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Kim, Jungju,Park, Yongdoo,Tae, Giyoong,Lee, Kyu Back,Hwang, Chang Mo,Hwang, Soon Jung,Kim, In Sook,Noh, Insup,Sun, Kyung Wiley Subscription Services, Inc., A Wiley Company 2009 Journal of biomedical materials research. Part A Vol.a88 No.4
<P>Hyaluronic acid is a natural glycosaminoglycan involved in biological processes. Low-molecular-weight hyaluronic acid (10 and 50 kDa)-based hydrogel was synthesized using derivatized hyaluronic acid. Hyaluronic acid was acrylated by two steps: (1) introduction of an amine group using adipic acid dihydrazide, and (2) acrylation by N-acryloxysuccinimide. Injectable hyaluronic acid-based hydrogel was prepared by using acrylated hyaluronic acid and poly(ethylene glycol) tetra-thiols via Michael-type addition reaction. Mechanical properties of the hydrogel were evaluated by varying the molecular weight of acrylated hyaluronic acid (10 and 50 kDa) and the weight percent of hydrogel. Hydrogel based on 50-kDa hyaluronic acid showed the shortest gelation time and the highest complex modulus. Next, human mesenchymal stem cells were cultured in cell-adhesive RGD peptide-immobilized hydrogels together with bone morphogenic protein-2 (BMP-2). Cells cultured in the RGD/BMP-2-incorporated hydrogels showed proliferation rates higher than that of control or RGD-immobilized hydrogels. Real-time RT-PCR showed that the expression of osteoblast marker genes such as CBFα1 and alkaline phosphatase was increased in hyaluronic acid-based hydrogel, and the expression level was dependent on the molecular weight of hyaluronic acid, RGD peptide, and BMP-2. This study indicates that low-molecular-weight hyaluronic acid-based hydrogel can be applied to tissue regeneration as differentiation guidance materials of stem cells. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009</P>
Osteogenic Response of MC3T3-E1 and Raw264.7 in the 3D-Encapsulated Co-Culture Environment
Kim Jungju,Lyu Hao-Zhen,Jung Chisung,Lee Kyung Mee,Han Shi Huan,Lee Jae Hyup,Cha Misun 한국조직공학과 재생의학회 2021 조직공학과 재생의학 Vol.18 No.3
Background: Three-dimensional (3D) in vitro cultures recapitulate the physiological microenvironment and exhibit high concordance with in vivo conditions. Improving co-culture models with different kind of cell types cultured on a 3D scaffold can closely mimic the in vivo environment. In this study, we examined the osteogenic response of pre-osteoblast MC3T3-E1 cells and Raw264.7 mouse monocytes in a 3D-encapsulated co-culture environment composed of the Cellrix® 3D culture system, which provides a physiologically relevant environment. Methods: The Cellrix® 3D Bio-Gel scaffolds were used to individually culture or co-culture two type cells in 3D microenvironment. Under 3D culture conditions, osteoblastic behavior was evaluated with an ALP assay and staining. ACP assay and TRAP staining were used as osteoclastic behavior indicator. Results: Treatment with osteoblastic induction factors (+3F) and RANKL had on positively effect on alkaline phosphatase activity but significantly inhibited to acid phosphatase activity during osteoclastic differentiation in 3D co-culture. Interestingly, alkaline phosphatase activity or acid phosphatase activity in 3D co-culture was stimulated with opposite differentiation factors at an early stage of differentiation. We guess that these effects may be related to RANK–RANKL signaling, which is important in osteoblast regulation of osteoclasts. Conclusion: In this study, the osteogenic response of 3D encapsulated pre-osteoblast MC3T3-E1 cells and mouse monocyte Raw264.7 cells was successfully demonstrated. Our 3D culture conditions will be able to provide a foundation