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RISS 인기검색어
- 검색키워드
- 저자 : Junbao Wang (검색결과 4 건)
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A New Calculation Method for Tunneling-Caused Stratum Settlement
Junbao Wang,Pengyuan Zhou,Zhanping Song,Shihao Li,Qiang Zhang 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.6
To improve the prediction accuracy for the stratum settlement induced by tunnels and obtain a theoretical method for determining the influence radius of settlement, a new empirical formula for the surface settlement curve is proposed in this study, and the slice method is introduced into the calculation of the influence radius of settlement. It is assumed that the disturbance boundary of the tunnel is a circular arc surface, the disturbed soil is divided into multiple slices, and the sliding force and resistance on the circular arc are obtained through a stress analysis of the slices. An arc with the same values of the sliding force and resistance can be determined as the actual disturbance boundary of the overlying strata as formed by the subsurface tunneling. On this basis, the influence radius of settlement and maximum settlement at different depths can be determined, and the settlement curve can be depicted by substituting the influence radius and maximum settlement into the expression of the settlement curve. The rationality of the proposed method is verified based on four sets of measured data. The surface settlement curves and the settlements at different depths on the center line of the tunnel obtained by the new method are generally consistent with the measured data.
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Transcription factor Sp1 is necessary and functional in regulating expression of oncogene ZNF703
Xiaolin Liao,Yongjie Lu,Junbao Yang,Tao Kuang,Lilin Jiang,Yanjun Wang,Huiqun Kang,Bo Jiang,Xiaoli Zhou,Sheng He 한국유전학회 2017 Genes & Genomics Vol.39 No.10
Zinc finger protein 703 (ZNF703) is a putative oncogene in patients with the luminal B molecular subtype of breast cancer. Although the exact function of ZNF703 protein remains largely unknown, its expression and regulation have been implicated in several physiological and pathological processes. In the current study, for the first time, we identified and characterized the human ZNF703 gene promoter region. As a means of characterizing the transcription elements required for expression of ZNF703 protein at different stages, we cloned the promoter region of ZNF703 then created chimeric reporter plasmids for use in luciferase assays. A progressive deletion analysis of the ZNF703 gene’s 5′ and 3′ -flanking regions revealed that the core promoter is located in a 256-bp region ranging from nt-539 to nt-283. Next, we examined the effects of sitespecific mutations and treatment with mithramycin A to identify the functional Sp1 binding site, which was found to be located in a 447 bp region that ranged from nt-509 to nt-76, displayed the characteristics of a CpG island, and overlapped with the promoter region. In conclusion, our data suggest that ZNF703 transcription is regulated by transcription factor Sp1. This finding should facilitate future studies of the mechanism which regulates expression of this important gene.
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Zhanping Song,Qiang Zhang,Yuwei Zhang,Junbao Wang,Shengyuan Fan,Guannan Zhou 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.11
To study the abnormal precursory information of rock before failure, uniaxial compression tests were carried out on sandstone specimens; meanwhile, the infrared thermal camera was utilized to monitor the infrared radiation temperature (IRT) variation of the specimen surface synchronously during failure process. The test results show that with the increases of strain, the average infrared radiation temperature (AIRT) decreased gradually; when strain raises to a certain degree, the AIRT increased abruptly; thereafter, the AIRT decreased gradually with increasing strain. Therefore, the mutation point of AIRT can be regarded as the abnormal precursory information of sandstone before failure. Through analysis, the average stress at the abnormal point was 79.93% of the uniaxial compression strength of the sandstone specimens tested in this paper, and the average strain at this point was 0.9%. In addition, due to the end effect, the IRT field distribution of the sandstone specimen surface presented a decreasing tendency from top to bottom at the initial loading stage. As strain increases, the IRT field distribution tended to be high in middle and low in two ends. Closing to failure, a Y-shaped high-temperature band caused by the shear failure of the specimen appeared in the infrared thermal image. After comparison, the position of the Y-sharped high-temperature band is basically corresponding to the actually failure position of the specimen. With the increases of strain, the IRT distribution ranges decreased and the IRT frequency distribution histogram form gradually approached to the normal distribution.
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Physiological responses of yellow‑horn seedlings to high temperatures under drought condition
Hua Jin,Jixiang Zou,Linlin Li,Xinlei Bai,Tong Zhu,Junbao Li,Bing-Cheng Xu,Zhi Wang 한국식물생명공학회 2020 Plant biotechnology reports Vol.14 No.1
Drought and hot stresses are the primary limiting factors for the growth and seed production of yellow-horn (Xanthoceras sorbifolia Bunge), especially in Xinjiang Province of China. A better understanding of its physiological and biochemical characteristics under drought–hot stress is requisite for its efficient cultivation in the arid and semi-arid areas. In this study, the physiological and biochemical responses of 3-month-old yellow-horn seedlings were evaluated after 7 days of treatments of five temperatures (25 °C/20 °C, 30 °C/25 °C, 35 °C/25 °C, 40 °C/25 °C and 45 °C/25 °C) under two watering conditions [adequate water supply (HW): 80 ± 5% FC (field capacity); low water supply (LW): 40 ± 5% FC] by a pot experiment. The increased temperature (above to 35 °C) markedly aggravated the damage on biomass accumulation and membrane integrity of yellow-horn seedlings under the LW. The accumulation of soluble sugars and proteins exhibited a growing trend as the temperature increased from 25 to 40 °C, which declined when the temperature was higher than 40 °C except for the soluble protein accumulation under HW. In addition, captured responsive characteristics of the gas-exchange parameters and chlorophyll fluorescence have indicated that the combinations of high temperature (above to 35 °C) and LW induced significant decrease in photosynthetic activities of yellow-horn seedlings. All these results showed that the hot stress significantly aggravated the drought damage on yellow-horn plant growth, especially when the temperature increased above 35 °C under drought stress.
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