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Screening and Production of Manganese Peroxidase from Fusarium sp. on Residue Materials
( Nguyen Duc Huy ),( Nguyen Thi Thanh Tien ),( Le Thi Huyen ),( Hoang Tan Quang ),( Truong Quy Tung ),( Nguyen Ngoc Luong ),( Seung-moon Park ) 한국균학회 2017 Mycobiology Vol.45 No.1
In this study, we report the manganese peroxidase production ability from a Fusarium sp. strain using an inexpensive medium of agriculture residues of either rice straw or wood chips as carbon source. The highest manganese peroxidase activity on rice straw medium and on wood chips was 1.76 U/mL by day 9 and 1.91 U/mL by day 12, respectively.
Overexpression of ginseng UGT72AL1 causes organ fusion in the axillary leaf branch of Arabidopsis
Ngoc Quy Nguyen,이옥란 고려인삼학회 2017 Journal of Ginseng Research Vol.41 No.3
Background: Glycosylation of natural compounds increases the diversity of secondary metabolites. Glycosylation steps are implicated not only in plant growth and development, but also in plant defense responses. Although the activities of uridine-dependent glycosyltransferases (UGTs) have long been recognized, and genes encoding them in several higher plants have been identified, the specific functions of UGTs in planta remain largely unknown. Methods: Spatial and temporal patterns of gene expression were analyzed by quantitative reverse transcription (qRT)-polymerase chain reaction (PCR) and GUS histochemical assay. In planta transformation in heterologous Arabidopsis was generated by floral dipping using Agrobacterium tumefaciens (C58C1). Protein localization was analyzed by confocal microscopy via fluorescent protein tagging. Results: PgUGT72AL1 was highly expressed in the rhizome, upper root, and youngest leaf compared with the other organs. GUS staining of the promoter: GUS fusion revealed high expression in different organs, including axillary leaf branch. Overexpression of PgUGT72AL1 resulted in a fused organ in the axillary leaf branch. Conclusion: PgUGT72AL1, which is phylogenetically close to PgUGT71A27, is involved in the production of ginsenoside compound K. Considering that compound K is not reported in raw ginseng material, further characterization of this gene may shed light on the biological function of ginsenosides in ginseng plant growth and development. The organ fusion phenotype could be caused by the defective growth of cells in the boundary region, commonly regulated by phytohormones such as auxins or brassinosteroids, and requires further analysis.
Ngoc Quy Nguyen,이상춘,양태진,이옥란 고려인삼학회 2017 Journal of Ginseng Research Vol.41 No.3
Background: Prenyltransferases catalyze the sequential addition of isopentenyl diphosphate units to allylic prenyl diphosphate acceptors and are classified as either trans-prenyltransferases (TPTs) or cisprenyltransferases (CPTs). The functions of CPTs have been well characterized in bacteria, yeast, and mammals compared to plants. The characterization of CPTs also has been less studied than TPTs. In the present study, molecular cloning and functional characterization of a CPT from a medicinal plant, Panax ginseng Mayer were addressed. Methods: Gene expression patterns of PgCPT1 were analyzed by quantitative reverse transcription polymerase chain reaction. In planta transformation was generated by floral dipping using Agrobacterium tumefaciens. Yeast transformation was performed by lithium acetate and heat-shock for rer2D complementation and yeast-two-hybrid assay. Results: The ginseng genome contains at least one family of three putative CPT genes. PgCPT1 is expressed in all organs, but more predominantly in the leaves. Overexpression of PgCPT1 did not show any plant growth defect, and its protein can complement yeast mutant rer2D via possible protein eprotein interaction with PgCPTL2. Conclusion: Partial complementation of the yeast dolichol biosynthesis mutant rer2D suggested that PgCPT1 is involved in dolichol biosynthesis. Direct protein interaction between PgCPT1 and a human Nogo-B receptor homolog suggests that PgCPT1 requires an accessory component for proper function.
