Preparation and Release Properties of Electrospun Poly(vinyl alcohol)/ Poly(ε-caprolactone) Hybrid Nanofibers: Optimization of Process Parameters via D-Optimal Design Method

Payam Zahedi,Iraj Rezaeian,Seyed Hassan Jafari,Zeinab Karami 한국고분자학회 2013 Macromolecular Research Vol.21 No.6

The main purpose of this work was to develop biomedical electrospun nanofibrous mats based on a poly(vinyl alcohol)/poly(ε-caprolactone) (80/20) hybrid with a defined drug release rate using tetracycline hydrochloride as a model drug. The electrospinning process parameters, such as polymer solution concentration, distance between injecting syringe tip/collector, voltage, injected flow rate and the polyvinyl alcohol crosslinking time were optimized via a D-optimal design method for a suitable nanofiber diameter with an optimal drug release rate. The morphology of nanofibers and their mean diameters were studied by a scanning electron microscopy technique. The results showed that the mean diameters of nanofibers were significantly reduced after drug loading. The swelling,weight loss and biodegradability of nanofibers samples investigated by FTIR were also determined. Two main mechanisms via penetration and erosion were evaluated. In vitro drug release in a phosphate buffer environment at pH=7.2for the samples demonstrated that the polymer type and hydrophilic nature of the polymer/drug system is very effective in the kinetics and mechanism of drug release. Hybridization of poly(vinyl alcohol)/poly(ε-caprolactone) with a known ratio showed to be a suitable and useful method in the electrospinning of nanofibers samples for superior control of the drug release rate. Finally, nanofibrous mats of polyvinyl alcohol and polyvinyl alcohol/poly(ε-caprolactone)hybrid (80/20) had much better drug release rate characteristics for tetracycline hydrochloride as a model drug compared with cast film samples loaded with the same drug.

Morphological, thermal and drug release studies of poly (methacrylic acid)-based molecularly imprinted polymer nanoparticles immobilized in electrospun poly (ε-caprolactone) nanofibers as dexamethasone delivery system

Payam Zahedi,Mahshid Fallah-Darrehchi,Shima Ahmadi Nadoushan,Robabeh Aeinehvand,Lida Bagheri,Mohammad Najafi 한국화학공학회 2017 Korean Journal of Chemical Engineering Vol.34 No.7

Electrospun poly (ε-caprolactone) (PCL) nanofibers containing molecularly imprinted polymer (MIP) nanoparticles based on methacrylic acid (MAA) were prepared for controlled release of dexamethasone (Dexa). First, the MIPs consisting of Dexa were synthesized via precipitation polymerization. Their recognition sites formation and thermal properties were investigated by FTIR and TGA tests, respectively. The results showed that by selecting a monomer: template (MAA:Dexa) molar ratio of 6 : 1, MIP nanoparticles were produced with imprinting factor of 1.80. The FESEM and TEM images showed the MIPs average diameter of 394±9.7 nm and appropriate immobilization of them in PCL nanofibers, respectively. Moreover, the cumulative release of Dexa from the MIP-loaded nanofibrous samples was studied by UV-vis spectrophotometry and revealed a suitable controlled release of the drug during four days. Afterward, Dexa release followed Higuchi model which indicated the main mechanism was governed by Fickian diffusion theory.

Heparin-coated Poly(ethylene terephthalate)/Graphene Oxide Nanofibers Used for Vascular Tissue Engineering Application

Romina Sepehri,Payam Zahedi,Mahboubeh Kabiri,Chakavak Nojavan 한국섬유공학회 2022 Fibers and polymers Vol.23 No.11

Coating polymeric nanofibers with anti-coagulant agents is one of the most important methods to enhance theperformance of these materials against blood interactions. In this work, poly(ethylene terephthalate) (PET)/graphene oxide(GO) nanofibers coated with heparin (Hep) were fabricated and their physicochemical properties alongside hemocompatibilitywere investigated. To study the effect of GO on PET nanofibers, the electrical conductivity of 4.08×10-8 S cm-1 and elasticmodulus of approximately 47 MPa for sample containing 3 wt.% of GO (PET/GO 3) were observed. After NH3 plasma, Hepwas coated on the optimal PET/GO 3 nanofibrous sample and Fourier transform infrared spectroscopy as well as watercontact angle results exhibited the appropriate Hep coating by dedicating characteristic peak of 1187 cm-1 and 25 °,respectively. In addition, the scanning electron microscopic images of PET/GO 3 nanofibers showed Hep coating has nosignificant influence on the size of nanofibrous diameter. Prothrombin time and partial thromboplastin time were increasedup to 106 s and 350 s; additionally, the number of platelets and hemolytic index were decreased in PET/GO 3 nanofibers inpresence of Hep. Eventually, cultivation of mesenchymal stem cells revealed that the sample coated with Hep dedicated thehighest cell proliferation with the most living cells. The overall outcomes conducted us the Hep-coated PET/GO 3nanofibrous sample was a promising candidate for vascular tissue engineering.

