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- 저자 : Jiehong Xie (검색결과 1 건)
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Kuo Zhang,Hong Huo,Yu Sun,Lunan Wang,Rui Zhang,Guigao Lin,Jiehong Xie,Qingtao Wang,Jinming Li 대한진단검사의학회 2014 Annals of Laboratory Medicine Vol.34 No.5
Background: The participation of laboratories in external quality assessment (EQA) pro- grams is required for the quality assurance of nucleic acid amplification of Chlamydia tra- chomatis . This study aimed to construct a new quality control (QC) material applicated in EQA of C. trachomatis PCR. Methods: A QC material-HTB-SiHa cells transfected with a recombinant plasmid contain- ing the cryptic plasmid sequence-was constructed for C. trachomatis PCR detection, and four different panels, each consisting of 4 positive samples with serial dilution of the con- structed QC material and 1 negative sample, were distributed by the National Center for Clinical Laboratories among four groups of 275, 268, 317, and 304 participants across China from 2011 through 2012. A total of eight commercial kits were used for C. tracho- matis PCR detection in participants. Results: Nine laboratories reported false-positive results (0.9%). As the series dilution in- creased, the correct reporting of the data sets decreased; the lowest correct rate was 96.3% in the weakest positive samples (10 4 copies/mL). Eight laboratories reported false- positive results, and 42 laboratories reported false-negative results in the EQA detection of C. trachomatis . No significant differences were observed in the detection of the con- structed C. trachomatis positive samples (97.9%, 98.5%, 100%, 98.5%; P =0.36) and negative samples (100%, 99.0%, 100%, 99.0%; P =0.764) using four commercial kits commonly used in China. Conclusions: The results of the EQA study indicated that the constructed material pro- vides a noninfectious, stable control material with sufficient volume for PCR detection of C. trachomatis.
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