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Mehta Nikhil,Rao Priyanka,Saini Raman 한국작물학회 2021 Journal of crop science and biotechnology Vol.24 No.5
Sesbania aculeata is an important legume crop used in agriculture, industries, as food, and traditional medicines. For its further genetic improvement, particularly to overcome the anti-nutritional factors of the seeds requires biotechnological approaches. In the line, the present work reports frst-time optimization of various parameters (plant growth regulators, orientation and polarity of explants, preconditioning of the seedlings, presence of silver nitrate, and vacuum infltration) afecting in vitro regeneration and transformation. To raise the transformant of Sesbania aculeata, the study used hypocotyl explants and plasmid pCAMBIA2301 immobilized in Agrobacterium tumefaciens strain EHA105. The 3-day-old hypocotyl explants were obtained from pre-conditioned seedlings and cultured horizontally on Murashige and Skoog (MS) salts and Gamborg (B5) vitamins containing medium augmented with 2.5 µM 6-benzylaminopurine and 1.0 mg/L silver nitrate and produced multiple shoots (11–12) in 100% cultures within 28 days. The explants were vacuum infltrated at 660 mm of Hg for 5 min in presence of A. tumefaciens and cultured on shoot regeneration medium containing kanamycin 80 mg/L to regenerate shoots. To select at the rooting level the regenerants were rooted in presence of 2.5 µM indole-3-butyric acid and 10 mg/L kanamycin. Validate the transgenics for the transgene’s presence, integration, expression and inheritance up to the progeny plants via PCR, RT-PCR, Southern analysis, and histochemical GUS assay. The stable histochemical GUS expression was observed in various parts of primary transformants and progeny (T1) seedlings. Overall transformation efciency of 11.33% was achieved in 6–8 weeks.
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Peroral Endoscopic Myotomy: Establishing a New Program
Nikhil A. Kumta,Shivani Mehta,Prashant Kedia,Kristen Weaver,Reem Z. Sharaiha,Norio Fukami,Hitomi Minami,Fernando Casas,Monica Gaidhane,Arnon Lambroza,Michel Kahaleh 대한소화기내시경학회 2014 Clinical Endoscopy Vol.47 No.5
Achalasia is an esophageal motility disorder characterized by incomplete relaxation of the lower esophageal sphincter (LES) and aperistalsis of the esophageal body. Treatment of achalasia is aimed at decreasing the resting pressure in the LES. Peroral endoscopic myotomy (POEM), derived from natural orifice transluminal endoscopic surgery (NOTES) and advances in endoscopic submucosal dissection (ESD), presents a novel, minimally invasive, and curative endoscopic treatment for achalasia. POEM involves an esophageal mucosal incision followed by creation of a submucosal tunnel crossing the esophagogastric junction and myotomy before closure of the mucosal incision. Although the procedure is technically demanding and requires a certain degree of skill and competency, treatment success is high (90%) with low complication rates. Since the first described POEM in humans in 2010, it has been used increasingly at centers worldwide. This article reviews available published clinical studies demonstrating POEM efficacy and safety in order to present a proposal on how to establish a dedicated POEM program and reach base proficiency for the procedure.
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Internet search analytics for shoulder arthroplasty: what questions are patients asking?
Johnathon R. McCormick,Matthew C. Kruchten,Nabil Mehta,Dhanur Damodar,Nolan S. Horner,Kyle D. Carey,Gregory P. Nicholson,Nikhil N. Verma,Grant E Garrigues 대한견주관절학회 2023 대한견주관절의학회지 Vol.26 No.1
Background: Common questions about shoulder arthroplasty (SA) searched online by patients and the quality of this content are unknown. The purpose of this study is to uncover questions SA patients search online and determine types and quality of webpages encountered. Methods: The “People also ask” section of Google Search was queried to return 900 questions and associated webpages for general, anatomic, and reverse SA. Questions and webpages were categorized using the Rothwell classification of questions and assessed for quality using the Journal of the American Medical Association (JAMA) benchmark criteria. Results: According to Rothwell classification, the composition of questions was fact (54.0%), value (24.7%), and policy (21.3%). The most common webpage categories were medical practice (24.6%), academic (23.2%), and medical information sites (14.4%). Journal articles represented 8.9% of results. The average JAMA score for all webpages was 1.69. Journals had the highest average JAMA score (3.91), while medical practice sites had the lowest (0.89). The most common question was, “How long does it take to recover from shoulder replacement?” Conclusions: The most common questions SA patients ask online involve specific postoperative activities and the timeline of recovery. Most information is from low-quality, non-peer-reviewed websites, highlighting the need for improvement in online resources. By understanding the questions patients are asking online, surgeons can tailor preoperative education to common patient concerns and improve postoperative outcomes.
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Nishant Kumar,Karen Xie,Winnie Mar,Thomas M. Anderson,Benjamin Carney,Nikhil Mehta,Roberto Machado,Michael J. Blend,Yang Lu 대한핵의학회 2015 핵의학 분자영상 Vol.49 No.4
Purpose To investigate the diagnostic performance of perfusion single-photon emission computed tomography/computed tomography (Q-SPECT/CT) in patients suspected to have pulmonary embolism (PE) but with indeterminate computed tomographic pulmonary angiography (CTPA) or planar ventilation/perfusion (V/Q) scans. Methods This retrospective study included two groups of patients. Group I consisted of 49 patients with nondiagnostic CTPA. These 49 patients underwent subsequent V/Q scans. Further Q-SPECTs were obtained in patients with indeterminate planar images and fused with existing CTPA. Group II consisted of 182 non-CTPA patients with indeterminate V/Q scans. These 182 patients underwent further Q-SPECT and separate noncontrast low-dose CT chest. Fusion Q-SPECT/ CT scans were obtained through FDA-approved software and interpreted according to published criteria as positive, negative, or indeterminate for PE. Upon retrospective analyses, the final diagnosis was made using composite reference standards including all available clinical and imaging information for at least 6-month follow-up. Results In group I patients, 1 was positive, 24 were negative, and another 24 (49 %, 24/49) were indeterminate. In the subsequent 24 Q-SPECT/CTPAs, 4 were positive, 19 were negative, and 1 was indeterminate (4.2 %, 1/24). In group II patients, 9 (4.9 %, 9/182) were indeterminate, 33 were positive, and 140 were negative. The combined nondiagnostic rate for Q-SPECT/CT was only 4.9 % (10/ 206). There was six false-negative and one false-positive Q-SPECT/CT examinations. The sensitivity, specificity, and positive and negative predictive value of Q-SPECT/ CT were 85.7 % (36/42), 99.4 % (153/154), 97.3 % (36/ 37) and 96.2 % (153/159), respectively. Conclusions Q-SPECT/CT improves the diagnostic rate with promising accuracy in diagnosing PE that yields a satisfactory clinical verdict, especially when the CTPA and planar V/Q scan are indeterminate.
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