for developing a high-throughput in vitro bone model to study the effects of various drugs and other agents on molecular pathways. Background: Three-dimensional (3D) in vitro cultures recapitulate the physiological microenvironment and exhibit high concordance with in vivo conditions. Improving co-culture models with different kind of cell types cultured on a 3D scaffold can closely mimic the in vivo environment. In this study, we examined the osteogenic response of pre-osteoblast MC3T3-E1 cells and Raw264.7 mouse monocytes in a 3D-encapsulated co-culture environment composed of the Cellrix® 3D culture system, which provides a physiologically relevant environment. Methods: The Cellrix® 3D Bio-Gel scaffolds were used to individually culture or co-culture two type cells in 3D microenvironment. Under 3D culture conditions, osteoblastic behavior was evaluated with an ALP assay and staining. ACP assay and TRAP staining were used as osteoclastic behavior indicator. Results: Treatment with osteoblastic induction factors (+3F) and RANKL had on positively effect on alkaline phosphatase activity but significantly inhibited to acid phosphatase activity during osteoclastic differentiation in 3D co-culture. Interestingly, alkaline phosphatase activity or acid phosphatase activity in 3D co-culture was stimulated with opposite differentiation factors at an early stage of differentiation. We guess that these effects may be related to RANK–RANKL signaling, which is important in osteoblast regulation of osteoclasts. Conclusion: In this study, the osteogenic response of 3D encapsulated pre-osteoblast MC3T3-E1 cells and mouse monocyte Raw264.7 cells was successfully demonstrated. Our 3D culture conditions will be able to provide a foundation for developing a high-throughput in vitro bone model to study the effects of various drugs and other agents on molecular pathways.
Effectiveness of radiotherapy for head and neck skin cancers: a single-institution study
Kim, Jae Wang,Yun, Byung Min,Shin, Myoung Soo,Kang, Jae Kyoung,Kim, JungJu,Kim, Young Suk The Korean Society for Radiation Oncology 2019 Radiation Oncology Journal Vol.37 No.4
Purpose: External beam radiotherapy (EBRT) is a useful option to treat head and neck skin cancer patients who are not indicated for surgery. In this study, we evaluated the treatment outcomes of EBRT in an Asian population. Materials and Methods: The records from 19 head and neck skin cancer patients (10 with squamous cell carcinoma and 9 with basal cell carcinoma) who were treated with definitive or adjuvant EBRT from 2009 to 2017 were retrospectively reviewed. The radiotherapy doses administered ranged from 50 to 66 Gy (median, 55 Gy) with 2.0-2.75 Gy per daily fraction (median, 2.5 Gy). The T stage at presentation was as follows: Tis (1 patient), T1 (11 patients), T2 (6 patients), and T3 (1 patient). None had regional lymph node disease or distant metastasis at presentation. The local failure-free survival (LFFS) rates, toxicity, and cosmetic results were analyzed. Results: The median age was 75.5 years (range, 52.6 to 92.5 years). The median follow-up duration from the completion of radiotherapy was 44.9 months (range, 5.8 to 82.6 months). One local failure occurred in a patient with a 2.1-cm posterior neck squamous cell carcinoma at 32.5 months after radiotherapy (1/19, 5.3%). The 3-year LFFS rate was 91.7%. No patients died from skin cancer during follow-up, and no grade 3 complications occurred. The cosmetic outcomes were excellent for 16 (84.2%) and good for 3 (15.8%) of the 19 patients. Conclusion: EBRT offers good local control and cosmetic outcomes in patients with head and neck skin cancer, with no grade 3 complications.
