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Nepali, Sarmila,Ki, Hyeon-Hui,Lee, Ji-Hyun,Cha, Ji-Yun,Lee, Young-Mi,Kim, Dae-Ki UNKNOWN 2017 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.40 No.4
<P>Triticum aestivum sprout-derived polysaccharide (TASP) has anti-diabetic properties, but no information is available in regards to its protective effect against ethanol-induced hepatic injury. This study aimed to investigate the mechanism behind the protective role of TASP against ethanol-induced liver injury in vivo. Male C57BL/6 mice were administered ethanol with or without TASP for 10 consecutive days by oral gavage. Silymarin was administered in the same manner as a positive control. TASP reduced ethanol-induced hepatic lipid accumulation and serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels. TASP also prevented glutathione (GSH) depletion and increased the superoxide dismutase (SOD) in liver tissue. In addition, TASP significantly inhibited ethanol-induced cytochrome P450 2E1 (CYP2E1) activation, and upregulated the expressions of nuclear factor erythroid 2-related factor 2 (Nrf2) and hemeoxygenase-1 (HO-1), and downregulated NADPH oxidase genes in ethanol fed mice. Furthermore, the upregulation of Nrf2 was found to be regulated by a phosphatidylinositol 3-kinase (PI3K)/Akt pathway. TASP also attenuated hepatic injury by modulation of caspase-3 and apoptosis-associated mitochondrial proteins including B-cell lymphoma-2 (Bcl-2) and Bcl-2-associated X (Bax) in liver tissues of mice. The study demonstrated that TASP treatment protects against ethanol-induced hepatic injury via multiple pathways by inhibiting steatosis and improving antioxidant marker levels during hepatic injury. Such properties provide a basis for therapeutic agents against alcohol-induced liver injury.</P>
Nepali, Sarmila,Cha, Ji-Yun,Ki, Hyeon-Hui,Lee, Hoon-Yeon,Kim, Young-Ho,Kim, Dae-Ki,Song, Bong-Joon,Lee, Young-Mi World Scientific Publishing Company 2018 The American journal of Chinese medicine Vol.46 No.1
<P><I>Chrysanthemum indicum</I> (CI) is widely distributed in China and many parts of the tropical world, and has been reported to have antibacterial, antiviral, anti-oxidant and immunomodulatory effects, but no information is available on its effects on high fat diet (HFD)-induced obesity. This was undertaken to investigate the mechanism responsible for the effect of ethyl acetate fraction of CI (CIEA) on adipogenesis, <I>in vitro</I> and <I>in vivo</I> models of obesity. In the <I>in vitro</I> study, differentiating 3T3-L1 cells were treated with media to initiate differentiation (MDI) in the presence or absence of CIEA with different concentrations, and in the <I>in vivo</I> study, C57BL/6 mice were fed with HFD and administered CIEA daily for six weeks. <I>Garcinia cambogia</I> (GC) was used as the positive control, and was administered in the same manner as CIEA. Results showed CIEA reduced HFD-induced body weight gain, epididymal white adipose tissue (eWAT), and liver weight. In addition, CIEA significantly decreased serum lipid profiles, including total cholesterol (TC), triglyceride (TG) and low density lipoprotein cholesterol (LDLc) and increased high density lipoprotein cholesterol (HDLc) levels. Furthermore, CIEA also reduced leptin levels and increased adiponectin levels in serum, and significantly decreased peroxisome proliferator-activated receptor <TEX>$ \gamma $</TEX> (PPAR<TEX>$ \gamma $</TEX>) and CCAAT/enhancer-binding protein (C/EPBs) levels, but increased PPAR<TEX>$ \alpha $</TEX> level and the phosphorylation of AMP-activated protein kinase (AMPK) in eWATs and in the liver tissues of HFD fed obese mice. Taken together, these results indicate CIEA might be beneficial for preventing obesity.</P>
Babu Ram Nepali,John Skartveit,Chitra Bahadur Baniya 국립중앙과학관 2021 Journal of Asia-Pacific Biodiversity Vol.14 No.3
This study aimed to find out the roles of altitude, slope aspect, and soil factors in species richness in theNarapani-Masina landscape, Arghakhanchi, Nepal. We surveyed forest plant communities by sampling192 statistically representative 10 10 m sample plots from 64 sites representing all 100 elevation bandsof 4 slope aspects (East, South, West, and North) of the landscape. We considered the species present inall plots of each 100 m contour elevation of four aspects as species richness of that elevation band. Wecollected the soil sample from 10 10 10 cm plot of 10 cm below the ground level at four corners ofplant sampled plots to estimate the soil nutrients in a laboratory. We estimated the correlation,regression, Tukey post hoc test, PerMANOVA, and canonical correspondence analysis to show the relationshipbetween environment and response variables. We found a significant negative relationshipbetween species richness and altitude and soil nitrogen. The species richness showed a unimodalstructure with elevation. The slope aspect showed a significant effect on species composition, but not onspecies richness. This study concludes that the increasing trend of soil N, P, and K with altitude showed anegative relation with species richness.
