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응고제가 우유두부의 물성 및 관능적 특성에 미치는 영향
우나리야,이민선,박수진,강명화 동아시아식생활학회 2004 동아시아식생활학회지 Vol.14 No.5
Different types of coagulant and characteristics of its concentration added in the process of making milk curd were compared in this study. The pH of whey decreased when the amount of coagulant increased. The turbidity of whey was decreased when 5% of acetic acid and lemon juice were put into as coagulants. The texture of milk curd made with cow's milk and skim milk was measured the highest when acetic acid and lemon juice were added at 3, 5% of their concentration. The fracturability of milk curd made only with cow's milk decreased as the concentration of each coagulant increased. The cohesiveness was decreased as concentration of coagulant increased. The springiness was slightly changed depending on its coagulant but didn't show much of difference. The gummminess of milk curd made with cow's milk was increased when 3, 5% of coagulant was added. The result of sensory evaluation of milk curd showed that preference of milk curd wasn't depending on types of milk nor its coagulant. Also, flavor preference showed better when lemon juice was added. Preference in texture of milk curd was the highest made with cow's milk and skim milk. Preference in taste was high when 10% of lemon juice was added to skim milk and preference in its appearance showed higher when the alum was added as a coagulant to both cow's milk and skim milk than other coagulants.
Pseudomonas fluorescens의 Salicylate hydroxylase를 암호하는 유전자의 클로닝
정유선,민경희,이나리 숙명여자대학교 자연과학연구소 1997 자연과학논문집 Vol.- No.8
Pseudomonas fluorescens SME11로 부터 salicylate hydroxylase를 암호하는 nahG 유전자를 클로닝하기 위하여 NAH플라스미드를 주형으로 하여 PCR을 수행하였다. PCR방법으로 얻은 1.6kb DNA절편을 pT7Blue(R)벡터에 삽입시켜 재조합 플라스미드 pNY1을 제조하였으며 이것을 E. coli에 형질전환하였다. 재조합 DNA를 제한효소로 처리하여 EcoRⅤ, KpnⅠ, PvuⅡ, StuⅠ등이 존재함을 확인하였으며, 이것으로 간단한 제한효소지도를 작성하였고 재조합 DNA를 한 방향으로 deletion하여, nahG유전자를 포함한 DNA절편의 염기서열을 결정하였다. 재조합 플라스미드인 pNY1에는 1,305bp크기의 open reading frame이 존재하였으며, salicylate hydroxylase에 해당하는 434개의 아미노산을 암호하였다. 아미노산서열을 비교한 결과, P.putida KF715와 84.1%, P.putida PpG7과 71.4%, P.putida S-1 과 48.7%의 homology를 보였으며, 또한 Sphingmonas sp.와 26.8%의 homology를 보였다. Pseudomonas fluorescens SME11 carrying NAH plasmid was isolated from wastewater. To clone nahG gene encoding salicylate hydroxylase, 1.6kb DNA fragment of PCR product from NAH plasmid was inserted to pT7Blue(R) vertor and resulting recombinant DNA was named pNY1. The transformants, E.coli SMY1, clone containing the recombinant plasmid pNY1 were able to convert salicylate to catechol and produced dark brown color caused by accumulation and auto-oxidation of catechol. Restriction endonuclease mapping of 1.6kb size of the insert of the recombinant plasmid pNY1 was carried out with EcoRⅤ, KpnⅠ, PvuⅡ, StuⅠ. By means of unidirectional ExoⅢ deletion and dideoxynucleotide chain termination, we determined the nucleotide sequence of the DNA fragment containing nahG gene. One open reading frame of 1,305 bp corresponding to 434 amino acids was found in the insert DNA. Deduced amino acid sequences of the nahG gene showed 84.1%, 71.4%, 48.7%, and 26.8% homologies to those from P.putida KF715, P. putida PpG7, P. putida S-1, and Sphingmonas sp., respectively.
