AITC induces MRP1 expression by protecting against CS/CSE-mediated DJ-1 protein degradation via activation of the DJ-1/Nrf2 axis

Lingling Xu,Jie Wu,Nini Li,Chengjun Jiang,Yan Guo,Peng Cao,Dianlei Wang 대한생리학회-대한약리학회 2020 The Korean Journal of Physiology & Pharmacology Vol.24 No.6

The present study aimed to examine the effect of allyl isothiocyanate (AITC) on chronic obstructive pulmonary disease and to investigate whether upregulation of multidrug resistance-associated protein 1 (MRP1) associated with the activation of the PARK7 (DJ-1)/nuclear factor erythroid 2-related factor 2 (Nrf2) axis. Lung function indexes and histopathological changes in mice were assessed by lung function detection and H&E staining. The expression levels of Nrf2, MRP1, heme oxygenase-1 (HO-1), and DJ-1 were determined by immunohistochemistry, Western blotting and reverse transcription-quantitative polymerase chain reaction. Next, the expression of DJ-1 in human bronchial epithelial (16HBE) cells was silenced by siRNA, and the effect of DJ-1 expression level on cigarette smoke extract (CSE)-stimulated protein degradation and AITC-induced protein expression was examined. The expression of DJ-1, Nrf2, HO-1, and MRP1 was significantly decreased in the wild type model group, while the expression of each protein was significantly increased after administration of AITC. Silencing the expression of DJ-1 in 16HBE cells accelerated CSE-induced protein degradation, and significantly attenuated the AITC-induced mRNA and protein expression of Nrf2 and MRP1. The present study describes a novel mechanism by which AITC induces MRP1 expression by protecting against CS/CSEmediated DJ-1 protein degradation via activation of the DJ-1/Nrf2 axis.

Ensemble learning-based hierarchical retrieval of similar cases for site planning

Wang Pengyue,Guo Maozu,Han Yunsong,Zhao Lingling,Zhou Xiaoping,Zhang Dayu 한국CDE학회 2021 Journal of computational design and engineering Vol.8 No.6

Site planning not only involves the arrangement of a large number of elements but also considers the complexity of urban systems; hence, the design process involves large workloads and is time consuming. Retrieving similar existing cases helps architects optimize or accelerate the design process. This paper proposes a computational approach that provides valuable references by retrieving similar cases. Three types of attributes are extracted to represent a given parcel: land-use attributes, geometric attributes (area, orientation, etc.), and environmental attributes (greening rate, surrounding facilities, etc.). The complete hierarchical retrieval process is divided into three phases. The first phase selects cases whose land-use attributes are consistent with the target parcel. Then, the similarity distances between the given target parcel and the selected cases are calculated using geometric attributes. The eXtreme Gradient Boosting (XGBoost) classifier is adopted to determine which case is similar to the target parcel. Finally, similarity scores of the retrieved cases are calculated based on the environmental attributes to provide more options during the actual design. In total, 1189 cases with different land-use types in Beijing were collected for the case base. The comparative experimental results confirmed that the proposed ensemble learning-based hierarchical retrieval of similar cases approach improves the accuracy of retrieval results. Furthermore, we use a real-world target parcel to demonstrate the superiority and flexibility of the retrieval process.

A Systematic Review of Network Flow Watermarking In Anonymity Systems

Tianbo Lu,Rui Guo,Lingling Zhao,Yang Li 보안공학연구지원센터 2016 International Journal of Security and Its Applicat Vol.10 No.3

With the rapid development of internet technology, the connection between man and internet is closer and closer. When people is communicating with others through internet, some malicious intruders may want to eavesdrop or peep the communicators. In order to evade being watching, people use anonymous communication systems to communicate. The anonymity system can encrypt the content of communication and the identity of the communicators. But if the communicators want to know who is talking to them at the other end, they must correlate the outgoing and incoming flows to identify a host or a person. As an active traffic analysis approach, network flow watermarking technology can detect the correlation of flows, and then make the anonymous communicators accountable. While network flow watermarking technology achieves good detecting rate and low false positive rate, it could be an effective way to trace the communication connections and supervise anonymous communication. So it is a widely used way for tracing in anonymity systems. In this paper, we introduce some different schemes of network flow watermarking in anonymity systems and discuss some attacks against it. Finally, a conclusion will be given.

Security Model for Sensitive Information Systems and Its Applications in Sensor Networks

Tianbo Lu,Xiaobo Guo,Lingling Zhao,Yang Li,Peng Lin,Binxing Fang 보안공학연구지원센터 2015 International Journal of Security and Its Applicat Vol.9 No.5

The study of security models for sensitive information systems has been taken on for years, but still lag far away behind the progress of information security practice. During this century, the thought of seeking the system security to the source of system development lifecycle received huge improvement in the system and software assurance domain. This paper firstly expounds the understanding of information security by illustrating information security study development progress since pre-computer age and presents a description of cyberspace and cyberization security by summarizing the status quo of cyberization. Then a security model called PDRL, which includes six core security attributes of sensitive information systems, is proposed to protect the security of sensitive information systems in the whole system life-cycle. At last, this paper probes into further discussion about controllability attribute and proposes a controllability model in sensitive sensor networks, followed by a probability computing formula and the example for computing the controllability of sensitive sensor networks. By dividing each single element of sensitive information and each element-related operation into a corresponding classification, this paper makes a reasonable description of the quantitative description about controllability.

