Brand image design of homing tribal tourist attractions

Shen Yi,Wu Jing 한국디자인학회 2023 한국디자인학회 국제초대전도록 Vol.2023 No.10

Incorporation of Nasutitermes takasagoensis Endoglucanase into Cell Surface-Displayed Minicellulosomes in Pichia pastoris X33

( Jing Shen Ou ),( Yi Cheng Cao ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.9

In this study, the yeast Pichia pastoris was genetically modified to assemble minicellulosomes on its cell surface by the heterologous expression of a truncated scaffoldin CipA from Clostridium acetobutylicum. Fluorescence microscopy and western blot analysis confirmed that CipA was targeted to the yeast cell surface and that NtEGD, the Nasutitermes takasagoensis endoglucanase that was fused with dockerin, interacted with CipA on the yeast cell surface, suggesting that the cohesin and dockerin domains and cellulose-binding module of C. acetobutylicum were functional in the yeasts. The enzymatic activities of the cellulases in the minicellulosomes that were displayed on the yeast cell surfaces increased dramatically following interaction with the cohesin-dockerin domains. Additionally, the hydrolysis efficiencies of NtEGD for carboxymethyl cellulose, microcrystal cellulose, and filter paper increased up to 1.4-fold, 2.0-fold, and 3.2-fold, respectively. To the best of our knowledge, this is the first report describing the expression of C. acetobutylicum minicellulosomes in yeast and the incorporation of animal cellulases into cellulosomes. This strategy of heterologous cellulase incorporation lends novel insight into the process of cellulosome assembly. Potentially, the surface display of cellulosomes, such as that reported in this study, may be utilized in the engineering of S. cerevisiae for ethanol production from cellulose and additional future applications.

Spatial analysis of tuberculosis treatment outcomes in Shanghai: implications for tuberculosis control

Zhang Jing,Shen Xin,Yang Chongguang,Chen Yue,Guo Juntao,Wang Decheng,Zhang Jun,Lynn Henry,Hu Yi,Pan Qichao,Zhang Zhijie 한국역학회 2022 Epidemiology and Health Vol.44 No.-

OBJECTIVES: Tuberculosis (TB) treatment outcomes are a key indicator in the assessment of TB control programs. We aimed to identify spatial factors associated with TB treatment outcomes, and to provide additional insights into TB control from a geographical perspective.METHODS: We collected data from the electronic TB surveillance system in Shanghai, China and included pulmonary TB patients registered from January 1, 2009 to December 31, 2016. We examined the associations of physical accessibility to hospitals, an autoregression term, and random hospital effects with treatment outcomes in logistic regression models after adjusting for demographic, clinical, and treatment factors.RESULTS: Of the 53,475 pulmonary TB patients, 49,002 (91.6%) had successful treatment outcomes. The success rate increased from 89.3% in 2009 to 94.4% in 2016. The successful treatment outcome rate varied among hospitals from 78.6% to 97.8%, and there were 12 spatial clusters of poor treatment outcomes during the 8-year study period. The best-fit model incorporated spatial factors. Both the random hospital effects and autoregression terms had significant impacts on TB treatment outcomes, ranking 6th and 10th, respectively, in terms of statistical importance among 14 factors. The number of bus stations around the home was the least important variable in the model.CONCLUSIONS: Spatial autocorrelation and hospital effects were associated with TB treatment outcomes in Shanghai. In highly-integrated cities like Shanghai, physical accessibility was not related to treatment outcomes. Governments need to pay more attention to the mobility of patients and different success rates of treatment among hospitals.

