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Research Articles : Trichoderma asperellum Chi42 Genes Encode Chitinase
( Nguyen Hoang Loc ),( Hoang Tan Quang ),( Nguyen Bao Hung ),( Nguyen Duc Huy ),( Truong Thi Bich Phuong ),( Tran Thi Thu Ha ) 한국균학회 2011 韓國菌學會誌 Vol.39 No.3
Four Trichoderma strains (CH2, SH16, PQ34, and TN42) were isolated from soil samples collected from Quang Tri and Thua Thien Hue provinces in Vietnam. The strains exhibited high chitinolytic secretion. Strain PQ34 formed the largest zone of chitinase-mediated clearance (> 4 cm in diameter) in agar containing 1% (w/v) colloidal chitin. Analysis of the internal transcribed spacer regions of these strains indicated that they were Trichoderma asperellum. The molecular weights of the chitinases were approximately 42 kDa. Chitinase genes (chi42) of T. asperellum strains TN42, CH2, SH16, and PQ34 were 98~99% homologous to the ech42 gene of T. harzianum CB-Pin-01 (accession No. DQ166036). The deduced amino acid sequences of both T. asperellum strains SH16 and TN42 shared 100% similarity.
Nguyen Hoang Loc,Truong Thi Phuong Lan,Nguyen Duc Huy,Nguyen Ngoc Luong,Hoang Tan Quang,Trinh Huu Tan,Le Thi Anh Thu,Nguyen Xuan Huy 한국식물생명공학회 2019 JOURNAL OF PLANT BIOTECHNOLOGY Vol.46 No.3
The aim of this study is to evaluate the effect of yeast extract (YE) and salicylic acid (SA) on the expression of curcuminoid-biosynthesis genes (CzDCS and CURS1-3), and accumulation of curcumin in Curcuma zedoaria cell cultures. The results showed that, in cells treated with YE or SA, the expression levels of curcuminoid genes were 1.14- to 3.64-fold higher than the control (untreated cells), in which the YE exhibited a stronger effect in comparison with SA. Curcumin accumulation also tended to be similar to gene expression, curcumin contents in YE- or SA-treated cells were 1.61- to 2.53-fold higher than the control. The SA treatment at the fifth day of culture stimulated the curcumin accumulation and expression in all four genes compared to that at the beginning. While the YE treatments gave different results, the CzCURS1 and CzCURS3 genes were expressed strongly in cells that were treated at the beginning. However, the CzDCS and CzCURS2 genes showed the opposite expression pattern, they were activated strongly in the treatments at day five of the culture. However, the content of curcumin reached its maximum value on the fifth day of culture in all investigations.
Trichoderma asperellum Chi42 Genes Encode Chitinase
Loc, Nguyen Hoang,Quang, Hoang Tan,Hung, Nguyen Bao,Huy, Nguyen Duc,Phuong, Truong Thi Bich,Ha, Tran Thi Thu The Korean Society of Mycology 2011 Mycobiology Vol.39 No.3
Four Trichoderma strains (CH2, SH16, PQ34, and TN42) were isolated from soil samples collected from Quang Tri and Thua Thien Hue provinces in Vietnam. The strains exhibited high chitinolytic secretion. Strain PQ34 formed the largest zone of chitinase-mediated clearance (> 4 cm in diameter) in agar containing 1% (w/v) colloidal chitin. Analysis of the internal transcribed spacer regions of these strains indicated that they were Trichoderma asperellum. The molecular weights of the chitinases were approximately 42 kDa. Chitinase genes (chi42) of T. asperellum strains TN42, CH2, SH16, and PQ34 were 98~99% homologous to the ech42 gene of T. harzianum CB-Pin-01 (accession No. DQ166036). The deduced amino acid sequences of both T. asperellum strains SH16 and TN42 shared 100% similarity.
Vuong Tan Tu,Peter Estok,Gabor Csorba,Nguyen Truong Son,Hoang Trung Thanh,Le Quang Tuan,Tamas Gorfol 국립중앙과학관 2016 Journal of Asia-Pacific Biodiversity Vol.9 No.3
Three short field surveys were conducted in the Phia Oac-Phia Den Nature Reserve to assess the species diversity and endemism of local bat fauna. In combination with data from a previous study, 24 bat species of 5 families, representing > 20% of the national bat diversity, were recorded in the study area. The occurrence of several bat species restricted to montane forests, that is, Murina chrysochaetes, Pipistrellus coromandra, and a potentially new species Rhinolophus cf. macrotis, suggests that Phia Oac is an important area for bat conservation in Vietnam and thus more comprehensive studies need to be performed.
Thi Le Anh,Tan Man Minh,Tung Do Hoang,Tam Do Quang,Hoa Nguyen Minh 한국물리학회 2022 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.80 No.9
A first-principles calculation has investigated the structural, electronic, optical of the CdSe nanocrystal (NC) based on the generalized gradient approximation (GGA) method within the density functional theory. The geometric optimization of the unit cell is in good agreement with our experimental data. The band structure, density of states of the NC were analyzed. The results found that CdSe NC is a direct bandgap with an estimated 1.48 eV. We also report the results of dielectric functions and the refractive index (n) characterized. It is indicated that CdSe materials have excellent photoelectric properties potential applications in optoelectronic devices.