Zhizhu Decoction Promotes Gastric Emptying and Protects the Gastric Mucosa

Wei He,Yi Zhang,Xinrui Wang,Lingling Guo,Lifeng Han,Erwei Liu,Tao Wang 한국식품영양과학회 2013 Journal of medicinal food Vol.16 No.4

The aim of this study was to evaluate the effects of the Zhizhu decoction on gastric emptying and gastric mucosal protection. The Zhizhu decoction is composed of Aurantii fructus and Atractylodes macrocephala Rhizoma. Results showed that oral administration of the Zhizhu decoction accelerated gastric emptying in mouse and protected gastric mucosa from ethanol-induced ulcers in rat. Our investigations demonstrated that the Zhizhu decoction accelerated gastric emptying, at least in part, by activating the muscarinic and 5-HT3 receptors. The gastroprotective effect is involved in its antioxidant effects and increased vascular endothelial growth factor expression.

Eco-friendly Polypyrrole-coated Cocozelle Composites for Supercapacitor Application

Hui Ji,Chuanjie Zhang,Weida Rao,Bin Guo,Lingling Fan,Zikui Bai,Haifeng Bao,Jie Xu 한국섬유공학회 2020 Fibers and polymers Vol.21 No.6

Cocozelle is essentially consisted of hemicellulose and lignin with a hierarchically porous structure. Herein,cocozelle slices after freeze-drying were employed as green substrates to support the growth of polypyrrole (PPy) via a facileoxidative polymerization approach. The cocozelle surface was densely covered with sheet-like PPy due to the hydrogenbonding between PPy and cocozelle. The PPy/cocozelle composites could be facilely utilized as supercapacitor electrodes,which showed a high specific capacitance of 1108 F g-1 with an energy density of 70.5 Wh kg-1 and a power density of398 W kg-1 at a charge/discharge current density of 0.8 mA cm-2 as well as desirable cycling life with a capacitance retentionrate of 76.9 % after 1000 cycles. Moreover, 95.3 % of the initial specific capacitance obtained at 0.2 mA cm-2 could bereached as the current density increased to 1.6 mA cm-2. These results demonstrate that cocozelle could be used as a superioralternative biomass-derived substrate for the developments of eco-friendly and renewable energy storage devices.

Succession of the Nanoplankton Community during a Brown Tide in a Scallop Culture Area in China

Ling Qiao,Jie Yu,Ying Li,Haobing Guo,Yu Zhen,Lingling Zhang,Tiezhu Mi,Fuchong Zhang,Zhenmin Bao 한국해양과학기술원 2019 Ocean science journal Vol.54 No.3

Brown tides caused by Aureococcus anophagefferens have occurred frequently in the coastal waters of Qinhuangdao since 2009. The Changli coast is the area most strongly affected by the brown tides, which result in significant economic losses and serious ecological harm. To understand the effects of brown tides on nanoplankton diversity and biomass, samples were collected from May to July in 2012 at station XKK, which is approximately 0.2 nautical miles off the Changli coast. The V9 region of the 18S rDNA gene was used as a marker and was analyzed by Illumina sequencing to assess the nanoplankton diversity and biomass, and real-time fluorescence quantitative PCR was performed to analyze the temporal variations in the copy concentrations of the 18S rDNA of nanoplankton. The results showed that nanoplankton diversity, as determined by the Shannon index, decreased from May to June and increased in July. Illumina sequencing analysis revealed that the plankton community differed among the samples from May to July, and over this period, the dominant phytoplankton groups changed from pelagophytes to chlorophytes. Sufficient nutrients and low grazing pressure by zooplankton and scallops accelerated the growth of small-celled phytoplankton. A. anophagefferens was the most dominant phytoplankton species, with an 18S rDNA maximum copy concentration detected on 26 June, followed by Micromonas sp. and Bathycoccus prasinos, with maximum copy concentrations observed on 3 July. Ciliophora, Arthropoda and Cercozoa were the dominant taxa of the local zooplankton community. The Ciliophora and Arthropoda biomasses decreased, and the Cercozoa biomass increased when the copy concentration of A. anophagefferens peaked on 26 June. We suspect that A. anophagefferens had a significantly negative effect on the growth of Ciliophora and Arthropoda and could be ingested by Cercozoa to promote its proliferation. The small and low-abundance plankton that are likely missed in microscopic and clone library analyses could be detected using Illumina sequencing and qPCR, which provide an effective method for a comprehensive analysis of the plankton community.