Unambiguous Bearing Estimation of Coherent Signals Using Acoustic Vector Sensor

Yan Jing,Yi Shen,Naizhang Feng,Mingjian Sun 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.7

중국Young Consumer 라이프스타일과 IT소비

진징 ( Jing Jin ),센이 ( Yi Shen ),이재학 ( Jaehak Lee ),박철 ( Cheol Park ) 고려대학교 세종경영연구소 2017 경상논집 Vol.37 No.1

본 연구는 중국 Young Consumer의 쇼핑라이프스타일과 IT소비 간의 관계를 밝히고자 하였다. 이를 위해 중국 베이징, 상하이, 길림성의 20, 30대 소비자 227명을 대상으로 실증 분석하였다. 요인분석을 통해 중국 Young Consumer들의 라이프스타일을 편리적구매형, 경제적구매형, 충동적구매형, 개성적구매형, 혁신적구매형 등 5가지 차원으로 분류하였다. 또, 중국 Young Consumer들의 5가지 라이프스타일과 IT소비 간의 차이를 살펴보기 위하여 군집분석 후 분산분석을 실시하였다. 5가지 라이프스타일 중 편리적 구매형 즉 가기 쉽고 교통이 편리한 점포를 선호하며 구매 시 오래서서 기다림을을 싫어하는 특징들이 동일하게 나타났다. 중국 Young Consumer 들은 기존과 다른 새로운 소비집단으로써 다른 세대들과 쇼핑성향이 달랐다. 특히, IT소비에서 본 연구에서 살펴본 바와 같이 중국 Young Consumer들은 휴대폰 교체주기가 빠르고 제품의 기능사용이 아닌 개성을 표현하는 방식으로 생각 하고 있었다. 본 연구는 IT소비에 대한 중국 Young Consumer 들의 전반 행동과 구매에 대하여 영향을 미치는 요인들을 분석함으로써 중국에 진출할 기업들에게 기초 자료를 제공하고 중국 Young Consumer특성에 맞는 마케팅 방안을 제공할 수 있을 것이다. This study examined the relationship between shopping lifestyle of young chinese consumer and their consumption related to IT. A questionnaire survey of 227 Chineses young consumers was performed to cluster lifestyle group. As the results, there found 5 groups among young chinese consumer; convenient, economic, impulsive, unique, and innovative shopping styles. Threre were significant differences in demographics, internet shopping behaviors, and usage behaviors of mobile phone among 5 groups. The implications and further research directions were suggested in conclusion

Dynamics and Liver Disease Specific Aspects of Quality of Life Among Patients with Chronic Liver Disease in Yunnan, China

Che, Yan-Hua,You, Jing,Chongsuvivatwong, Virasakdi,Li, Li,Sriplung, Hucha,Yan, Yuan-Zhi,Ma, Si-Jia,Zhang, Xiaoli,Shen, Ting,Chen, He-Min,Rao, Shao-Feng,Zhang, Ru-Yi Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.12

Background: Patients with chronic liver diseases (CLD) may have compromised health related quality of life (HRQoL). Hepatitis B virus (HBV) infection has long been the leading cause of CLD including liver cancer and cirrhosis. Knowledge on different symptom profiles of CLD should help in development of comprehensive treatment and patient care plans. Objective: To access the facets of HRQoL in chronic liver diseases throughout their spectrum of severity. Materials and Methods: A cross-sectional study was conducted in the First Affiliated Hospital of Kunming Medical University in Yunnan Province of China. Both out- and inpatients undergoing treatment protocols for different HBV related liver disease states were consecutively collected from December 2012 to June 2013. ANOVA was used to compare the mean scores of EQ-5D and chronic liver disease questionnaire (CLDQ) among 5 disease groups. The relationship between demographic variables predicting global CLDQ scores and the domains of CLDQ was analysed. Results: A total of 1040 patients including 520 without complications, 91 with compensated cirrhosis, 198 with decompensated cirrhosis, 131 with HCC and 100 with liver failure were recruited. All domains of CLDQ, the means of EQ-5D value and EQ VAS exhibited significant decline with worsening of disease severity from uncomplicated HBV to liver failure. The multivariate regression demonstrated the reduction of mean scores of CLDQ domain at advanced stage. Patients with liver failure and HCC had more HRQoL impairment than other disease states. No effect of patient gender was found. Patient age was associated with 'fatigue' and 'worry' domains (p=0.006; p=0.004) but not with other domains and global scores of CLDQ and ED-5D. Conclusions: The HRQoL in chronic hepatitis B patients is greatly affected by disease states. Care for HBV-related diseases should consider not only the outcomes of treatment strategies but also improvement in patient wellbeing.

