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제주지역 양식 넙치(Paralichthys olivaceus)의 점액포자충 감염조직에 대한 병리조직학적 관찰
이남실 ( Nam-sil Lee ),김아란 ( Aran Kim ),서한길 ( Han-gil Seo ),최혜승 ( He Sung Choi ),조미영 ( Miyoung Cho ) 한국수산과학회 2021 한국수산과학회지 Vol.54 No.5
In recent years, myxosporean infection from the cultured olive flounders Paralichthys olivaceus, have been frequently observed in Jeju island, South Korea. This study aimed to compare histopathological and molecular-biological methods of examining myxosporean infection from these flounders. Samples were obtained from affected individuals exhibiting emaciation or abdominal distention and a polymerase chain reaction (PCR) indicative of Parvicapsular anisocaudata, Enteromyxum leei and Kudoa septempunctata were initiated. Histopathological examination were conducted with H&E stained tissue sections, and then in-situ hybridization (ISH) reaction were processed with selected sections using P. anisocaudata, E. leei, K. septempunctata and Scuticociliate probes. Renal and intestinal tissue degeneration were common symptoms associated with all samples. Sever glomerular and renal tubular degeneration were evident, as were intestinal epithelial desquamation and spore formation in the epithelial cells. The results of conventional PCR analysis and ISH reactions revealed differences, and we suspect that various microparasites may have been associated with the symptoms manifested.
강원지역의 꽃매미 발생상황과 동계 기온변화에 따른 발생양상
정태성 ( Tae Sung Jeong ),황미란 ( Mi Ran Hwang ),문윤기 ( Youn Gi Moon ),이재홍 ( Jae Hong Lee ),이남길 ( Nam Gil Lee ),권순배 ( Soon Bae Kwon ),김인종 ( In Jong Kim ),최병렬 ( Byeong Ryel Choi ) 한국토양동물학회 2015 한국토양동물학회지 Vol.19 No.1
Lycorma delicatula in Gangwon province occurred firstly in 2008 in Chuncheon and Wonju. Its occurrence increased to 36.8 ha in 2011, but decreased to 11.8 ha, 8.7 ha in 2012 and 2013, respectively. In 2014 and 2015, it is again on the increase as 22.1 ha and 47.2 ha. For clarifying changes of occurrences, this study was compared with minium temperature of winter season, hatching rate of over-wintering eggs and occurrence status in grape orchard from 2011 to 2015. Elapsed days in - 10℃ and - 19℃ below of winter season greatly influenced on hatchability of over-wintering eggs and showed close correlation for change of occurrence area in Gangwon province.