experiments programmed employing central composite design (CCD) of RSM for the final optimization. The response surface plots of biomass showed similar pattern with that of antagonistic activity, which indicated a strong correlation between the biomass and antagonism. The optimum concentration of the carbon sources, nitrogen sources, and growth factors for both biomass and antagonistic activity were glucose (17.4 g/L), lactose (17 g/L), sodium chloride (16.9 g/L), ammonium chloride (3.3 g/L), and mineral salts solution (18.3 mL/L).
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Preetha, R.,Jayaprakash, N.S.,Philip, Rosamma,Singh, I.S. Bright Korean Society for Biotechnology and Bioengineerin 2007 Biotechnology and Bioprocess Engineering Vol.12 No.5
A marine isolate of Micrococcus MCCB 104 has been identified as an aquaculture probiotic antagonistic to Vibrio. In the present study different carbon and nitrogen sources and growth factors in a mineral base medium were optimized for enhanced biomass production and antagonistic activity against the target pathogen, Vibrio harveyi, following response surface methodology (RSM). Accordingly the minimum and maximum limits of the selected variables were determined and a set of fifty experiments programmed employing central composite design (CCD) of RSM for the final optimization. The response surface plots of biomass showed similar pattern with that of antagonistic activity, which indicated a strong correlation between the biomass and antagonism. The optimum concentration of the carbon sources, nitrogen sources, and growth factors for both biomass and antagonistic activity were glucose (17.4 g/L), lactose (17 g/L), sodium chloride (16.9 g/L), ammonium chloride (3.3 g/L), and mineral salts solution (18.3 mL/L).
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Trends in Monoclonal Antibody Production Using Various Bioreactor Systems
( I. Jyothilekshmi ),( N. S. Jayaprakash ) 한국미생물생명공학회(구 한국산업미생물학회) 2021 Journal of microbiology and biotechnology Vol.31 No.3
Monoclonal antibodies are widely used as diagnostic reagents and for therapeutic purposes, and their demand is increasing extensively. To produce these proteins in sufficient quantities for commercial use, it is necessary to raise the output by scaling up the production processes. This review describes recent trends in high-density cell culture systems established for monoclonal antibody production that are excellent methods to scale up from the lab-scale cell culture. Among the reactors, hollow fiber bioreactors contribute to a major part of high-density cell culture as they can provide a tremendous amount of surface area in a small volume for cell growth. As an alternative to hollow fiber reactors, a novel disposable bioreactor has been developed, which consists of a polymer-based supermacroporous material, cryogel, as a matrix for cell growth. Packed bed systems and disposable wave bioreactors have also been introduced for high cell density culture. These developments in high-density cell culture systems have led to the monoclonal antibody production in an economically favourable manner and made monoclonal antibodies one of the dominant therapeutic and diagnostic proteins in biopharmaceutical industry.
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R. Ravichandran,Venkatesh Padmanabhan,M. A. Vijayalakhsmi,N. S. Jayaprakash 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.1
Lactoferrin is one of the important nutraceuticalwith several physiological and biological functions. Bovinecolostrum contains higher concentrations of lactoferrinthan in the milk. The present work describes the use of twomixed mode sorbents, mercapto ethyl pyridine (MEPHyperCel™) and phenyl propyl amine (PPA HyperCel™),for the recovery of lactoferrin from bovine colostrumwhey. The dynamic binding capacity study showed that, inMEP the mode of interaction of lactoferrin is similar toIgG and had a more or less same binding capacity of~20 mg/mL of sorbent. In PPA, the interaction of lactoferrinwas hydrophobic and was influenced by the addition ofsalt. The binding capacity of lactoferrin with PPA in absenceand presence of salt was found to be ~17 and ~18 mg/mLsorbent respectively. The binding buffers used for thechromatographic experiments were 0.05 M sodium phosphatebuffer, pH 7.4, with or without sodium chloride (0.15 M). A decreasing step pH gradient of pH 6.0, 5.5 and 4.0 wasperformed for elution. Both lactoferrin and immunoglobulinwere obtained with high homogeneity. The recoveredlactoferrin was confirmed by immunoblot analysis. The chromatographicelution fractions obtained from MEP Hyperceldid not exhibit lactoperoxidase activity. The highest recoveryof lactoferrin (~91%) with 2.9 fold rise in purity was obtainedwhen the MEP resin column was used with the bindingbuffer without sodium chloride. Thus, MEP HyperCel™could be a potential alternative to existing systems forseparation of lactoferrin from bovine colostrum whey.
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