간호대학생의 단기 해외어학연수를 통한 자기인식 경험

손미라 ( Son¸ Mira ),김신향 ( Kim¸ Sinhyang ) 한국간호과학회 정신간호학회(구 대한간호학회정신간호학회) 2021 정신간호학회지 Vol.30 No.1

Purpose: The purpose of this study was to explore the experience of self-awareness of nursing students through a short-term overseas language study. Methods: Study subjects were 10 nursing students, and collected data were analyzed with open coding, axial coding, selective coding, and process analysis based on Strauss and Corbin's grounded theory (1998). Results: In total, 23 sub-categories and 12 categories were generated. The 12 categories included "Longing for a bigger world", "Don't want to be intimidated", "Interest in English", "Tension about local classes", "Feeling of pushing the limit", "Getting along with natives", “Making Korean American University students a role model", "Self-observe confronting unfamiliarity", "Self-encourage about enduring hardships", "Self-expansion for a better self", "Recognize self-transformation", "Focusing on the reality". Nursing students' self-awareness experience through a short-term overseas language study was a process of solving the phenomenon of 'feeling of pushing the limit', and a derived core category was 'surpass the feeling of pushing the limit and take a step forward'. Conclusion: Study participants experienced self-awareness. These results will not only expand our understanding about self-awareness of nursing students and educators but will also provide information for developing a short-term overseas study program.

The KAAACI/KDA Evidence-Based Practice Guidelines for Chronic Spontaneous Urticaria in Korean Adults and Children: Part 1. Definition, Methodology and First-line Management

송우정,Mira Choi,Dong Hun Lee,Jae-Woo Kwon,Gun-Woo Kim,Myung Hwa Kim,Mi-Ae Kim,Min-Hye Kim,Byung-Keun Kim,Sujeong Kim,Joung Soo Kim,Jung Eun Kim,Ju Young Kim,Joo Hee Kim,Hyun Jung Kim,Hye One Kim,Hyo-Bin 대한천식알레르기학회 2020 Allergy, Asthma & Immunology Research Vol.12 No.4

Chronic spontaneous urticaria (CSU) is defined as the occurrence of spontaneous wheals, angioedema, or both for >6 weeks in the absence of specific causes. It is a common condition associated with substantial disease burden both for affected individuals and societies in many countries, including Korea. CSU frequently persists for several years and requires high-intensity treatment; therefore, patients experience deteriorations in quality of life and medication-associated complications. During the last decade, there have been major advances in the pharmacological treatment of CSU and there is an outstanding need for evidence-based guidelines that reflect clinical practice in Korea. The guidelines reported here represent a joint initiative of the Korean Academy of Asthma, Allergy and Clinical Immunology and the Korean Dermatological Association, and aim to provide evidence-based guidance for the management of CSU in Korean adults and children. In Part 1, disease definition, guideline scope and development methodology as well as evidence-based recommendations on the use of antihistamines and corticosteroids are summarized.

Flow and surface pressure field measurements on a circular cylinder with impingement of turbulent round jet

Kim, Mirae,Li, Yongzeng,Peng, Di,Yeom, Eunseop,Kim, Kyung Chun Elsevier 2019 Experimental thermal and fluid science Vol.105 No.-

<P><B>Abstract</B></P> <P>When the circular jet impinges onto a cylinder, the three-dimensional (3D) curved wall jet was attached to the wall due to the Coanda effect and the flow separation was delayed with increasing Reynolds number and impinging angle. These interesting phenomena can be studied in depth through a variety of parameters and measurement methods. Time-resolved stereo PIV measurement was conducted to investigate the 3D flow characteristics with instantaneous velocity and vorticity fields. Pressure sensitive paint (PSP) method was used to examine the pressure distribution on the cylinder surface when a circular jet impinges on it. The effects of the impingement angle, Reynolds number, and diameter ratio between the jet nozzle and the cylinder (d/D) were examined at a fixed nozzle to wall distance ratio of two (H/d = 2). Experimental results show that the pressure gradient in the flow direction decreases and the separation is delayed when the Reynolds number and impingement angle are increased at the same distance ratio. As the diameter ratio decreases with the same impingement angle and Reynolds number, the maximum suction pressure increases, and the pressure gradient in the wall jet flow direction increases. The three-dimensional nature of the surface pressure distribution changes with the flow parameters, which are well explained with three-dimensional flow field measurements in the curved 3D wall jet due to the Coanda effect.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Surface pressure distribution was measured when a circular jet impinges on a cylinder. </LI> <LI> Effects of the impingement angle, Reynolds number, and diameter ratio were examined. </LI> <LI> Pressure gradient in flow direction decreases when the impingement angle increases. </LI> <LI> The three-dimensional nature of the surface pressure changes with the flow parameters. </LI> </UL> </P>

