Production of Recombinant Humanized Anti-HBsAg Fab Fragment from Pichia pastoris by Fermentation

Ning Deng,Jun Jian Xiang,Qing Zhang,Sheng Xiong,Wen Yin Chen,Gui Rong Rao,Xun Zhang Wang 한국생활환경학회 2005 BMB Reports Vol.38 No.3

Screening of the Antigen Epitopes of Basic Fibroblast GrowthFactor by Phage Display

Deng Ning,Xiang Junjian,Zhong Zhenyu,Zhong Zhendong,Yang Hongyu 한국생화학분자생물학회 2005 BMB Reports Vol.38 No.3

Production of Recombinant Humanized Anti-HBsAg Fab Fragment from Pichia pastoris by Fermentation

Deng, Ning,Xiang, Junjian,Zhang, Qing,Xiong, Sheng,Chen, Wenyin,Rao, Guirong,Wang, Xunzhang Korean Society for Biochemistry and Molecular Biol 2005 Journal of biochemistry and molecular biology Vol.38 No.3

In this report, we describe the high-yield secretory expression of the recombinant human anti-HBsAg Fab fragment from Pichia pastoris that was achieved by co-integration of the genes encoding the heavy and light chains (both under the control of alcohol oxidase promoter) into the genome of the yeast cells. The fed-batch fermentations were carried out in a 5 L scale. Both chains of the Fab were successfully expressed upon methanol induction. The absorbance ($OD_{600}$) of the broth can reach 350~500 at the end of fed-batch phase. After the induction, the expression level of the recombinant Fab (soluble) reached 420~458 mg/L. The recombinant Fab fragment was purified from the crude culture supernatant by ion exchange chromatography and the purity of the recombinant Fab fragment was over 95%. The affinity activities of the crude fermentation supernatant and the purified Fab were analyzed by indirect ELISA, which showed that the purified recombinant Fab fragment had high affinity activity with hepatitis B surface antigen.

Optical and structural investigation of stacked Ge quantum dots

Ning Deng,Peiyi Chen,Rongshan Wei,Zhijian Li 한국물리학회 2004 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.45 No.3

Stacked Ge quantum dots were grown on Si(100) by ultra-high vacuum chemical vapor deposition( UHV/CVD). The morphology and the size distributions of stacked Ge dots were investigated by AFM and TEM. The in uences of the number of layers and thickness of Si spacer layers on upper Ge dots were investigated. Results show that the total density of upper Ge dots decreases with increasing of the number of layers. To obtain vertical correlated Ge dots, the thickness of Si spacer layers should be smaller than 48nm. Obvious blueshift (87meV) observed from PL spectrum under 10K demonstrates strong quantum connement in Ge dots. FWHM of Ge dots NP peak is about 46meV that indicates the narrow size distribution of stacked Ge dots grown by UHV/CVD. Finally, a photodetector using stacked Ge quantum dots as active region was demonstrated as well.

Preoperative body mass index and amylase level predict postoperative pancreatic fistula independently

Deng NING,Li JIANG,Xiaoping CHEN,Chao WANG 한국간담췌외과학회 2021 Annals of hepato-biliary-pancreatic surgery Vol.25 No.-

Network Analysis of microRNAs, Genes and their Regulation in Mantle Cell Lymphoma

Deng, Si-Yu,Guo, Xiao-Xin,Wang, Ning,Wang, Kun-Hao,Wang, Shang Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.2

