http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
유기용매 추출법에 의한 포플라의 전처리 및 당화 조건의 최적화
박정극,전영삼 東國大學校 1990 論文集 Vol.29 No.-
The effect of the pretreatment by solvent extraction with various solvent on the saccharification of poplar (Populus euramericana)was studied. The solvent system was phenol/H_2O (uncatalyzed, HCl and NaOH catalyzed), butanol/H_2O, ethanol/H_2O and ethylendiamine/H_2O solvent system. When the poplar was pretreated by phenol/H_2O uncatalyzed system, the best result of the enzymatic saccharification was total of 43.87 g/L reducing sugar produced and 83.35% of carbohydrated conversion was obatined at 190℃, 60 minutes. Total wood yield and the lignin removal were 46.3% and 96.17%, respectively. The use of acid, base catalyst and the others solvents was unsuitable to increase the efficiency of saccharification.
빙축열 냉방시스템과 흡수식 냉온수기의 경제성 비교 : 생인공전피의 냉도보존 방법의 개발에 관한 연구
박정극,박재섭 동국대학교 산업기술연구원 2001 산업기술논문집 Vol.12 No.2
본 연구에서는 조직공학적으로 만든 생인공진피를 그들의 기능적 특성을 유지하면서 장기간 냉동보존할 수 있는 방법을 개발하고자 하였는데, 이를 위해 내동보존시 세포에 가장 중요한 영향을 주는 인자들을 지표로하여 살펴보았고 thiazolyl blue (MTT) 분석을 통하여 세포의 회복률을 평가하였다. 또한 냉동속도 정확하게 원하는 속도로 맞추기 위해서 냉동프로그램을 개발하여 조직의 냉동에 사용하였다. 결과적으로 생인공진피는 냉동보호용액의 노출온도가 낮을수록 그리고 냉각속도가 느릴수록 보다 높은 세포 생존율을 나타내었으며, -196℃의 저장온도에서는 조직이 형태학적으로 변형을 일으킨 반면 -130℃ 이상의 저장온도에서는 조직이 육안적으로 integrity를 완전하게 유지하였다는 사실을 관찰할 수 있었다. In this study, out efforts were made to develope the long-term cryopreservation method of tissue-engineered bioartificial dermal equivalent keeping his own biological function. For this purpose, we studied the primary factors exerting on cell recovery at cryopreservation and thiazolyl blue (MTT) assay was carried out to measure cell recovery. Also new freezing program used for cooling bioartificial dermal equivalent was set up to lower a temperature to -90℃ at cooling rate of 1℃/min. As a conclusion, it was found that cell recovery tended to increase as the exposure temperature of cryoprtectant solution was down, and the cooling rate became lower. It was also shown that the samples cryopreserved at -196℃ didn't retain their integrity but ones cryopreserved above -130℃ retained it. So, this fact implies that storage above -130℃ is the safe way fitted in cryopreserving bioartificial dermal equivalent.
유기용매 추출법과 산가수분해에 의한 포플라의 전처리 및 효소당화액을 이용한 생물고분자의 생산에 관한 연구
김균성,박정극 동국대학교 산업기술대학원 1993 산업기술논문집 Vol.1 No.-
In this study the lignocellulosic biomass, Italy poplar, was pretreated by the chemical pretreatment method, i.e., organic solvent (Phenol/H_2O) extraction. Using Phenol/H_2O solvent system. Wood yield, % delignification and % sacchari-fication(72hours) of poplar after pretreatment at 190℃, 60 min. which was found out as the optimum pretreatment condition, were 46,3%, 96.1% and 83.35% (43.87 g/L) respectively. The possibility of reusing the spent organic solvent and recovery of hemicellulose fraction were also studied. By recycling spent black organic solvent in this process, we obtained an approximately identical results. The percent saccharification of 79.9% (43.6 g/L) was obtained from the recycled process and the degree of delignification was more than 99%. The delignification reactions using Phenol/H_2O solvent system showed two distinct mechanism of pseudo first order reactions (fast and slow), and the activation energy of the fast reaction was 38.4Kcal/gmol. For the recovery of hemicellulose fraction, poplar wood was pretreated using acid as a catalyst at high temperature and low concentration;180℃,0.05%∼0.15 vol% H_2SO_4(HTLC), and also at low temperature and high concentration;120℃,1∼4 vol% H_2SO_4(LTHC). 58% of hemicellulose was recovered whin the Italy poplar was pretreated at 180℃ with 0.1 vol% H_2SO_4 for 10 minutes (HTLC), and 74.4% recovery of hemicellulose fraction was obtained when it was pretreated at 120℃ with 4 vol% H_2SO_4 (LTHC) for 20 minutes, which is considered as an optimum condition for hemicellulose recovery. And after these acid hydrolysis followed by solvent extraction at 190℃, 60 min, the lower amount of sugar was obtained compared to one-step solvent extraction which was studied. As an application of saccharified broth, the culture of Zoogloea remigera 115 which produces biopolymer adsorbing heavy metals (Cu, Fe, Zn, Co etc) was conducted. When saccharified broth was used as a carbon source, the lag phase of the fermantation was longer than that of synthetic glucose media. After 60 hours of fermentation, the maximum cell density of 1.5 g/L was obtained. Final biopolymer concentration was 10.05 g/L after 200 hours of fermentation. Above experimental results showed the possibility of using saccharified broth as a carbon sourse for fermentation media.
이두훈,윤희훈,박정극 한국화학공학회 2004 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.42 No.2
우리 인체의 장기 중 간(liver)은 생존에 그 어느 장기보다도 중요한 역할을 담당하는 장기로서 여러 가지 원인으로 인하여 갑자기 간의 기능이 상실된 환자에게는 간이식만이 유일한 대안이다. 간세포를 이용한 체외 순환형 간 보조 장치(생인공간, bioartificial liver)는 최근 유일하게 전격성 간부적 환자의 생존을 중대 효과가 검증되었고 간이식이나 환자 자신의 간 재생으로의 생명 연장 교량 역할을 효과적으로 수행할 수 있을 것으로 기대되고 있다. 본 논문에서는 생인공간의 개념과 임상시험 중인 대표적인 생인공간 시스템 현황에 대해서 공학적 관점 위주로 소개하고 향후 이 분야에 대한 연구전망에 대하여 서술하였다. The liver is mutifunctional vital organ for healthy survival and orthotopic liver transplantation is the only definitive therapy for patients with acute or fulminant hepatic failure(FHF) so far. Bioartificial liver, a hepatocyte-based extracorporeal liver support system, enhanced the survival rates of patients with FHF and expected to act as a bridge to provide the extension of survival time until a donor organ becomes available for transplantation or their own liver can be regenerated. In this paper, we introduce concepts of bioartificial liver and review representative bioartifical liver systems in clinical trials with engineering issue. Finally we describe future perspectives of bioartificial liver.