Stress Model of Axial Flow Threshing & Separating Device and Trajectory Simulation of Rice Grains

Liu Ying-Nan,Yi Shu-Juan 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.11

To reveal stress distribution rules of grain separating process in assembled axial flow threshing and separating device, regarding assembled axial flow threshing and separating device as object, High speed imaging technology is adopted in the shooting technique, stress model was set based on the stress principle, and stress distribution rules was obtained by simulation technology. Grain, broken ear movement and the movement as a whole and free movement of the grain threshing process is through the analysis of the EXCEL drawing, The test results show that distribution rules is irregular in grain separating process, is that, stress fluctuates at axial direction, and two peak values appear at early and middle threshing stage respectively; threshing and separating process finishes about 2.35 meters from the feeding mouth at axial direction. Off the spike in threshing and separating space motion unstable trajectory is a parabola, the speed decreased with the passage of time. No outside interference in the most free grain for approximate straight movement, in the medium of motion state, and axis direction and a certain angle.Therefore, grain rotors distribute at axial direction and rest results are consistent with theory analysis.

Detection and Analysis of Residual Pesticides Based On Surface Raman Spectra of Spinach

Liu Ying-Nan,Yi Shu-Juan 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.12

This paper with spinach as the research object, to chlorpyrifos pesticide as detection object, using Raman spectroscopy technique surface pesticides in spinach residual detection method and rapid detection research. Starting from the detection method, study of the classic method and method for rapid detection of current situation of the development, Raman spectroscopy detection method as a means of detection, the selected object of study; master the Raman spectroscopy for rapid detection of the basic principles and originpro 9.0 software acquisition function using, lay the foundation for the subsequent detection of. After the actual test to determine Raman spectroscopy instrument laser wavelength to 473nm, acquisition time 2s spinach sample testing effect Best result. After no medicine Raman spectra to obtain the surface of spinach were denoising analysis results show that wavelet de noise method compared to the adjacent averaging, savitzky Golay method smoothing, median method effect better. On Spinach with different concentrations of chlorpyrifos Raman spectroscopy analysis that accorded with the residual linear relationship. The peak intensity method were selected to establish models were detected in spinach surface of pesticide chlorpyrifos. Raman spectroscopy detection method can of spinach surface of pesticide residues detection analysis. It solves the traditional measurement method for sample pretreatment is complex and long detection time, low efficiency of the measured defect

중국의 유휴산업시설을 복합문화공간으로 재생한 사례연구

유영남(Liu, Ying-Nan),주범(Chu, Beom) 대한건축학회 2021 대한건축학회 학술발표대회 논문집 Vol.41 No.1

Idle industrial facilities have some historical value and high availability of space, and can be recreated as complex cultural space by maximizing the preservation of historical buildings. In addition, the development of cultural industries led by the government and art and culture brought about by the influx of artists" studios has promoted the development of regional cultural industries and surrounding backward areas. This paper takes five compound cultural space architecture that has been successfully regenerated among the three fastest developing cities in China as an example.Analyzing the Spatial Composition after Regeneration. This paper provides reference for the regeneration of idle industrial facilities in other regions from the perspective of practice.

Vegetation Cover Change and Its Responses to Climate Change in the Changbai Mountain Area

NAN Ying(남영),LIU Zhi-feng 한국지역지리학회 2010 한국지역지리학회 학술대회 Vol.2010 No.1

Feasibility Study on Long-Term Continuous Ethanol Production from Cassava Supernatant by Immobilized Yeast Cells in Packed Bed Reactor

Liu Qingguo,Zhao Nan,Zou Yanan,Ying Hanjie,Dong Liu,Chen Yong 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.8