Sodium alginate/xanthan-based nanocomposite hydrogels containing 5-fluorouracil: Characterization and cancer cell death studies in presence of halloysite nanotube

Shadi Zolfagharian,Payam Zahedi,Mehdi Shafiee Ardestani,Alireza Khatibi,Saeed Jafarkhani 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.120 No.-

This work was aimed to synthesize 5-fluorouracil (5-FU)-loaded sodium alginate (SA)/xanthan gum (Xan)hydrogels containing sulfuric acid-treated halloysite nanotube (tHNT) using free radical polymerizationand evaluate their apoptosis against HeLa cells in cervical cancer therapy. The nanocomposite hydrogelincluding tHNT (SA/Xan: tHNT, 1:1) showed a rough surface with cross-linking density of 171.9 10-4mol/cm3 compared to tHNT-free sample (62.5 10-4 mol/cm3). The 5-FU encapsulation efficiency ofthe samples in the presence and absence of tHNT was obtained approximately 60 % and 76 %, respectively. Moreover, the drug release of the samples with and without tHNT were measured 72 % and93 % in acidic medium as well as 32 % and 57 % in basic environment after 72 h. Finally, the cytotoxicityresults revealed the minimum cell viability (3.3 %) for tHNT-loaded hydrogel sample on the 4th day, andflow cytometry assessments also exhibited that the apoptosis rate of HeLa cells for this sample was85.4 %, resulting in its remarkable potential for destroying this cancerous cell line.

Poly(lactic acid) Nanofibrous Scaffolds Containing Aspirin-loaded Zeolitic Imidazolate Frameworks: Morphology, Drug Release, Hemocompatibility and Shape Memory Studies

Fatemeh Salahi Chashmi,Mehrdad Khakbiz,Payam Zahedi,Mahboubeh Kabiri 한국섬유공학회 2022 Fibers and polymers Vol.23 No.8

Using poly(lactic acid) (PLA) as biodegradable and biocompatible material is demanded for vascular scaffoldsespecially a susceptible substitute of permanent metal stents in treatment of coronary heart disease. In this work, PLAnanofibers containing aspirin (Asp) loaded into zeolitic imidazolate framework nanoparticles (ZIF-8@Asp NPs) wereprepared for vascular tissue application. In this line, ZIF-8@Asp NPs were synthesized, and their characterization revealedtheir average NP size and the drug loading efficiency of approximately 180 nm and 76 %. Afterward, ZIF-8@Asp NPs wereloaded into PLA nanofibers and the obtained results showed a uniform morphology with an average nanofibers diameter ofaround 544 nm, Young’s modulus of 1.7 MPa, and the ultimate drug release of 19 % after six days. Besides degradationstudies of the sample, the addition of ZIF-8@Asp NPs to the PLA nanofibers displayed no adverse effect on the shaperecovery properties. Finally, the hemolysis and platelet adhesion confirmed that the surface of the ZIF-8@Asp-loaded PLAnanofibrous samples exhibited appropriate hemocompatibility.

Simulation and in vitro evaluations of microfluidically-fabricated clarithromycin-poly (e-caprolactone) nanoparticles

Beeta Tavana,Alireza Khatibi,Saeed Jafarkhani,Payam Zahedi,Mohammad Hossein Zamani,Seyed Hassan Jafari,Mohammad Najafi 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.124 No.-

Clarithromycin (CLR)-loaded poly (e-caprolactone) (PCL) nanoparticles (NPs) were synthesized usingmicrofluidics (MF) with square-shaped (MF1), and three-channel (MF2), geometries as well as conventionalbulk mixing (BM). Based on Comsol simulation results of MF chips, the flow rate ratio, nonsolventphase flow rate, and polymer solution concentration were optimized 0.05, 50 mL h1, and 0.2(%w/v), respectively; thereby, representing MF1 geometry with highest homogenous mixing. Characterization studies indicated that MF1-based NPs possessed smaller average diameter (91 nm),narrower polydispersity index (0.13) and higher zeta potential (-44 mV) compared to those NPs synthesizedby MF2 and BM methods. Moreover, MF1-aided synthesis of NPs exhibited the encapsulation efficiencyof approximately 81% and loading capacity of around 13%. Investigating CLR release behavior atdifferent pHs also demonstrated that the MF1-based NPs experienced the most controlled release, followedthe Fickian diffusion mechanism. The controlled release of MF1-based NPs was confirmed by evaluatingtheir inhibitory effect against specific cytokines on BEAS-2B as bronchitis cell line via a series ofin vitro experiments including real time-polymerase chain reaction and flow cytometry. Eventually, theantibacterial properties of the samples were studied on two prevalent bacterial strains and the obtainedobservations revealed that MF1-based NPs encompassed the best performance in controlled release ofCLR with respect to the other samples.