Historical Long-term Exposure to Pentachlorophenol Causing Risk of Cancer - A Community Study

Zheng, Rui-Zhi,Zhang, Qing-He,He, Yi-Xin,Zhang, Qian,Yang, Lin-Shen,Zhang, Zhi-Hua,Zhang, Xiu-Jun,Hu, Jing-Ting,Huang, Fen Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.2

Background: Pervious studies suggested occupational workers exposure to pentachlorophenol (PCP) might contribute to increased risk of cancer. However, few studies have focused on associations between PCP and cancer risk at the community level. Objective: The present study was to explore the cancer risk for the community population living long-term in a PCP contaminated area. Methods: All the cancer cases diagnosed in 2009-2011 in Tongling City were collected. The cancer patients' residencies were geo-referenced in each district. The historical PCP usage for each district of Tongling was calculated as the PCP pollution index, which was further used to divide into PCP exposure categories. Standardized rate ratios (SRRs) of cancer incidence were applied to detect the cancer risk as exposure grade elevated. Correlation analysis was performed to analyze the relationship between PCP pollution and cancer incidence. Results: A total of 5,288 cancer cases (3,451 male and 1,837 female) were identified. PCP usage was correlated with the incidence of leukemia (r=0.88, P=0.002) for males, and with cancer of the esophagus for males (r=0.83, P=0.008) and females (r=0.71, P=0.020). Compared with the low exposure category, significant SRRs for total cancer sites was obtained for high PCP exposure category (SRR=1.61, 95%CI=1.59-1.62). Most SRR values of the cancer sites were significantly increased as exposure grade elevated and exposure time extended. Conclusion: The present study found that community residents living in the PCP contaminated area had increased risk of cancers. Leukemias, lymphomas and nasopharyngeal and esophageal cancers are most possibly associated with PCP exposure.

Associations of TNF-RII rs1061622 With Post-Traumatic Stress Disorder and Their Interplays on Serum Lipids Levels in Adolescents

Ji Cheng Zhang,Jin Hua Wang,Jun Yi Liu,Qi Wei Guo,Jia Lin,Yi Lin Shen,Ke Xin Jia,Jia Jing Cai,Guo Ming Su,Ding Zhi Fang 대한신경정신의학회 2023 PSYCHIATRY INVESTIGATION Vol.20 No.11

Objective To verify effects of rs1061622 at tumor necrosis factor-α receptor II (TNF-RII) gene (<i>TNF-RII</i>) on post-traumatic stress disorder (PTSD) and its interactive effects with PTSD on serum lipids levels in adolescents.Methods PTSD was measured by PTSD Checklist-Civilian Version (PCL-C) in 699 adolescent survivors at 6 months after Wenchuan earthquake in China. A polymerase chain reaction and restriction fragment length polymorphism assay were utilized for <i>TNF-RII</i> rs1061622 genotyping followed by verification using DNA sequencing. Serum triglycerides (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol were tested using routine methods.Results G (deoxyguanine) allele carriers had higher PCL-C scores than TT (deoxythymidine) homozygotes in female subjects. Female adolescents had higher PCL-C scores than male subjects in TT homozygotes. Predictors of PTSD prevalence and severity were different between G allele carriers and TT homozygotes. Subjects with PTSD had lower TG, TG/HDL-C, TC/HDL-C, and higher HDL-C than adolescents without PTSD in male G allele carriers. G allele carriers had higher TG/HDL-C and TC/HDL-C than TT homozygotes in male adolescents without PTSD, and lower TG and TG/HDL-C in male PTSD patients. G allele carriers had higher TG than TT homozygotes only in female adolescents without PTSD.Conclusion These results suggest reciprocal actions of <i>TNF-RII</i> rs1061622 with other factors on PTSD severity, interplays of <i>TNF-RII</i> rs1061622 with PTSD on serum lipid levels, and novel treatment strategies for PTSD and comorbidities of PTSD with hyperlipidemia among adolescents with different genetic backgrounds of <i>TNF-RII</i> rs1061622 after experiencing traumatic events.

