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K. Ganesh Kumar,P. Balaji Bhargav,Nafis Ahmed,C. Balaji 한국전기전자재료학회 2021 Transactions on Electrical and Electronic Material Vol.22 No.6
In the present study, pure and Tin (Sn) doped ZnO (Sn–ZnO) nanostructures are grown using hydrothermal method. The effect of Sn doping on the physical properties of ZnO is extensively studied. Till 4 mol% of Sn doping, the dopant is completely dissolved in the host matrix and no secondary phases are formed as evident from XRD studies. The presence of the constituent elements of the host matrix (Zn,O) and dopant (Sn) is confi rmed using Energy Dispersive X-ray Spectroscopy (EDX). From FESEM images, it is evident that the morphology of the grown structures changes from rods to flowers and flakes with doping. The microstructural analysis is carried out using HRTEM analysis. Various polar and non-polar optical modes present in the samples are analysed using Raman spectra. Two dominant emission bands around ~391 nm, ~470 nm are noticed from photoluminescence (PL) spectra. The potential of the Sn doped ZnO nanostructures in photocatalytic degradation of Rhodamine B is investigated and found that 1 mol% Sn doped ZnO exhibits superior photo catalytic performance.
( K. Sirisha ),( C. Ganesh Kumar ),( Kallaganti Venkata Siva Ramakrishna ),( Shravan Kumar Gunda ) 한국미생물생명공학회(구 한국산업미생물학회) 2017 한국미생물·생명공학회지 Vol.45 No.3
In the enduring investigation of the bioactive microbes, Pseudomonas aeruginosa strain (referred to as CGK-KS-1 (ICTB-315)), isolated from Chumathang hot spring, Ladakh, and India, was identified to possess a major bioactive fraction with antimicrobial and anti-biofilm properties. This bioactive metabolite was purified through bioactivity-guided fractionation. The chemical structure of this major compound was elucidated as phenazine-1-carboxamide (PCN) based on <sup>1</sup>H and <sup>13</sup>C NMR, FT-IR, EI-HR-MS and 2D NMR spectroscopic techniques. In the current study, PCN exhibited antimicrobial activity with MIC values ranging between 1.9-3.9 μg/ml against various test human pathogens and Xanthomonas spp. PCN showed the antibiofilm property with the IC<sub>50</sub> values ranging from 17.04 to 60.7 μM against different test pathogens. The in silico docking studies showed PCN strongly interacted with various proteins of different Xanthomonas spp. with high binding energies. We report herein for the first time the anti-biofilm property and the docking studies of PCN. The extrolite from P. aeruginosa strain CGK-KS-1 showed promising bioactivities and may be considered as a potential candidate for application in various biocontrol strategies.
( C. Ganesh Kumar ),( Poornima Mongolla ),( Cheemalamarri Chandrasekhar ),( Yedla Poornachandra ),( Bandi Siva ),( K. Suresh Babu ),( Kallaganti Venkata Siva Ramakrishna ) 한국미생물생명공학회(구 한국산업미생물학회) 2017 한국미생물·생명공학회지 Vol.45 No.3
Actinobacteria are prolific producers of a large number of natural products with diverse biological activities. In the present study, an actinobacterium isolated from sunflower rhizosphere soil sample collected from Medak, Andhra Pradesh, South India was identified as Amycolatopsis thermoflava strain SFMA-103. A pigmented secondary metabolite in culture broth was extracted by using methanol and it was further purified by silica gel column chromatography with methanol-chloroform solvent system. Structural elucidation studies based on UV-visible, 1D and 2D-NMR, FT-IR, and mass spectroscopic analyses confirmed the structure as 1-methoxy-3-methyl-8-hydroxy-anthraquinone. It showed significant in vitro anticancer activity against lung cancer and lymphoblastic leukemia cells with IC<sub>50</sub> values of 10.3 and 16.98 μM, respectively. In addition, 1-methoxy-3-methyl-8-hydroxy-anthraquinone showed good free radical scavenging activity by DPPH method with an EC<sub>50</sub> of 18.2 μg/ml. It also showed other promising superoxide radical scavenging, nitric oxide radical scavenging and inhibition of lipid peroxidation activities. This is a first report of antiproliferative and antioxidant activities of 1-methoxy-3-methyl-8-hydroxy-anthraquinone isolated from A. thermoflava strain SFMA-103 which may find potential application in biotechnological and pharmaceutical fields.
