Correlation between DNA methylation and Thymic Stromal Lymphopoietin expression in asthmatic airway epithelial cells

Yan‑Li Li,Xi‑Qian Xing,Yi Xiao,Yan‑Hong Liu,Yu‑Shan Zhou,Min Zhuang,Chao‑Qian Li 한국유전학회 2020 Genes & Genomics Vol.42 No.12

Background: The overexpression of TSLP and DNA methylation in asthma were both risk factors the relationship was not clear. Objective: This study aimed to investigate the relationship between methylation status of TSLP promoter and mRNA/protein expression in asthmatic airway epithelial cells. Methods: Human bronchial epithelial cells were cultured in vitro and divided into: Control group, treated with PBS, model group, sensitized with LPS (10 μg/mL) for 12 h (37 °C, 5% CO2). Other groups were cultured with the pCMV3 plasmid (M + NC/pCMV), pGPH1 plasmid (M + NC/pGPH), DNMT1/pCMV3 plasmid (M + DNMT1/pCMV), and DNMT1/pGPH1 plasmid (M + DNMT1/pGPH) for 48 h. The expression of DNA methyltransferase 1 and TSLP were measured using real-time PCR and western blotting. Results: Compared with the control group, TSLP mRNA (1.00 ± 0.00 vs. 2.82 ± 0.81 vs. 1, P < 0.001) and protein (1.07 ± 0.04 vs. 1.46 ± 0.11, P < 0.01) were significantly greater, and the methylation of promoter was lower (92.75 ± 1.26 vs. 58.57 ± 3.34, P < 0.05) in the model group. Compared with the model group, TSLP mRNA (2.82 ± 0.81 vs. 1.17 ± 0.10, P < 0.001) decreased, but TSLP promoter methylation increased (58.57 ± 3.34 vs. 92.58 ± 7.30, P < 0.05) in M + DNMT1/pCMV. TSLP mRNA and protein were higher (2.82 ± 0.81 vs. 5.32 ± 0.21, P < 0.001; 1.46 ± 0.11 vs. 1.94 ± 0.11, respectively, P < 0.01), TSLP promoter methylation was lower (58.57 ± 3.34 vs. 33.57 ± 4.29, P < 0.05) in M + DNMT1/pGPH. Conclusions: Overexpression of TSLP in asthmatic airway epithelial cells may be regulated by DNA demethylation.

Improved lithium storage performance of CeO2-decorated SrLi2Ti6O14 material as an anode for Li-ion battery

Ying Li,Hong-Yan Liu,Ling-Na Shi,Yan-Rong Zhu,Ting-Feng Yi 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.101 No.-

In this work, the CeO2-decorated SrLi2Ti6O14 anode was successfully prepared through a solid-state process. The space-charge effect induced by the internal adsorption of ions on the CeO2 surface can easilyresult in a formation of an excellent conductive interfacial layer between CeO2 and SrLi2Ti6O14. The goodelectrical contact between CeO2 and SrLi2Ti6O14 offers more active sites for the electrolyte storage andredox reaction, and promotes the intercalation/deintercalation of lithium ions, and thus improves therate performance and cycle stability. Due to its unique structure and composition, the CeO2-decoratedSrLi2Ti6O14 composites exhibit high reversible capacities, good cycle performance and outstanding rateperformance. Especially, the CeO2 (5 wt%)-decorated SrLi2Ti6O14 anode shows the most excellent electrochemicalperformance, which delivers a large charge capacity of 121.3 mAh g 1 and a capacity retentionof 94.48% after 100 cycles at 0.5 A g 1. However, the corresponding charge capacity and capacity retentionof pristine SrLi2Ti6O14 are 100.5 mAh g 1 and 86.77%, respectively. The CeO2(5 wt%)-decoratedSrLi2Ti6O14 with enhanced rate capacity, cycle stability and structural stability is a potential electrodematerial candidate for Li-ion battery.

