http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
구기자, 황기, 결명자, 둥글레, 복분자의 활성산소 제거를 통한 당뇨 혈관
Young Min Lee,Kyoung Hee Seo,Kang Min Park,Dong Hyun Park,Jin Seong Kim,Beong Ou L,Jun Ho Lee 한국약용작물학회 2016 한국약용작물학술대회 발표집 Vol.2016 No.10
Background : ROS produced by oxidative stress damaged endothelial cells, and cause a variety of vascular complications. In diabetic hyperglycemia state, ROS increase. The polyol pathway occur in diabetic complications, the excess glucose is absorbed into the polyol pathway when aldose reductase increased, NADPH changes it to sorbitol. Glutathione (GSH) removes ROS. GSH level is reduced by glutathione reductase, using NADPH as an electron donor. Activation of the polyol pathway decrease NADPH, and GSH also reduced. As a result, ROS is increased. In diabetic hyperglycemia state, Glycolysis increases. Effects of increased glycolysis, protein kinase C (PKC) is increased. NAD(P)H oxidase, stimulated by PKC-dependent pathway, increases ROS in the cell. In this study, we measured the ROS scavenging activity of 5 natural products (Lycii fructus, Astragalus membranaceus, Cassia Tora, Polygonatum odoratum, Rubus Coreanus), to confirm the efficacy as diabetic antioxidants. Methods and Results : We extracted 5 natural product by distilled water and ethanol. DPPH radical scavenging activity was significantly higher in Lycii fructus, Rubus coreanus. ABTS radical scavenging activity was better Rubus coreanus, Lycii fructus, Cassia Tora. In addition to, Rubus coreanus, Cassia Tora, Lycii fructus was comparatively higher reducing power activity than other natural products. And total phenolic and flavonoid contents were much higher in Rubus coreanus compared with other extracts. Conclusion : These results suggest that Lycii fructus, Rubus coreanus can be applied as diabetic antioxidant that prevent vascular complications caused by ROS.
F3570 추출물의 ROS 발생 억제를 통한 RANKL로 유도된 파골세포 분화 조절
Kyoung Hee Seo,Young Min Lee,Md Abul Hasnat,Jin Seong Kim,Jun Ho Lee,Beong Ou Lim 한국약용작물학회 2016 한국약용작물학술대회 발표집 Vol.2016 No.05
Background : Osteoclasts as multinucleated cells originate from hematopoietic monocyte/ macrophage precursor cell, shows the bone absorption through the commitment, differentiation, fusion, and bone resorption stages by regulation of M-CSF and RANKL. It has been reported a significant negative correlation between the increase of oxidative stress and the bone density, and when RANKL reaction to the osteoclasts precursor cells is mainly generated ROS is due to increased activity of NADPH oxidase1 (NOX1), and these ROS act as a factor which promotes osteoclasts differentiation. Thus, RANKL signaling process is important that excessive osteoclast formation and differentiation inhibited through the regulation of each step. Methods and Results : F3570 ethanol extract showed relatively high activity at in-vitro antioxidant activity. F3570 water extract inhibited ROS generation in RAW 264.7 cells stimulated with H2O2 and RANKL, even at low concentrations. The inhibitory effect of osteoclast differentiation on F3570 water extract was confirmed that shown through NF-κB pathway, MAPK pathway including ERK and JNK. F3570 ethanol extract is considered to be regulated by the p38 MAPK and the other signaling pathway. Also, F3570 both water and ethanol extract were significantly