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Islam, Md. Mazharul,Yesmin, Reshma,Jung, Min-Jung,Kim, Hong-Yul,Kim, Chang-Kil,Lim, Ki-Byung Korean Society for Horticultural Science 2020 Horticulture, Environment, and Biotechnology Vol.61 No.2
<P> This study aimed to determine the morphological and cytogenetic differences in an intraspecific Asiatic F<SUB>1</SUB> <I>Lilium</I> hybrid. The results indicated that leaf color and shape, flower morphology including color, spot size on petal, and filament color in the F<SUB>1</SUB> hybrid showed significant variations as compared to parents, while days to flowering were similar to those of the parents. Moreover, stem height, leaf number, and leaf width showed distinct variations. Interestingly, the intraspecific F1 progeny obtained from Asiatic lily parents showed higher pollen viability. In addition, FISH results revealed significant variation in the number of 18S rDNA and 5S rDNA loci identified in both parents and the F<SUB>1</SUB> progeny. The female (2n=4x=48) parent had 12 loci of 18S rDNA, whereas the male (2n=2x=24) parent and the F<SUB>1</SUB> (2n=3x=36) had 8 and 11 loci of 18S rDNA, respectively. Moreover, the F<SUB>1</SUB> progeny had 9 loci of 5S rDNA compared with the 7 and 4 loci identified in the female and male parents, respectively. All 5S rDNA signals were observed on the long arm in both the female parent and the F<SUB>1</SUB>; however, one 5S rDNA signal was observed on the short arm in the male parent. Therefore, this study demonstrated that the distribution of ribosomal DNA was greatly different in the F<SUB>1</SUB> progeny than that of parents. </P>
MD Mazharul Islam,Hye-Min Lee,Deen Mohammad Deepo,Hong Yul Kim,Ki-Byung Lim 한국원예학회 2021 한국원예학회 학술발표요지 Vol.2021 No.10
This study was aimed at differentiating parental genomes, examining intergenomic composition, and mapping mitotic metaphase chromosomes by localizing parental and 18S rDNA probes in seven interspecific hybrid progenies that originated from Lilium longiflorum. Since in situ hybridization has not been previously used in lily breeding, flow cytometry was used in conjunction with genomic and fluorescent in situ hybridization to determine the genomic contribution of each parent to the interspecific progenies. A significant variation was observed in the DNA content, chromosome length, and 18S loci in F₁ as compared to the female and male parents. L. longiflorum showed nearly two times higher DNA content than the male parents and L. longiflorum × Asiatic progenies, but eight times higher than L. longiflorum × L. hansonii. Genomic in situ hybridization results revealed that both female and male parents contributed an equal number of chromosomes to their interspecific F₁ offspring. Fluorescent in situ hybridization mapping revealed that 18S rDNA had 8, 6 and 7 loci in L. longiflorum parents, i.e., White heaven, Bright tower, and White tower, respectively, whereas each Asiatic cultivar and L. hansonii used as male showed 8 and 12 loci respectively. Interspecific progenies showed 8 and 7 loci in LA, and 10 to 11 in LM hybrids. These cytogenetic results implied equal genetic and chromosomal contribution from both parents to their intergenomic progenies. Therefore, this combined cytogenetic method has the potential to be an affordable and time-saving approach in lily breeding that could determine the status of hybrids and their genomic origin while achieving physical mapping and detecting genes in different genomes.
Morphological Characteristics and FISH Analysis of Hibiscus F1 Hybrids and Parental Lines
조영관,Islam MD Mazharul,김창길,Hong-Yul Kim,임기병 한국원예학회 2019 원예과학기술지 Vol.37 No.5
In this study, the characteristics of ‘Tohagolred’, ‘Daewangchun’ generated by a reciprocal cross between Hibiscus sinosyriacus ‘Seobong’ and H. syriacus ‘Samchully’, and the correspondingparental lines were investigated and DNA content was measured by flow cytometry. FISH detection of 5S and 45S rDNA was conducted to analyze the parental and progeny karyotypes. The leaf margin was serrate for ‘Samchully’ and crenate for ‘Seobong’. The leaf margin of ‘Daewangchun’ was crenate, the avarage leaf length was 8.2 cm, and the average leaf width was 5.4 cm. The leaf margin of ‘Tohagolred’ was crenate, the average leaf length was 7.2 cm, and the average leaf width was 5.7 cm. The diameter of the ‘Daewangchun’ flower was 13.8 cm, which was 2 cm larger than the parents, and the red eye radius was 2.1 cm, which was approximately 1 cm larger than that of the parents. ‘Daewangchun’ appeared pink in color, similar to ‘Samchully.’ The flower diameter of ‘Tohagolred’ was 12.6 cm, which was 1 cm larger than that of the parents; the red eye length radius was 1.1 cm and approximately 1 cm smaller than that of the parents. ‘Tohagolred’ also appeared pink in color, similar to ‘Samchully.’ The DNA content was 1928.77 Mbp/1C (1.97 pg/1C) for ‘Seobong’ and 2820.34 Mbp/1C (2.88 pg/1C) for ‘Samchully’. Moreover, the DNA content of ‘Daewangchun’ and ‘Tohagolred’ was 1975.88 Mbp/1C (2.88 pg/1C) and 1914.70 Mbp/1C (1.97pg/1C), respectively. DAPI staining showed that ‘Seobong’ was 2n = 80 and ‘Samchully’ was 2n =126 as parents. However, Hibiscus F1 cultivars ‘Daewangchun’ and ‘Tohagolred’ were 2n = 82.FISH analysis revealed that ‘Seobong’ had six 45S rDNA signals and two 5S rDNA signals. In contrast, ‘Samchully’ had six 45S rDNA signals and three 5S rDNA signals. Both ‘Daewangchun’and ‘Tohagolred’ had seven 45S rDNA signals and two 5S rDNA signals. These results provide a foundation for genetic analysis and cultivation of new crops of Hibiscus spp.