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Brice W Obiang-Obounou,강옥화,최장기,Joon-Ho Keum,김성배,Yong-Sik, Kim,문수현,Mi-Sun Choi,Lamidi Maroufath,권동렬 한국생약학회 2011 Natural Product Sciences Vol.17 No.1
The plant species reported here are used by traditional healers in Gabon for different ailments such as wounds, malaria, fever, gonorrhea or diarrhea. The aim of this study was to evaluate the antimicrobial activities of 7 plants (Strombosiopsis tetrandra, Tetraberlinia bifoliolata, Dichapetalum barbatum, Guibourtia demeusii, Dacryodes normandii, Manniophytum fulvum, Paropsia grewoides) against different strains of both Methicillin-Resistant Staphylococcus aureus (MRSA) and Salmonella. Disc diffusion was first used to determine the antimicrobial effectiveness of the plants' ethanolic extracts. Then the minimum inhibitory concentrations of the crude extracts of either leaves or stem barks of the 7 plants were determined using broth micro-dilution. The ethanolic plant extracts showed very good activity against both MRSA and Salmonella strains where the MICs ranged from 250 mg/ml to 1000 mg/ml. The study shows that many of the tested plants used by Gabonese traditional healers have antimicrobial activities and give support to their traditional use.
Anti-Adipogenic Effects on 3T3-L1 Cells and Zebrafish by Tanshinone IIA
Park, Yu-Kyoung,Obiang-Obounou, Brice Wilfried,Lee, Jinho,Lee, Tae-Yun,Bae, Myung-Ae,Hwang, Kyu-Seok,Lee, Kyung-Bok,Choi, Jong-Soon,Jang, Byeong-Churl MDPI 2017 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.18 No.10
<P>Tanshinone IIA is a diterpene quinone isolated from the roots of <I>Salvia miltiorrhiza</I> bunge that has traditionally been used in China for the treatment of cardiovascular and cerebrovascular disorders. Although there is recent evidence showing that tanshinone IIA has an anti-obesity effect, its underlying mechanism of anti-obesity effect is poorly understood. Here, we investigated the effect of tanshinone IIA on lipid accumulation in 3T3-L1 preadipocytes and zebrafish. Notably, tanshinone IIA at 10 μM concentration greatly reduced lipid accumulation and triglyceride (TG) contents during 3T3-L1 preadipocyte differentiation, suggesting its anti-adipogenic effect. On mechanistic levels, tanshinone IIA reduced the expression levels of CCAAT/enhancer-binding protein-α (C/EBP-α), peroxisome proliferator-activated receptor-γ (PPAR-γ), fatty acid synthase (FAS), and perilipin A but also the phosphorylation levels of signal transducer and activator of transcription-3/5 (STAT-3/5) in differentiating 3T3-L1 cells. In addition, tanshinone IIA strongly inhibited leptin and resistin mRNA expression in differentiating 3T3-L1 cells. Importantly, the tanshinone IIA’s lipid-reducing effect was also seen in zebrafish. In sum, these findings demonstrate that tanshinone IIA has anti-adipogenic effects on 3T3-L1 cells and zebrafish, and its anti-adipogenic effect on 3T3-L1 cells is largely attributable to the reduced expression and/or phosphorylation levels of C/EBP-α, PPAR-γ, FAS, perilipin A, and STAT-3/5.</P>
Naik, Ravi,Obiang-Obounou, Brice W,Kim, Minkyoung,Choi, Yongseok,Lee, Hyun Sun,Lee, Kyeong Wiley-VCH 2014 CHEMMEDCHEM Vol.9 No.11
<P>Metabolic diseases such as atherogenic dyslipidemia, hepatic steatosis, obesity, and type II diabetes are emerging as major global health problems. Acyl-CoA:diacylglycerol acyltransferase (DGAT) is responsible for catalyzing the final reaction in the glycerol phosphate pathway of triglycerol synthesis. It has two isoforms, DGAT-1 and DGAT-2, which are widely expressed and present in white adipose tissue. DGAT-1 is most highly expressed in the small intestine, whereas DGAT-2 is primarily expressed in the liver. Therefore, the selective inhibition of DGAT-1 has become an attractive target with growing potential for the treatment of obesity and type II diabetes. Furthermore, DGAT-2 has been suggested as a new target for the treatment of DGAT-2-related liver diseases including hepatic steatosis, hepatic injury, and fibrosis. In view the discovery of drugs that target DGAT, herein we attempt to provide insight into the scope and further reasons for optimization of DGAT inhibitors.</P>
Park, Yu‐,Kyoung,Obiang‐,Obounou, Brice Wilfried,Lee, Kyung‐,Bok,Choi, Jong‐,Soon,Jang, Byeong‐,Churl John Wiley and Sons Inc. 2018 JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Vol.22 No.4