The pathogenesis of mantle cell lymphoma, a special subtype of lymphoma that is invasive and indolent and has a median survival of 3 to 4 years, is still partially unexplained. Much research about genes and miRNAs has been conducted in recent years, but interactions and regulatory relations of genetic elements which may play a vital role in genesis of MCL have attracted only limited attention. The present study concentrated on regulatory relations about genes and miRNAs contributing to MCL pathogenesis. Numerous experimentally validated raw data were organized into three topology networks, comprising differentially expressed, associated and global examples. Comparison of similarities and dissimilarities of the three regulating networks, paired with the analysis of the interactions between pairs of elements in every network, revealed that the differentially expressed network illuminated the carcinogenicity mechanism of MCL and the related network further described the regulatory relations involved, including prevention, diagnosis, development and therapy. Three kinds of regulatory relations for host genes including miRNAs, miRNAs targeting genes and genes regulating miRNAs were concluded macroscopically. Regulation of the differentially expressed miRNAs was also analyzed, in terms of abnormal gene expression affecting the MCL pathogenesis. Special regulatory relations were uncovered. For example, auto-regulatory loops were found in the three topology networks, key pathways of the nodes being highlighted. The present study focused on a novel point of view revealing important influencing factors for MCL pathogenesis.

Structural features and thermoelectric performance of chalcopyrite Cu(In, Ga)Te2 system by isoelectronic substitution

Deng Shuping,Jiang Xianyan,Zhang Ziye,Liu Junjie,Chen Lili,Qi Ning,Tang Xinfeng,Wu Yichu,Chen Zhiquan 한국물리학회 2021 Current Applied Physics Vol.26 No.-

In this paper, a series of CuIn1-xGaxTe2 samples were prepared by vacuum melting combined with the spark plasma sintering process based on the initial stoichiometric ratios of 1: 1-x: x: 2 (x = 0, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9 and 1.0). Crystal results indicate that all Cu(In, Ga)Te2 system samples are the chalcopyrite structure with space group I42d. Thermal analysis results show that all the samples have excellent reproducibility and thermal stability. EPMA data indicates that Ga tended to replace In site instead of Cu or Te. Meanwhile, the roomtemperature carrier concentration of all p-type samples varies from 0.30 × 1019 to 1.25 × 1019cm 3, since carrier mobility changes from 15.16 to 69.27 cm2 V 1 s 1. Ultimately, the significantly reduced total thermal conductivity is observed in the Ga-doped samples, and the maximum ZT value of 0.80 is obtained at 773 K for the CuIn0.8Ga0.2Te2 sample due to the lower thermal conductivity

Clinical Observation and Therapeutic Evaluation of Rh-endostatin Combined with DP Regimen in Treating Patients with Advanced Esophageal Cancer

Deng, Wen-Ying,Song, Tao,Li, Ning,Luo, Su-Xia,Li, Xiang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.16

Objective: To observe the curative effects of rh-endostatin combined with DP regimen in treating patients with advanced esophageal cancer and analyze the correlation of CT perfusion (CTP) parameters and the expression of vascular endothelial growth factor (VEGF). Methods: Twenty patients with esophageal cancer confirmed pathologically were randomly divided into combined treatment (rh-endostatin+DP regimen) group and single chemotherapy group, 10 patients in each group, respectively. All patients were given conventional CT examination and CTP imaging for primary tumor. The level of VEGF, the size of tumor and CTP parameters (BF, BV, PS and MTT) before treatment and after 2 cycles of treatment were determined for the comparison and the correlation between CTP parameters and VEGF expression was analyzed. Results: the therapeutic effect of rh-endostatin+DP regimen group was superior to single chemotherapy group. VEGF level after treatment in rh-endostatin+DP regimen group was obviously lower than single chemotherapy group (P<0.01). The expression of VEGF had positive correlation with BF and BV but negative correlation with MTT. Compared with treatment before for rh-endostatin+DP regimen group, BF, BV and PS decreased while MTT increased after treatment (P<0.05). However, there were no significant differences between treatment before and after treatment in single chemotherapy (P>0.05). Conclusions: Rh-endostatin can down-regulate the expression of VEGF in esophageal cancer, change the state of hypertransfusion and high permeability of tumor vessels and had the better curative effect and slighter adverse reactions when combined with chemotherapy.