Na Ri Lee,Hee Keun Shin,Sung Ho Choi,Ju Bum Kim,Jae Won Lim,Doh Young Park,Hyung Soo Mok 한국철도학회 2015 International Journal of Railway Vol.8 No.1
Due to the characteristics of the vehicle structure, the magnetic levitation train has a confined bottom space thus a study on miniaturization and weight reduction of auxiliary power unit is essential. This auxiliary power unit is an essential device used for illumination, air conditioning, heating and air brake equipment excluding the motor. The previous auxiliary power unit for magnetic levitation train has used the hard switching having a high switching frequency with heavy loss in order to reduce the size of filter reactor and transformer but the reduction in volume was not significant. In this paper, by reducing the loss, reducing the size of the cooling unit and by increasing the switching frequency using the soft switching of resonant converter, it has miniaturized and reduced the weight of filter reactor and transformer which occupy significant space in the auxiliary power unit. This study has verified the performance of 50KVA grade prototype through simulated interpretation and analysis, and compared the size and weight of auxiliary power unit of the previous magnetic levitation train.
<i>Undibacterium piscinae</i> sp. nov., isolated from Korean shiner intestine
Lee, So-Yeon,Kang, Woorim,Kim, Pil Soo,Kim, Hyun Sik,Sung, Hojun,Shin, Na-Ri,Whon, Tae Woong,Yun, Ji-Hyun,Lee, Jae-Yun,Lee, June-Young,Jung, Mi-Ja,Jeong, Yun-Seok,Tak, Euon Jung,Han, Jeong Eun,Hyun, D Society for General Microbiology 2019 International journal of systematic and evolutiona Vol.69 No.10
<P> A novel Gram-stain-negative, non-spore-forming, obligate aerobic, motile, rod-shaped, and flagellated bacterium, designated S11R28<SUP>T</SUP>, was isolated from the intestinal tract of a Korean shiner, <I>Coreoleuciscus splendidus</I>. Based on 16S rRNA gene sequences, strain S11R28<SUP>T</SUP> was identified as member of the genus <I>Undibacterium</I> in class <I>Betaproteobacteria,</I> and was closely related to <I>Undibacterium parvum</I> DSM 23061<SUP>T</SUP> (98.49%). The isolate grew at 4-25℃, pH 6-9, with 0% (w/v) NaCl, and grew optimally at 20℃, pH 8, in the absence of NaCl. The main cellular fatty acids were C<SUB>16 : 0</SUB> and summed features 3 (C<SUB>16 : 1</SUB>ω7c and/or C<SUB>16 : 1</SUB>ω6c). The strain possessed diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine as predominant polar lipids, and ubiquinone Q-8 as a respiratory quinone. The polyamine profile composed of 2-hydroxyputrescine, spermidine, putrescine, and benzoic acid. A genomic DNA G+Ccontent was 51.4mol%. The average nucleotide identity between strains S11R28<SUP>T</SUP> and U. parvum DSM 23061<SUP>T</SUP> was 78.66%. Thus, <I>Undibacterium piscinae</I> can be considered a novel species within the genus <I>Undibacterium</I> with the type strain S11R28<SUP>T</SUP> (=KCTC 62668<SUP>T</SUP> =JCM 33224<SUP>T</SUP>). </P>
Lee, Na-Ri,On, Hwa-Young,Min, Kyung-Hee 숙명여자대학교 환경과학연구센터 1996 환경과학논문집 Vol.4 No.-
A strain of Alcaligenes xylosoxydans SMN3 capable of utilizing biphenyl was isolated from the sewage. This strain grew not only on biphenyl, phenol, and benzoate, but also on salicylate. The ca-tabolism of biphenyl and salicylate is interrelated, since benzoate is common metabolic intermediates of biphenyl and salicylate. Enzyme levels in the extracts of benzoategrown stain SMN3 cells clearly showed that benzoate induced catechol 2,3oxygenase which in metacleavage enzyme of catechol, not induced catechol 1,2oxygenase. All the oxidative enzymes of biphenly and 2,3dihydroxybiphenyl (23DHBP) degradative pathways were induced when the cells were grown on biphenly or saliclyate, respectively. Biphenyl can be common inducer in the oxidation of biphenyl and 2,3dihydroxybiphenyl, in addition salicylate was also good inducer for the 2,3dihydroxybiphenyl dioxygenase. The two metabolic pathways for the degradation of biphenyl and salicylate are induced after growth on either biphenyl of salicylate, suggesting a common regulatory element. However, benzoate cannot induce the enzymes responsible for the oxidation of these compounds. It was clearly shown that biphenyl and salicylate were good inducers for indigo formation due to the activity of biphenyl dioxygenase. The strains of several bacteria that oxidize hydrocarbons including biphenyl to cisdihydrodiols also oxidize indole to indigo. Our results suggest that indigo formation is due to the combined activities of tryptophanase and biphenyl dioxygenase.
