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Background and Aims: To explore the molecular mechanisms of miR-886-5p in breast cancer., we examined roles in inhibiting growth and migration of MCF-7 cells. Methods: MiR-886-5p mimics and inhibitors were used to express or inhibit MiR-886-5p, respectively, and MTT and clone formation assays were used to determine the survival and proliferation. Hoechst 33342/ PI double staining was applied to detect apoptosis. The expression of caspase-3, caspase-8, caspase-9, MT1-MMP, VEGF-C and VEGF-D was detected by Western blotting, and the levels of MMP2 and MMP9 secreted from MCF-7 cells were assessed by ELISA. MCF-7 cell migration was determined by wound healing and Transwell assays. Results: We found that the growth of MCF-7 cells was inhibited upon decreasing miR-886-5p levels. Inhibiting miR-866-5p also significantly induced apoptosis and decreased the migratory capacity of these cells. The expression of VEGF-C, VEGF-D, MT1-MMP, MMP2, and MMP9 was also found to be decreased as compared to controls. Conclusions: Our data show that downregulation of miR-886-5p expression in MCF-7 cells could significantly inhibit cell growth and migration. This might imply that inhibiting miR-886-5p could be a therapeutic strategy in breast cancer.
In this paper, we focus on the per-flow throughput analysis of IEEE 802.11 multi-hop ad hoc networks. The importance of an accurate saturation throughput model lies in establishing the theoretical foundation for effective protocol performance improvements. We argue that the challenge in modeling the per-flow throughput in IEEE 802.11 multi-hop ad hoc networks lies in the analysis of the freezing process and probability of collisions. We first classify collisions occurring in the whole transmission process into instantaneous collisions and persistent collisions. Then we present a four-dimensional Markov chain model based on the notion of the fixed length channel slot to model the Binary Exponential Backoff (BEB) algorithm performed by a tagged node. We further adopt a continuous time Markov model to analyze the freezing process. Through an iterative way, we derive the per-flow throughput of the network. Finally, we validate the accuracy of our model by comparing the analytical results with that obtained by simulations.
The morphologies and structures of Ni(OH)2–graphene hybrid materials were tailored by using different mineralizers in this work. It was revealed that the synergic effects of the highly oxidized graphene sheets and the mineralizers played a crucial role in controlling the morphology and structure of the nanocomposites, and Na2CO3 is a very effective mineralizer for growing chiseled 2D nanoplates of Ni(OH)2 on graphene sheets. When produced with NaOH, fragmental Ni(OH)2 crystals with irregular shapes erratically decorated on graphene sheets. In contrast, chiseled Ni(OH)2 hexagonal nanoplates grown on graphene sheets were obtained when Na2CO3 was used as the mineralizer. These unique 2D–2D nanoarchitectures with higher contact area between the nanocrystals and graphene substrate can increase the interfacial interaction and then efficiently improve the structural stability of the composite material, thus exhibiting an enhanced Li storage capacity and excellent cycling performance of 562 mAh g-1 after the 36th cycle.
Sittaung-Thanlwin region in Lower Myanmar is an ecological niche for human settlement. Evidences of human activities in the region are seen through various archaeological sites or settlements along the coastal area between the rivers Sittaung and Thanlwin (Salween). In Lower Myanmar, scholar, U Aung Myint, discovered one major site Kyaikkatha and other small scale sites, namely, Sittaung, Kawhtin, Kadaikgyi, Kadaikkalay, Katkadit, Kelatha (little Zothoke), Ayetthama, Winka, Zothoke (big Zothoke), Lagonbyi (Sampannago), Wagaru, Laming and Ye in present day Mon State. In 1980, U Aung Myint undertook an exploration program at Kyaikkatha. After the exploration, an excavation team conducted systematic digging at Kyaikkatha in March 1986. Excavation continued occasionally at Kyaikkatha throughout the years between 1995 and 2000. It is known that Kyaikkatha, the old city, reveals a kind of monumental civilization exposing four religious structures (a stupa and three monasteries). This essay looks into the distribution of features within Kyaikkatha and infers on its social, political, and religious organization. This essay is also about a new discovery of an early urban settlement located at the apex of the Gulf of Muttama (Martaban) where a lost city of Suva..ahumi flourished in the ancient days. By means of aerial photographic observations, the site had been noticed recently, which was by followed some excavations. More systematic and detail surveying may be needed to know more about the site and its features for comparison to other similar settlements of through-out mainland South East Asia.