Preparation of lithium-doped NaV6O15 thin film cathodes with high cycling performance in SIBs

Xu Hai-Yan,Ruan Jun Hai,Liu Fang Lin,Li Dong-Cai,Zhang Feng-Jun,Wang Ai-Guo,Sun Dao-Sheng,오원춘 한국세라믹학회 2022 한국세라믹학회지 Vol.59 No.3

Lithium ions-doped NaV6O15 thin films have been prepared using a simple low temperature liquid phase deposition method and subsequent annealing process. X-ray diffraction (XRD), Fourier transform infrared spectrometer (FTIR), scanning elec- tron microscopy (SEM), and photoelectron spectroscopy (XPS) have been used to study the structural and physicochemical characteristics of the NaV6O15 film. The films were grown on the FTO conductive glass and used directly as an electrode of sodium ion batteries. The prepared lithium ions-doped NaV6O15 thin film electrodes showed an excellent cycling stability and discharge capacity, which may be attributed to the stability of the Li+ embedded into the gap between the V–O layers to maintain the structure and its stable β-phase structure transformed after the first cycle. The cycling stability greatly improved with increasing annealing temperature, while the discharge capacity decreased. The capacities of the film electrodes annealed at 400 °C and 450 °C maintained above 97% after 100 cycles. The lithium-doped NaV6O15 underwent a phase transition dur- ing the first charge/discharge cycle. The new transformed phase has perfect crystal structure stability undergoing insertion and deinsertion of Na+. Therefore, the lithium-doped NaV6O15 thin film possesses good cycling stability and is expected to be a promising thin film cathode for sodium-ion batteries.

Well-designed Te/SnS₂/Ag artificial nanoleaves for enabling and enhancing visible-light driven overall splitting of pure water

Yan, Changzeng,Xue, Xiaolan,Zhang, Wenjun,Li, Xiaojie,Liu, Juan,Yang, Songyuan,Hu, Yi,Chen, Renpeng,Yan, Yaping,Zhu, Guoyin,Kang, Zhenhui,Kang, Dae Joon,Liu, Jie,Jin, Zhong unknown 2017 Nano energy Vol.39 No.-

<P><B>Abstract</B></P> <P>To produce hydrogen and oxygen from photocatalytic overall splitting of pure water provides a promising green route to directly convert solar energy to clean fuel. However, the design and fabrication of high-efficiency photocatalyst is challenging. Here we present that by connecting different nanostructures together in a rational fashion, components that cannot individually split water into H<SUB>2</SUB> and O<SUB>2</SUB> can work together as efficient photocatalyst with high solar-to-hydrogen (STH) energy conversion efficiency and avoid the use of any sacrificial reagent. Specifically, Te/SnS<SUB>2</SUB>/Ag artificial nanoleaves (ANLs) consist of ultrathin SnS<SUB>2</SUB> nanoplates grown on Te nanowires and decorated with numerous Ag nanoparticles. The appropriate band structure of Te/SnS<SUB>2</SUB> p-n junctions and the surface plasmon resonance of Ag nanoparticles synergistically enhance the quantum yield and separation efficiency of electron-hole pairs. As a result, Te/SnS<SUB>2</SUB>/Ag ANLs enable visible-light driven overall water-splitting without any sacrificial reagent and exhibit high H<SUB>2</SUB> and O<SUB>2</SUB> production rates of 332.4 and 166.2μmolh<SUP>−1</SUP>, respectively. Well-preserved structure after long-term measurement indicates its high stability. It represents a feasible approach for direct H<SUB>2</SUB> production from only sunlight, pure water, and rationally-designed ANL photocatalysts.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Te/SnS<SUB>2</SUB>/Ag ANLs heterostructure is prepared to catalyze overall water splitting. </LI> <LI> The catalyst show impressive H<SUB>2</SUB> and O<SUB>2</SUB> production rate under visible light. </LI> <LI> The structure and efficiency of catalyst shows no degradation after 10 days. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Extraction of visual texture features of seabed sediments using an SVDD approach

Li, Yan,Liu, Shijie,Zhu, Puqiang,Yu, Jiancheng,Li, Shuo Pergamon Press 2017 Ocean engineering Vol.142 No.-

<P><B>Abstract</B></P> <P>Perception of the seabed environment is an important capability of autonomous underwater vehicles. This paper focuses on defining and extracting robust texture features from visual images that lead to useful and practical automated identification of the types of seabed sediments. The visual texture features are described by using a gray-level co-occurrence matrix (GLCM) and fractal dimension, after which an unsupervised learning method, self-organizing map (SOM), is adopted to evaluate the validity of features descriptors on three types of seabed sediments. Subsequently, a kernel-based approach that exhibits robustness versus low numbers of high-dimensional samples, named support vector domain description (SVDD), is applied to classify the types of seabed sediments. In comparison with state-of-the-art classifiers, the experimental results demonstrated the effectiveness of the SVDD on the classification of seabed sediments.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The visual images of seabed sediments are characterized by the texture features which are extracted based on the GLCM and fractal theory. </LI> <LI> A multi-class classification strategy for seabed sediments is proposed by adding a distance measure after SVDD implementation. </LI> <LI> The experimental results demonstrate that the proposed classification strategy is feasible in recognizing the type of seabed sediments. </LI> </UL> </P>

