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Protein nanoarrays on a highly-oriented lamellar surface
Matsusaki, Michiya,Omichi, Masaaki,Kadowaki, Koji,Kim, Bong Hoon,Kim, Sang Ouk,Maruyama, Ikuro,Akashi, Mitsuru Royal Society of Chemistry 2010 Chemical communications Vol.46 No.11
<P>Well-aligned nanopatterns of various serum, antithrombogenic and cell adhesive proteins, such as γ-globulin, fibrinogen, thrombomodulin, fibronectin and type I collagen, were fabricated on a highly-oriented block copolymer lamellar surface, and these bioactive protein nanoarrays will be useful in biological research.</P> <P>Graphic Abstract</P><P>Unidirectional protein nanoarrays were fabricated on a highly-aligned block copolymer lamellar surface, and these bioactive protein nanoarrays will be useful in biological assays. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=b920068a'> </P>
Hiroki Tanaka,Shimpei Matsusaki,Youichirou Baba,Yoshiaki Isono,Tomohiro Sase,Hiroshi Okano,Tomonori Saito,Katsumi Mukai,Tetsuya Murata,Hiroki Taoka 대한소화기내시경학회 2018 Clinical Endoscopy Vol.51 No.2
Background/Aims: It is sometimes difficult to distinguish between malignant and benign biliary strictures using imaging studiesalone, and pathological diagnosis is necessary. The aim of this study was to determine the usefulness of endoscopic transpapillary tissuesampling and factors predictive of diagnostic accuracy. Methods: From April 2008 to December 2014, 136 patients underwent endoscopic transpapillary tissue sampling for malignantbiliary strictures. The cytological and histological findings were reported as negative, suspicious, or positive. Suspicious and positivefindings were defined as pathologically positive. Results: The sensitivity was 65.0% for forceps biopsy, 49.5% for brush cytology, 46.2% for bile aspiration cytology, and 21.9% forendoscopic nasobiliary drainage cytology. The combination of these procedures improved the sensitivity (72.8%). Endoscopictranspapillary tissue sampling was more sensitive for lesions of biliary origin (91.4%) than for extrabiliary lesions (66.3%). In surgicalcases, the sensitivity for tumors with an infiltrative growth pattern (53.3%) was significantly lower than for a tumor with an expandingor intermediate growth pattern (87.5%). Conclusions: Combining procedures can improve diagnostic accuracy. It may be possible to predict the sensitivity of endoscopictranspapillary tissue sampling by evaluating the etiology and tumor growth pattern using preoperative imaging studies.
A Detection of Airborne Particles Carrying Viable Bacteria in an Urban Atmosphere of Japan
Kazutaka Hara,Daizhou Zhang,Maromu Yamada,Hiromi Matsusaki,Koji Arizono 한국대기환경학회 2011 Asian Journal of Atmospheric Environment (AJAE) Vol.5 No.3
Viable bacteria on water-insoluble airborne particles were detected in the urban atmosphere of Kumamoto (134^ㅇ45′E, 32^ㅇ28′N), Japan, in autumn 2008. Airborne particles were collected onto film-covered Cu meshes under clear weather conditions. The samples were stained by fluorescent stains, and then viewed and photographed with an epifluorescent microscope. Non-biological and bacterial parts in particles larger than 0.8 μm were distinguished by their morphologies,fluorescent colors and fluorescent intensities. Bacterial viable statuses were discriminated according to cell membrane damage. In total, 2681 particles were investigated and it was found that 78 airborne particles were associated with bacteria. Viable bacteria were identified on 48 particles. A few particles carried multiple viable bacteria. These results provide the evidence that airborne particles act as carriers of viable bacteria in the atmosphere.
A Detection of Airborne Particles Carrying Viable Bacteria in an Urban Atmosphere of Japan
Hara, Kazutaka,Zhang, Daizhou,Yamada, Maromu,Matsusaki, Hiromi,Arizono, Koji Korean Society for Atmospheric Environment 2011 Asian Journal of Atmospheric Environment (AJAE) Vol.5 No.3
Viable bacteria on water-insoluble airborne particles were detected in the urban atmosphere of Kumamoto ($134^{\circ}45'E$, $32^{\circ}28'N$), Japan, in autumn 2008. Airborne particles were collected onto film-covered Cu meshes under clear weather conditions. The samples were stained by fluorescent stains, and then viewed and photographed with an epifluorescent microscope. Non-biological and bacterial parts in particles larger than 0.8 ${\mu}m$ were distinguished by their morphologies, fluorescent colors and fluorescent intensities. Bacterial viable statuses were discriminated according to cell membrane damage. In total, 2681 particles were investigated and it was found that 78 airborne particles were associated with bacteria. Viable bacteria were identified on 48 particles. A few particles carried multiple viable bacteria. These results provide the evidence that airborne particles act as carriers of viable bacteria in the atmosphere.