Derivation of putative brain endothelial cells from porcine induced pluripotent stem cells

Mirae Kim,Sang-Hwan Hyun 한국수정란이식학회 2017 한국수정란이식학회 학술대회 Vol.2017 No.05

The use of pigs in neuroscience has increased over the past years because the pigs are closely related to humans in terms of anatomy and physiology. Especially, the blood-brain barrier (BBB) maintains the homeostatic microenvironment in the central nervous system (CNS) and they can provide a valuable tool for studying the neurobiology. However, only a few putative blood-brain barrier (BBB) models have been generated by co-culture of porcine primary cells. The fundamental problem is that they lose some of their phenotypes when maintained in vitro for long-term culture. To establish improved in vitro porcine BBB models, we differentiated novel brain microvascular endothelial cells (BMECs) from porcine induced pluripotent stem cells (iPSCs) using a modified human-based protocol. Briefly, the dissociated single cells from iPSCs were seeded in Geltrex. For differentiation, cells were maintained for 3 days of expansion and then switched to unconditioned medium (UM) lacking bFGF for 6-7 days. Then, we subcultured cells onto collagen/fibronectin coated plates and changed BMEC medium for 2-3 weeks. About two weeks later, we observed a cluster of round cells surrounded by spindle shaped adherent cells termed as colony-forming units (CFU) of putative BMECs. Over time, the cluster of cells disappears and remained adherent spindle-shaped cells showed properties of endothelial cells. Although further studies will be needed, this study would be a great comparative analysis of the porcine and human in vitro BBB model.

Oral Supplementation with Cocoa Extract Reduces UVB-Induced Wrinkles in Hairless Mouse Skin

Kim, Jong-Eun,Song, Dasom,Kim, Junil,Choi, Jina,Kim, Jong Rhan,Yoon, Hyun-Sun,Bae, Jung-Soo,Han, Mira,Lee, Sein,Hong, Ji Sun,Song, Dayoung,Kim, Seong-Jin,Son, Myoung-Jin,Choi, Sang-Woon,Chung, Jin Ho Elsevier 2016 The Journal of investigative dermatology Vol.136 No.5

<P>Cacao beans contain various bioactive phytochemicals that could modify the pathogeneses of certain diseases. Here, we report that oral administration of cacao powder (CP) attenuates UVB-induced skin wrinkling by the regulation of genes involved in dermal matrix production and maintenance. Transcriptome analysis revealed that 788 genes are down-or upregulated in the CP supplemented group, compared with the UVB-irradiated mouse skin controls. Among the differentially expressed genes, cathepsin G and serpin B6c play important roles in UVB-induced skin wrinkle formation. Gene regulatory network analysis also identified several candidate regulators responsible for the protective effects of CP supplementation against UVB-induced skin damage. CP also elicited antiwrinkle effects via inhibition of UVB-induced matrix metalloproteinases-1 expression in both the human skin equivalent model and human dermal fibroblasts. Inhibition of UVB-induced activator protein-1 via CP supplementation is likely to affect the expression of matrix metalloproteinases-1. CP supplementation also downregulates the expression of cathepsin G in human dermal fibroblasts. 5-(3',4'-Dihydroxyphenyl)-gamma-valerolactone, a major in vivo metabolite of CP, showed effects similar to CP supplementation. These results suggest that cacao extract may offer a protective effect against photoaging by inhibiting the breakdown of dermal matrix, which leads to an overall reduction in wrinkle formation.</P>

Derivation of Canine induced Pluripotent Stem Cells using an Integration-Free Method

Mirae Kim,Seon-Ung Hwang,Sang-Hwan Hyun 한국동물생명공학회(구 한국동물번식학회) 2017 발생공학 국제심포지엄 및 학술대회 Vol.2017 No.10

Canine induced Pluripotent Stem Cells (iPSCs) can provide great potential for regenerative veterinary medicine and may assist in the development of new therapeutics and pre-clinical studies for both dogs and human. To date, there have been several reports on the generation of canine iPSCs using retroviral or lentiviral transduction of Yamanaka’s factors. However, there is no report of canine iPSCs generated by genomic integration-free methods. According to previous studies, a polycistronic and synthetic self-replicating RNA system was developed for generating human iPSCs by the RNA replicon of Venezuelan Equine Encephalitis (VEE) virus. The VEE replicon is a positive-sense and single-stranded RNA which is similar with cellular mRNA containing a 5’ cap and poly (A) tail. It is no potential for genomic DNA integration problems because it does not use a DNA intermediate. Here, we investigated to generate canine iPSCs by a single transfection of the VEE-reprogramming factor (VEE-RF) RNA. To generate integration- free canine iPSCs, the VEE-OKS-iG RNA that expresses four reprogramming ORFs (hOct4, hKlf4, hSox2 and hGlis1) was transfected. Also, B18R mRNA was co-transfected to reduce immune response by VEE replicon. Putative canine iPSC colonies first appeared between day 15-25. Interestingly, they have two distinct types of initial canine iPSC colonies. They were identified by immunohistochemistry of live cells using TRA-1-60 antibody and also showed clear alkaline phosphatase (AP) activity. Further study will be required to analyze the characterization for a clinical application, and the non-integrating and self-replicating VEE RNA replicon system has the potential to make a great contribution to generating clinically applicable canine iPSCs.