Nguyen Huu Khanh Nhan,Tran Hoang Quang Minh,Tan N. Nguyen,Miroslav Voznak 한국광학회 2017 Current Optics and Photonics Vol.1 No.6
Due to optimal advances such as chromatic performance, durability, low power consumption, highefficiency, long-lifetime, and excellent environmental friendliness, white LEDs (WLEDs) are widely usedin vehicle front lighting, backlighting, decorative lighting, street lighting, and even general lighting. In thispaper, the remote packaging WLEDs (RP-WLEDs) with bi-layer red-emitting Sr2Si5N8: Eu2+ and yellowemittingYAG:Ce phosphor was proposed and investigated. The simulation results based on the MATLABsoftware and the commercial software Light Tools indicated that the color rendering index (CRI) of bi-layerphosphor RP-WLEDs had a significant increase. The CRI had a considerable increase from 72 to 94. Inconclusion, the results showed that bi-layer red-emitting Sr2Si5N8:Eu2+ and yellow-emitting YAG:Cephosphor could be a prospective approach for manufacturing RP-WLEDs with enhanced optical properties.
Screening and Production of Manganese Peroxidase from Fusarium sp. on Residue Materials
( Nguyen Duc Huy ),( Nguyen Thi Thanh Tien ),( Le Thi Huyen ),( Hoang Tan Quang ),( Truong Quy Tung ),( Nguyen Ngoc Luong ),( Seung-moon Park ) 한국균학회 2017 Mycobiology Vol.45 No.1
In this study, we report the manganese peroxidase production ability from a Fusarium sp. strain using an inexpensive medium of agriculture residues of either rice straw or wood chips as carbon source. The highest manganese peroxidase activity on rice straw medium and on wood chips was 1.76 U/mL by day 9 and 1.91 U/mL by day 12, respectively.
Lan, Truong Thi Phuong,Huy, Nguyen Duc,Luong, Nguyen Ngoc,Quang, Hoang Tan,Tan, Trinh Huu,Thu, Le Thi Anh,Huy, Nguyen Xuan,Loc, Nguyen Hoang The Korean Society of Plant Biotechnology 2019 식물생명공학회지 Vol.46 No.3
The aim of this study is to evaluate the effect of yeast extract (YE) and salicylic acid (SA) on the expression of curcuminoid-biosynthesis genes (CzDCS and CURS1-3), and accumulation of curcumin in Curcuma zedoaria cell cultures. The results showed that, in cells treated with YE or SA, the expression levels of curcuminoid genes were 1.14- to 3.64-fold higher than the control (untreated cells), in which the YE exhibited a stronger effect in comparison with SA. Curcumin accumulation also tended to be similar to gene expression, curcumin contents in YE- or SA-treated cells were 1.61- to 2.53-fold higher than the control. The SA treatment at the fifth day of culture stimulated the curcumin accumulation and expression in all four genes compared to that at the beginning. While the YE treatments gave different results, the CzCURS1 and CzCURS3 genes were expressed strongly in cells that were treated at the beginning. However, the CzDCS and CzCURS2 genes showed the opposite expression pattern, they were activated strongly in the treatments at day five of the culture. However, the content of curcumin reached its maximum value on the fifth day of culture in all investigations.
Minh-Huy Dinh Dang,Trang Thi Thu Nguyen,Bao Quang Gia Le,Linh Ho Thuy Nguyen,Ngoc Xuan Dat Mai,My Van Nguyen,Phuong Hoang Tran,Tan Le Hoang Doan 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.111 No.-
Pd-incorporated Zr-based metal–organic framework containing sulfone groups, named Pd(II)BUT-11, wassuccessfully prepared by dispersing the sulfone-functionalized MOF in Pd(OAc)2 solution. X-ray diffraction,scanning electron microscopy, N2 isotherm sorption, X-ray photoelectron spectroscopy, and thermogravimetricanalysis were applied to determine the features of the Pd-incorporated MOF. In the catalyticstudies, Pd(II)@BUT-11 was shown to be an efficient heterogeneous catalyst for synthesizing various stilbenederivatives via the cross-coupling Heck reaction with DABCO-derived deep eutectic solvent (DES) asa green solvent. Notably, the catalyst and the solvent could be recovered and reused, with their activityand structure remaining stable throughout several trials. The method provides an alternative solution tothe conventional catalysts and solvents, resulting in a green and efficient stilbene synthesis process. Furthermore, the stilbenes were separated by extraction with ethyl acetate due to the low solubility ofDES with this solvent.
Genetic diversity of spotted scat (Scatophagus argus) in Vietnam based on COI genes
Huy Van Nguyen,Minh Tu Nguyen,Nghia Duc Vo,Nguyen Thi Thao Phan,Quang Tan Hoang 한국수산과학회 2022 Fisheries and Aquatic Sciences Vol.25 No.12
A spotted scat, Scatophagus argus, has a high nutritional value and is among Asia’s most widely consumed fish species. Thua Thien Hue’s consumption market considers this species to be of high economic value and requires protection and conservation of the population. However, the studies on the identification and genetic diversity of S. argus distributed in Vietnam are still lacking. Therefore, mitochondrial cytochrome c oxidase subunit I (COI) gene was utilized to distinguish different populations and investigate the genetic diversity of two populations of S. argus from Tam Giang lagoon, Thua Thien Hue province (n = 31) and Ca Mau province (n = 14). The sequencing results indicated 13 distinct haplotypes among 45 sequences. Five single nucleotide polymorphisms were observed to distinguish Hue spotted scat population. The S. argus population in Ca Mau province was higher haplotype diversity (Hd) and nucleotide diversity (π) than those of Thua Thien Hue province, which demonstrates that there are minor differences between haplotypes. There were genetic distances ranging from 0%–4% within the populations and 6.67% between the two populations. In addition to the sequencing, the comparison of morphology, biology, culture, and the growth rate was sufficient to distinguish the spotted scat S. argus in Thua Thien Hue from Ca Mau.