Dynamic behavior of hybrid poly(acrylic acid) gel prepared by γ-ray irradiated imogolite
Ryu, Jungju,Kim, Hyosin,Kim, Jungin,Ko, Jaehyoung,Sohn, Daewon Elsevier 2017 Colloids and surfaces. A, Physicochemical and engi Vol.535 No.-
<P><B>Abstract</B></P> <P>The dynamic behavior of imogolite-poly(acrylic acid) nanocomposite hydrogels was investigated using dynamic light scattering. The hydrogel was prepared with aluminosilicate nanotubes, called imogolite, whose surface was activated by γ-ray irradiation to initiate gelation. Time-resolved light scattering was used to explore the gelation mechanism via diffusion of the imogolite during reaction, and ensemble-averaged light scattering was used to probe the dynamic behavior of the completed gels where imogolite particles participate in chemical cross-linking. The imogolite has a key role in gelation kinetics and gel construction. Gelation progressed rapidly at higher imogolite concentrations, which is evidence of the participation of imogolite during the initiation process. The resulting hydrogels exhibited characteristic lengths that are shorter at higher concentrations of imogolite rods, which can supply a large number of radicals. This study discusses the internal structure of nanocomposite gels based on the dynamic behavior of a hydrogel chemically crosslinked from inorganic particles, which have been used to improve the mechanical properties of polymer gels.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
박정주(JungJu Park),김주혜(JuHaye Kim),김명희(Myoung-Hee Kim) 한국정보과학회 2005 한국정보과학회 학술발표논문집 Vol.32 No.1
본 논문에서는 CAVETM-like 시스템에서 역운동학을 이용하여 사용자의 인체 움직임을 실시간으로 추정하기 위한 기법을 제안한다. 사용자를 둘러싼 스크린으로 투사되는 빛에 인하여 매순간 변화하는 배경을 포함하는 영상으로부터 사용자 영역을 추출하기 위하여 적외선 반사 영상을 이용하였다. 이를 이용하여 추출된 사용자 컬러 영상에 프로젝션 기반 가상환경의 특성을 고려한 컬러 모델을 적용하여 사용자의 얼굴과 손 영역을 추출하였다. 위치 추정 단계를 통하여 다음 프레임에서의 관심영역 위치를 미리 예측하고 추출된 사용자의 손과 얼굴 위치 정보를 말단장치로 이용하고, 관절모델과 운동학적 제약조건을 기반으로 역운동학적인 방법을 통해 사용자의 동작을 추정하였다. 제안 기법에서는 별도의 마커나 도구를 사용하지 않기 때문에 비침입적이며 사용자 움직임의 제약을 최소화할 수 있기 때문에 보다 인간 중심적인 인터랙션을 위한 기반 기술로 이용될 수 있다.
Effectiveness of radiotherapy for head and neck skin cancers: a single-institution study
Jae Wang Kim,Byung Min Yun,Myoung Soo Shin,Jae Kyoung Kang,JungJu Kim,Young Suk Kim 대한방사선종양학회 2019 Radiation Oncology Journal Vol.37 No.4
Purpose: External beam radiotherapy (EBRT) is a useful option to treat head and neck skin cancer patients who are not indicated for surgery. In this study, we evaluated the treatment outcomes of EBRT in an Asian population. Materials and Methods: The records from 19 head and neck skin cancer patients (10 with squamous cell carcinoma and 9 with basal cell carcinoma) who were treated with definitive or adjuvant EBRT from 2009 to 2017 were retrospectively reviewed. The radiotherapy doses administered ranged from 50 to 66 Gy (median, 55 Gy) with 2.0-2.75 Gy per daily fraction (median, 2.5 Gy). The T stage at presentation was as follows: Tis (1 patient), T1 (11 patients), T2 (6 patients), and T3 (1 patient). None had regional lymph node disease or distant metastasis at presentation. The local failure-free survival (LFFS) rates, toxicity, and cosmetic results were analyzed. Results: The median age was 75.5 years (range, 52.6 to 92.5 years). The median follow-up duration from the completion of radiotherapy was 44.9 months (range, 5.8 to 82.6 months). One local failure occurred in a patient with a 2.1-cm posterior neck squamous cell carcinoma at 32.5 months after radiotherapy (1/19, 5.3%). The 3-year LFFS rate was 91.7%. No patients died from skin cancer during follow-up, and no grade 3 complications occurred. The cosmetic outcomes were excellent for 16 (84.2%) and good for 3 (15.8%) of the 19 patients. Conclusion: EBRT offers good local control and cosmetic outcomes in patients with head and neck skin cancer, with no grade 3 complications.