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RISS 인기검색어
- 검색키워드
- 저자 : Lovanh, Nanh (검색결과 3 건)
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Velu, Manikandan,Lee, Jeong-Ho,Chang, Woo-Suk,Lovanh, Nanh,Park, Yool-Jin,Jayanthi, Palaniyappan,Palanivel, Velmurugan,Oh, Byung-Taek Springer Berlin Heidelberg 2017 3 Biotech Vol.7 No.2
<P>Metal nanoparticles obtained from green route are gaining significant prominence as a result of their potential applications in nanomedicine and material engineering. Overall metal nanoparticles studied, silver nanoparticles (AgNPs) clutch prominent place in nanoparticles research field. Herein, we have reported the green synthesis of <I>Saccharum officinarum</I> leaf biomass extract-mediated synthesis of AgNPs. Initial nanoparticle production was confirmed by visual observation as color change from light yellow to bright brown color with yellow shade and spectrophotometrically at 450 nm and the various reaction conditions were optimized. The FTIR spectra of the biomass extract and synthesized AgNPs authorized the presence of phyto constituents as capping agent. The High Resolution-Transmission Electron Microscopy (HR-TEM) analyses confirm the morphology and the average particle size of AgNPs as ~28.2 nm. The crystalline nature oxide state and mean particle diameter of AgNPs were confirmed by X-ray diffraction (XRD) analysis, Selected Area Electron Diffraction (SAED) pattern and face-centered cubic (FCC). The obtained AgNPs show moderate to good antifungal activity against <I>Phytophthora capsici</I>, <I>Colletotrichum acutatum</I> and <I>Cladosporium fulvum</I> as 10, 12 and 14 mm zones of inhibition against synthesized AgNPs at 250 μg/well, respectively.</P>
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Lee, Jeong-Ho,Velmurugan, Palanivel,Park, Jung-Hee,Murugan, Kadarkarai,Lovanh, Nanh,Park, Yool-Jin,Oh, Byung-Taek,Venkatachalam, Perumal,Benelli, Giovanni Elsevier 2018 Physiological and molecular plant pathology Vol.101 No.-
<P><B>Abstract</B></P> <P>In this study, <I>Salvia miltiorrhiza-</I>synthesized Ag nanoparticles (AgNPs) fabricated using sunlight or various LED lights were studied for their biophysical features and evaluated as larvicides against <I>Aedes aegypti</I> mosquitoes and growth inhibitors on different species of microbial pathogens. AgNPs production post-exposure to sunlight or different LED light conditions (i.e. blue, red, green, and white) was confirmed by characteristic surface Plasmon resonance (SPR) at maximum λ of 430, 420, 460, 450, and 460 nm, respectively. Optimization of pH, reducing extract concentration, metal ion concentration and time elapsed from the nano-biosynthesis was achieved. High-resolution transmission electron microscopy (HR-TEM) showed that most AgNPs was spherical, triangular and oval, with average size of 18.5, 28.02, 50.22, 16.26 and 10.12 nm for white, green, red, blue and sunlight, respectively. XRD confirmed the all the obtained AgNPs showed face centered cubic (fcc) crystal lattice. FT-IR analysis of all synthesized AgNPs indicated the involvement of phenol, amine, hydroxyl and amino groups in the reduction of nano-Ag. All tested AgNPs inhibited the growth of <I>Brevibacterium linens</I> (KACC-14346), <I>Propionibacterium acnes</I> (KACC 11946), <I>Staphylococcus aureus</I> (KACC-10768) and <I>Staphylococcus epidermidis.</I> As a general trend, larvicidal assays conducted on dengue and Zika virus vector <I>Aedes aegypti</I> showed that, after 48 h of exposure, the toxicity achieved by sunlight-fabricated AgNPs was slightly higher if compared to AgNPs fabricated using various LED lights. Overall, our research highlighted the importance of abiotic parameters, with special reference to light condition, during green nanosynthesis of antimicrobials and larvicides.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A novel photo-biological engineering method for <I>Salvia miltiorrhiza-</I>mediated fabrication of silver nanoparticles using LED lights sources was established. </LI> <LI> Engineered biomolecule-capped silver nanoparticles were characterized by using FT-IR, XRD, HR-TEM analysis. </LI> <LI> Green synthesized nanoparticles exhibited enhanced antibacterial activity against microbial pathogens. </LI> <LI> Photo-fabricated metallic silver nanoparticles showed efficient larvicidal activity against dengue and Zika virus vector <I>Aedes aegypti.</I> </LI> </UL> </P>
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Palanivel Velmurugan,명현,Muthusamy Govarthanan,이영주,서상기,조광민,Nanh Lovanh,오병택 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.3
A total of five bacterial strains were isolated from earthworm, Eisenia fetida and examined for bacterial cellulose (BC) production in Hestrin–Schramm medium (HS). Among the five strains tested, CBNU-EW3 exhibited excellent BC production and was identified as Leifsonia sp. by 16S rDNA sequence analysis. BC production by Leifsonia sp. CBNU-EW3 was optimum at pH 5, 30°C, and with glucose and yeast extract as carbon and nitrogen sources, respectively, according to 15 day-long experiments. (XRD) analysis of the dried pellicle indicated that the BC was partially crystalline type I. Fourier transform infrared spectroscopy (FT-IR) analysis showed that the obtained pellicle contained the same functional groups as typical BC. Field emission scanning electron microscopy (FESEM) images showed that the BC micro-fibril matrix consisted of a flat surface with large pore size and cellulose aggregation.
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