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Lian-Qun Wang,De-Wu Liu,Wei Lan,Zun-Wen Lin,Pei-Xing Huang 한국조직공학과 재생의학회 2015 조직공학과 재생의학 Vol.12 No.4
Telomerase extends the proliferation and prevent replicative senescence in most somatic cells. Whether it has similar function in human epidermal stem cells remains to be determined. In this study, the human telomerase reverse transcriptase (hTERT) cytalytic subunit was introduced into epidermal stem cells derived from human fetal skins. The expression of hTERT mRNA, protein and telomerase activity in the transduced cells was observed by real time PCR (RT-PCR) techniques, western blot analysis and telomeric repeat amplification protocol enzyme-linked immunosorbent assay (TRAP-ELISA) assay, respectively. The proliferation of the transduced cells was examined. The results showed the introduction of hTERT into the epidermal stem cells upregulated the expression of the hTERT gene and protein. And also the hTERT-transduced epidermal stem cells exhibited significantly elevated telomerase activity and proliferation. These works establish the base of further gene modification, monoclone screening and cell differentiation mechanism. The ability to maintain the biological characteristics and proliferation of epidermal stem cells could have important applications in wound healing and skin tissue engineering.
Fan Dong Kong,Li Man Zhou,Qing Yun Ma,Sheng Zhuo Huang,Pei Wang,Hao Fu Dai,You-Xing Zhao 대한약학회 2017 Archives of Pharmacal Research Vol.40 No.1
Three new compounds named penicitor A,aculene E and penicitor B, as well as four known compounds,were isolated from the fermentation broth ofPenicillium sp. SCS-KFD08 associated with a marineanimal Sipunculus nudus from the Haikou bay of China. Their planar structures and absolute configurations wereunambiguously elucidated by spectroscopic data, Mosher’smethod, CD spectrum analysis along with quantumECD calculation. Among them, compounds 2–7 showedquorum sensing inhibitory activity against Chromobacteriumviolaceum CV026, and could significantly reduceviolacein production in N-hexanoyl-l-homoserine lactone(C6-HSL) induced C. violaceum CV026 cultures at subinhibitoryconcentrations.