<P><B>Abstract</B></P><P>The proviral integration moloney murine leukaemia virus (Pim) kinases, consisting of Pim‐1, Pim‐2 and Pim‐3, are involved in the control of cell growth, metabolism and differentiation. Pim kinases are emerging as important mediators of adipocyte differentiation. AZD1208 is a pan‐Pim kinase inhibitor and is known for its anti‐cancer activity. In this study, we investigated the effect of AZD1208 on adipogenesis and lipolysis in 3T3‐L1 cells, a murine preadipocyte cell line. AZD1208 markedly suppressed lipid accumulation and reduced triglyceride contents in differentiating 3T3‐L1 cells, suggesting the drug's anti‐adipogenic effect. On mechanistic levels, AZD1208 reduced not only the expressions of CCAAT/enhancer‐binding protein‐α (C/EBP‐α), peroxisome proliferator‐activated receptor‐γ (PPAR‐γ), fatty acid synthase (FAS), acetyl‐CoA carboxylase (ACC) and perilipin A but also the phosphorylation of signal transducer and activator of transcription‐3 (STAT‐3) in differentiating 3T3‐L1 cells. Remarkably, AZD1208 increased cAMP‐activated protein kinase (AMPK) and LKB‐1 phosphorylation while decreased intracellular ATP contents in differentiating 3T3‐L1 cells. Furthermore, in differentiated 3T3‐L1 adipocytes, AZD1208 also partially promoted lipolysis and enhanced the phosphorylation of hormone‐sensitive lipase (HSL), a key lipolytic enzyme, indicating the drug's HSL‐dependent lipolysis. In summary, the findings show that AZD1208 has anti‐adipogenic and lipolytic effects on 3T3‐L1 adipocytes. These effects are mediated by the expression and/or phosphorylation levels of C/EBP‐α, PPAR‐γ, FAS, ACC, perilipin A, STAT‐3, AMPK and HSL.</P>
Ji-Hyun Bae,Brice Wilfried Obiang Obounou 한국식품영양과학회 2018 Preventive Nutrition and Food Science Vol.23 No.2
Dental caries remains one of the most common chronic diseases affecting children worldwide with a multifactorial etiology. The objective of the study was to evaluate the association between socioeconomic status (SES), dietary intake, food insecurity (FI), and dental caries in Korean children and adolescents. The study utilized data from the 2-year Korean National Health and Nutrition Examination Survey (KNHANES) conducted with 1,559 Korean boys and 1,391 girls aged 2 to 18 years from 2012 to 2013. Fathers’ education (P=0.017), mothers’ education (P<0.001), and household income (P=0.049) were all significantly associated with dental caries among Korean boys. As for dietary practices, both eating breakfast (P<0.001) and frequency of eating out (P<0.001) were strongly associated with dental caries (P<0.001). Three models of FI were used and no differences were found regarding genders. In model 3, both food insecure male [odds ratio (OR)=1.682, 95% confidence interval (CI): 0.999∼2.832] and female (OR=1.900, 95% CI: 1.094∼3.299) subjects had higher odds of developing dental caries than food secure subjects after adjusting the confounding factors. The present study showed a strong association between FI mediated by SES and dental caries. Nutrition education programs targeting low-socioeconomic families are necessary as a tool to prevent dental caries in Korea.
Tetrahydropalmatine Inhibits Pro-Inflammatory Mediators in Lipopolysaccharide-Stimulated THP-1
You-Chang Oh,최장기,Young-Seob Lee,Obiang-Obounou Brice,Soo-Chan Lee,Hwa-Sun Kwak,Young-Ho Byun,Ok-Hwa Kang,노정래,Dong-Won Shin,권동렬 한국식품영양과학회 2010 Journal of medicinal food Vol.13 No.5
For centuries, Corydalis Rhizoma has been used in Korean traditional medicine. Tetrahydropalmatine is an alkaloid compound and a prominent anti-inflammatory agent found in plants, including Corydalis yanhusuo. However, the therapeutic mechanisms of tetrahydropalmatine are still not well understood. To provide insight into the biological effects of tetrahydropalmatine, we examined its influence on lipopolysaccharide (LPS)-induced interleukin (IL)-8 production in the human monocytic cell line THP-1. In the present study, IL-8 production was measured using enzyme-linked immunosorbent assay, reverse transcription-polymerase chain reaction, and mitogen-activated protein kinase (MAPK) activation, as determined by western blot analysis. Tetrahydropalmatine inhibited LPS-induced IL-8 production in a dose-dependent manner. Furthermore, tetrahydropalmatine inhibited extracellular signal-regulated kinase and p38 MAPK phosphorylation, which suggests that tetrahydropalmatine inhibits IL-8 secretion by blocking MAPK phosphorylation. Taken together, these findings may help elucidate the mechanism by which tetrahydropalmatine modulates THP-1 cell activation under inflammatory conditions
Oh, You-Chang,Kang, Ok-Hwa,Choi, Jang-Gi,Brice, Obiang-Obounou,Lee, Young-Seob,Keum, Joon-Ho,Kim, Sung-Bae,Shin, Dong-Won,Ma, JinYeul,Jeong, Gi-Hoon,Kwon, Dong-Yeul Informa Healthcare 2011 Immunopharmacology and immunotoxicology Vol.33 No.2
<P>Sinomenine is an alkaloid compound and a prominent anti-allergic agent found in the root of the climbing plant <I>Sinomenium acutum</I>. However, its effects on the bone marrow-derived mast cell (BMMC) mediated allergy and inflammation mechanism remain unknown. In this study, the biological effects of sinomenine were evaluated while focusing on its effects on the allergic mediator in PMA plus A23187-stimulated BMMCs. An investigation was also conducted to determine its effects on the production of several allergic mediators including interleukin-6 (IL-6), prostaglandin D<SUB>2</SUB> (PGD<SUB>2</SUB>), leukotriene C<SUB>4</SUB> (LTC<SUB>4</SUB>), β棺-Hexosaminidase (β棺-Hex), and cyclooxygenase-2 (COX-2) protein. The results revealed that sinomenine inhibited the PMA plus A23187-induced production of IL-6, PGD<SUB>2</SUB>, LTC<SUB>4</SUB>, β棺-Hex, and COX-2 protein. Taken together, these findings indicate that sinomenine has the potential for use in the treatment of allergy.</P>