P. Ganesh Kumar,D. Sakthivadivel,K. Balaji,Mohammed Salman,Sung Chul Kim 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.6
A double-pass solar air heater (DPSAH) with shot blasting and winglets in the air passage is a recommended cost-effective design development to enhance thermal performance. Three different absorber plate configurations for DPSAHs were experimentally tested: (a) V-corrugation with shot blasting, (b) V-corrugation with shot blasting and a 4-3 winglet pattern, and (c) V-corrugation with shot blasting with a 3-2 winglet pattern. Furthermore, aluminum winglets were welded to the DPSAH absorber plate to increase the channel turbulence to enhance the heat transfer performance. The pressure drop and thermal performance of the DPSAHs with and without winglets were investigated using meteorological parameters such as ambient temperature, wind speed, solar irradiance, and interior temperature at regular time intervals. V-corrugation with 4-3 winglets has a maximum thermal efficiency and pressure drop of 49.5 % and 230 Pa, respectively, at a mass flow rate of 0.02 m/s. Results showed that the thermal efficiency of V-corrugation with 4-3 winglets was improved by a maximum of 7 % compared with the V-corrugation air heater. Finally, increasing the mass flow rate from 0.01 kg/s to 0.02 kg/s increases the pressure drop by 1.22 times for the V-corrugation and 1.3 times for the V-corrugation with 4-3 winglets. Furthermore, a complete economic study of DPSAHs for India is examined in this article.
Empirical Investigations to Find Illegal and its Equivalent Test Cases using RANDOM-DELPHI
J. Ratna Kumar,K. Koteswara Rao,D. Ganesh 보안공학연구지원센터 2015 International Journal of Software Engineering and Vol.9 No.11
Software quality can be described as degree of conformance to functional, performance requirements, implicit and explicit characteristics and specified document development standards. To assure software quality testing and debugging both have to be accommodate Testing is a sequence of steps that can be applied to achieve or uncover bugs, which can be rated as mild, moderate, serious, seriously disturbing, extreme, intolerable, and catastrophic. Sometimes low frequency bugs may not detected using normal testing, but causes huge disaster like Ariane 5, Patriot missile, Mars path finder. To uncover low frequency bugs Random Testing (RT) is a best alternative strategy. However random testing is useful technique for testing, the implications of random testing are not to choose random testing as their testing technique for the testers. In this paper we present the implications of random testing and to prove these implications with the FTP-Client Server application.
Enhancing Gender Classification in Social Networks
S. Nalini,K. Sandhya,P. Ganesh Kumar 한국산업정보학회 2014 한국산업정보학회 학술대회논문집 Vol.2014 No.1
Posts in social networking sites are informal, personal writings that people post on their own blog or sites. Nowadays, blogging in Social Networks are an important online activity. With the rapid growth of blogs in Social Network, their value as an important source of information is increasing. A large amount of research work has been devoted to blogs in the Natural Language Processing (NLP) and other communities. There are also many commercial companies that exploit information in blogs to provide value-added services, e.g. search, topic tracking, and sentiment analysis of people’s opinions on products and services. The goal of our system is to identify author gender of posts in social networks coming from a wide variety of source. The dataset used is from the popular social network, Twitter. Using the parts of speech of the dataset the gender of the site user is found. Empirical evaluation using a real-life blog data set shows that these two techniques improve the classification accuracy of the current state-of the-art methods significantly.
Design and optimization of concurrent tolerance in mechanical assemblies using bat algorithm
L. Ramesh Kumar,K. P. Padmanaban,S. Ganesh Kumar,C. Balamurugan 대한기계학회 2016 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.30 No.6
Concurrent designing of tolerance has become a vital concern in product and process development due to the relationship between quality, functionality and product cost. It is one of the well explored areas in combinatorial optimization. In this paper, a recently developed optimization algorithm, called Bat algorithm (BA), is used for optimizing the tolerance based on concurrent objectives to minimize the manufacturing cost, present worth of expected quality loss and quality loss. The mechanical assemblies such as Bevel gear assembly (A), Gear box assembly (B) and Suction union assembly (C) are considered to demonstrate the proposed algorithm. It is found that the BA has produced better results than other methods in initial generations for concurrent tolerance problems.
