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Jung, H.A.,Ali, Md.Y.,Jung, H.J.,Jeong, H.O.,Chung, H.Y.,Choi, J.S. Elsevier Sequoia 2016 Journal of Ethnopharmacology Vol.191 No.-
Ethnopharmacological relevance: Semen Cassiae has been traditionally used as an herbal remedy for liver, eye, and acute inflammatory diseases. Recent pharmacological reports have indicated that Cassiae semen has neuroprotective effects, attributable to its anti-inflammatory actions, in ischemic stroke and Alzheimer's disease (AD) models. Aim of the study: The basic goal of this study was to evaluate the anti-AD activities of C. obtusifolia and its major constituents. Previously, the extract of C. obtusifolia seeds, was reported to have memory enhancing properties and anti-AD activity to ameliorate amyloid β-induced synaptic dysfunction. However, the responsible components of C. obtusifolia seeds in an AD are currently still unknown. In this study, we investigated the inhibitory effects of C. obtusifolia and its constituents against acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and β-site amyloid precursor protein (APP) cleaving enzyme 1 (BACE1) enzyme activity. Materials and methods: In vitro cholinesterase enzyme assays by using AChE, BChE, and BACE1 were performed. We also scrutinized the potentials of Cassiae semen active component as BACE1 inhibitors via enzyme kinetics and molecular docking simulation. Results: In vitro enzyme assays demonstrated that C. obtusifolia and its major constituents have promising inhibitory potential against AChE, BChE, and BACE1. All Cassiae semen constituents exhibited potent inhibitory activities against AChE and BACE1 with IC<SUB>50</SUB> values of 6.29-109@?g/mL and 0.94-190@?g/mL, whereas alaternin, questin, and toralactone gentiobioside exhibited significant inhibitory activities against BChE with IC<SUB>50</SUB> values of 113.10-137.74@?g/mL. Kinetic study revealed that alaternin noncompetitively inhibited, whereas cassiaside and emodin showed mixed-type inhibition against BACE1. Furthermore, molecular docking simulation results demonstrated that hydroxyl group of alaternin and emodin tightly interacted with the active site residues of BACE1 and their relevant binding energies (-6.62 and -6.89kcal/mol), indicating a higher affinity and tighter binding capacity of these compounds for the active site of BACE1. Conclusion: The findings of the present study suggest the potential of C. obtusifolia and its major constituents for use in the development of therapeutic or preventive agents for AD, especially through inhibition of AChE, BChE and BACE1 activities.
Jung, H.A.,Jung, H.J.,Jeong, H.Y.,Kwon, H.J.,Ali, Md.Y.,Choi, J.S. Elsevier 2014 Fitoterapia Vol.92 No.-
The dramatic increase in obesity-related diseases emphasizes the need to elucidate the cellular and molecular mechanisms underlying fat metabolism. Inhibition of adipocyte differentiation has been suggested to be an important strategy for preventing or treating obesity. In our previous study, we characterized an Ecklonia stolonifera extract and non-polar fractions thereof, including dichloromethane and ethyl acetate fractions. We showed that these fractions inhibited adipocyte differentiation and lipid formation/accumulation in 3T3-L1 preadipocytes, as assessed by Oil Red O staining. As part of our ongoing search for anti-obesity agents derived from E. stolonifera, in this work, we characterized five known phlorotannins, including phloroglucinol, eckol, dieckol, dioxinodehydroeckol, and phlorofucofuroeckol A, all of which were isolated from the active ethyl acetate fraction of E. stolonifera. We determined the chemical structures of these phlorotannins through comparisons of published nuclear magnetic resonance (NMR) spectral data. Furthermore, we screened these phlorotannins for their abilities to inhibit adipogenesis over a range of concentrations (12.5-100μM). Of these five phlorotannins, phloroglucinol, eckol, and phlorofucofuroeckol A significantly concentration-dependently inhibited lipid accumulation in 3T3-L1 cells without affecting cell viability. In addition, the five isolated phlorotannins also significantly reduced the expression levels of several adipocyte marker genes, including proliferator activated receptor γ (PPARγ) and CCAAT/enhancer-binding protein α (C/EBPα), although they did so to different extents. These results suggest that the molecular weight of a phlorotannin is an important factor affecting its ability to inhibit adipocyte differentiation and modulate the expression levels of adipocyte marker genes.