In this study, yeast cell immobilization was carried out in a packed bed reactor (PBR) to investigate the effects of the volumetric capacity of carriers as well as the different fermentation modes on fuel ethanol production. An optimal volumetric capacity of 10 g/l was found to obtain a high cell concentration. The productivity of immobilized cell fermentation was 16% higher than that of suspended-cell fermentation in batch and it reached a higher value of 4.28 g/l/h in repeated batches. Additionally, using this method, the ethanol yield (95.88%) was found to be higher than that of other tested methods due to low concentrations of residual sugars and free cells. Continuous ethanol production using four bioreactors showed a higher productivity (9.57 g/l/h) and yield (96.96%) with an ethanol concentration of 104.65 g/l obtained from 219.42 g/l of initial total sugar at a dilution rate of 0.092 h-1. Furthermore, we reversed the substrate-feed flow directions in the in-series bioreactors to keep the cells at their highest activity and to extend the length of continuous fermentation. Our study demonstrates an effective method of ethanol production with a new immobilized approach, and that by switching the flow directions, traditional continuous fermentation can be greatly improved, which could have practical and broad implications in industrial applications.

Utilization of Potato Starch Processing Wastes to Produce Animal Feed with High Lysine Content

( Ying Li ),( Bing Nan Liu ),( Jin Zhu Song ),( Cheng Jiang ),( Qian Yang ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.2

This work aims to utilize wastes from the potato starch industry to produce single-cell protein (SCP) with high lysine content as animal feed. In this work, S-(2-aminoethyl)-L-cysteine hydrochloride-resistant Bacillus pumilus E1 was used to produce SCP with high lysine content, whereas Aspergillus niger was used to degrade cellulose biomass and Candida utilis was used to improve the smell and palatability of the feed. An orthogonal design was used to optimize the process of fermentation for maximal lysine content. The optimum fermentation conditions were as follows: temperature of 40°C, substrate concentration of 3%, and natural pH of about 7.0. For unsterilized potato starch wastes, the microbial communities in the fermentation process were determined by terminal restriction fragment length polymorphism analysis of bacterial 16S rRNA genes. Results showed that the dominant population was Bacillus sp. The protein quality as well as the amino acid profile of the final product was found to be significantly higher compared with the untreated waste product at day 0. Additionally, acute toxicity test showed that the SCP product was non-toxic, indicating that it can be used for commercial processing.

A heat shock protein 70 protects the green peach aphid (Myzus persicae) against high-temperature stress

Chen Nan,Tan Jia-Yu,Wang Ying,Qi Ming-Hui,Peng Jiang-Nan,Chen De-Xin,Liu Su,Li Mao-Ye 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.4

Heat shock proteins (HSPs) are encoded by Hsp genes and are important in insect tolerance to heat stress. The green peach aphid, Myzus persicae, is an important agricultural pest. The functions of Hsp genes in the thermal tolerance of M. persicae are unknown. This study identified an Hsp70 gene (MpHsp70a) and analyzed its role in protection against high-temperature stress. MpHsp70a encoded a protein consisting of 659 amino acid residues. The protein had three signature motifs of the HSP70 family and was predicted to be localized in the cytoplasm. The highest expression level of MpHsp70a was in adults, and differences in the mRNA levels between apterous and alate adults were not significant. Exposure to high temperatures (30, 35 and 40 ◦ C) for one hour and treatment with 40 ◦ C for different times (0.5, 1 and 2 h) all resulted in a greatly elevated expression level of MpHsp70a, suggesting that the gene is heat-inducible. The transcriptional level of MpHsp70a was suppressed by injection with double-stranded RNA (dsRNA), and knockdown of MpHsp70a significantly increased the suscep tibility of apterous adults to 40 ◦ C. These results indicate that MpHsp70a is required for tolerance to hightemperature stress in M. persicae. Our findings highlight the molecular mechanism underlying Hsp70-mediated thermal adaptation in M. persicae.