Development of human IgE biosensor using Sezawa-mode SAW devices

Ying-Chung Chen,Wei-Tsai Chang,Chien-Chuan Cheng,Jing-Yi Shen,Kuo-Sheng Kao 한국물리학회 2014 Current Applied Physics Vol.14 No.4

This paper reports Sezawa-mode surface acoustic wave (SAW) devices with via-isolated cavity to construct the allergy biosensor. To fabricate Sezawa-mode SAW devices, the RF magnetron sputtering method for the growth of piezoelectric ZnO thin films are adopted and influences of the sputtering parameters are investigated. The optimal substrate temperature of 300 C, RF power of 120 W and sputtering pressure of 2 Pa were used to deposit piezoelectric ZnO films with a smooth surface, uniform grain size and strongly c-axis-orientated crystallization. A back-etched SAW resonator is used in this study. The wet etching of (100)-oriented silicon wafers is used to form a back-side cavity which is critical to the formation of a hopper cavity for holding bio-analytes. The remaining membrane structure silicon thickness was 25 mm. In this report, the chrome (Cr, 12 nm)/gold (Au, 66 nm) layer was initially deposited onto the sensing area of SAW devices as the binding layer for biochemical sensor. The resonance frequency of the Sezawa-mode SAW device is 1.497 GHz. The maximum sensitivity of the Sezawa-mode is calculated to be 4.44 106 cm2/g for human immunoglobulin-E (IgE) detection. The stability for human IgE detection is calculated to be 80% and the variation of the stability 3% was obtained after several tests.

디지털네이티브(Digital Native)척도 개발 및 검증

홍민지 ( Minji Hong ),우선연 ( Seonyeon Woo ),진징 ( Jin Jing ),센이 ( Shen Yi ),이재학 ( Jae Hak Lee ),박철 ( Cheol Park ) 고려대학교 세종경영연구소 2018 경상논집 Vol.38 No.1

본 연구는 Don Tapscott(2008)의 디지털네이티브(Digital Native)의 8가지 특성인 자유, 맞춤제작, 철저한 조사, 성실성, 협업, 엔터테인먼트, 속도, 혁신에 대한 척도를 개발하여, 한국과 중국의 젊은 세대들을 대상으로 검증해 보았다. 설문은 한국, 중국의 10대~30대, 남녀 506명이 응답하였으며, 수집된 설문지를 통해 디지털네이티브의 8가지 차원을 확인하였으며, 측정의 신뢰도와 타당성을 검증하였다. 또한 한국과 중국의 디지털네이티브 성향 차이를 살펴보고 IT소비 상관관계에 대해 알아보았다. 그 결과 디지털네이티브(Digital Native)의 8가지 요인은 IT 소비와 유의한 상관관계를 나타내었으며, 특히 엔터테인먼트, 자유, 혁신 속성에서 비교적 높은 상관관계가 있는 것으로 나타났다. 또한 디지털네이티브 성향이 높은 집단과 낮은 집단 간에는 IT 소비 면에서 거의 모두 유의한 차이가 나타났다. 본 연구결과는 한국과 중국의 디지털네이티브(Digital Native) 세대의 새로운 소비 특징과 소통 방법을 연구하여 그들에게 맞는 새로운 마케팅 방향을 제시할 수 있을 것이다. This study has developed the scale of digital native based on Don Tapscott(2008) including freedom, customization, investigation, integrity, entertainment, speed, and innovation. In addition, it was validated by collecting 506 data from Korea and China, and we confirmed the 8 dimension of digital native. There foung significant correltions between the tendency of digital native and IT consumption, especially, the factor of entertainment, freedom, and innovation showed higer correlation with IT consumption. The differences of digital native were found between Korean and Chinese. The marketing impications for digital native are suggested in conclusion.