( Kamal ),( Ahmed ),( Anver Basha Shaik ),( C. Ganesh Kumar ),( Poornima Mongolla ),( P. Usha Rani ),( K. V. S ),( Rama Krishna ),( Suman Kumar Mamidyala ),( Joveeta Joseph ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.1
In an ongoing survey of the bioactive potential of microorganisms from Ladakh, India, the culture medium of a bacterial strain of a new Pseudomonas sp., strain ICTB-745, isolated from an alkaline soil sample collected from Leh, Ladakh, India, was found to contain metabolites that exhibited broad-spectrum antimicrobial and biosurfactant activities. Bioactivity-guided purification resulted in the isolation of four bioactive compounds. Their chemical structures were elucidated by 1H and 13C NMR, 2D-NMR (HMBC, HSQC, 1H,1H-COSY, and DEPT- 135), FT-IR, and mass spectroscopic methods, and were identified as 1-hydroxyphenazine, phenazine-1-carboxylic acid (PCA), rhamnolipid-1 (RL-1), and rhamnolipid-2 (RL-2). These metabolites exhibited various biological activities like antimicrobial and efficient cytotoxic potencies against different human tumor cell lines such as HeLa, HepG2, A549, and MDA MB 231. RL-1 and RL-2 exhibited a dose-dependent antifeedant activity against Spodoptera litura, producing about 82.06% and 73.66% antifeedant activity, whereas PCA showed a moderate antifeedant activity (63.67%) at 60 μg/cm2 area of castor leaf. Furthermore, PCA, RL-1, and RL-2 exhibited about 65%, 52%, and 47% mortality, respectively, against Rhyzopertha dominica at 20 μg/ml. This is the first report of rhamnolipids as antifeedant metabolites against Spodoptera litura and as insecticidal metabolites against Rhyzopertha dominica. The metabolites from Pseudomonas sp. strain ICTB-745 have interesting potential for use as a biopesticide in pest control programs.
Transcriptome Analysis of Early Responsive Genes in Rice during Magnaporthe oryzae Infection
Wang, Yiming,Kwon, Soon Jae,Wu, Jingni,Choi, Jaeyoung,Lee, Yong-Hwan,Agrawal, Ganesh Kumar,Tamogami, Shigeru,Rakwal, Randeep,Park, Sang-Ryeol,Kim, Beom-Gi,Jung, Ki-Hong,Kang, Kyu Young,Kim, Sang Gon,K The Korean Society of Plant Pathology 2014 Plant Pathology Journal Vol.30 No.4
Rice blast disease caused by Magnaporthe oryzae is one of the most serious diseases of cultivated rice (Oryza sativa L.) in most rice-growing regions of the world. In order to investigate early response genes in rice, we utilized the transcriptome analysis approach using a 300 K tilling microarray to rice leaves infected with compatible and incompatible M. oryzae strains. Prior to the microarray experiment, total RNA was validated by measuring the differential expression of rice defense-related marker genes (chitinase 2, barwin, PBZ1, and PR-10) by RT-PCR, and phytoalexins (sakuranetin and momilactone A) with HPLC. Microarray analysis revealed that 231 genes were up-regulated (>2 fold change, p < 0.05) in the incompatible interaction compared to the compatible one. Highly expressed genes were functionally characterized into metabolic processes and oxidation-reduction categories. The oxidative stress response was induced in both early and later infection stages. Biotic stress overview from MapMan analysis revealed that the phytohormone ethylene as well as signaling molecules jasmonic acid and salicylic acid is important for defense gene regulation. WRKY and Myb transcription factors were also involved in signal transduction processes. Additionally, receptor-like kinases were more likely associated with the defense response, and their expression patterns were validated by RT-PCR. Our results suggest that candidate genes, including receptor-like protein kinases, may play a key role in disease resistance against M. oryzae attack.