Ultrasound-Guided Radiofrequency Ablation in Tertiary Hyperparathyroidism: A Prospective Study

Deng Erya,Jiang Tingting,Chai Huihui,Weng Ning,He Hongfeng,Zhang Zhengxian,Peng Chengzhong,Yue Wenwen,Xu Huixiong 대한영상의학회 2024 Korean Journal of Radiology Vol.25 No.3

Objective: To prospectively evaluate the outcomes of ultrasound (US)-guided radiofrequency ablation (RFA) in tertiary hyperparathyroidism (THPT). Materials and Methods: Patients with THPT underwent RFA between September 2017 and January 2022. Laboratory parameters, including serum intact parathyroid hormone (iPTH) levels, were monitored for 48 months after RFA and compared with the levels at baseline. Complications related to RFA and changes in hyperparathyroidism-related clinical symptoms were recorded before and after RFA. Results: A total of 42 patients with THPT were recruited for this study. Ultimately, 36 patients with renal failure and 2 patients who underwent successful renal transplantation (male:female, 17:21; median age, 54.5 years) were enrolled. The follow-up time was 21.5 ± 19.0 months in the 36 patients with renal failure. In these 36 patients, iPTH levels were significantly decreased to 261.1 pg/mL at 48 months compared with the baseline value of 1284.9 pg/mL (P = 0.012). Persistent hyperparathyroidism, defined as iPTH levels maintained at > 585.0 pg/mL for 6 months after treatment, occurred in 4.0% of patients (1/25). Recurrent hyperparathyroidism, defined as iPTH levels > 585.0 pg/mL after 6 months, were 4.0% (1/25) and 0.0% (0/9) at 6 months and 4 years after treatment, respectively. In two patients with THPT after successful renal transplantation, iPTH decreased from the baseline value of 242.5 and 115.9 pg/mL to 171.0 and 62.0 pg/mL at 6 months after treatment. All complications resolved within 6 months of ablation without medical intervention, except in 10.5% (4/38) patients with permanent hypocalcemia. The overall symptom recovery rate was 58.8% (10/17). The severity scores for bone pain, arthralgia, and itchy skin associated with hyperparathyroidism improved after treatment (P < 0.05). Conclusion: US-guided RFA is an effective and safe alternative to surgery in the treatment of patients with TPTH and improves hyperparathyroidism-related clinical symptoms.

The bifunctional effect of propofol on thromboxane agonist (U46619)-induced vasoconstriction in isolated human pulmonary artery

Ning Hao,Zhaojun Wang,Sujuan Kuang,Guangyan Zhang,Chunyu Deng,Jue Ma,Jianxiu Cui 대한생리학회-대한약리학회 2017 The Korean Journal of Physiology & Pharmacology Vol.13 No.3

Propofol is known to cause vasorelaxation of several systemic vascular beds. However, its effect on the pulmonary vasculature remains controversial. In the present study, we investigated the effects of propofol on human pulmonary arteries obtained from patients who had undergone surgery. Arterial rings were mounted in a Multi-Myograph system for measurement of isometric forces. U46619 was used to induce sustained contraction of the intrapulmonary arteries, and propofol was then applied (in increments from 10-300 μM). Arteries denuded of endothelium, preincubated or not with indomethacin, were used to investigate the effects of propofol on isolated arteries. Propofol exhibited a bifunctional effect on isolated human pulmonary arteries contracted by U46619, evoking constriction at low concentrations (10-100 μM) followed by secondary relaxation (at 100-300 μM). The extent of constriction induced by propofol was higher in an endothelium-denuded group than in an endothelium-intact group. Preincubation with indomethacin abolished constriction and potentiated relaxation. The maximal relaxation was greater in the endothelium-intact than the endothelium-denuded group. Propofol also suppressed CaCl₂-induced constriction in the 60 mM K⁺-containing Ca²⁺-free solution in a dose-dependent manner. Fluorescent imaging of Ca²⁺ using fluo-4 showed that a 10 min incubation with propofol (10-300 μM) inhibited the Ca²⁺ influx into human pulmonary arterial smooth muscle cells induced by a 60 mM K⁺-containing Ca²⁺-free solution. In conclusion, propofol-induced arterial constriction appears to involve prostaglandin production by cyclooxygenase in pulmonary artery smooth muscle cells and the relaxation depends in part on endothelial function, principally on the inhibition of calcium influx through L-type voltage-operated calcium channels.