Vitamin D status after colorectal cancer diagnosis and patient survival according to immune response to tumour

Hamada, Tsuyoshi,Liu, Li,Nowak, Jonathan A.,Mima, Kosuke,Cao, Yin,Ng, Kimmie,Twombly, Tyler S.,Song, Mingyang,Jung, Seungyoun,Dou, Ruoxu,Masugi, Yohei,Kosumi, Keisuke,Shi, Yan,da Silva, Annacarolina,G Elsevier 2018 European journal of cancer Vol.103 No.-

<P><B>Abstract</B></P> <P><B>Background</B></P> <P>High-level plasma 25-hydroxyvitamin D [25(OH)D] has been associated with lower colorectal cancer incidence and mortality. Considering evidence indicating immunomodulatory effects of vitamin D, we hypothesised that survival benefits from high systemic vitamin D level might be stronger for colorectal carcinoma with lower immune response to tumour.</P> <P><B>Methods</B></P> <P>Using 869 colon and rectal cancer cases within the Nurses' Health Study and Health Professionals Follow-up Study, we assessed the prognostic association of postdiagnosis 25(OH)D score [derived from diet and lifestyle variables to predict plasma 25(OH)D level] in strata of levels of histopathologic lymphocytic reaction. The Cox proportional hazards regression model was adjusted for potential confounders, including microsatellite instability, CpG island methylator phenotype, LINE-1 methylation, <I>PTGS2</I> (cyclooxygenase-2) expression and <I>KRAS</I>, <I>BRAF</I> and <I>PIK3CA</I> mutations.</P> <P><B>Results</B></P> <P>The association of postdiagnosis 25(OH)D score with colorectal cancer-specific mortality differed by levels of peritumoural lymphocytic reaction (<I>p</I> <SUB>interaction</SUB> = 0.001). Multivariable-adjusted mortality hazard ratios for a quintile-unit increase of 25(OH)D score were 0.69 [95% confidence interval (CI), 0.54–0.89] in cases with negative/low peritumoural lymphocytic reaction, 1.08 (95% CI, 0.93–1.26) in cases with intermediate peritumoural reaction and 1.25 (95% CI, 0.75–2.09) in cases with high peritumoural reaction. The survival association of the 25(OH)D score did not significantly differ by Crohn's-like lymphoid reaction, intratumoural periglandular reaction or tumour-infiltrating lymphocytes.</P> <P><B>Conclusions</B></P> <P>The association between the 25(OH)D score and colorectal cancer survival is stronger for carcinomas with lower peritumoural lymphocytic reaction. Our results suggesting interactive effects of vitamin D and immune response may contribute to personalised dietary and lifestyle intervention strategies.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The survival association of vitamin D is stronger in cancer with fewer lymphocytes. </LI> <LI> Vitamin D and peritumoural lymphocytes interact to modify cancer progression. </LI> <LI> Vitamin D may exert anti-tumour immune-enhancing effects. </LI> <LI> Peritumoural lymphocytic reaction may be a biomarker for benefits from vitamin D. </LI> <LI> Our data support immunomodulatory lifestyle interventions for cancer patients. </LI> </UL> </P>

Impact of high-temperature-water cooling damage on the mechanical properties of concrete

Zhai, Yue,Li, Yan,Li, Yubai,Wang, Siwei,Liu, Yi,Song, Ki-Il Elsevier 2019 Construction & building materials Vol.215 No.-

<P><B>Abstract</B></P> <P>A series of impact tests were conducted on split Hopkinson pressure bar equipment to study the influence of high-temperature-cooling damage on the mechanical properties of concrete. The experimental investigation showed the influence on the peak stress, peak strain, and fracture mode. The results indicate that the strain rate is directly related to the loading rate and heating temperature. The test results of natural cooling and water immersion cooling treatment were compared, which showed that the strength of water-cooled specimens is higher than that of naturally cooled specimens before the heating temperature reaches 400 °C and becomes much lower when the heating temperature is higher than 400 °C. Based on the experimental study, a dynamic non-linear elastic constitutive equation was established using a particle swarm optimization algorithm and an inversion analysis method. These results could provide theoretical references for the design of the ultimate strength of concrete materials for practical applications, such as fire and explosion prevention.</P> <P><B>Highlights</B></P> <P> <UL> <LI> High temperature-cooling damage on mechanical properties for concrete is tested. </LI> <LI> The strain rate is directly related to the loading rate and heating temperature. </LI> <LI> 400 °C can be considered as the threshold of high temperature-water cooling damage. </LI> <LI> Dynamic non-linear elastic constitutive equation is established. </LI> </UL> </P>