Jung, H. A.,Roy, A.,Choi, J. S. Springer Japan KK 2017 Fisheries Science Vol. No.
<P>Fucoxanthin is the primary carotenoid found in edible seaweeds, such as Eisenia bicyclis, Undaria pinnatifida, and others. The aim of this study was to determine the effectiveness of fucoxanthin against Parkinson's disease (PD) by inhibiting monoamine oxidase (MAO) A and B because MAO inhibitors are used in early management of PD. A sensitive enzyme-based chemiluminescent assay and kinetics methods were used to investigate the enzyme inhibitory activities and mode of inhibition. A molecular docking simulation was performed to clarify the binding characteristics of fucoxanthin to hMAO-A (2z5x) and hMAO-B (2v5z). Our results suggest that fucoxanthin shows significant inhibitory activity against hMAO-A and hMAO-B with IC50 values of 197.41 +/- 2.20 and 211.12 +/- 1.17 mu M, respectively. Selegiline was used as the positive control (IC50: 10.54 +/- 1.25 and 0.128 +/- 0.01 mu M for hMAO-A and hMAO-B, respectively). The enzyme-based kinetics results demonstrated that fucoxanthin inhibited both hMAOs in a reversible competitive manner. The molecular docking simulation predicted that fucoxanthin exhibits higher binding affinity towards hMAO-A and hMAO-B through hydrogen bonding and hydrophobic interactions. These findings suggest that fucoxanthin is a reversible competitive hMAO inhibitor that could be used to manage PD.</P>
Efficient Data Replication Scheme based on Hadoop Distributed File System
Jungha Lee,Jaehwa Chung,Daewon Lee 보안공학연구지원센터 2015 International Journal of Software Engineering and Vol.9 No.12
Hadoop distributed file system (HDFS) is designed to store huge data set reliably, has been widely used for processing massive-scale data in parallel. In HDFS, the data locality problem is one of critical problem that causes the performance decrement of a file system. To solve the data locality problem, we propose an efficient data replication scheme based on access count prediction in a Hadoop framework. By the previous data access count, the existing data replication scheme predicts the next access count of data files using Lagrange’s interpolation. Then, the proposed data replication scheme determines the replication factor with the predicted data access count, whether it generates a new replica or it uses the loaded data as cache selectively. Finally, the proposed scheme provides improvement of data locality. By performance evaluation, proposed efficient data replication scheme is compared with default data replication setting of Hadoop that shows proposed scheme reduces averagely 8.9% of the task completion time in the map phase. Regarding the data locality, proposed scheme provides the increase of node locality by 6.6% and the decrease of rack and rack-off locality by 38.9% and 56.5%.
Lacquer Techniques in the Late Joseon Dynasty
( Junghae Park ),( Jaewan Choi ),( Uicheon Lee ),( Minji Kang ),( Soochul Kim ) 한국목재공학회 2023 목재공학 Vol.51 No.2
This study conducted scientific analyses on eight objects of wooden lacquerware to understand the manufacturing techniques of wooden lacquerware in the late Joseon Dynasty. The results of lacquer layer analysis with a microscope and scanning electron microscopy-energy dispersive X-ray spectrometry revealed that most samples were composed of 1-3 lacquer layers. Moreover, a red-colored layer was found to be red ocher and cinnabar, and Fourier-transform infrared spectroscopy analysis was carried out to determine the components of the lacquer layer. The detected components were mostly lacquer and partially cashew shell nut liquid.