reduced gene expression such as TRAP, calcitonin receptors and integrin β3 of RANKL-induced mature osteoclast in the bone resorption stage. Conclusion : Through this study, F3570 extract revealed an outstanding inhibitory effect and signaling mechanisms in osteoclast differentiation induced by RANKL. These results suggest that F3570 is bone diseases associated with aging or osteoporosis caused by menopause in an aging society is expected to be a superior candidate for the treatment or the prevention
LPS 처리한 RAW 264.7에서 MAPK Signaling의 하위조절을 통한 우슬 추출물의 항산화 및 항염 효과
Kyoung Hee Seo,Young Min Lee,Mithun Ghosh,Kang Min Park,Dong Hyun Park,Jin Seong Kim,Jun Ho Lee,Beong Ou Lim 한국약용작물학회 2016 한국약용작물학술대회 발표집 Vol.2016 No.10
Background : Achyranthes japonica Nakai (AJ) is a perennial herb with a wide distribution in East Asia including Korea, China, and Japan, and it is mainly used as a medicinal plant. In Korea, AJ has been widely used to control pain and improve symptoms in OA patients. AJ contains several important phytochemicals such as saponins, inokosterone, ecdysterone, and oleanolic acid bisdesmoside. Methods and Results : The aim of this work was to investigate the antioxidant and anti-inflammatory activities of fermented and ethanol extracts of Achyranthes japonica Nakai (AJ). The extracts showed strong reductive power and nitrite scavenging, hydroxyl radical scavenging, superoxide radical scavenging, and DNA damage prevention activities. Treatment of RAW 264.7 macrophages with AJ inhibited lipopolysaccharide (LPS)-induced NO secretion and iNOS expression without affecting cell viability. AJ also inhibited cyclooxygenase 2 (COX-2) expression, leading to the suppression of COX-2-derived prostaglandin E2 production. These inhibitory effects of AJ were accompanied by reduced production of tumor necrosis factor-α and interleukins (IL)-1β, -6, and -10. Furthermore, AJ suppressed LPS-induced phosphorylation of extracellular signal regulated kinase, c-Jun N-terminal kinase, and p38. Moreover, AJ inhibited malondialdehyde production and myeloperoxidase activity in LPS-stimulated RAW 264.7 cells. Conclusion : The antioxidant activity of plants is closely related to their medicinal properties and is widely used as a parameter to determine the bioavailability of medicinal plants. The antioxidant and biological activities of AJ extracts might be due to the synergistic actions of multiple bioactive compounds. It can be concluded that AJ extracts are a potential source of biologically important drug candidates.
민정기,김태상,조병영,김상명,민병준,Min. Jeong-Ki,Kim. Taesang,Cho. Byung-Young,Kim. Sang-Myoung,Min. Beong-Jun 한국방재학회 2014 한국방재학회 학술발표대회논문집 Vol.2014 No.1
본 연구에서는 국내외에서 제작되어 사용되고 있는 면진장치의 시험규격에 대한 국내외 동향을 살펴보았으며, 국제규격에서 요구하는 수준에 부합하는 면진장치 시험평가시스템을 개발하였다. 면진장치의 경우 건축, 토목구조물, 정보통신시설 및 원자력시설에 적용되어 지진재해 발생시 요구되는 성능을 정확히 만족하는지에 대한 확인이 필요하며, 현재까지는 면진장치 제작업체의 자체 품질검사를 위해 구축한 시험기에서 직접 이루어지는 입회시험으로 제품성능을 평가해 왔다. 그러나 면진장치의 국내 판매 뿐 아니라 국내 제품의 해외 판매 시 국제적으로 공인될 수 있는 시험기관에서 성능을 평가한 객관적이고 신뢰성 있는 인증서의 요구가 증가하고 있는 실정이다. 이에 따라 ISO 22762 등 국제규격에서 요구하고 있는 면진장치의 성능에 대해 살펴보고, 그에 따른 국내 면진장치 시험평가 시스템의 장단점을 파악하였으며, 이를 통해 기존 면진장치 시험평가에 최적화된 새로운 면진장치 평가시스템을 개발하였다. 이 연구에서는 면진장치 시험평가시스템의 개발에서부터 면진장치 시험평가시스템의 성능을 파악하기 위한 성능실험 등 장비 개발에 대한 전반적인 과정에 대해 설명한다. 또한 새로 개발된 면진장치 시험평가시스템 장비를 활용한다면, 국내 기업의 해외진출 시 유용하게 사용될 수 있을 것으로 기대된다.