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      • 유기용매 추출법과 산가수분해에 의한 포플라의 전처리 및 효소당화액을 이용한 생물고분자의 생산에 관한 연구

        김균성,박정극 동국대학교 산업기술대학원 1993 산업기술논문집 Vol.1 No.-

        In this study the lignocellulosic biomass, Italy poplar, was pretreated by the chemical pretreatment method, i.e., organic solvent (Phenol/H_2O) extraction. Using Phenol/H_2O solvent system. Wood yield, % delignification and % sacchari-fication(72hours) of poplar after pretreatment at 190℃, 60 min. which was found out as the optimum pretreatment condition, were 46,3%, 96.1% and 83.35% (43.87 g/L) respectively. The possibility of reusing the spent organic solvent and recovery of hemicellulose fraction were also studied. By recycling spent black organic solvent in this process, we obtained an approximately identical results. The percent saccharification of 79.9% (43.6 g/L) was obtained from the recycled process and the degree of delignification was more than 99%. The delignification reactions using Phenol/H_2O solvent system showed two distinct mechanism of pseudo first order reactions (fast and slow), and the activation energy of the fast reaction was 38.4Kcal/gmol. For the recovery of hemicellulose fraction, poplar wood was pretreated using acid as a catalyst at high temperature and low concentration;180℃,0.05%∼0.15 vol% H_2SO_4(HTLC), and also at low temperature and high concentration;120℃,1∼4 vol% H_2SO_4(LTHC). 58% of hemicellulose was recovered whin the Italy poplar was pretreated at 180℃ with 0.1 vol% H_2SO_4 for 10 minutes (HTLC), and 74.4% recovery of hemicellulose fraction was obtained when it was pretreated at 120℃ with 4 vol% H_2SO_4 (LTHC) for 20 minutes, which is considered as an optimum condition for hemicellulose recovery. And after these acid hydrolysis followed by solvent extraction at 190℃, 60 min, the lower amount of sugar was obtained compared to one-step solvent extraction which was studied. As an application of saccharified broth, the culture of Zoogloea remigera 115 which produces biopolymer adsorbing heavy metals (Cu, Fe, Zn, Co etc) was conducted. When saccharified broth was used as a carbon source, the lag phase of the fermantation was longer than that of synthetic glucose media. After 60 hours of fermentation, the maximum cell density of 1.5 g/L was obtained. Final biopolymer concentration was 10.05 g/L after 200 hours of fermentation. Above experimental results showed the possibility of using saccharified broth as a carbon sourse for fermentation media.

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