Effect of frost on phosphorescence for thermographic phosphor thermometry

Kim, Dong,Kim, Mirae,Kim, Kyung Chun IOP Pub 2017 Measurement science & technology Vol.28 No.12

<P>In this study, we analyzed phosphorescence lifetime and its accuracy by growing frost for thermographic phosphor thermometry in a low-temperature environment. Mg<SUB>4</SUB>FGeO<SUB>6</SUB>:Mn particles were coated on an aluminum plate and excited with a UV-LED to obtain phosphorescence signals. The surface temperature was maintained at  −20, −15, −10 °C, and the phosphorescence signal was acquired as the frost grew for 3700 s. The lifetime was calculated and compared with the calibration curve under no-frost conditions. The error of the measured lifetime was within 0.7% of that in the no-frost conditions. A 2D surface temperature profile of the target plate was successfully obtained with the frost formation.</P>

Flow Characteristics of Impinging Jet onto a Cylinder

Mirae Kim(김미래),Hyun Dong Kim(김현동),Kyung Chun Kim(김경천) 한국가시화정보학회 2016 한국가시화정보학회 학술발표대회 논문집 Vol.2016 No.12

A New Method for Aortic Valve Planimetry with High-Resolution 3-Dimensional MRI and Its Comparison with Conventional Cine MRI and Echocardiography for Assessing the Severity of Aortic Valvular Stenosis

Kim Hae Jin,Choe Yeon Hyeon,Kim Sung Mok,Kim Eun Kyung,Lee Mirae,Park Sung-Ji,Ahn Joonghyun,Carriere Keumhee C. 대한영상의학회 2021 Korean Journal of Radiology Vol.22 No.8

Objective: We aimed to compare the aortic valve area (AVA) calculated using fast high-resolution three-dimensional (3D) magnetic resonance (MR) image acquisition with that of the conventional two-dimensional (2D) cine MR technique. Materials and Methods: We included 139 consecutive patients (mean age ± standard deviation [SD], 68.5 ± 9.4 years) with aortic valvular stenosis (AS) and 21 asymptomatic controls (52.3 ± 14.2 years). High-resolution T2-prepared 3D steady-state free precession (SSFP) images (2.0 mm slice thickness, 10 contiguous slices) for 3D planimetry (3DP) were acquired with a single breath hold during mid-systole. 2D SSFP cine MR images (6.0 mm slice thickness) for 2D planimetry (2DP) were also obtained at three aortic valve levels. The calculations for the effective AVA based on the MR images were compared with the transthoracic echocardiographic (TTE) measurements using the continuity equation. Results: The mean AVA ± SD derived by 3DP, 2DP, and TTE in the AS group were 0.81 ± 0.26 cm2, 0.82 ± 0.34 cm2, and 0.80 ± 0.26 cm2, respectively (p = 0.366). The intra-observer agreement was higher for 3DP than 2DP in one observer: intraclass correlation coefficient (ICC) of 0.95 (95% confidence interval [CI], 0.94–0.97) and 0.87 (95% CI, 0.82–0.91), respectively, for observer 1 and 0.97 (95% CI, 0.96–0.98) and 0.98 (95% CI, 0.97–0.99), respectively, for observer 2. Inter-observer agreement was similar between 3DP and 2DP, with the ICC of 0.92 (95% CI, 0.89–0.94) and 0.91 (95% CI, 0.88–0.93), respectively. 3DP-derived AVA showed a slightly higher agreement with AVA measured by TTE than the 2DP-derived AVA, with the ICC of 0.87 (95% CI, 0.82–0.91) vs. 0.85 (95% CI, 0.79–0.89). Conclusion: High-resolution 3D MR image acquisition, with single-breath-hold SSFP sequences, gave AVA measurement with low observer variability that correlated highly with those obtained by TTE.

Revision of NIER Test Guideline for Chemical Assessment

Mirae Kim(김미래),In-Young Kim(김인영),So-Young Park(박소영),Handule Lee(이한들),Jung-Taek Kwon(권정택),Hyunmi Kim(김현미),Pilj Kim(김필제),Kwangsik Park(박광식) 환경독성보건학회 2014 한국독성학회 심포지움 및 학술발표회 Vol.2014 No.11