Applicability Analysis of Microseismic Technology in Tunnel Water Inrush Monitoring

Yan-hao Chen,Shuai Cheng,Li-Ping Li,Jun-yan Yang,Hong-liang Liu,Wen-feng Tu 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.7

Water inrush disaster is one of the most serious geological disasters in tunnel construction. There are still some problems in the existing researches, such as the activity information of surrounding rock is difficult to obtain, the mechanism of water inrush disaster is unclear, the correlation between water inrush disaster and microseismic (MS) information is missing, which have caused the water inrush disaster to be passively controlled. Combining with the practical experience of tunnel engineering, the application problems of MS technology in tunnel engineering is summarized, which provides ideas for monitoring of surrounding rock activity. The formation mechanism of water inrush disaster caused by rock mass fracturing and filling medium instability has been revealed. The MS characteristics of the water inrush channel in different water inrush disasters have also been revealed. The MS response characteristics of water inrush and rockburst disasters are compared, which provides guidance for the improvement of MS monitoring technology and the analysis of effective MS information. The compression failure test of limestone under saturated and natural conditions is carried out. The results show that the presence of water weakens the ability of rock to store energy. The acoustic emission (AE) phenomenon is weaker in the process of saturated limestone failure, which increases the difficulty of AE monitoring. The number of AE events of natural limestone and saturated limestone shows a slow increase to a sudden increase in the process stress loading, which can be regarded as an important precursor of rock failure.

Cloning and characterization of hemerythrin gene from Sipuncula Phascolosoma esculenta

Yan Liu,Chenghua Li,Xiurong Su,Mengqian Wang,Yanyan Li,Ye Li,Taiwu Li 한국유전학회 2013 Genes & Genomics Vol.35 No.1

Hemerythrin is a non-heme respiratory protein involved in oxygen storage and transport in invertebrates. In the present study, the hemerythrin cDNA was cloned from Phascolosoma esculenta (denoted as PeHr) by PCR and rapid amplification of cDNA ends approaches. The full-length PeHr consisted of 770 bp containing of a 50-terminal untranslated region (UTR) of 83 bp, a 30-terminal UTR of 327 bp, and a coding domain sequence of 360 bp encoding a polypeptide of 120 amino acids with estimated molecular mass of 13.6 kDa and theoretical isoelectric point of 5.78. The expression profiles of PeHr were evaluated by real-time RT-PCR under blood loss stress. The expression level of PeHr was significantly up-regulated from 45 to 48 h, then slightly recovered to its original level. The coding sequence of the PeHr was cloned and expressed in Escherichia coli BL21 (DE3) for antibodies preparation. Western blotting analysis conformed that the generated antibodies could specifically identify not only recombinant product, but also native protein from the total protein extraction. Our results indicated that PeHr might be involved into haemocytes regeneration, and its function roles should be further investigated by the generated antibodies.

No Association Between the USP7 Gene Polymorphisms and Colorectal Cancer in the Chinese Han Population

Li, Xin,Wang, Yang,Li, Xing-Wang,Liu, Bao-Cheng,Zhao, Qing-Zhu,Li, Wei-Dong,Chen, Shi-Qing,Huang, Xiao-Ye,Yang, Feng-Ping,Wang, Quan,Wang, Jin-Fen,Xiao, Yan-Zeng,Xu, Yi-Feng,Feng, Guo-Yin,Peng, Zhi-Ha Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

Colorectal cancer (CRC), now the third most common cancer across the world, is known to aggregate in families. USP7 is a very important protein with an important role in regulating the p53 pathway, which is critical for genomic stability and tumor suppression. We here genotyped eight SNPs within the USP7 gene and conducted a case-control study in 312 CRC patients and 270 healthy subjects in the Chinese Han population. No significant associations were found for any single SNP and CRC risk. Our data eliminate USP7 as a potential candidate gene towards for CRC in the Han Chinese population.