POUDEL, BARUN,NEPALI, SARMILA,XIN, MINGJIE,KI, HYEON-HUI,KIM, YOUNG-HO,KIM, DAE-KI,LEE, YOUNG-MI SPANDIDOS PUBLICATIONS 2015 MOLECULAR MEDICINE REPORTS Vol.12 No.2
<P>The present study aimed to compare the potential anti-adipogenic effects and underlying mechanisms of the luteolin, isoscoparin and isoorientin flavonoids, purified from Triticum aestivum sprout (TA) in 3T3-L1 cells. The cells were treated with different concentrations of flavonoids for 8 days and the lipid accumulation was assessed using Oil-Red-O staining. The expression levels of the transcription factors and the genes involved in adipogenesis in the cells were assessed by reverse transcription-quantitative polymerase chain reaction and western blotting. The results demonstrated that 10 μM luteolin, isoscoparin or isoorientin inhibited lipid deposition in the cells by 74, 63 and 65%, respectively. The flavonoids also significantly inhibited the transcriptional regulators of adipogenesis, including peroxisome proliferator-activated receptor-γ, CAAT/enhancer binding protein-α and sterol regulatory element binding protein (SREBP)-1c, compared with the control cells. Similarly, there was a significant downregulation of the adipocyte specific markers associated with lipid metabolism, including activating protein-2, fatty acid synthase, hormone-sensitive lipase and lipoprotein lipase, in the flavonoid treated cells. Notably, the cells treated with the flavonoids demonstrated increased expression levels of the insulin-induced genes, insig-1 and insig-2, which may have inhibited the activation of the adipogenic transcription factor, SREBP, eventually leading to the inhibition of adipogenesis. Taken together, these results revealed that the flavonoids from TA possessed an inhibitory effect on adipogenesis through downregulation of adipogenic transcription factors and genes associated with lipid metabolism, and the upregulation of insig 1 and 2, suggesting that the flavonoids from TA may be potential therapeutic agents for the prevention and treatment of obesity.</P>
김성희,Rajendra Nepali,유명훈,이광선,정종우 대한청각학회 2017 Journal of Audiology & Otology Vol.21 No.2
Background and Objectives: The mutation of the gap junction protein beta 2 (GJB2) geneis the predominant cause of autosomal recessive non-syndromic hearing loss. The purposeof this study was to evaluate the speech perception outcome after cochlear implantation accordingto the presence of a GJB2 mutation. Subjects and Methods: During the period fromMarch 2004 to February 2005, 38 patients underwent cochlear implantation at Asan MedicalCenter. Genetic factors and speech perception were evaluated in all subjects, and the patientswere grouped according to the presence of a GJB2 mutation. The two groups werecarefully matched according to the age at cochlear implantation. We analyzed four mutationsin the GJB2 gene: 35delG, 167delT, 235delC, and E114G. Speech perception outcomeswere measured using the open set, 1 and 2 syllables, the comprehension test, the MeaningfulAuditory Integration Scale, the categories of auditory performance, and the Speech IntelligibilityRating scores. The evaluations were performed before the operation, 6 and 12 monthsthereafter, and then annually up to nine years after cochlear implantation. Results: Fifteenpatients had bi-allelic GJB2 mutations (11 with E114G and 4 with 235delC), whereas the remaining23 had wild type alleles. For the age-matched analysis, 14 patients were selectedand divided into two groups of 7 subjects each: GJB2 mutation and no mutation (i.e., deafnessof unknown origin). Overall, all patients showed improvement of speech perceptionoutcome after cochlear implantation. There was no difference in the improvement betweenpatients with and without GJB2 mutations at the 5-year and 9-year follow up. The pattern ofimprovement throughout the duration of the follow-up also showed no difference betweenthe two groups. Conclusions: Similar outcomes of speech perception are expected aftercochlear implantation in pediatric patients with or without GJB2 mutation.