Ginsenoside Rk1 ameliorates paracetamol-induced hepatotoxicity in mice through inhibition of inflammation, oxidative stress, nitrative stress and apoptosis

Jun-Nan Hu,Xing-Yue Xu,Wei Li,Yi-Ming Wang,Ying Liu,Zi Wang,Ying-Ping Wang 고려인삼학회 2019 Journal of Ginseng Research Vol.43 No.1

Background: Frequent overdose of paracetamol (APAP) has become the major cause of acute liver injury. The present study was designed to evaluate the potential protective effects of ginsenoside Rk1 on APAPinduced hepatotoxicity and investigate the underlying mechanisms for the first time. Methods: Micewere treated with Rk1 (10 mg/kg or 20 mg/kg) by oral gavage once per d for 7 d. On the 7th d, all mice treatedwith250mg/kgAPAPexhibitedsevere liver injuryafter24h, andhepatotoxicitywas assessed. Results: Our results showed that pretreatment with Rk1 significantly decreased the levels of serum alanine aminotransferase, aspartate aminotransferase, tumor necrosis factor, and interleukin-1b compared with the APAPgroup.Meanwhile, hepatic antioxidants, including superoxide dismutase andglutathione,were elevated compared with the APAP group. In contrast, a significant decrease in levels of the lipid peroxidation product malondialdehyde was observed in the ginsenoside Rk1-treated group compared with the APAP group. These effectswere associatedwith a significant increase of cytochromeP450 E1 and 4-hydroxynonenal levels in liver tissues. Moreover, ginsenoside Rk1 supplementation suppressed activation of apoptotic pathways by increasing Bcl-2 and decreasing Bax protein expression levels, which was shown using western blotting analysis.Histopathological observation also revealed that ginsenoside Rk1 pretreatment significantly reversed APAP-induced necrosis and inflammatory infiltration in liver tissues. Biological indicators of nitrative stress, such as 3-nitrotyrosine, were also inhibited after pretreatment with Rk1 compared with the APAP group. Conclusion: The results clearly suggest that the underlying molecular mechanisms in the hepatoprotection of ginsenoside Rk1 in APAP-induced hepatotoxicity may be due to its antioxidation, antiapoptosis, anti-inflammation, and antinitrative effects.

Ginsenoside Rk1 ameliorates paracetamol-induced hepatotoxicity in mice through inhibition of inflammation, oxidative stress, nitrative stress and apoptosis

Hu, Jun-Nan,Xu, Xing-Yue,Li, Wei,Wang, Yi-Ming,Liu, Ying,Wang, Zi,Wang, Ying-Ping The Korean Society of Ginseng 2019 Journal of Ginseng Research Vol.43 No.1

Background: Frequent overdose of paracetamol (APAP) has become the major cause of acute liver injury. The present study was designed to evaluate the potential protective effects of ginsenoside Rk1 on APAP-induced hepatotoxicity and investigate the underlying mechanisms for the first time. Methods: Mice were treated with Rk1 (10 mg/kg or 20 mg/kg) by oral gavage once per d for 7 d. On the 7th d, allmice treated with 250mg/kg APAP exhibited severeliverinjury after 24 h, and hepatotoxicitywas assessed. Results: Our results showed that pretreatment with Rk1 significantly decreased the levels of serum alanine aminotransferase, aspartate aminotransferase, tumor necrosis factor, and interleukin-$1{\beta}$ compared with the APAP group. Meanwhile, hepatic antioxidants, including superoxide dismutase and glutathione, were elevated compared with the APAP group. In contrast, a significant decrease in levels of the lipid peroxidation product malondialdehyde was observed in the ginsenoside Rk1-treated group compared with the APAP group. These effects were associated with a significant increase of cytochrome P450 E1 and 4-hydroxynonenal levels in liver tissues. Moreover, ginsenoside Rk1 supplementation suppressed activation of apoptotic pathways by increasing Bcl-2 and decreasing Bax protein expression levels, which was shown using western blotting analysis. Histopathological observation also revealed that ginsenoside Rk1 pretreatment significantly reversed APAP-induced necrosis and inflammatory infiltration in liver tissues. Biological indicators of nitrative stress, such as 3-nitrotyrosine, were also inhibited after pretreatment with Rk1 compared with the APAP group. Conclusion: The results clearly suggest that the underlying molecular mechanisms in the hepatoprotection of ginsenoside Rk1 in APAP-induced hepatotoxicity may be due to its antioxidation, antiapoptosis, anti-inflammation, and antinitrative effects.

TNF Polymorphisms and Cancer

Feng, Ren-Nan,Liu, Gui-You,Wang, Cheng,Sun, Chang-Hao,Li, Ying Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.6