Lee, Na Ri,Shin, Hee Keun,Choi, Sung Ho,Kim, Ju Bum,Lim, Jae Won,Park, Doh Young,Mok, Hyung Soo The Korean Society for Railway 2015 International Journal of Railway Vol.8 No.1
Due to the characteristics of the vehicle structure, the magnetic levitation train has a confined bottom space thus a study on miniaturization and weight reduction of auxiliary power unit is essential. This auxiliary power unit is an essential device used for illumination, air conditioning, heating and air brake equipment excluding the motor. The previous auxiliary power unit for magnetic levitation train has used the hard switching having a high switching frequency with heavy loss in order to reduce the size of filter reactor and transformer but the reduction in volume was not significant. In this paper, by reducing the loss, reducing the size of the cooling unit and by increasing the switching frequency using the soft switching of resonant converter, it has miniaturized and reduced the weight of filter reactor and transformer which occupy significant space in the auxiliary power unit. This study has verified the performance of 50KVA grade prototype through simulated interpretation and analysis, and compared the size and weight of auxiliary power unit of the previous magnetic levitation train.
Lee, Na-Ri,Kim, Sun-Mi,Kim, Dae-Young Korean Chemical Society 2009 Bulletin of the Korean Chemical Society Vol.30 No.4
The catalytic enantioselective electrophilic fluorinations of active methine compounds promoted chiral palladium complexes have been developed. Treatment of $\beta$-keto phosphonates and $\beta$-ketoesters with N-fluorobenzenesulfonimide as the fluorine source under mild reaction conditions afforded the corresponding $\alpha$-fluorinated adducts in high yields with excellent enantiomeric excesses (up to 99% ee). These reactions can be conducted in alcoholic solvents without any precaution to exclude water and moisture.
Paenibacillus oceanisediminis sp. nov. isolated from marine sediment.
Lee, Jina,Shin, Na-Ri,Jung, Mi-Ja,Roh, Seong Woon,Kim, Min-Soo,Lee, Jung-Sook,Lee, Keun Chul,Kim, Young-Ok,Bae, Jin-Woo Society for General Microbiology 2013 International journal of systematic and evolutiona Vol.63 No.2
<P>A Gram-stain-negative, non-motile, aerobic, endospore forming and rod-shaped bacterium, designated strain L10(T), was isolated from marine sediment collected from the South Korean coast. The organism grew optimally under conditions of 30 C, 1?% (w/v) NaCl and pH 6.0. It was oxidase-negative and catalase-positive. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain L10(T) was associated with the genus Paenibacillus and most closely related to Paenibacillus barcinonensis BP-23(T) (98.2?% similarity). The major fatty acids of strain L10(T) were iso-C(14?:?0), anteiso-C(15?:?0) and iso-C(16?:?0). The cell-wall peptidoglycan was the A1γ type, and the predominant isoprenoid quinone was menaquinone-7. Strain L10(T) contained two unidentified lipids, an unidentified amino-phospholipid, phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol. The G+C content of the genomic DNA was 44 mol% and the DNA-DNA hybridization values with closely related strains were below 142?%. Based on phenotypic, genotypic, and phylogenetic data, strain L10(T) should be classified as a novel species within the genus Paenibacillus. The name Paenibacillus oceanisediminis sp. nov. is proposed. The type strain is L10(T) (?=?KACC 16203(T)?=?JCM 17814(T)).</P>