인삼 유래 UDP-의존적인 Glycosyltransferase 유전자의 식물 생장에 미치는 영향 및 환경변화에 대한 반응
Ngoc Quy Nguyen,Su Ji Joo,Ok Ran Lee 한국약용작물학회 2016 한국약용작물학술대회 발표집 Vol.2016 No.10
Background : Glycosylation of natural compounds results in great diversity of secondary metabolites. Glycosylation steps are implicated not only in plants growth and development but also in plant defense responses to various environmental stresses. This process is mediated by members of a multigene superfamily glycosyltransferase (GT), which catalyze the transfer of single or multiple activated sugars to a wide range of substrates, thus influences their chemical property and bioactivity. Although its activity has been recognized for a long time and genes coding UGTs in several higher plants have been identified, specific function of GTs in detail still remains elusive. Methods and Results : Spatial and temporal expression patterns of a ginseng UDP-dependent glycosyltransferase, was analyzed by qRT-PCR. It was expressed highly in rhizome, upper root and youngest leaf compared that of other organs. Spacial expression was observed by GUS histochemical assay after generating promoter::GUS fusion. Noticeably, it expressed axillary branch as well as other organs tested by qRT-PCR. Overexpression of PgUGT in Arabidopsis resulted in fused organ in axillary branch. Stress responsiveness against various abiotic stresses and subcellular localization in Arabidopsis are also addressed. Conclusion : PgUGT phylogenetically closed to PgUGT71A27 involved in ginsenoside compound K (C-K) production. Considering that the C-K is not reported in raw ginseng material, further characterization of this gene may shed light on the biological function of C-K in ginseng growth and development. Organ fusion phenotype could be caused by defective growth of cells in boundary region, commonly regulated by phytohormones such as auxins or brassinosteroids, which in needs to be analyzed further.
인삼에서 cis-Prenyltransferase 유전자는 Nogo-B Receptor 상동유전자와 물리적 결합으로 Dolichol 생합성에 작용
Ngoc Quy Nguyen,Jin Hoon Jang,Ok Ran Lee 한국약용작물학회 2016 한국약용작물학술대회 발표집 Vol.2016 No.10
Background : Prenyltransferases catalyze the sequential addition of IPP units to allylic prenyl diphosphate acceptors and are classified as either trans-prenyltransferases (TPTs) or cis-prenyltransferases (CPTs). Although CPTs and TPTs share similar substrate preferences and reaction products, they can be easily distinguished by their primary amino acid sequences. The characterization of cis-prenyltransferases has been less studied than that of trans-prenyltransferases. Methods and Results : Gene expression patterns of PgCPT1 was analyzed by qRT-PCR. In planta transformation was generated by floral dipping using Agrobacterium tumefaciens. Yeast transformation was performed by lithium acetate and heat-shock for rer2Δ complementation and yeast-two-hybrid assay. Ginseng genome contains at least one family of three putative CPT genes. PgCPT1 is expressed in all organs, but more predominantly in the leaves. Overexpression of PgCPT1 did not show any plant growth defect, and can complement yeast mutant rer2Δ via possible protein-protein interaction with PgCPTL2. Conclusion : Partial complementation of the yeast dolichol biosynthesis mutant rer2Δ suggested that PgCPT1 is involved in some of dolichol biosynthesis. Direct protein interaction between PgCPT1 and a human Nogo-B receptor homolog suggests that PgCPT1 requires an accessory component for proper function.
Nguyen, Ngoc Quy,Lee, Sang-Choon,Yang, Tae-Jin,Lee, Ok Ran The Korean Society of Ginseng 2017 Journal of Ginseng Research Vol.41 No.3
Background: Prenyltransferases catalyze the sequential addition of isopentenyl diphosphate units to allylic prenyl diphosphate acceptors and are classified as either trans-prenyltransferases (TPTs) or cis-prenyltransferases (CPTs). The functions of CPTs have been well characterized in bacteria, yeast, and mammals compared to plants. The characterization of CPTs also has been less studied than TPTs. In the present study, molecular cloning and functional characterization of a CPT from a medicinal plant, Panax ginseng Mayer were addressed. Methods: Gene expression patterns of PgCPT1 were analyzed by quantitative reverse transcription polymerase chain reaction. In planta transformation was generated by floral dipping using Agrobacterium tumefaciens. Yeast transformation was performed by lithium acetate and heat-shock for $rer2{\Delta}$ complementation and yeast-two-hybrid assay. Results: The ginseng genome contains at least one family of three putative CPT genes. PgCPT1 is expressed in all organs, but more predominantly in the leaves. Overexpression of PgCPT1 did not show any plant growth defect, and its protein can complement yeast mutant $rer2{\Delta}$ via possible protein-protein interaction with PgCPTL2. Conclusion: Partial complementation of the yeast dolichol biosynthesis mutant $rer2{\Delta}$ suggested that PgCPT1 is involved in dolichol biosynthesis. Direct protein interaction between PgCPT1 and a human Nogo-B receptor homolog suggests that PgCPT1 requires an accessory component for proper function.