A novel hyperbranched poly(aryl ether ketone) terminated with cobalt phthalocyanine (CoPc-T-HPAEK)was prepared using 1,3,5-tris[4-(4-fluorobenzoyl) phenoxy] benzene (B3), as a ‘core' molecule, 4,4'-(hexafluoroisopropylidene)diphenol (6F-BPA) as an A2 monomer, and cobalt phthalocyanine as an end-capping reagent. CoPc-THPAEK showed good solubility, thermal stability and catalytic activity in the oxidative decomposition of 2,4,6-trichlorophenol (TCP) under H2O2 or KHSO5. Moreover, the oxidative effect of KHSO5 was superior to that of H2O2,and the catalytic activity of CoPc-T-HPAEK was much higher than that of linear poly(aryl ether ketone) terminated with cobalt phthalocyanine (CoPc-T-LPAEK) and linear poly(aryl ether ketone) with a cobalt phthalocyanine pendant unit (CoPc-S-LPAEK).
'스콜라' 이용 시 소속기관이 구독 중이 아닌 경우, 오후 4시부터 익일 오전 7시까지 원문보기가 가능합니다.
This study generally analyses the pronunciation of Korean stops for Chinese speakers, comparing with Korean native speakers,in terms of acoustic and phonetic characteristics. Aiming at the reasons why Chinese speakers mispronounced Korean stops, this research creates an experimental comparison of stops located at two different positions in Korean words, word-initial and word-medial positions, produced by Chinese speakers and Korean native speakers respectively. The parameters used in this experiment, including the voice onset time for frication at both the word-initial and word-medial positions, starting pitch value of stops-following vowels, and closure duration in the word-medial position, are measured and computed by PRAAT. Statistically speaking, as the experiment is running between 66 Chinese speakers and 18 Korean native speakers, we can draw a relatively accurate conclusion: with all the stops that Chinese speakers produced, in terms of acoustic and phonetic features, the fortis /ㄲ, ㅃ, ㄸ/ are similar with those which phonated by Korean native speakers, however lax consonants /ㄱ, ㅂ, ㄷ/ and aspiration /ㅋ, ㅍ, ㅌ/ are not. To illustrate this observation, we figured that, in order to distinguish the difference between lax consonants and aspiration, Chinese speakers deliberately pronounce the lax consonants /ㄱ, ㅂ, ㄷ/ in a higher pitch value (even as high as the pitch value of aspiration pronounced by Korean native speakers). In addition, the starting pitch value of the vowel after /ㅂ, ㅃ, ㅍ/ is lower the rather that after /ㄱ, ㄲ, ㅋ/, /ㄷ, ㄸ, ㅌ/, thus we assume that Chinese speakers would mispronounce /ㅂ, ㅃ, ㅍ/ more. Based on the fact being discovered in the experiment, we figured that the mispronouncing phenomenon that we mentioned earlier accounts for the difference between Chinese and Korean phonological system. Generally speaking, there are two distinctive consonants instead of three in Mandarin stops system, therefore, most Chinese speakers tend to be more sensitive to the existence of aspirations, which leads to the mispronouncing.
<P>A phytochemical investigation of the flowers of Chrysanthemum indicum yielded sesquiterpenoids 1-25 with various carbocyclic skeletons, including 10 new (1-10) and 15 known (11-25) analogues. The structures were elucidated via their physical data, while the absolute configuration of compounds 6, 8, and 10 was assessed via electronic circular dichroism analysis. The evaluation of the effect of sesquiterpenoids on porcine epidemic diarrhea virus (PEDV) replication showed that compounds 1-5, 12, 14, 16, 17, 19, and 21 increased cell viability against cell death in PEDV-injected cells. Compounds 2, 12, and 17 were selected and investigated for their inhibition of proteins required for PEDV replication. Compounds 2 and 17 significantly reduced PEDV nucleocapsid and spike protein synthesis compared with azauridin as a positive control.</P>
'스콜라' 이용 시 소속기관이 구독 중이 아닌 경우, 오후 4시부터 익일 오전 7시까지 원문보기가 가능합니다.
This study generally analyses Chinese speakers' production of Korean affricates acoustically and phonetically, compared with Korean native speakers. Aiming at finding the reasons why Chinese speakers make pronouncing errors in a certain way, this essay creates an experimental comparison of the two different kinds of Korean affricates produced by Chinese speakers and Korean native speakers. In the experiment, the voice onset time of closure and frication at the word-initial position and pitch value of the following vowel are measured; at the wordmedial position, closure duration, voice onset time of closure and frication, and pitch value of the following vowel are also measured or computed. By contrasting the pronunciation of 45 Chinese speakers and 14 Korean native speakers, the conclusion was drawn as follow: in all the affricates that Chinese speakers produced, the characteristics of fortis /ᄍ/ and aspiration /ᄎ/ were similar with those which Korean native speakers phonated. However, when Chinese pronounced /ᄌ/ and /ᄎ/, there is tendency to have a high pitch value, which was distinguishing from Korean native speakers. The study assumed that errors were caused by the different phonological system. Since there are two distinctive consonants instead of three in Mandarin affricate system, Chinese speakers are tend to be more sensitive to the existence of aspiration, which is also the primary feature to recognize affricates when pronouncing them.